scholarly journals Cold Atmospheric Plasma Changes the Amino Acid Composition of Solutions and Influences the Anti-Tumor Effect on Melanoma Cells

2021 ◽  
Vol 22 (15) ◽  
pp. 7886
Author(s):  
Stephanie Arndt ◽  
Fadi Fadil ◽  
Katja Dettmer ◽  
Petra Unger ◽  
Marko Boskovic ◽  
...  
Keyword(s):  
Amino Acids ◽  
Melanoma Cells ◽  
Atmospheric Plasma ◽  
Strong Impact ◽  
Active Components ◽  
Cellular Mechanisms ◽  
Cold Atmospheric Plasma ◽  
Decisive Importance ◽  
Other Substances ◽  
Dose Dependent

Cold Atmospheric Plasma (CAP) is an ionized gas near room temperature. Its anti-tumor effect can be transmitted either by direct treatment or mediated by a plasma-treated solution (PTS), such as treated standard cell culture medium, which contains different amino acids, inorganic salts, vitamins and other substances. Despite extensive research, the active components in PTS and its molecular or cellular mechanisms are not yet fully understood. The purpose of this study was the measurement of the reactive species in PTS and their effect on tumor cells using different plasma modes and treatment durations. The PTS analysis yielded mode- and dose-dependent differences in the production of reactive oxygen and nitrogen species (RONS), and in the decomposition and modification of the amino acids Tyrosine (Tyr) and Tryptophan (Trp). The Trp metabolites Formylkynurenine (FKyn) and Kynurenine (Kyn) were produced in PTS with the 4 kHz (oxygen) mode, inducing apoptosis in Mel Im melanoma cells. Nitrated derivatives of Trp and Tyr were formed in the 8 kHz (nitrogen) mode, elevating the p16 mRNA expression and senescence-associated ß-Galactosidase staining. In conclusion, the plasma mode has a strong impact on the composition of the active components in PTS and affects its anti-tumor mechanism. These findings are of decisive importance for the development of plasma devices and the effectiveness of tumor treatment.

scholarly journals Acidified Nitrite Contributes to the Antitumor Effect of Cold Atmospheric Plasma on Melanoma Cells

2021 ◽  
Vol 22 (7) ◽  
pp. 3757
Author(s):  
Tom Zimmermann ◽  
Lisa A Gebhardt ◽  
Lucas Kreiss ◽  
Christin Schneider ◽  
Stephanie Arndt ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Membrane Damage ◽  
Melanoma Cells ◽  
Atmospheric Plasma ◽  
Active Components ◽  
Antitumor Effects ◽  
Cold Atmospheric Plasma ◽  
Viability Analysis ◽  
Cellular Effects ◽  
The Impact

Cold atmospheric plasma (CAP) is partially ionized gas near room temperature with previously reported antitumor effects. Despite extensive research and growing interest in this technology, active components and molecular mechanisms of CAP are not fully understood to date. We used Raman spectroscopy and colorimetric assays to determine elevated nitrite and nitrate levels after treatment with a MiniFlatPlaster CAP device. Previously, we demonstrated CAP-induced acidification. Cellular effects of nitrite and strong extracellular acidification were assessed using live-cell imaging of intracellular Ca2+ levels, cell viability analysis as well as quantification of p21 and DNA damage. We further characterized these observations by analyzing established molecular effects of CAP treatment. A synergistic effect of nitrite and acidification was found, leading to strong cytotoxicity in melanoma cells. Interestingly, protein nitration and membrane damage were absent after treatment with acidified nitrite, thereby challenging their contribution to CAP-induced cytotoxicity. Further, phosphorylation of ERK1/2 was increased after treatment with both acidified nitrite and indirect CAP. This study characterizes the impact of acidified nitrite on melanoma cells and supports the importance of RNS during CAP treatment. Further, it defines and evaluates important molecular mechanisms that are involved in the cancer cell response to CAP.

Cold atmospheric plasma, a new strategy to induce senescence in melanoma cells

2013 ◽  
Vol 22 (4) ◽  
pp. 284-289 ◽  
Author(s):  
Stephanie Arndt ◽  
Eva Wacker ◽  
Yang-Fang Li ◽  
Tetsuji Shimizu ◽  
Hubertus M. Thomas ◽  
...  
Keyword(s):  
Melanoma Cells ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma ◽  
New Strategy

scholarly journals Overview of Cold Atmospheric Plasma in Wounds Treatment

2020 ◽  
Vol 5 (10) ◽  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Atmospheric Plasma ◽  
Active Components ◽  
Negative Effects ◽  
Skin Wound Healing ◽  
Cold Atmospheric Plasma ◽  
In Vivo Experiments

Cold atmospheric plasma (CAP), a room temperate ionised gas, known as the fourth state of matter is an ionised gas and can be produced from argon, helium, nitrogen, oxygen or air at atmospheric pressure and low temperatures. CAP has become a new promising way for many biomedical applications, such as disinfection, cancer treatment, root canal treatment, wound healing, and other medical applications. Among these applications, investigations of plasma for skin wound healing have gained huge success both in vitro and in vivo experiments without any known significant negative effects on healthy tissues. The development of CAP devices has led to novel therapeutic strategies in wound healing, tissue regeneration and skin infection management. CAP consists of a mixture of multitude of active components such as charged particles, electric field, UV radiation, and reactive gas species which can act synergistically. CAP has lately been recognized as an alternative approach in medicine for sterilization of wounds by its antiseptic effects and promotion of wound healing by stimulation of cell proliferation and migration of wound related skin cells. With respect to CAP applications in medicine, this review focuses particularly on the potential of CAP and the known molecular basis for this action. We summarize the available literature on the plasma devices developed for wound healing, the current in vivo and in vitro use of CAP, and the mechanism behind it as well as the biosafety issues.

scholarly journals Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Christin Schneider ◽  
Lisa Gebhardt ◽  
Stephanie Arndt ◽  
Sigrid Karrer ◽  
Julia L. Zimmermann ◽  
...  
Keyword(s):  
Calcium Influx ◽  
Melanoma Cells ◽  
Atmospheric Plasma ◽  
Cold Atmospheric Plasma

scholarly journals Anti-Melanoma Capability of Contactless Cold Atmospheric Plasma Treatment

2021 ◽  
Vol 22 (21) ◽  
pp. 11728
Author(s):  
Dayun Yan ◽  
Qihui Wang ◽  
Xiaoliang Yao ◽  
Alisa Malyavko ◽  
Michael Keidar
Keyword(s):  
Tumor Therapy ◽  
Treatment Method ◽  
Melanoma Cells ◽  
Atmospheric Plasma ◽  
Operational Parameter ◽  
Cold Atmospheric Plasma ◽  
Non Invasive ◽  
Metal Sheets ◽  
Physically Based ◽  
Solid Foundation

In this study, we demonstrated that the widely used cold atmospheric plasma (CAP) jet could significantly inhibit the growth of melanoma cells using a contactless treatment method, The flow rate of helium gas was a key operational parameter to modulate electromagnetic (EM) effect on melanoma cells. Metal sheets with different sizes could be used as a strategy to control the strength of EM effect. More attractive, the EM effect from CAP could penetrate glass/polystyrene barriers as thick as 7 mm. All these discoveries presented the profound non-invasive nature of a physically based CAP treatment, which provided a solid foundation for CAP-based cutaneous/subcutaneous tumor therapy.

scholarly journals Cold Atmospheric Plasma Is a Potent Tool to Improve Chemotherapy in Melanoma In Vitro and In Vivo

Biomolecules ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1011 ◽  
Author(s):  
Mina Alimohammadi ◽  
Monireh Golpour ◽  
Farshad Sohbatzadeh ◽  
Seyedehniaz Hadavi ◽  
Sander Bekeschus ◽  
...  
Keyword(s):  
Scientific Evidence ◽  
Argon Plasma ◽  
Melanoma Cells ◽  
Atmospheric Plasma ◽  
Tumor Control ◽  
Substantial Impact ◽  
L929 Cells ◽  
Cold Atmospheric Plasma

Malignant melanoma is a devastating disease. Because of its aggressiveness, it also serves as a model tumor for investigating novel therapeutic avenues. In recent years, scientific evidence has shown that cold atmospheric plasma (CAP) might be a promising modality in cancer therapy. In this study, we aimed to evaluate the effect of CAP generated by an argon plasma jet alone or in combination with dacarbazine (DAC) on melanoma cells in vitro and in vivo. The effects of the CAP on inducing lipid peroxidation and nitric oxide production were higher in B16 melanoma cells in comparison to non-malignant L929 cells. Assays on cell growth, apoptosis, and expression of genes related to, e.g., autophagic processes, showed CAP to have a substantial impact in melanoma cells while there were only minoreffects in L929 cells. In vivo, both CAP monotherapy and combination with DAC significantly decreased tumor growth. These results suggest that CAP not only selectively induces cell death in melanoma but also holds promises in combination with chemotherapy that might lead to improved tumor control.

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