scholarly journals The Role of Processed Aloe vera Gel in Intestinal Tight Junction: An In Vivo and In Vitro Study

2021 ◽  
Vol 22 (12) ◽  
pp. 6515
Author(s):  
Thu Han Le Phan ◽  
Se Yong Park ◽  
Hyun Jin Jung ◽  
Min Woo Kim ◽  
Eunae Cho ◽  
...  
Keyword(s):  
Tight Junction ◽  
Healthy Lifestyle ◽  
Oral Treatment ◽  
Aloe Vera ◽  
In Vitro Study ◽  
Leaky Gut ◽  
Age Related ◽  
Aloe Vera Gel

Leaky gut is a condition of increased paracellular permeability of the intestine due to compromised tight junction barriers. In recent years, this affliction has drawn the attention of scientists from different fields, as a myriad of studies prosecuted it to be the silent culprit of various immune diseases. Due to various controversies surrounding its culpability in the clinic, approaches to leaky gut are restricted in maintaining a healthy lifestyle, avoiding irritating factors, and practicing alternative medicine, including the consumption of supplements. In the current study, we investigate the tight junction-modulating effects of processed Aloe vera gel (PAG), comprising 5–400-kD polysaccharides as the main components. Our results show that oral treatment of 143 mg/kg PAG daily for 10 days improves the age-related leaky gut condition in old mice, by reducing their individual urinal lactulose/mannitol (L/M) ratio. In concordance with in vivo experiments, PAG treatment at dose 400 μg/mL accelerated the polarization process of Caco-2 monolayers. The underlying mechanism was attributed to enhancement in the expression of intestinal tight junction-associated scaffold protein zonula occludens (ZO)-1 at the translation level. This was induced by activation of the MAPK/ERK signaling pathway, which inhibits the translation repressor 4E-BP1. In conclusion, we propose that consuming PAG as a complementary food has the potential to benefit high-risk patients.

Evaluation of Isolated Fractions of Aloe vera Gel Materials on Indinavir Pharmacokinetics: In vitro and in vivo Studies

2016 ◽  
Vol 13 (3) ◽  
pp. 471-480 ◽  
Author(s):  
Lonette Wallis ◽  
Maides Malan ◽  
Chrisna Gouws ◽  
Dewald Steyn ◽  
Suria Ellis ◽  
...  
Keyword(s):  
Aloe Vera ◽  
In Vivo Studies ◽  
Aloe Vera Gel

scholarly journals Comparative Evaluation of Antibacterial Effect of Propolis and Aloe Vera, Xylitol, and Cpp-Acp Gels on Streptococcus mutans and Lactobacillus in Vitro

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Maryam Hajiahmadi ◽  
Jamshid Faghri ◽  
Zohre Salehi ◽  
Fariba Heidari
Keyword(s):  
Streptococcus Mutans ◽  
Antibacterial Effect ◽  
Aloe Vera ◽  
Antibacterial Properties ◽  
In Vitro Study ◽  
Antimicrobial Effect ◽  
Deciduous Teeth ◽  
P Value ◽  
Aloe Vera Gel

Introduction. Early childhood caries is a kind of caries occurring in deciduous teeth. Bacteria are among the main factors. Antibacterial agents such as fluoride are used in both prevention and treatment, but their application in children faces limitations such as fluorosis. Therefore, novel methods of caries prevention among the children are mainly focused on the use of fluoride-free active ingredients. In this comparative study, antibacterial effects of gels containing propolis and aloe vera, fluoride, xylitol, and CPP-ACP were investigated. Methods. This is an in vitro study. By plate well technique, plates containing gels were created in the culture medium of Streptococcus mutans and Lactobacillus, and their antibacterial impacts were evaluated by measuring the inhibition zone after 24, 48, and 72 hours. Then, different concentrations of each gel were evaluated in the same way for the antibacterial properties. For each sample, this process was iterated 3 times, where the average was declared as the final number. The collected data were entered in SPSS 24. Results. In both bacteria, propolis gel and aloe vera had the highest zone of inhibition, followed by fluoride and xylitol in the second and third places, respectively. Different concentrations of gels are significantly different in terms of antimicrobial effect (P value ≤ 0/05). The antimicrobial effect of propolis and aloe vera gel was kept up to the concentration of 1 / 16 . As the bacterial and gel contact time is prolonged, the antibacterial effect of different gels increases, but the difference is not statistically significant (P value = 0.109). CPP-ACP gel had no antimicrobial effect at any concentration. Conclusion. Propolis and aloe vera gel had a greater antimicrobial effect than other gels, where such effect was observed in low concentrations. CPP-ACP gel had no antimicrobial properties.

scholarly journals EVALUATION OF BIOLOGICAL ACTIVITY OF GREENLY SYNTHESIZED SILVER NANOPARTICLES USING ALOE VERA GEL EXTRACT AS ANTIBACTERIAL AGENT IN-VITRO AND IN-VIVO

2020 ◽  
Vol 51 (6) ◽  
pp. 1706-1715
Author(s):  
Ibrahim & et al.
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Methicillin Resistance ◽  
Aloe Vera ◽  
Dilution Method ◽  
Lesion Volume ◽  
Aloe Vera Gel ◽  
Complicated Skin

The present study was carried out to synthesis silver nanoparticles by using Aloe Vera gel and evaluates antibacterial activity in vitro and in vivo. The synthesis and characterization of silver nanoparticles was confirmed by Ultra Violet Visible- spectrophotometer, X-ray diffraction, Fourier Transmission Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy. Reduction of the Ag+ to Ag0 during exposure to the Aloe Vera gel extract was followed by color change of the solution from colorless, yellow to dark brown within 24 hours. It is observed that surface Plasmon resonance peaks of the maximum absorbance of silver-nanoparticles occur at 425 nm, indicating that AgNPs were produced. Later on, using agar well diffusion and tube dilution method against pathogenic methicillin resistance staphylococcus aureus, (MRSA). Pseudomonas. aeruginosa and Escherichia coli, the antimicrobial properties of synthesised AgNPs were investigated. To confirm in-vivo antibacterial activity, after inducing complicated skin and soft tissue infection in mice by injecting S.aureus subcutaneously. 16µg/gm AgNPs were applied skin infected daily for three days. Silver nanoparticles were as succeeded to reduce the lesion volume in infected mice and reduce the acute inflammation symptoms as clindamycin, In conclusion, A new approach can be used to combat serious infections caused by MRSA by Aloe Vera AgNPs.

An in vitro and in vivo toxicologic evaluation of a stabilized aloe vera gel supplement drink in mice

2013 ◽  
Vol 55 ◽  
pp. 363-370 ◽  
Author(s):  
Inder Sehgal ◽  
Wallace D. Winters ◽  
Michael Scott ◽  
Konstantine Kousoulas
Keyword(s):  
Aloe Vera ◽  
Aloe Vera Gel
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