scholarly journals Lignin/Carbohydrate Complex Isolated from Posidonia oceanica Sea Balls (Egagropili): Characterization and Antioxidant Reinforcement of Protein-Based Films

2021 ◽  
Vol 22 (17) ◽  
pp. 9147 ◽  
Author(s):  
Seyedeh Fatemeh Mirpoor ◽  
Odile Francesca Restaino ◽  
Chiara Schiraldi ◽  
Concetta Valeria L. Giosafatto ◽  
Francesco Ruffo ◽  
...  
Keyword(s):  
High Performance ◽  
Glucuronic Acid ◽  
Dry Weight ◽  
Posidonia Oceanica ◽  
Water Soluble ◽  
Size Exclusion ◽  
Polymeric Chain ◽  
Antioxidant Additive ◽  
Chromatography Analysis ◽  
Carbohydrate Complex

A lignin fraction (LF) was extracted from the sea balls of Posidonia oceanica (egagropili) and extensively dialyzed and characterized by FT-IR and NMR analyses. LF resulted water soluble and exhibited a brownish-to-black color with the highest absorbance in the range of 250–400 nm, attributed to the chromophore functional groups present in the phenylpropane-based polymer. LF high-performance size exclusion chromatography analysis showed a highly represented (98.77%) species of 34.75 kDa molecular weight with a polydispersity index of 1.10 and an intrinsic viscosity of 0.15. Quantitative analysis of carbohydrates indicated that they represented 28.3% of the dry weight of the untreated egagropili fibers and 72.5% of that of LF. In particular, eight different monosaccharides were detected (fucose, arabinose, rhamnose, galactose, glucose, xylose, glucosamine and glucuronic acid), glucuronic acid (46.6%) and rhamnose (29.6%) being the most present monosaccharides in the LF. Almost all the phenol content of LF (113.85 ± 5.87 mg gallic acid eq/g of extract) was water soluble, whereas around 22% of it consisted of flavonoids and only 10% of the flavonoids consisted of anthocyanins. Therefore, LF isolated from egagropili lignocellulosic material could be defined as a water-soluble lignin/carbohydrate complex (LCC) formed by a phenol polymeric chain covalently bound to hemicellulose fragments. LCC exhibited a remarkable antioxidant activity that remained quite stable during 6 months and could be easily incorporated into a protein-based film and released from the latter overtime. These findings suggest egagropili LCC as a suitable candidate as an antioxidant additive for the reinforcement of packaging of foods with high susceptibility to be deteriorated in aerobic conditions.

scholarly journals Chromatographic Length Separation and Photoluminescence Study on DNA-Wrapped Single-Wall and Double-Wall Carbon Nanotubes

2009 ◽  
Vol 2009 ◽  
pp. 1-8 ◽  
Author(s):  
Yuki Asada ◽  
Toshiki Sugai ◽  
Ryo Kitaura ◽  
Hisanori Shinohara
Keyword(s):  
Carbon Nanotubes ◽  
Raman Spectroscopy ◽  
High Performance ◽  
Water Soluble ◽  
Size Exclusion ◽  
Photoluminescence Study ◽  
Force Microscopy ◽  
Atomic Force ◽  
Length Separation ◽  
Double Wall

Water-soluble DNA-wrapped single-wall and double-wall carbon nanotubes (DNA-SWNTs, DNA-DWNTs) have been well separated by length incorporating size-exclusion high-performance liquid chromatography (HPLC). The morphology and electronic properties of the size- (length-) separated DNA-SWNTs and -DWNTs are investigated by atomic force microscopy (AFM), photoluminescence (PL), and Raman spectroscopy. By using length-separated DNA-SWNTs and -DWNTs, we have found that PL intensity of the DNA-SWNTs varies sensitively depending not only on the chirality (or diameter) but more importantly on the length of the hybrids.

Quantification of galantamine in Narcissus tazetta and Galanthus nivalis (Amaryllidaceae) populations growing wild in Iran

2017 ◽  
Vol 16 (2) ◽  
pp. 188-192 ◽  
Author(s):  
Majid Rahimi Khonakdari ◽  
Mohammad Hossein Mirjalili ◽  
Abbas Gholipour ◽  
Hassan Rezadoost ◽  
Mahdi Moridi Farimani
Keyword(s):  
High Performance ◽  
Large Scale ◽  
Dry Weight ◽  
Narcissus Tazetta ◽  
Suitable Material ◽  
Chromatography Analysis ◽  
Enhanced Production ◽  
Galanthus Nivalis ◽  
Alternative Sources ◽  
Plant Sources

AbstractGalantamine (GAL), a morphine-like alkaloid produced by some members of the Amaryllidaceae plant family, is a possible therapeutic agent in Alzheimer's disease because of its central cholinergic effects. GAL has been extracted from the plant sources or produced synthetically for pharmaceutical use. Limited supply of the natural source and high cost of synthetic production has led to a search for alternative sources of this valuable compound. In the present study, a total of six Galanthus nivalis populations (GNPs) and 11 Narcissus tazetta populations (NTPs) were collected across different regions of Iran and were then subjected to the high-performance liquid chromatography analysis for their GAL quantification. The GAL content ranged from 0.05 to 0.36 mg/g dry weight (DW) in the bulbs of GNPs, and from 0.03 to 0.33 mg/g DW in the bulbs of NTPs. Maximum content of GAL (0.36 and 0.33 mg/g DW) was measured in the Zirab population of G. nivalis and Ghaemshahr population of N. tazetta, respectively. Our results provided a suitable material for further agronomical and biotechnological strategies for enhanced production of valuable GAL compound on a large scale.

scholarly journals Structure and Bioactivity Screening of a Low Molecular Weight Ulvan from the Green Alga Ulothrix flacca

Marine Drugs ◽  
2018 ◽  
Vol 16 (8) ◽  
pp. 281 ◽  
Author(s):  
Peipei Li ◽  
Songsong Wen ◽  
Kunlai Sun ◽  
Yuqin Zhao ◽  
Yin Chen
Keyword(s):  
Molecular Weight ◽  
Green Algae ◽  
Glucuronic Acid ◽  
Water Soluble ◽  
Size Exclusion ◽  
Low Molecular Weight ◽  
Immunomodulating Activity ◽  
Sulfate Content ◽  
Exclusion Chromatography

A water-soluble low molecular–weight polysaccharide named UP2-1 was isolated and purified from the marine green algae Ulothrix flacca using ion-exchange and size-exclusion chromatography. Composition and characteristics analyses showed that UP2-1 was a sulfated glucuronorhamnan consisting of rhamnose and glucuronic acid in a ratio of 2:1 with 21% sulfate content and a molecular weight of 5.0 kDa. Structural properties were determined using desulfation and methylation analyses combined with infrared spectrum (IR), gas chromatography-mass spectrometer (GC-MS) and nuclear magnetic resonance (NMR). The results showed that UP2-1 was a type of ulvan composed of alternate 4-linked-α-L-rhamnose residues (→4)-α-L-Rha(1→) and 4-linked-β-D-glucouronoc acid residues. The sulfate groups were mainly present in the O-3 position of →4)-α-L-Rha(1→. Most (70%) of the rhamnose was sulfated. UP2-1 also had a small amount of →4)-α-L-Rha(1→ branch at the O-2 position of the →4)-α-L-Rha(1→. UP2-1 exhibited significant anticoagulant and immunomodulating activity in vitro. This study demonstrated that the green algae Ulothrix flacca, which is used as a food and traditional marine herb in China, could also be considered as a source of bioactive ulvan.

scholarly journals Synthesis and Accumulation of Cyanophycin in Transgenic Strains of Saccharomyces cerevisiae

2008 ◽  
Vol 74 (11) ◽  
pp. 3410-3418 ◽  
Author(s):  
Anna Steinle ◽  
Fred Bernd Oppermann-Sanio ◽  
Rudolf Reichelt ◽  
Alexander Steinbüchel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspartic Acid ◽  
High Performance ◽  
Sodium Dodecyl ◽  
Copper Ion ◽  
Inducible Promoter ◽  
Water Soluble ◽  
Minor Amount ◽  
Chromatography Analysis ◽  
Vector Systems

ABSTRACT Cyanophycin [multi-l-arginyl-poly(l-aspartic acid) (CGP)] was, for the first time, produced in yeast. As yeasts are very important production organisms in biotechnology, it was determined if CGP can be produced in two different strains of Saccharomyces cerevisiae. The episomal vector systems pESC (with the galactose-inducible promoter GAL1) and pYEX-BX (with the copper ion-inducible promoter CUP1) were chosen to express the cyanophycin synthetase gene from the cyanobacterium Synechocystis sp. strain PCC 6308 (cphA 6308) in yeast. Expression experiments with transgenic yeasts revealed that the use of the CUP1 promoter is much more efficient for CGP production than the GAL1 promoter. As observed by electrophoresis of isolated CGP in sodium dodecyl sulfate-polyacrylamide gels, the yeast strains produced two different types of polymer: the water-soluble and the water-insoluble CGP were observed as major and minor forms of the polymer, respectively. A maximum CGP content of 6.9% (wt/wt) was detected in the cells. High-performance liquid chromatography analysis showed that the isolated polymers consisted mainly of the two amino acids aspartic acid and arginine and that, in addition, a minor amount (2 mol%) of lysine was present. Growth of transgenic yeasts in the presence of 15 mM lysine resulted in an incorporation of up to 10 mol% of lysine into CGP. Anti-CGP antibodies generated against CGP isolated from Escherichia coli TOP10 harboring cphA 6308 reacted with insoluble CGP but not with soluble CGP, if applied in Western or dot blots.

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