scholarly journals Expression Patterns, Molecular Characterization, and Response to Host Stress of CYP Genes from Phenacoccus solenopsis (Hemiptera: Pseudococcidae)

Insects ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 264
Author(s):  
Lingyu Xi ◽  
Dan Liu ◽  
Lei Ma ◽  
Ying Zhang ◽  
Ruirui Sheng ◽  
...  

The quarantine insect pest Phenacoccus solenopsis (Hemiptera: Pseudococcidae) has a broad host range and is distributed worldwide. Each year, P. solenopsis causes significant crop losses. The detoxification of various xenobiotic compounds involves the cytochrome P450 monooxygenase (CYP) superfamily of enzymes. However, the functions of CYPs in P. solenopsis are poorly understood. In the present study, P. solenopsis was reared from the egg to the adult stage on three host plants: Tomato, cotton, and hibiscus. Thirty-seven P. solenopsis CYP genes were identified and their phylogenetic relationships were analyzed. Eleven CYP genes (PsCYP4NT1, PsCYP4G219, PsCYP6PZ1, PsCYP6PZ5, PsCYP301B1, PsCYP302A1, PsCYP305A22, PsCYP315A1, PsCYP353F1, PsCYP3634A1, and PsCYP3635A2) were selected for quantitative real-time PCR analysis. The results demonstrated marked differences in CYP expression levels in P. solenopsis grown on different host plants. The results will aid the molecular characterization of CYPs and will increase our understanding of CYP expression patterns in P. solenopsis during development and growth on different hosts.

2010 ◽  
Vol 76 (7) ◽  
pp. 2280-2285 ◽  
Author(s):  
Adi Behar ◽  
Laurie J. McCormick ◽  
Steve J. Perlman

ABSTRACT Many species of Rickettsia are well-known mammalian pathogens transmitted by blood-feeding arthropods. However, molecular surveys are continually uncovering novel Rickettsia species, often in unexpected hosts, including many arthropods that do not feed on blood. This study reports a systematic molecular characterization of a Rickettsia infecting the psocid Liposcelis bostrychophila (Psocoptera: Liposcelidae), a common and cosmopolitan household pest. Surprisingly, the psocid Rickettsia is shown to be Rickettsia felis, a human pathogen transmitted by fleas that causes serious morbidity and occasional mortality. The plasmid from the psocid R. felis was sequenced and was found to be virtually identical to the one in R. felis from fleas. As Liposcelis insects are often intimately associated with humans and other vertebrates, it is speculated that they acquired R. felis from fleas. Whether the R. felis in psocids causes disease in vertebrates is not known and warrants further study.


Insects ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 42 ◽  
Author(s):  
Francesco Nugnes ◽  
Stefania Laudonia ◽  
Giovanni Jesu ◽  
Maurice Gerardus Maria Jansen ◽  
Umberto Bernardo ◽  
...  

After the first record in 2008 in Southeast Italy, the alien invasive and quarantine pest Aleurocanthus spiniferus (orange spiny whitefly—OSW) has gradually spread throughout Europe, infesting several new host plants in addition to the known hosts. Molecular characterization of some Italian populations and a newly found Albanian population highlighted two different haplotypes invading Europe, belonging to one of the haplogroups previously recorded in China. A predator was recorded for the first time in several fields in Italy in association with OSW and other whitefly species. It was successively identified through a morpho-molecular characterization as a Nearctic member of the tribe Serangiini, the ladybird beetle, Delphastus catalinae. This predator represents a promising biocontrol agent to manage A. spiniferus outbreaks in Italy and other invaded countries.


2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Jingwei Huang ◽  
Kang Xiong ◽  
Houshuang Zhang ◽  
Yanzhen Zhao ◽  
Jie Cao ◽  
...  

2012 ◽  
Vol 116 (2) ◽  
pp. 249-260 ◽  
Author(s):  
Lucas M. Abreu ◽  
Sarah S. Costa ◽  
Ludwig H. Pfenning ◽  
Jacqueline A. Takahashi ◽  
Thomas O. Larsen ◽  
...  

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