scholarly journals A Gustatory Receptor GR8 Tunes Specifically to D-Fructose in the Common Cutworm Spodoptera litura

Insects ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 272 ◽  
Author(s):  
Xiao-Long Liu ◽  
Qi Yan ◽  
Yi-Lin Yang ◽  
Wen Hou ◽  
Chun-Li Miao ◽  
...  
Keyword(s):  
Spodoptera Litura ◽  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Temporal Expression ◽  
Common Cutworm ◽  
Proboscis Extension ◽  
Multiple Behaviors ◽  
The Common ◽  
Functional Analyses ◽  
Higher Sensitivity

Gustatory receptors (GRs) are crucial in the peripheral coding of the non-volatile compounds in insects, and thus play important roles in multiple behaviors including feeding, mating, and oviposition. However, little research has been done on GRs in lepidopteran pests. In the current work with Spodoptera litura, an important worldwide crop’s pest, a candidate fructose GR gene (SlitGR8) was cloned in full length, and its spatial and temporal expression profiles were determined by quantitative real-time PCR (qPCR). It revealed that SlitGR8 was highly expressed in antennae of both male and female adults, as well as in larva of first, fifth and sixth instar. Functional analyses were further conducted using the Xenopus oocyte system. SlitGR8 responded specifically to D-fructose among 12 tested sugar compounds. In addition, the behavioral assay demonstrated that both female and male moths could respond with proboscis extension behavior to D-fructose applied onto the antenna, but females showed higher sensitivity than males. The results provide an important base for further elucidation of molecular mechanisms of gustation, and a potential target for development of feeding interfering technique in S. litura.

scholarly journals Effects of insecticides on the common cutworm, Spodoptera litura (Fabricius) by the artificial diet-dip method

1998 ◽  
Vol 40 (0) ◽  
pp. 139-140
Author(s):  
Yumiko Taniyama ◽  
Minoru Negoro ◽  
Shoichi Kobayashi ◽  
Toshikazu Yamaoka ◽  
Manabu Shibao ◽  
...  
Keyword(s):  
Spodoptera Litura ◽  
Artificial Diet ◽  
Common Cutworm ◽  
The Common

scholarly journals Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Protein 9 Mediated Knockout Reveals Functions of the yellow-y Gene in Spodoptera litura

2020 ◽  
Vol 11 ◽  
Author(s):  
Xiao-Long Liu ◽  
Wei-Kang Han ◽  
Long-Ji Ze ◽  
Ying-Chuan Peng ◽  
Yi-Lin Yang ◽  
...  
Keyword(s):  
Spodoptera Litura ◽  
Expression Profiles ◽  
Marker Gene ◽  
The Body ◽  
Minor Role ◽  
Temporal Expression ◽  
Low Levels ◽  
Fat Bodies ◽  
Pupal Cuticle

Yellow genes are thought to be involved in the melanin biosynthetic pathway and play a crucial role in pigmentation reactions in insects. However, little research has been done on yellow genes in lepidopteran pests. To clarify the function of one of the yellow genes (yellow-y) in Spodoptera litura, we cloned the full-length of yellow-y, and investigated its spatial and temporal expression profiles by quantitative real-time PCR (qPCR). It revealed that yellow-y was highly expressed in larva of fourth, fifth, and sixth instars, as well as in epidermis (Ep), fat bodies (FB), Malpighian tubes (MT), and midguts (MG) of the larvae; whereas it was expressed in very low levels in different tissues of adults, and was almost undetected in pupa. This expression profile suggests an important role of yellow-y in larvae, minor role in adults, and no role in pupae. To confirm this, we disrupted yellow-y using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system, and obtained G0 insects with mutation in yellow-y. The mutation in yellow-y clearly rendered the larvae body, a color yellower than that of wide type insects, and in addition, the mutation resulted in abnormal segmentation and molting for older larvae. The mutation of yellow-y also made various adult tissues (antennae, proboscis, legs, and wings) yellowish. However, the mutation had no effect on pigmentation of the pupal cuticle. Taken together, our study clearly demonstrated the role of yellow-y not only in the body pigmentation of larvae and adults, and but also in segmentation and molting of larvae, providing new insights into the physiology of larval development, as well as a useful marker gene for genome editing based studies.

scholarly journals Identification of transcriptome and fluralaner responsive genes in the common cutworm Spodoptera litura Fabricius, based on RNA-seq

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhong-Qiang Jia ◽  
Di Liu ◽  
Ying-Chuan Peng ◽  
Zhao-Jun Han ◽  
Chun-Qing Zhao ◽  
...  
Keyword(s):  
Spodoptera Litura ◽  
Rna Seq ◽  
Common Cutworm ◽  
The Common

scholarly journals Dispersal of the Common Cutworm, Spodoptera litura, Monitored by Searchlight Trap and Relationship with Occurrence of Soybean Leaf Damage

Insects ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 427
Author(s):  
Akira Otuka ◽  
Masaya Matsumura ◽  
Makoto Tokuda
Keyword(s):  
Linear Regression ◽  
Spodoptera Litura ◽  
Pheromone Trap ◽  
Leaf Damage ◽  
Coefficient Of Determination ◽  
Female Ratio ◽  
Common Cutworm ◽  
Advantages And Disadvantages ◽  
Soybean Leaf ◽  
The Common

The common cutworm, Spodoptera litura Fabricius (Lepidoptera: Noctuidae) is a major pest of soybean. Pheromone traps are used to monitor male adults, but the catch peaks do not always predict leaf damage in soybean fields. Thus, there is no accurate means of forecasting soybean damage, and insecticide is applied on the basis of farmers’ observations of actual damage in fields. To understand the occurrence of soybean leaf damage, the dispersal of S. litura in a soybean field in southwestern Japan was preliminarily investigated using a searchlight trap in comparison to a pheromone trap at one location, from August to mid-October in 2016–2018. To determine the relationship between pest arrival and crop damage, trap catch numbers and the number of soybean leaves damaged by 1st-instar larvae were examined by separately comparing raw numbers and cumulative numbers. The raw catch numbers of the two trap types in August and September 2016 and 2018 preceded subsequent damage peaks by an average of 5.3 days. This temporal difference coincided with the estimated duration of the egg stage plus an assumed mating period. Furthermore, the cumulative catch numbers of the two traps in August and September were linearly associated with cumulative damaged leaves in the same period in each year and in the three-year period. The coefficient of determination (R2) of linear regression between the cumulative catch numbers of the searchlight trap and the cumulative damaged leaves for the three-year period was much higher than that between the cumulative catch of the pheromone trap and cumulative damage. This suggests that soybean leaf damage is closely linked to the number of S. litura arrivals at the survey site. Thus, the searchlight trap captured S. litura arrivals better than the pheromone trap. As the linear regression function of the cumulative catch of the searchlight trap for the three-year period was tentatively correlated with a prefectural economic injury level for soybean fields, it might be feasible to predict S. litura-induced soybean damage using searchlight traps. The cumulative female catch number of the searchlight trap was also linearly associated with damaged leaves, but the coefficient of determination was generally lower than that with the cumulative total catch. The female ratio of searchlight trap catches in September was <0.5 in contrast to S. litura migrating overseas (>0.5). The advantages and disadvantages of the two trapping methods, as well as necessary further studies are discussed. Our findings provide a foundation for S. litura monitoring with searchlight traps.

Overseas migration of the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae), from May to mid-July in East Asia

2013 ◽  
Vol 48 (2) ◽  
pp. 131-140 ◽  
Author(s):  
Sumio Tojo ◽  
Masasuke Ryuda ◽  
Takeshi Fukuda ◽  
Tadashi Matsunaga ◽  
Dong-Ro Choi ◽  
...  
Keyword(s):  
East Asia ◽  
Spodoptera Litura ◽  
Common Cutworm ◽  
The Common ◽  
Lepidoptera Noctuidae
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