scholarly journals Effect of Defatting and Extraction Solvent on the Antioxidant and Pancreatic Lipase Inhibitory Activities of Extracts from Hermetia illucens and Tenebrio molitor

Insects ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 789
Author(s):  
Joaquín Navarro del Hierro ◽  
Emma Cantero-Bahillo ◽  
Tiziana Fornari ◽  
Diana Martin
Keyword(s):  
Antioxidant Activity ◽  
Pancreatic Lipase ◽  
Methanol Extract ◽  
Inhibitory Effect ◽  
Extraction Yield ◽  
Edible Insects ◽  
Extraction Solvent ◽  
Bioactive Properties ◽  
Lipase A ◽  
Pre Treatment

The production of specific insect extracts with bioactive properties for human health is an emerging and innovative field for the edible insects industry, but there are unexplored extraction factors that might modulate the bioactivity of the extracts. Ultrasound-assisted extracts from T. molitor and H. illucens were produced. Effects of defatting pre-treatment and extraction solvent were evaluated on extraction yield, antioxidant activity and pancreatic lipase inhibitory effect. Chemical characterisation of defatted extracts from H. illucens was performed by GC-MS-FID. Non-defatted extracts showed higher extraction yields. Defatted extracts had similar extraction yields (around 3%). Defatted extracts had higher antioxidant activity, T. molitor being stronger than H. illucens. Antioxidant activity of T. molitor methanol extract was higher than the rest of solvents. Aqueous ethanol improved the antioxidant activity of H. illucens extracts. All extracts inhibited lipase, but no significant effect of defatting and solvent was observed for T. molitor. A significant higher inhibitory activity was observed for H. illucens, the strongest being defatted 100% and 70% ethanol H. illucens extracts. H. illucens extracts contained free amino acids and disaccharides, together with minor fractions of lipids, sterols and organic acids. These results evidence the potential of extracts obtained from edible insects as antioxidants and inhibitors of the pancreatic lipase, a simultaneous multibioactivity that might be favoured by the defatting pre-treatment of the samples and the solvent of extraction.

scholarly journals Characterization, antioxidant activity, and inhibitory effect on pancreatic lipase of extracts from the edible insects Acheta domesticus and Tenebrio molitor

2020 ◽  
Vol 309 ◽  
pp. 125742 ◽  
Author(s):  
Joaquín Navarro del Hierro ◽  
Alba Gutiérrez-Docio ◽  
Paz Otero ◽  
Guillermo Reglero ◽  
Diana Martin
Keyword(s):  
Antioxidant Activity ◽  
Pancreatic Lipase ◽  
Tenebrio Molitor ◽  
Inhibitory Effect ◽  
Acheta Domesticus ◽  
Edible Insects

scholarly journals IN VITRO ANTI-OBESITY EFFECT OF MACROLICHENS HETERODERMIA LEUCOMELOS AND RAMALINA CELASTRI BY PANCREATIC LIPASE INHIBITORY ASSAY

2017 ◽  
Vol 9 (5) ◽  
pp. 137
Author(s):  
Rashmi Shivanna ◽  
Hengameh Parizadeh ◽  
Rajkumar H. Garampalli
Keyword(s):  
Ethyl Acetate ◽  
Pancreatic Lipase ◽  
Methanol Extract ◽  
Well Being ◽  
Inhibitory Effect ◽  
Potential Effect ◽  
Protective Role ◽  
Treatment Of Obesity ◽  
Molecular Sequencing

Objective: Obesity is a chronic disorder caused by an imbalance between energy intake and expenditure in which excessive fat will be deposited in adipose tissue and poses a risk to the health and well-being of humans. Agents which inhibit pancreatic lipase play an important role in the treatment of obesity. The aim of this study was to assess the potential effect of macro lichens Heterodermia leucomelos (L.) Poelt a foliose lichen and Ramalina celastri (Sprengel) Krog and Swinscow a fruticose lichen in the treatment of obesity.Methods: In vitro anti-obesity inhibitory effect of macro lichens were evaluated by using chicken pancreatic lipase activity. Lipase was extracted from the chicken pancreas. Different concentrations from 5-25 mg/ml of methanol and ethyl acetate extracts of lichens Heterodermia leucomelos and Ramalina celastri was incubated with pancreas lipase.Results: With the increase in the concentration of extracts the higher inhibition of the enzyme was observed. Solvent methanol showed good activity compared to ethyl acetate. Percentage of inhibition ranged from 19.7-69.8 and 20.0-86.6 % in the methanol extract of Heterodermia leucomelos and Ramalina celastri respectively. Comparatively lichen Ramalina celastri in methanol extract showed maximum inhibition of 86.6 %, whereas ethyl acetate showed an inhibition of 63.0% at 25 mg/ml against enzyme lipase.Conclusion: In the present study, the inhibitory activity of lichen indicates its protective role in treating obesity. Molecular sequencing of this lichen helps in future to determine the various metabolic pathways that are responsible for the production of novel compounds.

scholarly journals Study on Efficacy Evaluation of Deramal Bioactive Properties of the Prunus tomentosa Fruits Fermented with Lactobacillus acidphilus

2021 ◽  
Vol 27 (4) ◽  
pp. 949-956
Author(s):  
Da-Bin Jung ◽  
Hwa-Jung Choi ◽  
Mi-Young Yun
Keyword(s):  
Antioxidant Activity ◽  
Collagen Synthesis ◽  
Dermal Fibroblast ◽  
Inhibitory Effect ◽  
Melanoma Cells ◽  
Tyrosinase Activity ◽  
No Production ◽  
Bioactive Properties ◽  
Dpph Method ◽  
Anti Oxidant

The materials fermented with natural products have been used in a variety of cosmetic applications as a whitening, wrinkle and inflammatory agent. <i>Prunus tomentosa</i> fruits have been used as a traditional medicine for anti-oxidant and have been reported to possess anti-microbial properties. This study investigated the deramal bioactive properties of the <i>Prunus tomentosa</i> fruits fermented with <i>Lactobacillus acidphilus</i> (PTF). The antioxidant activity of the PTF was measured by 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. The cytotoxicity of human dermal fibroblast cell, murine macrophage cell line RAW 264.7 and B16-F10 melanoma cells were evaluated by sulforhodamine B (SRB) assay. Inhibitory effect of the PTF on nitric oxide (NO) prodction was evaluated by Griess Reagent System. The collagen synthesis was evaluated by using procollagen type-IC peptide (PIP) EIA kit. The tyrosinase activity, inhibitory effect of elastase and tumor necrosis factor-α (TNF-α) induced matrixmelloproteinase-1 (MMP-1) were also evaluated. It found that the PTF showed antioxidant activity without showing significant cytotoxicity in tested cells. The PTF were downregulated NO production in LPS-stimulated macrophages with nocytotoxicity (p<0.05). The PTF significantly induced collagen synthesis from the skin fibroblasts (p<0.05) and also dramatically inhibited MMP-1 activity (p<0.05). The PTF were inhibited activity of elastase and dramatically decreased melanin synthesis with inhibition of tyrosinase activity (p<0.05) and didn’t show cytotoxicity in B16-F10 melanoma cells. Therefore, the PTF showed bioactive properties on anti-oxidant, whitening, skin elasticity and anti-wrinkle efficacy. The PTF may be an appropriate choice for cosmetic products that aim to provide dermal bioactive effects.

scholarly journals ANTI-PROLIFERATIVE ACTIVITY OF ZIZYPHUS SPINA CHRISTI LEAVES METHANOL EXTRACT AGAINST RHABDOMYOSARCOMA (RD) CELL LINE

2017 ◽  
Vol 9 (2) ◽  
pp. 279 ◽  
Author(s):  
Ahmed R. Abu-raghif ◽  
Ghaith A. Jasim ◽  
Muneer M. Hanoon
Keyword(s):  
Antioxidant Activity ◽  
Cell Line ◽  
Proliferative Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Extraction Yield ◽  
Gas Chromatography Mass Spectrometry ◽  
Scavenging Activity

<p><strong>Objective: </strong>The aim of this study was to investigate the capability of <em>Zizyphus spina christi</em> methanol extract to inhibit cancer cell line proliferation.</p><p><strong>Methods: </strong>The leaves of <em>Zizyphus spina christi</em> were extracted by cold maceration method. The anti-proliferative activity of the methanol extract against rhabdomyosarcoma (RD) cell line was tested by 3-(4, 5 Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The phytochemical constituents were identified by gas chromatography-mass spectrometry (GC-MS) analysis. The antioxidant activity was assessed by measuring free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. <strong></strong></p><p><strong>Results: </strong>The percentage extraction yield for leaves with methanol was 20.64%. The methanol extract showed dose dependent inhibition of RD cell line, the IC<sub>50</sub> was 154.44 µg/ml. GC-MS showed the presence of flavonoid fraction and other compounds with antioxidant activity. The methanol extract demonstrated DPPH scavenging activity with IC<sub>50</sub> of 33.91 mg/ml.</p><p><strong>Conclusion: </strong>Methanol extract showed<strong> </strong>potential anti-proliferative activity against RD cell line,<strong> </strong>which could be due to its antioxidant activity.</p>

Selective Inhibition of Thromboxane Synthetase with Dazoxiben - Basis of Its Inhibitory Effect on Platelet Adhesion

1983 ◽  
Vol 49 (02) ◽  
pp. 096-101 ◽  
Author(s):  
V C Menys ◽  
J A Davies
Keyword(s):  
Arachidonic Acid ◽  
Platelet Adhesion ◽  
Fold Increase ◽  
Selective Inhibition ◽  
Inhibitory Effect ◽  
Selective Inhibitor ◽  
Coated Glass ◽  
Thromboxane Synthetase ◽  
Pre Treatment

SummaryPlatelet adhesion to rabbit aortic subendothelium or collagen-coated glass was quantitated in a rotating probe device by uptake of radio-labelled platelets. Under conditions in which aspirin had no effect, dazoxiben, a selective inhibitor of thromboxane synthetase, reduced platelet adhesion to aortic subendothelium by about 40% but did not affect adhesion to collagen-coated glass. Pre-treatment of aortic segments with 15-HPETE, a selective inhibitor of PGI2-synthetase, abolished the inhibitory effect of dazoxiben on adhesion. Concentrations of 6-oxo-PGFlα in the perfusate were raised in the presence of dazoxiben alone, and following addition of thrombin (10 units/ml) there was a 2-3 fold increase in concentration. Perfusion of damaged aorta with platelets labelled with (14C)-arachidonic acid in the presence of thrombin and dazoxiben resulted in the appearance of (14C)-labelled-6-oxo-PGFiα. Inhibition of thromboxane synthetase limits platelet adhesion probably by promoting vascular synthesis of PGI2 from endoperoxides liberated from adherent platelets, which subsequently promotes detachment of cells from the surface.

Uşak İli’nden Toplanan Taxus Baccata (L) Türünün Metanol Ekstresinin Yaprak, Dal ve Meyvesinde Biyolojik Aktivitenin Belirlenmesi

2019 ◽  
Vol 7 (10) ◽  
pp. 1533
Author(s):  
Yasemin Karafakıoglu
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Maximum Amount ◽  
Chemical Components ◽  
Taxus Baccata ◽  
Dpph Radical ◽  
Flavonoid Content ◽  
Future Studies ◽  
Different Parts

Taxus species in different parts of the world prevent the formation of hepatoprotective, anticoagulant, antiulcerogenic, anti-coagulant, antifungal and tumour cells. In this study, biological activity studies were performed on the leaves, branches and fruits of the methanol extract of Taxus baccata L. species collected from Sivaslı district of Uşak province. The methanol extract of the T.baccata species, the total amount of phenolic content of the sample was found to be 14.76 mg GAEQ/1g dry sample in the highest leaves. In T.baccata methanol extract, the maximum amount of flavonoid content was 0.468±mg QE/g dry sample. Antioxidant activity value as DPPH radical; % inhibition value was found as 93.21%. Based on the results; It can be concluded that the leaves of T.baccata have higher antioxidant activity than fruits and branches. Future studies need to explore the chemical components contained in the T.baccata species to determine and investigate in further detail.

Effect of Ethanol Treatment on Lycopene Extraction yield

2018 ◽  
Author(s):  
Nam Kyong-il ◽  
KIM rak-chon ◽  
Kang chang-hyok ◽  
Lee song-nam ◽  
Ryom sok-hun
Keyword(s):  
Antioxidant Activity ◽  
Treatment Time ◽  
Extraction Yield ◽  
Extraction Rate ◽  
Test Results ◽  
Ethanol Treatment ◽  
Optimum Conditions ◽  
Liquid Ratio ◽  
Pretreatment Method ◽  
Ethanol Pretreatment

In order to extract lycopene more effectively, this experiment focused on the optimization of ethanol pretreatment method to study the effects of ethanol treatment on the extraction rate of lycopene and its antioxidant activity. The test results show that 2 times ethanol treatment is very effective for improving lycopene yield. The optimum conditions of ethanol treatment are temperature 50 ℃, treatment time 1 time 2h, 2 times 2h, solid to liquid ratio is 1:12. The lycopene yield can be reached 20mg / 100g above.

Curcumin-based antioxidant and glycohydrolase inhibitor compounds: Synthesis and in vitro appraisal of the dual activity against diabetes

2020 ◽  
Vol 16 ◽  
Author(s):  
Sajjad Esmaeili ◽  
Nazanin Ghobadi ◽  
Donya Nazari ◽  
Alireza Pourhossein ◽  
Hassan Rasouli ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Enzyme Inhibitor ◽  
Radical Scavenging Activity ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Inhibitory Effect ◽  
Curcumin Derivatives ◽  
Reducing Power Assay

Background: Curcumin, as the substantial constituent of the turmeric plant (Curcuma longa), plays a significant role in the prevention of various diseases, including diabetes. It possesses ideal structure features as enzyme inhibitor, including a flexible backbone, hydrophobic nature, and several available hydrogen bond (H-bond) donors and acceptors. Objective: The present study aimed at synthesizing several novel curcumin derivatives and further evaluation of these compounds for possible antioxidant and anti-diabetic properties along with inhibitory effect against two carbohydrate-hydrolyzing enzymes, α-amylase and α-glucosidase, as these enzymes are therapeutic targets for attenuation of postprandial hyperglycemia. Methods: Therefore, curcumin-based pyrido[2,3-d]pyrimidine derivatives were synthesized and identified using an instrumental technique like NMR spectroscopy and then screened for antioxidant and enzyme inhibitory potential. Total antioxidant activity, reducing power assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH• ) radical scavenging activity were done to appraisal the antioxidant potential of these compounds in vitro. Results: Compounds L6-L9 showed higher antioxidant activity while L4, L9, L12 and especially L8 exhibited the best selectivity index (lowest α-amylase/α-glucosidase inhibition ratio). Conclusion: These antioxidant inhibitors may be potential anti-diabetic drugs, not only to reduce glycemic index but also to limit the activity of the major reactive oxygen species (ROS) producing pathways.

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