scholarly journals Molecular and Functional Characterization of Elicitor PeBC1 Extracted from Botrytis cinerea Involved in the Induction of Resistance against Green Peach Aphid (Myzus persicae) in Common Beans (Phaseolus vulgaris L.)

Insects ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 35 ◽  
Author(s):  
Abdul Basit ◽  
Abdul Hanan ◽  
Talha Nazir ◽  
Muhammad Majeed ◽  
Dewen Qiu
Keyword(s):  
Phaseolus Vulgaris ◽  
Botrytis Cinerea ◽  
Myzus Persicae ◽  
Insect Pests ◽  
Green Peach Aphid ◽  
Common Beans ◽  
Phaseolus Vulgaris L ◽  
Expression Levels ◽  
Temperature Regimes ◽  
Bean Plants

Elicitors are biofactors that induce resistance in plants against different insect pests. This in vitro study evaluated the impact of a novel elicitor protein PeBC1, extracted from a necrotrophic fungus Botrytis cinerea, on the development and fecundity parameters of green peach aphid (Myzus persicae) on common beans (Phaseolus vulgaris L.). Three different concentrations of PeBC1 elicitor (i.e., 33.56, 25.43, 19.33 µg mL−1) were applied at three different temperature regimes (i.e., 18, 21, and 25 °C). Elicitor treatments were applied topically on the bean plants at 3-leaf stage and newly emerged (0–6 h old) apterous adult aphids were exposed to these treated leaves. In addition to the biological parameters of aphids, the relative expression levels of key genes associated with jasmonic acid (JA) and salicylic acid (SA) plant defense pathways were also determined through RT-qPCR. Results of bioassays revealed that the application of PeBC1 elicitor protein exhibited pronounced and significant (p < 0.05) sub-lethal effects on green peach aphids. The fecundity was reduced and the nymphal development time was prolonged by different concentrations of PeBC1 elicitor and temperature regimes. Gene expression studies showed that the exogenous application of PeBC1 induced a significant upregulation of the expression levels of JA and SA pathway-associated genes in bean plants. As compared to control, elicitor-treated plants exhibited an induced resistance against aphids. Our findings suggest the potential use of PeBC1 elicitor protein in future bio-intensive management strategies against sap-sucking insect pests such as green peach aphids.

scholarly journals MAMP-triggered resistance induced by elicitor protein PeBA1 derived from Bacillus amyloliquefaciens NC6 in common bean (Phaseolus vulgaris L.) against green peach aphid (Myzus persicae Sulzer)

2020 ◽  
Vol 48 (2) ◽  
pp. 705-715
Author(s):  
Abdul BASIT ◽  
Tala NAZIR ◽  
Abdul HANAN ◽  
Muhammad Z. MAJEED ◽  
Muhammad FARHAN ◽  
...  
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Myzus Persicae ◽  
Bacillus Amyloliquefaciens ◽  
Insect Pests ◽  
Foliar Application ◽  
Green Peach Aphid ◽  
Management Tool ◽  
Expression Levels

Bacterial microbe-associated molecular patterns (MAMPs) play an important role in innate plant immunity. This in vitro study evaluated the putative role of protein elicitor PeBA1 derived from Bacillus amyloliquefaciens NC6 strain in eliciting induced resistance type responses in common bean (Phaseolus vulgaris) plants against green peach aphid Myzus persicae. Nymphal developmental time of aphids was significantly prolonged and the fecundity was significantly reduced by different concentrations of PeBA1 elicitor (i.e. 40.51, 24.91 and 16.38 µg mL-1) applied at three different temperature regimes (i.e. 21, 27 and 30 °C). Moreover, foliar application of PeBA1 elicitor protein strongly up-regulated the expression levels of salicylic acid (SA) pathway-associated genes, while the expression levels of jasmonic acid (JA) pathway-associated genes exhibited a moderate induction. Quantification by LC/MSMS revealed a linear increase of both SA and JA plant defense hormones along with the time of exposure. Our findings suggest that the bacterial elicitor protein PeBA1 could be used as an effective biological pest management tool against phloem-feeding insect pests such as green peach aphids M. persicae.

Bioaccessibility of Phenols in Common Beans (Phaseolus vulgaris L.) and Iron (Fe) Availability to Caco-2 Cells

2008 ◽  
Vol 56 (22) ◽  
pp. 10999-11005 ◽  
Author(s):  
José Moisés Laparra ◽  
Raymond P. Glahn ◽  
Dennis D. Miller
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Beans ◽  
Phaseolus Vulgaris L

QTL analyses for seed iron and zinc concentrations in an intra-genepool population of Andean common beans (Phaseolus vulgaris L.)

2010 ◽  
Vol 122 (3) ◽  
pp. 511-521 ◽  
Author(s):  
Matthew W. Blair ◽  
Carohna Astudillo ◽  
Judith Rengifo ◽  
Steve E. Beebe ◽  
Robin Graham
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Beans ◽  
Phaseolus Vulgaris L ◽  
Iron And Zinc ◽  
Zinc Concentrations

scholarly journals Attraction of Astylus variegatus (Germ.) (Coleoptera: Melyridae) by volatile floral attractants

2007 ◽  
Vol 64 (3) ◽  
pp. 305-307 ◽  
Author(s):  
Maurício Ursi Ventura ◽  
Tania Pereira ◽  
Daiane Heloisa Nunes ◽  
Iara Cintra de Arruda
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Beans ◽  
Phaseolus Vulgaris L ◽  
Release Rates ◽  
Plastic Strip ◽  
Feeding Stimulant ◽  
Laboratory Conditions ◽  
Diabrotica Speciosa ◽  
And Control ◽  
Lagenaria Vulgaris

The beetle Astylus variegatus (Germ.) (Coleoptera: Melyridae) is frequently found in flowers feeding on pollen. Responses of A. variegatus to volatile floral attractants were studied in common beans (Phaseolus vulgaris L.) fields. Traps originally designed to capture Diabrotica speciosa (Germ.) (Coleoptera: Chrysomelidae), consisted of plastic bottles (2 L) with 150 holes (5-mm diameter) yellow gold painted and containing inside a plastic strip (3.5 <FONT FACE=Symbol>´</FONT> 25 cm) with Lagenaria vulgaris (L.) powder (0.28% B cucurbitacin - feeding stimulant and arrestant for diabroticites) sprayed with carbaril insecticide. Treatments consisted of 1,4-dimethoxybenzene (one or two dispensers per trap), 1,4-dimethoxybenze + indole, 1,4-dimethoxybenzene + cinnameldehyde and control. Volatile average release rates (over ten days) was approximately 32 mg day-1 per dispenser under laboratory conditions. 1,4-dimethoxybenzene-lured traps caught significantly more beetles than the control, three and seven days after trap setting. Ten days after the onset of the experiment, there were no differences in number of beetles caught by treatments. Captures were higher in the 1,4-dimethoxybenzene + cinnamaldehyde treatment than in 1,4-dimethoxybenzene only in the first assessment. Adding indole to 1,4-dimethoxybenzene did not improve beetle captures.

scholarly journals Content of zinc, iron and their absorption inhibitors in Nicaraguan common beans (Phaseolus vulgaris L.)

2013 ◽  
Vol 136 (1) ◽  
pp. 87-93 ◽  
Author(s):  
M.R. Martinez Meyer ◽  
Aldo Rojas ◽  
Arja Santanen ◽  
F.L. Stoddard
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Beans ◽  
Phaseolus Vulgaris L

scholarly journals Soil Application of Effective Microorganisms (EM) Maintains Leaf Photosynthetic Efficiency, Increases Seed Yield and Quality Traits of Bean (Phaseolus vulgaris L.) Plants Grown on Different Substrates

2019 ◽  
Vol 20 (9) ◽  
pp. 2327 ◽  
Author(s):  
Marcello Iriti ◽  
Alessio Scarafoni ◽  
Simon Pierce ◽  
Giulia Castorina ◽  
Sara Vitalini
Keyword(s):  
Phaseolus Vulgaris ◽  
Photosystem Ii ◽  
Sandy Soil ◽  
Photosynthetic Efficiency ◽  
Quality Traits ◽  
Phaseolus Vulgaris L ◽  
Effective Microorganisms ◽  
Yield And Quality ◽  
Bean Plants

EM (effective microorganisms) is a biofertilizer consisting of a mixed culture of potentially beneficial microorganisms. In this study, we investigated the effects of EM treatment on leaf in vivo chlorophyll a fluorescence of photosystem II (PSII), yield, and macronutrient content of bean plants grown on different substrates (nutrient rich substrate vs. nutrient poor sandy soil) in controlled environmental conditions (pot experiment in greenhouse). EM-treated plants maintained optimum leaf photosynthetic efficiency two weeks longer than the control plants, and increased yield independent of substrate. The levels of seed nutritionally-relevant molecules (proteins, lipids, and starch) were only slightly modified, apart from the protein content, which increased in plants grown in sandy soil. Although EM can be considered a promising and environmentally friendly technology for sustainable agriculture, more studies are needed to elucidate the mechanism(s) of action of EM, as well as its efficacy under open field conditions.

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