scholarly journals Novel Cold-Adapted Recombinant Laccase KbLcc1 from Kabatiella bupleuri G3 IBMiP as a Green Catalyst in Biotransformation

2021 ◽  
Vol 22 (17) ◽  
pp. 9593
Author(s):  
Katarzyna M. Wiśniewska ◽  
Aleksandra Twarda-Clapa ◽  
Aneta M. Białkowska
Keyword(s):  
Sulfonic Acid ◽  
Laccase Activity ◽  
Sinapic Acid ◽  
Synthetic Dyes ◽  
Optimal Temperature ◽  
Green Catalyst ◽  
Cold Adapted ◽  
Molar Yield ◽  
Overlap Extension ◽  
Recombinant Laccase

Cold-adapted enzymes are useful tools in the organic syntheses conducted in mixed aqueous-organic or non-aqueous solvents due to their molecular flexibility that stabilizes the proteins in low water activity environments. A novel psychrophilic laccase gene from Kabatiella bupleuri, G3 IBMiP, was spliced by Overlap‑Extension PCR (OE-PCR) and expressed in Pichia pastoris. Purified recombinant KbLcc1 laccase has an optimal temperature of 30 °C and pH of 3.5, 5.5, 6.0, and 7.0 in the reaction with 2,2′‑azino‑bis(3‑ethylbenzothiazoline‑6‑sulfonic acid) (ABTS), guaiacol, sinapic acid, and syringaldazine, respectively. Moreover, laccase KbLcc1 is highly thermolabile, as it loses 40% of activity after 30 min at 40 °C and is inactivated at 50 °C after the same period of incubation. The new enzyme remained active with 1 mM of Ni2+, Cu2+, Mn2+, and Zn2+ and with 2 mM of Co2+, Ca2+, and Mg2+, but Fe2+ greatly inhibited the laccase activity. Moreover, 1% ethanol had no impact on KbLcc1, although acetone and ethyl acetate decreased the laccase activity. The presence of hexane (40%, v/v) caused a 58% increase in activity. Laccase KbLcc1 could be applied in the decolorization of synthetic dyes and in the biotransformation of ferulic acid to vanillin. After 5 days of reaction at 20 °C, pH 3.5, with 1 mM ABTS as a mediator, the vanillin concentration was 21.9 mg/L and the molar yield of transformation reached 14.39%.

scholarly journals Solid-state fermentation with orange waste: optimization of Laccase production from Pleurotus pulmonarius CCB-20 and decolorization of synthetic dyes

2020 ◽  
Vol 42 ◽  
pp. e52699 ◽  
Author(s):  
Alex Graça Contato ◽  
Fabíola Dorneles In´ácio ◽  
Tatiane Brugnari ◽  
Caroline Aparecida Vaz de Araújo ◽  
Giselle Maria Maciel ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Laccase Activity ◽  
Fermentation Medium ◽  
Laccase Production ◽  
Synthetic Dyes ◽  
Pleurotus Pulmonarius ◽  
Biotechnological Potential ◽  
Orange Waste ◽  
Oxidoreductase Enzymes

Laccases are oxidoreductase enzymes that have the ability to oxidize phenolic substrates. Its biotechnological potential has been greatly explored in many areas as biotechnology industry, bioremediation of dyes, food industry and environmental microbiology. The aim of this study was maximize the laccase production by Pleurotus pulmonarius (Fr.) Quélet in solid-state fermentation (SSF) using orange waste as substrate. After optimization the capability of the crude laccase to decolorize dyes was analyzed. The fermentation medium in the solid-state was optimized by applying a factorial design. After statistics optimization, laccase activity increased two times. The laccase activity appears to be correlated with the ability of crude extract to decolorize some industrial dyes. The optimized laccase was characterized with respect to optimum pH, influence of temperature and salts. Our results demonstrate that P. pulmonarius was an efficient producer of an important industrial enzyme, laccase, in a cheap solid-state system using orange waste as substrate.

scholarly journals Activity of Laccase Enzyme Present in the Phenol-Contaminated Sediments of the Marilao-Meycauayan-Obando River System, Philippines

2021 ◽  
Vol 37 (1) ◽  
pp. 162-168
Author(s):  
King Dave G. Martin ◽  
Maria Fatima T. Astrero ◽  
Laurence Anthony N. Mallari ◽  
Roland M. Hipol
Keyword(s):  
Sulfonic Acid ◽  
Laccase Activity ◽  
River System ◽  
Contaminated Sediments ◽  
Phenol Concentration ◽  
Sampling Area ◽  
The Family ◽  
Bacteria And Fungi ◽  
Laccase Enzyme ◽  
Collection Sites

Laccases are enzymes produced by different microbes like bacteria and fungi. These enzymes are members of the family of oxidases and are capable of oxidizing phenolics into non-toxic forms. Sediments were collected from the Marilao-Meycauayan-Obando River System, specifically from the sampling area connected to leather tanneries, which directly dump their effluents into the river. This study aimed to determine the presence of laccase activity of sediments of Meycauayan River where effluents of leather factories and tanneries are directly dumped. Concentration of the phenolic compounds from five collection sites were measured. Collected phenol - contaminated sediments were tested for laccase activity using ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)). Laccase was extracted from the collected sediments and used for the degradation of phenol. Reduction of phenol concentration by the extracted laccase reached 79.82% to as high as90.84%with a starting phenol concentration of 27.5 mmol per sample. Three strains of phenol-degrading ligninolytic bacteria closely related to Lysinibacillus xylanilyticus, Lysinibacillus fusiformisss, and Lysinibacillus sphaericuswere identified to be present in the river’s sediment which could produce laccase and facilitate degradation of phenol.

Decolorization of Alizarin Red and other synthetic dyes by a recombinant laccase from Pichia pastoris

2013 ◽  
Vol 36 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Miaomiao Zheng ◽  
Yujie Chi ◽  
Hongwei Yi ◽  
Shuli Shao
Keyword(s):  
Pichia Pastoris ◽  
Synthetic Dyes ◽  
Alizarin Red ◽  
Recombinant Laccase

scholarly journals SBA-15 with sulfonic acid groups as a Green Catalyst for the acetoxylation of α-pinene

2012 ◽  
Vol 163 ◽  
pp. 237-242 ◽  
Author(s):  
J. Machado ◽  
J.E. Castanheiro ◽  
I. Matos ◽  
A.M. Ramos ◽  
J. Vital ◽  
...  
Keyword(s):  
Sulfonic Acid ◽  
Green Catalyst ◽  
Sulfonic Acid Groups

scholarly journals Screening of Novel Laccase Producers—Isolation and Characterization of Cold-Adapted Laccase from Kabatiella bupleuri G3 Capable of Synthetic Dye Decolorization

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 828
Author(s):  
Katarzyna M. Wiśniewska ◽  
Aleksandra Twarda-Clapa ◽  
Aneta M. Białkowska
Keyword(s):  
Morphological Characteristics ◽  
Tween 80 ◽  
Dye Decolorization ◽  
Defined Medium ◽  
Industrial Applications ◽  
Maximum Activity ◽  
Its2 Region ◽  
Synthetic Dyes ◽  
Cold Adapted ◽  
Isolation And Characterization

Psychrophilic laccases catalyzing the bond formation in mild, environmentally friendly conditions are one of the biocatalysts at the focus of green chemistry. Screening of 41 cold-adapted strains of yeast and yeast-like fungi revealed a new laccase-producing strain, which was identified as Kabatiella bupleuri G3 IBMiP according to the morphological characteristics and analysis of sequences of the D1/D2 regions of 26S rDNA domain and the ITS1–5,8S–ITS2 region. The extracellular activity of laccase in reaction with 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) at the optimal pH 3.5 was 215 U/L after 15 days of growth in a medium with waste material and 126 U/L after 25 days of cultivation in a defined medium. Copper (II) ions (0.4 mM), Tween 80 (1.0 mM) and ascorbic acid (5.0 mM) increased the production of laccase. The optimum temperature for enzyme operation is in the range of 30–40 °C and retains over 60% of the maximum activity at 10 °C. New laccase shows high thermolability—half-life at 40 °C was only 60 min. Enzyme degradation of synthetic dyes was the highest for crystal violet, i.e., 48.6% after 1-h reaction with ABTS as a mediator. Outcomes of this study present the K. bupleuri laccase as a potential psychrozyme for environmental and industrial applications.

scholarly journals An Easy Method for Screening and Detection of Laccase Activity

2017 ◽  
Vol 11 (1) ◽  
pp. 89-93 ◽  
Author(s):  
Albino A. Dias ◽  
António J.S. Matos ◽  
Irene Fraga ◽  
Ana Sampaio ◽  
Rui M.F. Bezerra
Keyword(s):  
Sulfonic Acid ◽  
Laccase Activity ◽  
Direct Detection ◽  
Simultaneous Detection ◽  
Flat Bottom ◽  
Easy Method ◽  
Naked Eye ◽  
Microbial Cultures

Objective: An instrument-free assay was developed for simultaneous detection of laccase activity in a large number of samples as diverse as screening of laccase-producing microbial cultures or chromatographic fractions. Method: Dried paper discs previously impregnated with 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) were placed on a flat-bottom microplate (a simple way to avoid misidentification) and loaded with an aliquot from each sample. Results: Discs corresponding to samples containing laccase activity become green-bluish colored within first ten minutes of reaction, allowing direct detection through simple naked-eye inspection. Conclusion: As an example, this easy process was applied to the laccase purification in order to eliminate chromatographic fractions that did not contain laccase activity, thus reducing the number of spectrophotometric assays.

scholarly journals Production and synthetic dyes decolourization capacity of a recombinant laccase from Pichia pastoris

2009 ◽  
Vol 107 (4) ◽  
pp. 1149-1156 ◽  
Author(s):  
L. Lu ◽  
M. Zhao ◽  
S.-C. Liang ◽  
L.-Y. Zhao ◽  
D.-B. Li ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Synthetic Dyes ◽  
Recombinant Laccase

scholarly journals Biodecolorization of Anthraquinone and Azo Dyes by Newly Isolated Indonesian White-Rot Fungi

2021 ◽  
Vol 13 (1) ◽  
pp. 16-25
Author(s):  
Kharisma Panji Ramadhan ◽  
Sita Heris Anita ◽  
Maulida Oktaviani ◽  
Fahriya Puspita Sari ◽  
Raden Permana Budi Laksana ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Azo Dyes ◽  
Dna Analysis ◽  
Laccase Activity ◽  
Environmental Concern ◽  
White Rot Fungi ◽  
White Rot ◽  
Contaminated Water ◽  
Synthetic Dyes ◽  
Fungal Strains

Water pollution by dyes represents from dyestuff industry becomes an environmental concern. Finding new isolates capable of decolorizing these dyes is important. The study aimed to assess the new isolates of white-rot fungi (WRF) as decolorizing agent of anthraquinone and azo dyes. Decolorization assay were conducted in Agar plates and liquid medium. During the decolorization, laccase activities produced by the fungal strains were analyzed. Identification of the fungal strains were investigated using molecular DNA analysis. The results showed that isolates M3, H18, and GP1 were able to decolorize anthraquinone and azo dyes in Agar and liquid medium. Based on DNA analysis, isolates M3, H18, and GP1 have the similarity to Trametes sanguinea, Trametes polyzona, and Neofomitella guangxiensis, respectively. Among the fungi, T. polyzona H18 exhibited high decolorization ability (70–90%) to the dyes (100 mg/L) after 96-hours incubation. Laccase activity was fluctuated during the reactions with tendency to increase at the beginning until its peak, then decreased at the end of incubation. This study demonstrated the potential of the new isolates from Indonesia to decolorize anthraquinone and azo dyes. The results of the study can provide an alteranative for bioremediation agents of contaminated water by synthetic dyes.

scholarly journals Deep eutectic solvents (DESs) as powerful and recyclable catalysts and solvents for the synthesis of 3,4-dihydropyrimidin-2(1H)-ones/thiones

2019 ◽  
Vol 8 (1) ◽  
pp. 568-576 ◽  
Author(s):  
Yingna Cui ◽  
Changping Li ◽  
Ming Bao
Keyword(s):  
Catalytic Activity ◽  
Sulfonic Acid ◽  
Choline Chloride ◽  
Catalytic Efficiency ◽  
Aromatic Aldehydes ◽  
Deep Eutectic Solvents ◽  
Green Catalyst ◽  
Toluene Sulfonic Acid ◽  
Recyclable Catalysts ◽  
Main Factor

Abstract Deep eutectic solvents (DESs) are successfully used as powerful and recyclable catalysts and solvents for the synthesis of 3,4-dihydropyrimidin-2(1H)-ones/thiones (DHPMs). The acidity of DESs is the main factor that determines catalytic activity. DESs, based on p-toluene sulfonic acid (PTSA) and choline chloride (ChCl), exhibits the highest catalytic activity. ChCl/2PTSA is suitable for a vast variety of aromatic aldehydes with electron-donating and electron-withdrawing groups, different β-diketonates, and urea or thiourea to obtain the corresponding DHPMs. Furthermore, DESs can be recycled easily after synthesis. The reused DESs achieve catalytic efficiency six times without significant changes. This study will provide a new green catalyst and efficient process for the synthesis of DHPMs.

scholarly journals A novel superparamagnetic powerful guanidine-functionalized γ-Fe2O3 based sulfonic acid recyclable and efficient heterogeneous catalyst for microwave-assisted rapid synthesis of quinazolin-4(3H)-one derivatives in Green media

RSC Advances ◽  
2021 ◽  
Vol 11 (48) ◽  
pp. 29948-29959
Author(s):  
Fateme Haji Norouzi ◽  
Naser Foroughifar ◽  
Alireza Khajeh-Amiri ◽  
Hoda Pasdar
Keyword(s):  
Heterogeneous Catalyst ◽  
Sulfonic Acid ◽  
Condensation Reaction ◽  
Rapid Synthesis ◽  
One Pot ◽  
Green Catalyst ◽  
Microwave Assisted ◽  
One Pot Synthesis ◽  
The One ◽  
Efficient Heterogeneous Catalyst

A novel organic–inorganic nanohybrid superparamagnetic nanocatalyst (γ-Fe2O3@CPTMS–guanidine@SO3H) represents an efficient and green catalyst for the one-pot synthesis of quinazolin-4(3H)-one derivatives via a three-component condensation reaction.

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