scholarly journals Phylogeny of Anopheles (Kerteszia) (Diptera: Culicidae) Using Mitochondrial Genes

Insects ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 324 ◽  
Author(s):  
Karin Kirchgatter ◽  
Lilian de Oliveira Guimarães ◽  
Henrry Hugo Yañez Trujillano ◽  
Fernando Rafael Arias ◽  
Abraham Cáceres ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Species Recognition ◽  
Phylogenetic Analyses ◽  
Mosquito Species ◽  
Relevant Information ◽  
Neotropical Region ◽  
Similar Species ◽  
Serra Do Mar ◽  
Additional Tool ◽  
First Time

Identification of mosquito species is necessary for determining the entomological components of malaria transmission, but it can be difficult in morphologically similar species. DNA sequences are largely used as an additional tool for species recognition, including those that belong to species complexes. Kerteszia mosquitoes are vectors of human and simian malaria in the Neotropical Region, but there are few DNA sequences of Kerteszia species in public databases. In order to provide relevant information about diversity and improve knowledge in taxonomy of Kerteszia species in Peru, we sequenced part of the mitochondrial genome, including the cytochrome c oxidase I (COI) barcode region. Phylogenetic analyses structured all species of mosquitoes collected in Peru into a single clade, separate from the Brazilian species. The Peruvian clade was composed of two lineages, encompassing sequences from Anopheles (Kerteszia) boliviensis and Anopheles (Kerteszia) pholidotus. An. pholidotus sequences were recorded for the first time in Peru, whereas An. boliviensis sequences were for the first time published in the GenBank database. Sequences generated from specimens morphologically identified as Anopheles (Kerteszia) cruzii clustered into three separate clades according to the collection localities of Serra do Mar, Serra da Mantiqueira, and Serra da Cantareira, confirming An. cruzii as a species complex, composed of at least three putative species.

scholarly journals Evaluating methodologies for species delimitation: the mismatch between phenotypes and genotypes in lichenized fungi (Bryoria sect. Implexae, Parmeliaceae)

2019 ◽  
Vol 42 (1) ◽  
pp. 75-100 ◽  
Author(s):  
C.G. Boluda ◽  
V.J. Rico ◽  
P.K. Divakar ◽  
O. Nadyeina ◽  
L. Myllys ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Species Delimitation ◽  
Sequence Data ◽  
Species Recognition ◽  
Phylogenetic Analyses ◽  
Phylogenetic Reconstruction ◽  
Haplotype Network ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Molecular Sequence Data

In many lichen-forming fungi, molecular phylogenetic analyses lead to the discovery of cryptic species within traditional morphospecies. However, in some cases, molecular sequence data also questions the separation of phenotypically characterised species. Here we apply an integrative taxonomy approach – including morphological, chemical, molecular, and distributional characters – to re-assess species boundaries in a traditionally speciose group of hair lichens, Bryoria sect. Implexae. We sampled multilocus sequence and microsatellite data from 142 specimens from a broad intercontinental distribution. Molecular data included DNA sequences of the standard fungal markers ITS, IGS, GAPDH, two newly tested loci (FRBi15 and FRBi16), and SSR frequencies from 18 microsatellite markers. Datasets were analysed with Bayesian and maximum likelihood phylogenetic reconstruction, phenogram reconstruction, STRUCTURE Bayesian clustering, principal coordinate analysis, haplotype network, and several different species delimitation analyses (ABGD, PTP, GMYC, and DISSECT). Additionally, past population demography and divergence times are estimated. The different approaches to species recognition do not support the monophyly of the 11 currently accepted morphospecies, and rather suggest the reduction of these to four phylogenetic species. Moreover, three of these are relatively recent in origin and cryptic, including phenotypically and chemically variable specimens. Issues regarding the integration of an evolutionary perspective into taxonomic conclusions in species complexes, which have undergone recent diversification, are discussed. The four accepted species, all epitypified by sequenced material, are Bryoria fuscescens, B. glabra, B. kockiana, and B. pseudofuscescens. Ten species rank names are reduced to synonymy. In the absence of molecular data, they can be recorded as the B. fuscescens complex. Intraspecific phenotype plasticity and factors affecting the speciation of different morphospecies in this group of Bryoria are outlined.

Monograph of Elaphoglossum sect. Polytrichia subsect. Apoda (Dryopteridaceae)

2021 ◽  
Author(s):  
Fernando B. Matos ◽  
Josmaily L&#243riga ◽  
Robbin C. Moran
Keyword(s):  
Phylogenetic Analyses ◽  
Morphological Characters ◽  
Character State ◽  
Similar Species ◽  
Southern Mexico ◽  
Distribution Maps ◽  
Molecular Phylogenetic ◽  
Identification Of Species ◽  
First Time ◽  
A New Species

We present a monographic treatment for the 13 species of Elaphoglossum sect. Polytrichia subsect. Apoda (Dryopteridaceae). Molecular phylogenetic analyses have recovered this subsection as monophyletic within the so-called “subulate-scaled clade” of Elaphoglossum. Morphologically, the species of E. subsect. Apoda are usually characterized by the presence of brightly colored stem scales (yellowish, orangish, orreddish, as opposed to castaneous to black), evenly distributed subulate scales on laminar surfaces, tiny glandular hairs on different parts of the leaves, and adult sterile leaves without hydathodes. The species can be divided into two groups: one with subsessile and the other with long-petiolate leaves. Elaphoglossum subsection Apoda is distributed from southern Mexico and the Antilles to Bolivia and midwestern Brazil. Elaphoglossum atehortuae, a new species from the Amazon region of Ecuador, is described, illustrated, and compared to its most similar species. It is unusual within this group for lacking subulate scales on both surfaces of the laminae, a character state that it shares with E. backhouseanum. Two species, E. polyblepharum and E. trichophorum, are included in the group for the first time, whereas E. procurrens and E. siliquoides are excluded based on morphological characters and previously published molecular phylogenies. We also provide comments for E. ×morphohybridum, which is a hybrid between E. alvaradoanum and E. crinitum. Lectotypes are designated for Acrostichum apodum, A. platyneuron, A. cubense, A. procurrens, A. trichophorum, and Elaphoglossum auripilum, and epitypes are designated for A. apodum. To facilitate the identification of species, we provide keys, descriptions, illustrations, comments, synonyms, distribution maps, spore images, and a list of specimens examined.

Taxonomy and the unusual morphological similarity among Hypocerides Schmitz species (Diptera: Phoridae)

Zootaxa ◽  
2021 ◽  
Vol 4980 (1) ◽  
pp. 113-126
Author(s):  
DANILO CÉSAR AMENT
Keyword(s):  
Species Recognition ◽  
Morphological Characters ◽  
Neotropical Region ◽  
Morphological Similarity ◽  
Worldwide Distribution ◽  
New Material ◽  
Biogeographic Regions ◽  
Males And Females ◽  
Morphological Differences ◽  
First Time

Hypocerides Schmitz comprises seven species of considerable morphological similarity according to authors that described or reviewed material from the genus. This lack of variation is problematic for its taxonomy as little is known about which structures could be evidence for species recognition or even about which sex is the most informative to its species-level taxonomy. In this study, I examined males and females of the genus from the Neotropical, Nearctic, and Palearctic Regions in search of informative morphological characters for the genus taxonomy. The examination included types of five of the seven known species and new material from the Neotropical Region and Madagascar. I present a detailed description of the genus, illustrate some of its structures for the first time, and compare the morphology of the specimens examined. Few morphological differences were found but none was considered sufficient to infer species limits. This morphological uniformity among species from different biogeographic regions can be seen as evidence that they could all be one species with an almost worldwide distribution. The low morphological variation among Hypocerides species and their apparent wide distributions remain interesting biological questions. 

Disentangling the evolutionary and biogeographical history of the freshwater fish trematode genus Creptotrema (Digenea: Allocreadiidae) using an integrative taxonomy approach: the case of Creptotrema agonostomi in Middle American mountain mullets

2020 ◽  
Vol 94 ◽  
Author(s):  
G. Pérez-Ponce de León ◽  
A.L. Sereno-Uribe ◽  
M. García-Varela ◽  
B. Mendoza-Garfias ◽  
D.I. Hernández-Mena ◽  
...  
Keyword(s):  
New York ◽  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
New York State ◽  
Integrative Taxonomy ◽  
Similar Species ◽  
The North ◽  
Middle America ◽  
Molecular Phylogenetic ◽  
Three New Species

Abstract Species of the allocreadiid genus Creptotrema are parasites of freshwater fishes in the Americas. Species in the genus possess one pair of muscular oral lobes on the oral sucker. Currently, the genus contains eight species, six distributed in South America, one in Middle America and one in North America. Genetic data are only available for the North American species, Creptotrema funduli, a parasite of fundulids originally described from Oneida Lake, New York State. In this study, we obtained 28S ribosomal DNA sequences of trematodes morphologically similar to Creptotrema agonostomi from the mountain mullet, Dajaus monticola, across a wide geographical range in Middle America. Our molecular phylogenetic analyses showed that (1) the genus Creptotrema, as currently conceived, is not monophyletic; (2) the allocreadiids in mountain mullets should be re-allocated in the genus Pseudoparacreptotrema; and (3) the allocreadiid trematodes from D. monticola across Middle America represent four morphologically similar species, three of which can be distinguished genetically. These three new species are described herein using an integrative taxonomy approach. We contend that accurate estimates of species diversity and phylogenetic relationships among allocreadiids, and most likely other species of trematodes, necessarily require an integrative taxonomy approach that should consider at least DNA sequences and scanning electron microscopy.

A new perennial Erysimum species from Turkey, E. nemrutdaghense (Brassicaceae)

Phytotaxa ◽  
2018 ◽  
Vol 336 (3) ◽  
pp. 239 ◽  
Author(s):  
BİROL MUTLU
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Its Region ◽  
Nuclear Ribosomal Dna ◽  
Similar Species ◽  
Sem Images ◽  
First Time ◽  
East Anatolia ◽  
A New Species ◽  
Different Parts

In this study, Erysimum nemrutdaghense Mutlu (Brassicaceae) is described as a new species from the province of Adıyaman in the South-east Anatolia. In order to determine the phylogenetic relationships of the new species, a portion of the nuclear ribosomal DNA sequences of ITS region was used. In addition to the new species, ITS regions of five Erysimum species were sequenced. Erysimum sintenisianum Bornm., E. lazistanicum (Rupr.) Lipsky, and E. stenophyllum Polatschek were sequenced in this study for the first time. The diagnostic morphological and phylogenetic characteristics are discussed with similar species. SEM images of trichomes on different parts of siliqua, stigma, and seed are given. Chromosome number of the new species is found to be 2n=16.

Phylogeny of Cladonia uncialis (Cladoniaceae, Lecanoromycetes) and its allies

2015 ◽  
Vol 47 (4) ◽  
pp. 215-231 ◽  
Author(s):  
Soili Stenroos ◽  
Raquel Pino-Bodas ◽  
Diana Weckman ◽  
Teuvo Ahti
Keyword(s):  
Dna Sequences ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Usnic Acid ◽  
Eastern United States ◽  
Dna Sequence Data ◽  
Rdna Igs ◽  
Alternative Topology ◽  
Reindeer Lichens ◽  
First Time

AbstractThe species from Cladonia section Unciales are characterized by the absence of squamules and soredia on the corticate podetia and the presence of usnic acid. Different subspecies, varieties and forms have been distinguished in the type species C. uncialis. In this study, a molecular phylogeny of Cladonia uncialis and members of the traditionally recognized section Unciales, along with additional potentially allied species, was constructed. DNA sequences from three gene loci, namely ITS rDNA, IGS rDNA and ß-tubulin, were analyzed using Maximum Parsimony, Maximum Likelihood, and Bayesian methods. Eleven species were analyzed for the first time using DNA sequence data. Cladonia uncialis subsp. uncialis and subsp. biuncialis were recognized as distinct taxa. The recognition of C. pseudostellata as a species was not supported by the analyses, but it represents a hypothamnolic acid chemotype, which is reported here as new to Europe (Scotland). The presence of subsp. biuncialis in North America (Newfoundland) was substantiated. The subsp. uncialis usually lacks squamatic acid, but in the eastern United States a morph referred to as subsp. uncialis does normally contain that acid. However, this morph did not attain taxonomic recognition based on phylogenetic analyses. All the other taxa formerly included in sect. Unciales turned out to belong to other groups of Cladonia, mainly Amaurocraeae, Borya, Divaricatae, and Perviae. The formerly recognized genus Cladina (reindeer lichens) is non-monophyletic, consisting of three groups within Cladonia, making the concept Cladina even nomenclaturally useless. Alternative topology tests rejected the monophyly of C. pseudostellata, section Unciales and Cladina.

scholarly journals Detection of the Invasive Mosquito Species Aedes (Stegomyia) aegypti and Aedes (Hulecoeteomyia) koreicus on the Southern Coast of the Crimean Peninsula

2020 ◽  
Author(s):  
Lyudmila Ganushkina ◽  
Alexander Lukashev ◽  
Ivan Patraman ◽  
Vladimir Razumeyko ◽  
Еlena Shaikevich
Keyword(s):  
Dna Sequences ◽  
Mosquito Species ◽  
Southern Coast ◽  
Single Male ◽  
Crimean Peninsula ◽  
Vector Borne Diseases ◽  
Invasive Mosquito Species ◽  
Invasive Mosquito ◽  
First Time ◽  
The Crimean Peninsula

Background: The incidence and area of arbovirus infections is increasing around the world. It is largely linked to the spread of the main arbovirus vectors, invasive mosquito of the genus Aedes. Previously, it has been reported that Aedes aegypti reemerged in Russia after a 50-year absence. Moreover, in 2011, Ae. albopictus was registered in the city of Sochi (South Russia, Black Sea coast) for the first time. In 2013, Asian Ae. koreicus was found in Sochi for the first time. Methods: Mosquitoes were collected using the following methods: larvae with a dip net, imago on volunteers and using bait traps. The mosquitoes were identified using both morphology and sequencing of the second internal transcribed spacer of the nuclear ribosomal RNA gene cluster. Results: In August 2016, Ae. koreicus larvae and imago and a single male of Ae. aegypti were found on the southern coast of the Crimean Peninsula, where they were not registered before. Newly obtained DNA sequences were registered in GenBank with the accession numbers MF072936 and MF072937. Conclusion: Detection of invasive mosquito species (Ae. aegypti and Ae. koreicus) implies the possibility of their area expansion. Intensive surveillance is required at the Crimean Peninsula to evaluate the potential for the introduction of vector-borne diseases.

scholarly journals Additions to Chaetothyriaceae (Chaetothyriales): Longihyalospora gen. nov. and Ceramothyrium longivolcaniforme, a new host record from decaying leaves of Ficus ampelas

MycoKeys ◽  
2019 ◽  
Vol 61 ◽  
pp. 91-109 ◽  
Author(s):  
Danushka S. Tennakoon ◽  
Kasun M. Thambugala ◽  
Rajesh Jeewon ◽  
Sinang Hongsanan ◽  
Chang-Hsin Kuo ◽  
...  
Keyword(s):  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Host Record ◽  
Bootstrap Support ◽  
Similar Species ◽  
New Host ◽  
New Host Record ◽  
Distinct Genus ◽  
High Bootstrap ◽  
First Time

A novel ascomycete genus, Longihyalospora, occurring on leaf litter of Ficus ampelas in Dahu Forest Area in Chiayi, Taiwan is described and illustrated. Longihyalospora is characterized by dark mycelium covering the upper leaf surface, elongate mycelial pellicle with ring of setae, pale brown to brown peridium, broadly obovoid, short pedicellate asci and hyaline, fusiform, elongated (tapering ends) and multi-septate ascospores with a thin mucilaginous sheath. Phylogenetic analyses of combined ITS, LSU and SSU sequence data revealed Longihyalospora as a distinct genus within the Chaetothyriaceae with high bootstrap support. Moreover, based on morphological similarities, Chaetothyrium vermisporum transferred to the new genus. In addition, Ceramothyrium longivolcaniforme is reported for the first time on Ficus ampelas. Newly added species are compared with other similar species and comprehensive descriptions and micrographs are provided.

scholarly journals Schroeteria Decaisneana, S. Poeltii, and Ciboria Ploettneriana (Sclerotiniaceae, Helotiales, Ascomycota), Three Parasites on Veronica Seeds: First Report of Teleomorphs in Schroeteria

2021 ◽  
Author(s):  
Hans-Otto Baral ◽  
Peter Rönsch ◽  
Udo Richter ◽  
Alexander Urban ◽  
Julia Kruse ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Similar Species ◽  
Protein Coding ◽  
Ultrastructural Studies ◽  
The Core ◽  
Rdna Sequences ◽  
False Smut ◽  
Relationship Of ◽  
First Time ◽  
The Relationship

Abstract Three little known, morphologically similar species of Sclerotiniaceae which form their apothecia on fallen stromatized Veronica seeds are described and illustrated in detail based on fresh collections or moist chamber cultures of infected seeds: Ciboria ploettneriana, Schroeteria decaisneana, and Schroeteria poeltii. The former two were found on Veronica hederifolia agg. at different sites of temperate central Europe, the latter on V. cymbalaria in a mediterranean region of Spain. The latter two are anamorph-typified and here reported for the first time with their teleomorph.Ciboria ploettneriana was described by Kirschstein as Sclerotiniaploettneriana on seeds of V. hederifolia agg. but is currently treated in Ciboria. Based on the reexamination of four syntype specimens in B it became evident that Kirschstein confused the two species on V. hederifolia. A lectotype is therefore designated for S. ploettneriana.Members of Schroeteria are specific plant parasites infecting fruits of different Veronica spp. Schroeteria has earlier been referred to the Ustilaginales (Basidiomycota) based on its smut-like chlamydospores, but later light-microscopic and ultrastructural studies suggested that it represents a false smut fungus belonging to the Sclerotiniaceae (Helotiales).rDNA sequences were obtained from chlamydospores of Schroeteria bornmuelleri (on V. rubrifolia), S. delastrina (generic type, on Veronica arvensis), S. decaisneana, and S. poeltii, and from apothecia on V. hederifolia agg. and V. cymbalaria seeds. As a result, the anamorph-teleomorph connection could be verified for Schroeteria decaisneana and S. poeltii based on a 100% ITS similarity between both morphs, whereas Ciboria ploettneriana in the here redefined sense could not be connected to an anamorph.Our phylogenetic analyses show that Ciboria ploettneriana belongs in the relationship of Sclerotinia, Stromatinia, and Grovesinia rather than Ciboria, but its placement was not supported. Also Schroeteria poeltii clustered unresolved in this relationship but has a much higher molecular distance to those. The remaining three Schroeteria spp. formed a supported monophyletic group, the Schroeteria core clade, which clustered with medium to low support distantly to a member of the Monilinia alpina group of section Disjunctoriae (M. jezoensis). ITS distances of 5–6.3% were found among members of the Schroeteria core clade, and 13.8–14.7% between the core clade and S. poeltii. The high distance of S. poeltii reflects its deviating chlamydospore morphology.Despite the high heterogeneity in the available ITS and LSU data, Schroeteria is accepted here under inclusion of S. poeltii as a genus distinct from Monilinia, particularly because of its very special anamorphs. A similar heterogeneity in rDNA analyses was observed in Monilinia and other genera of Sclerotiniaceae. Protein-coding genes should be investigated in order to obtain a more natural phylogeny within the Sclerotiniaceae.

A morphological and molecular study of adults and metacercariae of Hysteromorpha triloba (Rudolpi, 1819), Lutz 1931 (Diplostomidae) from the Neotropical region

2018 ◽  
Vol 93 (1) ◽  
pp. 91-99 ◽  
Author(s):  
A.L. Sereno-Uribe ◽  
A. López-Jimenez ◽  
L. Andrade-Gómez ◽  
M. García-Varela
Keyword(s):  
Dna Sequences ◽  
Developmental Stages ◽  
Phylogenetic Analyses ◽  
Mitochondrial Gene ◽  
Molecular Study ◽  
Neotropical Region ◽  
The Body ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Nearctic Region

AbstractAdults of Hysteromorpha triloba (Rudolpi, 1819), Lutz, 1931 inhabit primarily the intestine of cormorants across the globe, whereas metacercariae have been found in the body cavity of freshwater fishes of the families Cyprinidae, Ictaluridae, Ariidae, Pimelodidae and Catostomidae. In this study, adults and metacercariae identified as H. triloba were collected from the Neotropical cormorant (Nannopterum brasilianus) and from the Mexican tetra fish (Astyanax mexicanus) from the Gulf of Mexico and Pacific Ocean slopes in the Neotropical region. Partial DNA sequences of the mitochondrial gene cytochrome c oxidase subunit I (cox 1) and the internal transcribed spacers (ITS1, 5.8S and ITS2) of nuclear ribosomal DNA were generated for both developmental stages, and were compared with available sequences of H. triloba from the Nearctic region. The genetic divergence between metacercariae and adults of H. triloba from the Neotropical and Nearctic region (Canada) associated with the double-crested cormorant (Nannopterum auritus), ranged from 0 to 5.5% for cox 1 and from 0 to 0.2% for ITS. Phylogenetic analyses inferred with both molecular markers using maximum likelihood and Bayesian inference placed the adults and metacercariae in a single clade, confirming that both stages are conspecific. Our data confirmed that H. triloba is a widely distributed species across the Americas, parasitizing both the Neotropical and Nearctic cormorants in Argentina, Brazil, Venezuela, Mexico, USA and Canada.

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