scholarly journals Employing Extracellular Matrix-Based Tissue Engineering Strategies for Age-Dependent Tissue Degenerations

2021 ◽  
Vol 22 (17) ◽  
pp. 9367
Author(s):  
Yeonggwon Jo ◽  
Seung Hyeon Hwang ◽  
Jinah Jang
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
Stem Cell Maintenance ◽  
Engineered Tissues ◽  
Associated Symptoms ◽  
And Function ◽  
Regenerative Therapies ◽  
Cellular Components ◽  
Primary Risk Factor

Tissues and organs are not composed of solely cellular components; instead, they converge with an extracellular matrix (ECM). The composition and function of the ECM differ depending on tissue types. The ECM provides a microenvironment that is essential for cellular functionality and regulation. However, during aging, the ECM undergoes significant changes along with the cellular components. The ECM constituents are over- or down-expressed, degraded, and deformed in senescence cells. ECM aging contributes to tissue dysfunction and failure of stem cell maintenance. Aging is the primary risk factor for prevalent diseases, and ECM aging is directly or indirectly correlated to it. Hence, rejuvenation strategies are necessitated to treat various age-associated symptoms. Recent rejuvenation strategies focus on the ECM as the basic biomaterial for regenerative therapies, such as tissue engineering. Modified and decellularized ECMs can be used to substitute aged ECMs and cell niches for culturing engineered tissues. Various tissue engineering approaches, including three-dimensional bioprinting, enable cell delivery and the fabrication of transplantable engineered tissues by employing ECM-based biomaterials.

Functional Imaging of Matrix Structure of Cryopreserved Engineered Tissues Using Back-Directional Gated Mesoscopic Imaging

2012 ◽  
Author(s):  
Young L. Kim ◽  
Zhengbin Xu ◽  
Altug Ozcelikkale ◽  
Bumsoo Han
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
Clinical Use ◽  
Matrix Structure ◽  
Non Invasive ◽  
Engineered Tissues ◽  
Cell Tissue ◽  
Matrix Interactions ◽  
Non Destructive

Successful cryopreservation of engineered tissues (ETs) can greatly advance the access and availability of cell/tissue engineering products for clinical use. One of the key challenges in cryopreserving ETs is that the functionality of ETs should be maintained throughout the preservation process. Many of the functionalities are associated with the extracellular matrix (ECM) microstructure, which in turn can be a crucial marker for the post-thaw functionality. Recent studies also reported that the ECM microstructure can be affected by freezing-induced cell-fluid-matrix interactions.1–3 Thus, it is critical to assess three-dimensional (3-D) matrix structure of cryopreserved ETs in a non-destructive, non-invasive, and rapid manner.

The ECM-Integrin-Cytoskeletal Link: A Possible Site for Mechanical Signaling in the Heart

2000 ◽  
Vol 6 (S2) ◽  
pp. 542-543
Author(s):  
L. Terracio ◽  
W. Carver ◽  
R.L. Price ◽  
R. Salters ◽  
D.G. Simpson
Keyword(s):  
Extracellular Matrix ◽  
Cardiac Myocytes ◽  
Three Dimensional ◽  
Integrin Receptors ◽  
Normal Pattern ◽  
Mechanical Signaling ◽  
Dimensional Network ◽  
Heart Contraction ◽  
And Function ◽  
Cellular Components

The cardiac interstitium is a diverse system of extracellular-matrix (ECM) components organized into a stress tolerant three-dimensional network that interconnects the cellular components of the heart. During each contraction of the heart, a complex set of mechanical forces is transmitted through the cardiac interstitium and is applied to the cardiac myocytes and fibroblasts. In comparison to the relaxed heart, each contractile wave results in a rapid reorganization of the extracellular matrix and cell cytoskeleton. A number of studies have shown that by varying the normal pattern of applied mechanical force that occurs during heart contraction, both the phenotype and function of myocytes and fibroblasts in the heart may be altered.At the molecular level components of the ECM are attached to the cardiac myocytes and fibroblasts via specific transmembrane integrin receptors. Each integrin is comprised of an extracellular domain that attaches to an ECM component, and an intracellular domain that attaches to a cytoplasmic component of the cell.

scholarly journals Extracellular Matrix-Based Biomaterials for Cardiovascular Tissue Engineering

2021 ◽  
Vol 8 (11) ◽  
pp. 137
Author(s):  
Astha Khanna ◽  
Maedeh Zamani ◽  
Ngan F. Huang
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Regenerative Medicine ◽  
Three Dimensional ◽  
Vascular Differentiation ◽  
Cardiovascular Tissue ◽  
Cellular Behavior ◽  
Cardiovascular Tissue Engineering ◽  
And Function

Regenerative medicine and tissue engineering strategies have made remarkable progress in remodeling, replacing, and regenerating damaged cardiovascular tissues. The design of three-dimensional (3D) scaffolds with appropriate biochemical and mechanical characteristics is critical for engineering tissue-engineered replacements. The extracellular matrix (ECM) is a dynamic scaffolding structure characterized by tissue-specific biochemical, biophysical, and mechanical properties that modulates cellular behavior and activates highly regulated signaling pathways. In light of technological advancements, biomaterial-based scaffolds have been developed that better mimic physiological ECM properties, provide signaling cues that modulate cellular behavior, and form functional tissues and organs. In this review, we summarize the in vitro, pre-clinical, and clinical research models that have been employed in the design of ECM-based biomaterials for cardiovascular regenerative medicine. We highlight the research advancements in the incorporation of ECM components into biomaterial-based scaffolds, the engineering of increasingly complex structures using biofabrication and spatial patterning techniques, the regulation of ECMs on vascular differentiation and function, and the translation of ECM-based scaffolds for vascular graft applications. Finally, we discuss the challenges, future perspectives, and directions in the design of next-generation ECM-based biomaterials for cardiovascular tissue engineering and clinical translation.

scholarly journals Harnessing the Topography of 3D Spongy-Like Electrospun Bundled Fibrous Scaffold via a Sharply Inclined Array Collector

Polymers ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1444 ◽  
Author(s):  
Sun Hee Cho ◽  
Jeong In Kim ◽  
Cheol Sang Kim ◽  
Chan Hee Park ◽  
In Gi Kim
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
Living System ◽  
Pivotal Role ◽  
Skin Tissue ◽  
Target Tissue ◽  
Fibrous Scaffold ◽  
Electrospun Scaffolds ◽  
Directional Change

To date, many researchers have studied a considerable number of three-dimensional (3D) cotton-like electrospun scaffolds for tissue engineering, including the generation of bone, cartilage, and skin tissue. Although numerous 3D electrospun fibrous matrixes have been successfully developed, additional research is needed to produce 3D patterned and sophisticated structures. The development of 3D fibrous matrixes with patterned and sophisticated structures (FM-PSS) capable of mimicking the extracellular matrix (ECM) is important for advancing tissue engineering. Because modulating nano to microscale features of the 3D fibrous scaffold to control the ambient microenvironment of target tissue cells can play a pivotal role in inducing tissue morphogenesis after transplantation in a living system. To achieve this objective, the 3D FM-PSSs were successfully generated by the electrospinning using a directional change of the sharply inclined array collector. The 3D FM-PSSs overcome the current limitations of conventional electrospun cotton-type 3D matrixes of random fibers.

scholarly journals The Effects of Ionising and Non-Ionising Electromagnetic Radiation on Extracellular Matrix Proteins

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 3041
Author(s):  
Ren Jie Tuieng ◽  
Sarah H. Cartmell ◽  
Cliona C. Kirwan ◽  
Michael J. Sherratt
Keyword(s):  
Extracellular Matrix ◽  
Electromagnetic Radiation ◽  
Cell Biology ◽  
Biological Effects ◽  
Well Being ◽  
Ecm Proteins ◽  
Clinical Consequences ◽  
Radiation Induced ◽  
And Function ◽  
Cellular Components

Exposure to sub-lethal doses of ionising and non-ionising electromagnetic radiation can impact human health and well-being as a consequence of, for example, the side effects of radiotherapy (therapeutic X-ray exposure) and accelerated skin ageing (chronic exposure to ultraviolet radiation: UVR). Whilst attention has focused primarily on the interaction of electromagnetic radiation with cells and cellular components, radiation-induced damage to long-lived extracellular matrix (ECM) proteins has the potential to profoundly affect tissue structure, composition and function. This review focuses on the current understanding of the biological effects of ionising and non-ionising radiation on the ECM of breast stroma and skin dermis, respectively. Although there is some experimental evidence for radiation-induced damage to ECM proteins, compared with the well-characterised impact of radiation exposure on cell biology, the structural, functional, and ultimately clinical consequences of ECM irradiation remain poorly defined.

scholarly journals Fabrication of 3D Printing Scaffold with Porcine Skin Decellularized Bio-Ink for Soft Tissue Engineering

Materials ◽  
2020 ◽  
Vol 13 (16) ◽  
pp. 3522
Author(s):  
Su Jeong Lee ◽  
Jun Hee Lee ◽  
Jisun Park ◽  
Wan Doo Kim ◽  
Su A Park
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
3D Printing ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Research Groups ◽  
Porcine Skin ◽  
Alginate Hydrogel ◽  
Chemical Treatments ◽  
Soft Tissue Engineering

Recently, many research groups have investigated three-dimensional (3D) bioprinting techniques for tissue engineering and regenerative medicine. The bio-ink used in 3D bioprinting is typically a combination of synthetic and natural materials. In this study, we prepared bio-ink containing porcine skin powder (PSP) to determine rheological properties, biocompatibility, and extracellular matrix (ECM) formation in cells in PSP-ink after 3D printing. PSP was extracted without cells by mechanical, enzymatic, and chemical treatments of porcine dermis tissue. Our developed PSP-containing bio-ink showed enhanced printability and biocompatibility. To identify whether the bio-ink was printable, the viscosity of bio-ink and alginate hydrogel was analyzed with different concentration of PSP. As the PSP concentration increased, viscosity also increased. To assess the biocompatibility of the PSP-containing bio-ink, cells mixed with bio-ink printed structures were measured using a live/dead assay and WST-1 assay. Nearly no dead cells were observed in the structure containing 10 mg/mL PSP-ink, indicating that the amounts of PSP-ink used were nontoxic. In conclusion, the proposed skin dermis decellularized bio-ink is a candidate for 3D bioprinting.

scholarly journals Electrospun nanofibers for the fabrication of engineered vascular grafts

2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Sonia Fathi Karkan ◽  
Soodabeh Davaran ◽  
Reza Rahbarghazi ◽  
Roya Salehi ◽  
Abolfazl Akbarzadeh
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Physicochemical Properties ◽  
Review Paper ◽  
Vascular Grafts ◽  
Three Dimensional ◽  
Electrospun Nanofibers ◽  
Electrospun Fibers

Abstract Attention has recently increased in the application of electrospun fibers because of their putative capability to create nanoscale platforms toward tissue engineering. To some extent, electrospun fibers are applicable to the extracellular matrix by providing a three-dimensional microenvironment in which cells could easily acquire definite functional shape and maintain the cell-to-cell connection. It is noteworthy to declare that placement in different electrospun substrates with appropriate physicochemical properties enables cells to promote their bioactivities, dynamics growth and differentiation, leading to suitable restorative effects. This review paper aims to highlight the application of biomaterials in engineered vascular grafts by using electrospun nanofibers to promote angiogenesis and neovascularization

scholarly journals Fabrication of Nanohydroxyapatite/Poly(caprolactone) Composite Microfibers Using Electrospinning Technique for Tissue Engineering Applications

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Mohd Izzat Hassan ◽  
Tao Sun ◽  
Naznin Sultana
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
Average Diameter ◽  
Tissue Engineering Scaffold ◽  
Engineering Applications ◽  
Electrospinning Technique ◽  
Fibrous Scaffolds ◽  
Nanosized Hydroxyapatite ◽  
Poly Caprolactone

Tissue engineering fibrous scaffolds serve as three-dimensional (3D) environmental framework by mimicking the extracellular matrix (ECM) for cells to grow. Biodegradable polycaprolactone (PCL) microfibers were fabricated to mimic the ECM as a scaffold with 7.5% (w/v) and 12.5% (w/v) concentrations. Lower PCL concentration of 7.5% (w/v) resulted in microfibers with bead defects. The average diameter of fibers increased at higher voltage and the distance of tip to collector. Further investigation was performed by the incorporation of nanosized hydroxyapatite (nHA) into microfibers. The incorporation of 10% (w/w) nHA with 7.5% (w/v) PCL solution produced submicron sized beadless fibers. The microfibrous scaffolds were evaluated using various techniques. Biodegradable PCL and nHA/PCL could be promising for tissue engineering scaffold application.

scholarly journals Of balls, inks and cages: Hybrid biofabrication of 3D tissue analogs

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Nicanor Moldovan ◽  
Leni Maldovan ◽  
Michael Raghunath
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Hybrid Approach ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Support Structures ◽  
Cell Clusters ◽  
3D Space ◽  
Technical Solutions

The overarching principle of three-dimensional (3D) bioprinting is the placing of cells or cell clusters in the 3D space to generate a cohesive tissue microarchitecture that comes close to in vivo characteristics. To achieve this goal, several technical solutions are available, generating considerable combinatorial bandwidth: (i) Support structures are generated first, and cells are seeded subsequently; (ii) alternatively, cells are delivered in a printing medium, so-called “bioink,” that contains them during the printing process and ensures shape fidelity of the generated structure; and (iii) a “scaffold-free” version of bioprinting, where only cells are used and the extracellular matrix is produced by the cells themselves, also recently entered a phase of accelerated development and successful applications. However, the scaffold-free approaches may still benefit from secondary incorporation of scaffolding materials, thus expanding their versatility. Reversibly, the bioink-based bioprinting could also be improved by adopting some of the principles and practices of scaffold-free biofabrication. Collectively, we anticipate that combinations of these complementary methods in a “hybrid” approach, rather than their development in separate technological niches, will largely increase their efficiency and applicability in tissue engineering.

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