scholarly journals Silica Particles Trigger the Exopolysaccharide Production of Harsh Environment Isolates of Growth-Promoting Rhizobacteria and Increase Their Ability to Enhance Wheat Biomass in Drought-Stressed Soils

2021 ◽  
Vol 22 (12) ◽  
pp. 6201
Author(s):  
Anastasiia Fetsiukh ◽  
Julian Conrad ◽  
Jonas Bergquist ◽  
Salme Timmusk
Keyword(s):  
Osmotic Pressure ◽  
Large Scale ◽  
Plant Biomass ◽  
Plant Growth Promoting Rhizobacteria ◽  
Silica Particles ◽  
Electron Microscopic ◽  
Bacterial Morphology ◽  
Microscopic Studies ◽  
Plant Growth Promoting ◽  
Growth Promoting

In coming decades, drought is expected to expand globally owing to increased evaporation and reduced rainfall. Understanding, predicting, and controlling crop plants’ rhizosphere has the potential to manipulate its responses to environmental stress. Our plant growth-promoting rhizobacteria (PGPR) are isolated from a natural laboratory, ‘The Evolution Canyon’, Israel, (EC), from the wild progenitors of cereals, where they have been co-habituating with their hosts for long periods of time. The study revealed that commercial TM50 silica particles (SN) triggered the PGPR production of exopolysaccharides (EPS) containing D-glucuronate (D-GA). The increased EPS content increased the PGPR water-holding capacity (WHC) and osmotic pressure of the biofilm matrix, which led to enhanced plant biomass in drought-stressed growth environments. Light- and cryo-electron- microscopic studies showed that, in the presence of silica (SN) particles, bacterial morphology is changed, indicating that SNs are associated with significant reprogramming in bacteria. The findings encourage the development of large-scale methods for isolate formulation with natural silicas that ensure higher WHC and hyperosmolarity under field conditions. Osmotic pressure involvement of holobiont cohabitation is also discussed.

scholarly journals Silica particles trigger the production of exopolysaccharides in harsh environment plant growth-promoting rhizobacteria and increase their ability to enhance drought tolerance

2020 ◽  
Author(s):  
Anastasiia Fetsiukh ◽  
Julian Conrad ◽  
Jonas Bergquist ◽  
Fantaye Ayele ◽  
Salme Timmusk
Keyword(s):  
Plant Growth ◽  
Osmotic Pressure ◽  
Plant Biomass ◽  
Plant Growth Promoting Rhizobacteria ◽  
Silica Particles ◽  
Natural Soil ◽  
Plant Responses ◽  
Growth Environment ◽  
Plant Growth Promoting ◽  
Growth Promoting

ABSTRACTIn coming decades drought is expected to expand globally owing to increased evaporation and reduced rainfall. In order to reduce the vulnerability of agricultural systems we need to understand the crop plant growth environment. Understanding, predicting and controlling the rhizosphere has potential to harness plant microbe interactions, improve plant responses to environmental stress and mitigate effects of climate change. Our plant growth-promoting rhizobacteria (PGPR) are isolated from the natural laboratory ‘Evolution Canyon’ Israel (EC). The endophytic rhizobacteria from the wild progenitors of cereals have been co-habituated with their hosts for long periods of time. The study revealed that silica particles (SN) triggered the PGPR production of exopolysaccharides (EPS) containing D-glucuronate (D-GA). This leads to increased plant biomass accumulation in drought-stressed growth environments. The PGPR increased EPS content increases the water holding capacity (WHC) and osmotic pressure of the biofilm matrix. Light- and electron-microscopic studies show that in the presence of SN particles, bacterial morphology is changed, indicating that SNs are associated with significant reprogramming in bacteria.The results here show that the production of EPS containing D-GA is induced by SN treatment. The findings encourage formulation of cells considering microencapsulation with materials that ensure higher WHC and hyperosmolarity under field conditions. Our results illustrate the importance of considering natural soil nanoparticles in the application of PGPR. Osmotic pressure involvement of holobiont cohabitation is discussed.

scholarly journals Impact of Plant Growth-Promoting Rhizobacteria Inoculation and Grafting on Tolerance of Tomato to Combined Water and Nutrient Stress Assessed via Metabolomics Analysis

2021 ◽  
Vol 12 ◽  
Author(s):  
Panagiotis Kalozoumis ◽  
Dimitrios Savvas ◽  
Konstantinos Aliferis ◽  
Georgia Ntatsi ◽  
George Marakis ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Biomass ◽  
Plant Growth Promoting Rhizobacteria ◽  
Combined Stress ◽  
Nitrogen And Phosphorus ◽  
Screening Process ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
As Stress ◽  
Metabolomics Analysis

In the current study, inoculation with plant growth-promoting rhizobacteria (PGPR) and grafting were tested as possible cultural practices that may enhance resilience of tomato to stress induced by combined water and nutrient shortage. The roots of tomato grown on perlite were either inoculated or not with PGPR, applying four different treatments. These were PGPR-T1, a mix of two Enterobacter sp. strains (C1.2 and C1.5); PGPR-T2, Paenibacillus sp. strain DN1.2; PGPR-T3, Enterobacter mori strain C3.1; and PGPR-T4, Lelliottia sp. strain D2.4. PGPR-treated plants were either self-grafted or grafted onto Solanum lycopersicum cv. M82 and received either full or 50% of their standard water, nitrogen, and phosphorus needs. The vegetative biomass of plants subjected to PGPR-T1 was not reduced when plants were cultivated under combined stress, while it was reduced by stress to the rest of the PGPR treatments. However, PGPR-T3 increased considerably plant biomass of non-stressed tomato plants than did all other treatments. PGPR application had no impact on fruit biomass, while grafting onto ’M82’ increased fruit production than did self-grafting. Metabolomics analysis in tomato leaves revealed that combined stress affects several metabolites, most of them already described as stress-related, including trehalose, myo-inositol, and monopalmitin. PGPR inoculation with E. mori strain C3.1 affected metabolites, which are important for plant/microbe symbiosis (myo-inositol and monopalmitin). The rootstock M82 did not affect many metabolites in plant leaves, but it clearly decreased the levels of malate and D-fructose and imposed an accumulation of oleic acid. In conclusion, PGPR are capable of increasing tomato tolerance to combined stress. However, further research is required to evaluate more strains and refine protocols for their application. Metabolites that were discovered as biomarkers could be used to accelerate the screening process for traits such as stress tolerance to abiotic and/or abiotic stresses. Finally, ‘M82’ is a suitable rootstock for tomato, as it is capable of increasing fruit biomass production.

scholarly journals Effects of Plant Growth Promoting Rhizobacteria Microbial Inoculants on the Growth, Rhizosphere Soil Properties, and Bacterial Community of Pinus sylvestris var. mongolica Annual Seedlings

2020 ◽  
Author(s):  
Qian Song ◽  
Xiaoshuang Song ◽  
Xun Deng ◽  
Jiayu Luo ◽  
Junkai Wang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Plant Growth ◽  
Physicochemical Properties ◽  
Plant Biomass ◽  
Soil Nutrient ◽  
Plant Growth Promoting Rhizobacteria ◽  
Inorganic Phosphorus ◽  
Nutrient Index ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract [Objective] Determine the ability of three plant growth promoting rhizobacteria (PGPR) strains (Pseudomonas Mandelli A36, Serratia plymuthica A13 and Pseudomonas koreensis A20) to promote plant growth, evaluate the effect of inoculation with PGPR strains on seedling biomass, root structure, nutrient index, and enzyme activity, and assess the effect of PGPR inoculation on soil nutrient index, enzyme activity, and the soil microecological environment.[Method] The ability of the three PGPR strains to secrete indole-3-acetic acid (IAA), dissolve inorganic phosphorus, and produce siderophore and hydrolase was determined by the medium color change method, pot experiment to determine the effects of three PGPR strains on plant biomass, physicochemical properties, soil physicochemical properties and microbial diversity. [Result] The three PGPR strains had the ability to secrete IAA, solubilize inorganic phosphorus, and produce siderophore, the results of the pot experiment showed that inoculation with PGPR strain had a significant effect on plant biomass, root index, nutrient index and enzyme activity, as well as soil nutrient index, enzyme activity and bacterial diversity. [Conclusion] This study provides basic data references for PGPR strains to improve the soil microecological environment and promote the growth and development of Pinus sylvestris var. Mongolica seedlings.

scholarly journals Influence of Nitrogen Sources and Plant Growth-Promoting Rhizobacteria Inoculation on Growth, Crude Fiber and Nutrient Uptake in Squash (Cucurbita moschata Duchesne ex Poir.) Plants

2016 ◽  
Vol 44 (1) ◽  
pp. 53-59 ◽  
Author(s):  
Alice I. TCHIAZE ◽  
Victor D. TAFFOUO ◽  
Henri FANKEM ◽  
Martin KENNE ◽  
Régis BAZIRAMAKENGA ◽  
...  
Keyword(s):  
Plant Growth ◽  
Nutrient Uptake ◽  
Plant Biomass ◽  
Growth Parameters ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Crude Fiber ◽  
Cellulose Content ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth promoting rhizobacteria (PGPR, B) have immense potential application in sustainable agriculture as ecofriendly biofertilizers and biopesticides. In this study, the effects of three nitrogen (N) sources (NO3-, NH4+ and NO3NH4) and PGPR on growth, crude fiber and nutrient uptake were investigated in squash plants. Some growth parameters [root dry weight (RDW), shoot dry weight (SDW), total plant dry weight (PDW), number of leaves (NL), shoot length (SL), stem diameter (SD) and number of ramifications (NR)], crude fiber (cellulose content) and nutrient uptake (N, P, K, Ca, Mg, Na, Fe, Cu, Mn and Zn) were determined. Application of NO3-, NH4+ or NO3NH4 singly or in combination with PGPR inoculation led to a significant increase in RDW, SDW, PDW, NL, SL, SD and NR. Na, Cu and Zn contents, on the contrary, decreased in inoculated treated plants while no significant differences were recorded in cellulose contents (CE) of leaves except in plants fed with NO3-. The leaf CE content ranged from 12.58 to 13.67%. The plants supplied with NO3+B, NH4+B and NO3NH4+B showed significantly higher plant biomass and accumulation of N, P, K and Mn concentrations in leaves compared to all other treatments. These results suggest that specific combinations of PGPR with NO3-, NH4+ or NO3NH4 fertilizers can be considered as efficient alternative biofertilizers to improve significantly the squash growth and nutrient uptake.

scholarly journals The Azospirillum genus and the cultivation of vegetables. A review

2021 ◽  
pp. 236-246
Author(s):  
Eduardo Pradi Vendruscolo ◽  
Sebastião Ferreira de Lima
Keyword(s):  
Plant Growth ◽  
Environmental Protection ◽  
Agricultural Production ◽  
Large Scale ◽  
Environmentally Friendly ◽  
Plant Growth Promoting Rhizobacteria ◽  
Grain Production ◽  
Agricultural Producers ◽  
Plant Growth Promoting ◽  
Growth Promoting

Introduction. Plant growth-promoting rhizobacteria have been successfully inserted in agriculture, aiming to find a better balance between agricultural production and environmental protection. However, there is a restriction on its application concerning the exploited species since the focus is on large-scale grain production, practically excluding small and medium-sized farms. Literature. Studies on the application of Azospirillum bacteria in horticultural species are still scarce, compared with those aimed at grain production. However, it appears that these bacteria are beneficial to the development and production of vegetables, whether they produce leaves, stems, bulbs, flowers, fruits, roots, or tubers, and may result in monetary gains, especially for small and medium agricultural producers. Conclusions. The use of Azospirillum bacteria to increase the quality and quantity of products from horticultural species and establish an environmentally friendly practice is a reality. However, the development of research that defines the best strategies for using this technology must be carried out continuously, aiming at the best conditions for producers.

scholarly journals Improved chromium tolerance of Medicago sativa by plant growth-promoting rhizobacteria (PGPR)

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Nabil Tirry ◽  
Aziza Kouchou ◽  
Bouchra El Omari ◽  
Mohamed Ferioun ◽  
Naïma El Ghachtouli
Keyword(s):  
Plant Growth ◽  
Medicago Sativa ◽  
Contaminated Soils ◽  
Plant Biomass ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Biochemical Tests ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background Soil pollution by heavy metals increases the bioavailability of metals like hexavalent chromium (Cr (VI)), subsequently limiting plant growth and reducing the efficiency of phytoremediation. Plant growth-promoting rhizobacteria (PGPR) have substantial potential to enhance plant growth as well as plant tolerance to metal stress. The aim of this research was to investigate Cr (VI) phytoremediation enhancement by PGPR. Results The results showed that the 27 rhizobacterial isolates studied were confirmed as Cr (VI)-resistant PGPR, by using classical biochemical tests (phosphate solubilization, nitrogen fixation, indole acetic acid, exopolysaccharides, hydrogen cyanide, siderophores, ammonia, cellulase, pectinase, and chitinase production) and showed variable levels of Cr (VI) resistance (300–600 mg/L). The best four selected Cr (VI)-resistant PGPR (NT15, NT19, NT20, and NT27) retained most of the PGP traits in the presence of 100–200 mg/L concentrations of Cr (VI). The inoculation of Medicago sativa with any of these four isolates improved the shoot and root dry weight. The NT27 isolate identified using 16S rDNA gene sequence analyses as a strain of Pseudomonas sp. was most effective in terms of plant growth promotion and stress level decrease. It increased shoot and root dry weights of M. sativa by 97.6 and 95.4%, respectively, in the presence of Cr (VI) when compared to non-inoculated control plants. It also greatly increased chlorophyll content and decreased the levels of stress markers, malondialdehyde, hydrogen peroxide, and proline. The results of the effect of Pseudomonas sp. on Cr content and bioaccumulation factor (BAF) of the shoots and roots of M. sativa plants showed the increase of plant biomass concomitantly with the increase of Cr root concentration in inoculated plants. This would lead to a higher potential of Cr (VI) phytostabilization. Conclusions This study demonstrates that the association M. sativa-Pseudomonas sp. may be an efficient biological system for the bioremediation of Cr (VI)-contaminated soils.

Characterization of plant growth-promoting rhizobacterium Exiguobacterium NII-0906 for its growth promotion of cowpea (Vigna unguiculata)

Biologia ◽  
2010 ◽  
Vol 65 (2) ◽  
Author(s):  
Syed Dastager ◽  
Deepa Kumaran ◽  
Ashok Pandey
Keyword(s):  
Plant Growth ◽  
Plant Biomass ◽  
Growth Promotion ◽  
Plant Root ◽  
Western Ghat ◽  
Electron Microscopic ◽  
16S Rrna Analysis ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing

AbstractA phosphate solubilizing and antagonistic bacterial strain, isolated from a Western Ghat forest soil in Kerala province, India (designated as NII-0906), showed cold tolerance and grew from 10 to 37°C (optimum temperature 30°C). It was a Gram-positive, rod shaped, 0.8–1.6 μm in size, and exhibited tolerance to a wide pH range (5–12; optimum 7.0) and salt concentration up to 7% (w/v). The isolate showed maximum similarity with Exiguobacterium marinum TF-80T based on 16S rRNA analysis. It solubilized tricalcium phosphate under in vitro conditions. The phosphate solubilization was estimated along a temperature range (5–40°C), and maximum activity (84.7 μg mL−1 day−1) was recorded at 30°C after 10 days of incubation. The phosphate solubilizing activity coincided with a concomitant decrease in pH of the medium. The isolate also exhibited antifungal activity against phytopathogenic fungi in Petri dish assays and produced siderophore and hydrogen cyanide. The strain’s plant growth promotion properties were demonstrated through a cowpea-based bioassay under greenhouse conditions. The bacterial inoculation resulted in significant increment in plant root, stem and as well as in plant biomass. Further, scanning electron microscopic study revealed the root colonization in cowpea. These results could offer potential perspective for the strain to be used as plant growth-promoting rhizobacteria, which could be used as an inoculant for regional crops.

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Capsicum Frutescens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

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