scholarly journals Potential Antioxidant and Anti-Inflammatory Function of Gynura procumbens Polyphenols Ligand

2021 ◽  
Vol 22 (16) ◽  
pp. 8716
Author(s):  
Hun Hwan Kim ◽  
Sang Eun Ha ◽  
Preethi Vetrivel ◽  
Pritam Bhagwan Bhosale ◽  
Seong Min Kim ◽  
...  
Keyword(s):  
Binding Affinity ◽  
High Performance ◽  
Kynurenic Acid ◽  
Screening Methods ◽  
Bioactive Ingredients ◽  
Pharmaceutical Industries ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Inflammatory Effect

The antioxidant and anti-inflammatory potentials of polyphenols contained in Gynura procumbens (GP) extract were systematically analyzed. Polyphenols in GP were analyzed for nine peaks using high-performance liquid chromatography (HPLC) combined with mass spectrometry (MS), and quantitatively determined through each standard. A total of nine polyphenolic compounds were identified in the samples and their MS data were tabulated. To determine the potential of bioactive ingredients targeting DPPH and COX-2, we analyzed them by ultrafiltration combined with LC. The results identified the major compounds exhibiting binding affinity for DPPH and COX-2. Caffeic acid, kynurenic acid, and chlorogenic acid showed excellent binding affinity to DPPH and COX-2, suggesting that they can be considered as major active compounds. Additionally, the anti-inflammatory effect of GP was confirmed in vitro. This study will not only be used to provide basic data for the application of GP to the food and pharmaceutical industries, but will also provide information on effective screening methods for other medicinal plants.

scholarly journals Anti- inflammatory effect of Prunus tomentosa Thunb total flavones in LPS-induced RAW264.7 cells

2019 ◽  
Vol 17 (1) ◽  
pp. 685-693
Author(s):  
Chen Xi ◽  
Liu Yuanyuan ◽  
Zhao Dongshuang ◽  
Fan Ziwei ◽  
Cao Shuang ◽  
...  
Keyword(s):  
Significant Inhibition ◽  
Raw264.7 Cells ◽  
Supernatant Fraction ◽  
Treatment Of Diabetes ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
The Impact ◽  
Cox 1 ◽  
Inflammatory Effect

AbstractIn this research, we investigated possible anti-inflammatory roles of Prunus tomentosa Thunb Total Flavones (PTTTF) in LPS-induced RAW264.7 cells. PTTTF (4μg/ml and 40μg/ml) was applied to RAW264.7 cells induced with 1μg/ml LPS to test the impact of these flavones on neutrophil phagocytosis in vitro. Levels of prostaglandin E2 (PGE2) and two pro-inflammatory interleukin cytokines (i.e. IL-6 and IL-1β) in the supernatant fraction were tested via Enzyme-linked immunosorbent assays (ELISA). Expression of cyclooxygenases COX-1 and COX-2 was detected via RT-PCR. Superoxide dismutase (SOD) content was determined with a spectrophotometric assay (Micromethod). The results revealed that PTTTF at doses higher than 4μg/ml reduces the content of IL-6, IL-1β and PGE2 (P < 0.05), and elevates the activity of SOD in LPS-induced RAW264.7 cells significantly (P < 0.05). PTTTF at 40μg/ml showed no significant effect on the expression of COX-1(P>0.05) but resulted in a significant inhibition of COX-2 in LPS-induced RAW264.7 cells (P<0.05). In summary, PTTTF had a substantial potential anti-inflammatory effect through the alteration of the synthesis of some cytokines and other mediators of the process of inflammation. Novelty statement - Prunus tomentosa Thunb Total Flavones (PTTTF) have known roles in the treatment of diabetes, but here we show that they are also potential anti-inflammatory agents. Our results show that PTTTF exhibited anti-inflammatory effects through altering the synthesis of some cytokines and other mediators of the inflammatory process.

scholarly journals In vitro Assessment of Anti-Inflammatory and COX-2 inhibitory action of some medicinal plants

2020 ◽  
Vol 93 (1) ◽  
Author(s):  
Usama Ramadhan
Keyword(s):  
Inhibitory Action ◽  
High Efficacy ◽  
Membrane Stabilization ◽  
Plant Constituents ◽  
In Vitro Assessment ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Inhibition Potency ◽  
Inflammatory Effect

Objective of this research was to evaluate the anti-inflammatory and COX-2 inhibitory properties of celery, myrrh, aqueous and alcoholic fenugreek plant extracts. The Human Red Blood Cell membrane stabilization (HRBC) method was used to evaluate the anti-inflammatory effect and the Enzyme Immunoassay (EIA) was used to evaluate the COX-2 inhibitory actions. The celery extract showed excellent anti-inflammatory and COX-2 inhibition potency. The other extracts (myrrh and fenugreek) showed less activity that might indicate high efficacy of celery. This may be attributed to the plant constituents, mainly to a compound known as 3-n-butylphthalide, which is the most powerful of all healing factors of celery.

scholarly journals Study on the Bioassay of Anti-Inflammatory Effects of Fuke Qianjin Capsule Based on COX-2 Inhibiting Activity

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Run-ran Ma ◽  
Xiao-juan Yang ◽  
Ying Huang ◽  
Shuai-shuai Chen ◽  
Xiao-he Xiao ◽  
...  
Keyword(s):  
Quality Control ◽  
High Performance ◽  
Linear Regression Analysis ◽  
Parallel Line ◽  
Multiple Linear Regression Analysis ◽  
Positive Control ◽  
Bioassay Method ◽  
Cox 2 ◽  
Anti Inflammatory

Fuke Qianjin Capsule (FKQJ) is a common TCM compound formula in the treatment of gynecological inflammation-related diseases. This study intends to explore and establish a bioassay method to further improve its quality control. The bioassay method for the determination of anti-inflammatory biopotency was established based on its inhibitory activity on recombinant human cyclooxygenase-2 (COX-2), an active target of FKQJ in the treatment of female pelvic inflammatory disease. We firstly established chemical fingerprint of 20 batches of FKQJ by ultra-high-performance liquid chromatography to identify the components and analyze the chemical similarities. The similarity within different batches of FKQJ was relatively high. The values of similarity of the 19 batches were between 0.973 and 0.995, while one batch’s similarity value was 0.813. Celecoxib, a selective inhibitor of COX-2, was chosen as the positive control drug in COX-2 activity assay to establish an anti-inflammatory biopotency detection method based on parallel line test of qualitative response. The methodological investigation showed that the method possessed good repeatability and precision. Secondly, the anti-inflammatory biopotency of 20 batches of FKQJ for inhibiting COX-2 was determined. The results showed that the biopotency of different batches of FKQJ ranged from 676 U/μg to 1310 U/μg, with average value of 918 U/μg and RSD of 16.7%. Based on multiple linear regression analysis, we found that three contents were highly correlated with the anti-inflammatory biopotency, while chlorogenic acid was validated of the strongest anti-inflammatory activity in vitro. Compared with chemical detection, bioassay can better reflect the quality fluctuation of different batches of products and correlate the known pharmacodynamic targets. The supplement of the bioassay method based on chemical evaluation is helpful to improve the quality control ability of Chinese patent medicine and ensure its clinical efficacy is stable and controllable.

scholarly journals Polymethoxyflavone Apigenin-Trimethylether Suppresses LPS-Induced Inflammatory Response in Nontransformed Porcine Intestinal Cell Line IPEC-J2

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Orsolya Farkas ◽  
Orsolya Palócz ◽  
Erzsébet Pászti-Gere ◽  
Péter Gálfi
Keyword(s):  
Gene Expression ◽  
Inflammatory Response ◽  
Intestinal Inflammation ◽  
Mrna Level ◽  
Intestinal Cell ◽  
Amplex Red ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Thein vitroanti-inflammatory effect of apigenin and its trimethylated analogue (apigenin-trimethylether) has been investigated in order to evaluate whether these flavonoids could attenuate LPS-induced inflammation in IPEC-J2 non-transformed intestinal epithelial cells. Levels of IL-6, IL-8, TNF-α, and COX-2 mRNA were measured as a marker of inflammatory response. The extracellular H2O2level in IPEC-J2 cells was also monitored by Amplex Red assay. Our data revealed that both compounds had significant lowering effect on the inflammatory response. Apigenin (at 25 μM) significantly decreased gene expression of IL-6 in LPS-treated cells, while apigenin-trimethylether in the same concentration did not influence IL-6 mRNA level. Both apigenin and apigenin-trimethylether reduced IL-8 gene expression significantly. TNF-αmRNA level was decreased by apigenin-trimethylether, which was not influenced by apigenin. Treatment with both flavonoids caused significant reduction in the mRNA level of COX-2, but the anti-inflammatory effect of the methylated analogue was more effective than the unmethylated one. Furthermore, both flavonoids reduced significantly the level of extracellular H2O2compared to the control cells. In conclusion, the methylated apigenin analogue could avoid LPS-induced intestinal inflammation and it could be applied in the future as an effective anti-inflammatory compound.

Anti-inflammatory effect of an Ayurveda polyherbal topical application and exploration of its mechanism of action

2017 ◽  
Vol 2 (06) ◽  
Author(s):  
Sitaram Ahalya ◽  
B. A. Venkatesh ◽  
R. Vijayasarathi ◽  
Tirumalapura Vijayanna Shalini
Keyword(s):  
Topical Application ◽  
Inflammatory Activity ◽  
Polyherbal Formulation ◽  
Tnf Α ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Ear Oedema ◽  
Inflammatory Effect

Background: Topical application of herbs is one of the recommended treatment modalities for Osteoarthritis (OA) in Ayurveda (Indian system of medicine). The current study intended to evaluate the anti-inflammatory effect of a polyherbal powder used as Upanaha (poultice) for OA by in vitro and in vivo techniques. Materials and Methods: The polyherbal formulation Upanaha Choornam (UC) was sourced from Vaidyaratnam Oushadhasala (P) Ltd., Thrissur, India. Changes in the secretion of TNF-α and NO and expression of Cox-2 genes were evaluated by semi quantitative PCR activity to establish anti-inflammatory action in vitro. Macrophages and connective tissue of mice were used as media for the former two experiments and only macrophages for the latter. In vivo anti–inflammatory activity was evaluated by TPA induced ear oedema in Swiss Albino mice (n=24), divided into 4 groups as Group I - saline treatment, Group II - Indomethacin treatment, and Groups III and IV treated with 30% and 60% of UC respectively. Results: In the in vitro study, UC at 1000 µg/ml and 500 µg/ml upregulated the COX-2 level by 0.08 and 0.03 folds respectively as compared to control. Release of TNF-α, and NO in LPS-induced RAW cells were significantly inhibited in a dose dependent manner. The TPA induced ear oedema significantly reduced in Groups III and IV (F=1250, p less than 0.001) Conclusion: The current study demonstrates the safety and anti-inflammatory activity of a polyherbal formulation Upanaha Choornam as a topical application. This indicates the potential of select herbs in managing degenerative conditions like OA.

scholarly journals Phytochemical Analysis, Anti-inflammatory, and Antioxidant Activities of Dendropanax dentiger Roots

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Li Yang ◽  
Yiwei Fang ◽  
Ronghua Liu ◽  
Junwei He
Keyword(s):  
High Performance ◽  
Cyclic Peptide ◽  
Antioxidant Activities ◽  
Inflammatory Diseases ◽  
Phytochemical Analysis ◽  
Chemical Compositions ◽  
Biological Functions ◽  
Cox 2 ◽  
Anti Inflammatory

Dendropanax dentiger root is a traditional medicinal plant in China and used to treat inflammatory diseases for centuries, but its phytochemical profiling and biological functions are still unknown. Thus, a rapid, efficient, and precise method based on ultra high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was applied to rapidly analyse the phytochemical profiling of D. dentiger with anti-inflammatory and antioxidant activities in vitro. As a result, a total of 78 chemical compositions, including 15 phenylpropanoids, 15 alkaloids, 14 flavonoids, 14 fatty acids, 7 phenols, 4 steroids, 4 cyclic peptides, 3 terpenoids, and 2 others, were identified or tentatively characterized in the roots of D. dentiger. Moreover, alkaloid and cyclic peptide were reported from D. dentiger for the first time. In addition, the ethanol crude extract of D. dentiger roots exhibited remarkable anti-inflammatory activity against cyclooxygenase- (COX-) 2 inhibitory and antioxidant activities in vitro. This study is the first to explore the phytochemical analysis and COX-2 inhibitory activity of D. dentiger. This study can provide important phytochemical profiles and biological functions for the application of D. dentiger roots as a new source of natural COX-2 inhibitors and antioxidants in pharmaceutical industry.

scholarly journals In vitro Inhibitory Activities of Lauraceae Aporphine Alkaloids

2010 ◽  
Vol 5 (3) ◽  
pp. 1934578X1000500
Author(s):  
Ericsson David Coy Barrera ◽  
Luis Enrique Cuca Suárez
Keyword(s):  
Platelet Aggregation ◽  
Inhibitory Activities ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Lox Inhibitors ◽  
Cox 1 ◽  
Inflammatory Effect

The in vitro anti-inflammatory effect of eight aporphine alkaloids isolated from the leaves of two Lauraceae plants (Pleurothyrium cinereum and Ocotea macrophylla) was evaluated through inhibition of two isozymes of cyclooxygenase (COX-1 and COX-2), 5-lipoxygenase (5-LOX), and platelet aggregation induced by PAF, AA and ADP. All alkaloids exhibited inhibitory activities against COX-2 (IC50 25.9-116 μM range) and PAF- and AA-induced platelet aggregation, while only four and three of them were good COX-1 and 5-LOX inhibitors, respectively. (+)-N-acetyl-nornantenine 6 was the most potent COX-2, 5-LOX, AA and PAF inhibitor.

Esculentic acid, a novel and selective COX-2 inhibitor with anti-inflammatory effect in vivo and in vitro

2014 ◽  
Vol 740 ◽  
pp. 532-538 ◽  
Author(s):  
Xiaofeng Niu ◽  
Qingli Mu ◽  
Weifeng Li ◽  
Huan Yao ◽  
Huani Li ◽  
...  
Keyword(s):  
Cox 2 ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Exogenous Vitamins K Exert Anti-Inflammatory Effects Dissociated from Their Role as Substrates for Synthesis of Endogenous MK-4 in Murine Macrophages Cell Line

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1571
Author(s):  
Anna Kieronska-Rudek ◽  
Agnieszka Kij ◽  
Patrycja Kaczara ◽  
Anna Tworzydlo ◽  
Marek Napiorkowski ◽  
...  
Keyword(s):  
Vitamin K ◽  
Wide Scope ◽  
Murine Macrophages ◽  
Endogenous Production ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
First Time ◽  
Inflammatory Action ◽  
Inflammatory Effect

Vitamins K exert a range of activities that extend far beyond coagulation and include anti-inflammatory effects, but the mechanisms involved in anti-inflammatory action remain unclear. In the present study, we showed that various forms of exogenous vitamins—K1, K3, K2 (MK-4, MK-5, MK-6 and MK-7)—regulated a wide scope of inflammatory pathways in murine macrophages in vitro, including NOS-2, COX-2, cytokines and MMPs. Moreover, we demonstrated for the first time that macrophages are able to synthesise endogenous MK-4 on their own. Vitamins with shorter isoprenoid chains—K1, K3 and MK-5—exhibited stronger anti-inflammatory potential than vitamins with longer isoprenoid chains (MK-6 and MK-7) and simultaneously were preferably used as a substrate for MK-4 endogenous production. Most interesting, atorvastatin pretreatment inhibited endogenous MK-4 production but had no impact on the anti-inflammatory activity of vitamin K. In summary, our results demonstrate that macrophages are able to synthesise endogenous MK-4 using exogenous vitamin K, and statin inhibits this process. However, the anti-inflammatory effect of exogenous vitamin K was independent of endogenous MK-4 synthesis.

scholarly journals Regenerative and anti-inflammatory effect of a novel bentonite complex on burn wounds

2021 ◽  
Author(s):  
Ju Young Lee ◽  
Han Na Suh ◽  
Kwan Young Choi ◽  
Chang Woo Song ◽  
Jeong Ho Hwang
Keyword(s):  
Cell Proliferation ◽  
Therapeutic Effect ◽  
Antioxidant Properties ◽  
Bentonite Clay ◽  
Skin Regeneration ◽  
Burn Wounds ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Abstract Background Bentonite, a montmorillonite clay, has been used as a classical remedy strategy for a long time. Recently, bentonite has been used as a raw material in cosmetic products because of its antibacterial and antioxidant properties. However, the therapeutic effect of bentonite on burn injuries has not yet been identified. Here, we explored the therapeutic effect of a novel bentonite complex, which was developed for medical use, on burn wounds and the anti-inflammatory function of bentonite clay in the complex in vitro. Methods A novel bentonite complex and bentonite clay were provided by C&L Biotech Co., Ltd. (Seoul, Korea). The elements of bentonite clay were analysed using inductively coupled plasma optical emission spectroscopy. Burn wounds were induced on the dorsal skin of two Yucatan minipigs, and tissue regeneration was determined by cell proliferation, angiogenesis, and collagen deposition using immunohistochemistry and Masson’s trichrome staining. Anti-inflammatory function was determined using quantitative real-time PCR and enzyme-linked immunosorbent assays. Results We found that by inducing collagen synthesis, cell proliferation, and angiogenesis, the bentonite complex improved skin regeneration in burn wounds. It was also identified that the expression of inflammatory cytokines associated with cyclooxygenase 2 (COX-2) signalling was significantly inhibited by treatment of the burn wounds with the bentonite complex. An in vitro study to identify the anti-inflammatory effect of bentonite clay, a major component of the bentonite complex, showed that COX-2 signalling was significantly regulated in both HacaT keratinocytes and 3D4/2 macrophage cell lines in vitro. Conclusions This study identified the therapeutic effect of a novel bentonite complex in burn wounds by inducing skin regeneration and bentonite-mediated anti-inflammatory responses with bentonite complex treatment.

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