scholarly journals Prussian Blue: A Nanozyme with Versatile Catalytic Properties

2021 ◽  
Vol 22 (11) ◽  
pp. 5993
Author(s):  
Joan Estelrich ◽  
M. Antònia Busquets
Keyword(s):  
Prussian Blue ◽  
Catalytic Properties ◽  
Catalytic Performance ◽  
Excellent Performance ◽  
Catalytic Activities ◽  
Prussian Blue Nanoparticles ◽  
In Vivo Diagnostics ◽  
Metal Organic ◽  
Highly Porous

Nanozymes, nanomaterials with enzyme-like activities, are becoming powerful competitors and potential substitutes for natural enzymes because of their excellent performance. Nanozymes offer better structural stability over their respective natural enzymes. In consequence, nanozymes exhibit promising applications in different fields such as the biomedical sector (in vivo diagnostics/and therapeutics) and the environmental sector (detection and remediation of inorganic and organic pollutants). Prussian blue nanoparticles and their analogues are metal–organic frameworks (MOF) composed of alternating ferric and ferrous irons coordinated with cyanides. Such nanoparticles benefit from excellent biocompatibility and biosafety. Besides other important properties, such as a highly porous structure, Prussian blue nanoparticles show catalytic activities due to the iron atom that acts as metal sites for the catalysis. The different states of oxidation are responsible for the multicatalytic activities of such nanoparticles, namely peroxidase-like, catalase-like, and superoxide dismutase-like activities. Depending on the catalytic performance, these nanoparticles can generate or scavenge reactive oxygen species (ROS).

scholarly journals Directed Evolution of a Homodimeric Laccase from Cerrena unicolor BBP6 by Random Mutagenesis and In Vivo Assembly

2018 ◽  
Vol 19 (10) ◽  
pp. 2989 ◽  
Author(s):  
Ji Zhang ◽  
Fuying Ma ◽  
Xiaoyu Zhang ◽  
Anli Geng
Keyword(s):  
Directed Evolution ◽  
Laccase Activity ◽  
Catalytic Properties ◽  
Random Mutagenesis ◽  
Catalytic Performance ◽  
Industrial Applications ◽  
Subunit Interaction ◽  
Cerrena Unicolor ◽  
Protein Model

Laccases have great potential for industrial applications due to their green catalytic properties and broad substrate specificities, and various studies have attempted to improve the catalytic performance of these enzymes. Here, to the best of our knowledge, we firstly report the directed evolution of a homodimeric laccase from Cerrena unicolor BBP6 fused with α-factor prepro-leader that was engineered through random mutagenesis followed by in vivo assembly in Saccharomyces cerevisiae. Three evolved fusion variants selected from ~3500 clones presented 31- to 37-fold increases in total laccase activity, with better thermostability and broader pH profiles. The evolved α-factor prepro-leader enhanced laccase expression levels by up to 2.4-fold. Protein model analysis of these variants reveals that the beneficial mutations have influences on protein pKa shift, subunit interaction, substrate entrance, and C-terminal function.

Synthesis and catalytic properties of trans-A2B2-type metalloporphyrins in cyclohexane oxidation

2014 ◽  
Vol 92 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Yujia Xie ◽  
Fengyong Zhang ◽  
Pingle Liu ◽  
Fang Hao ◽  
He’an Luo
Keyword(s):  
Metal Ions ◽  
Catalytic Properties ◽  
Condensation Reaction ◽  
Catalytic Performance ◽  
Cyclohexane Oxidation ◽  
Central Metal ◽  
Reaction Conditions ◽  
Catalytic Activities ◽  
Cobalt Nickel

A new efficient and mild protocol for synthesizing a series of trans-A2B2-porphyrins through a TFA-catalyzed condensation reaction between various aldehydes and dipyrromethanes has been developed. Several trans-A2B2-metalloporphyrins (cobalt, nickel) were synthesized and used to catalyze cyclohexane oxidation C–H bonds with dioxygen in the absence of additives and solvents. The results show that the catalytic activities were relative to the nature of the substituted groups and the central metal ions of trans-A2B2-metalloporphyrin. Cobalt metalloporphyrin presents better catalytic performance in the conversion of cyclohexane than the nickel metalloporphyrin under the same reaction conditions.

scholarly journals Mn doped Prussian blue nanoparticles for T1/T2 MR imaging, PA imaging and Fenton reaction enhanced mild temperature photothermal therapy of tumor

2022 ◽  
Vol 20 (1) ◽  
Author(s):  
Quan Tao ◽  
Genghan He ◽  
Sheng Ye ◽  
Di Zhang ◽  
Zhide Zhang ◽  
...  
Keyword(s):  
Prussian Blue ◽  
Photothermal Therapy ◽  
Multimodal Imaging ◽  
Fenton Reaction ◽  
Infrared Range ◽  
Prussian Blue Nanoparticles ◽  
Mild Temperature ◽  
Mn Doped

Abstract Background Combining the multimodal imaging and synergistic treatment in one platform can enhance the therapeutic efficacy and diagnosis accuracy. Results In this contribution, innovative Mn-doped Prussian blue nanoparticles (MnPB NPs) were prepared via microemulsion method. MnPB NPs demonstrated excellent T1 and T2 weighted magnetic resonance imaging (MRI) enhancement in vitro and in vivo. The robust absorbance in the near infrared range of MnPB NPs provides high antitumor efficacy for photothermal therapy (PTT) and photoacoustics imaging property. Moreover, with the doping of Mn, MnPB NPs exhibited excellent Fenton reaction activity for chemodynamic therapy (CDT). The favorable trimodal imaging and Fenton reaction enhanced mild temperature photothermal therapy in vitro and in vivo were further confirmed that MnPB NPs have significant positive effectiveness for integration of diagnosis and treatment tumor. Conclusions Overall, this Mn doped Prussian blue nanoplatform with multimodal imaging and chemodynamic/mild temperature photothermal co-therapy provides a reliable tool for tumor treatment. Graphical Abstract

Biosafety and biocompatibility assessment of Prussian blue nanoparticles in vitro and in vivo

Nanomedicine ◽  
2020 ◽  
Vol 15 (27) ◽  
pp. 2655-2670
Author(s):  
Zhou Wang ◽  
Ying Long ◽  
Jialong Fan ◽  
Chang Xiao ◽  
Chunyi Tong ◽  
...  
Keyword(s):  
Renal Function ◽  
Prussian Blue ◽  
Peroxidase Activity ◽  
Positive Charge ◽  
Morphological Characteristics ◽  
Prussian Blue Nanoparticles ◽  
In Vivo Analysis ◽  
Good Biocompatibility

Aim: To investigate the effects of the different morphological characteristics of Prussian blue nanoparticles (PB NPs) on their biocompatibility and biosafety. Materials & methods: PB NPs with different sizes, shapes and charges were synthesized and their biosafety and biocompatibility performance were systematically compared in vitro and in vivo. Results: Increased size and positive charge of PB NPs adversely affected cell viability, while improving their peroxidase activity and photothermal conversion efficiency. In vivo analysis demonstrated good biocompatibility of PB NPs, without retention in the organs, but increased size retarded their metabolism. Meanwhile, increased size and positive charge adversely affected hepatic and renal function. Conclusion: This comprehensive exploration of biosafety and biocompatibility provides strong evidences for the use of PB NPs as nanodrug carrier and/or imaging agent.

In situ synthesis of Prussian blue nanoparticles within a biocompatible reverse micellar system for in vivo Cs+uptake

2017 ◽  
Vol 41 (8) ◽  
pp. 2887-2890 ◽  
Author(s):  
Cyril Lavaud ◽  
Marilyn Kajdan ◽  
Elsa Compte ◽  
Jean-Claude Maurel ◽  
Joséphine Lai Kee Him ◽  
...  
Keyword(s):  
Prussian Blue ◽  
High Efficiency ◽  
In Situ Synthesis ◽  
Dose Effect ◽  
Micellar System ◽  
Prussian Blue Nanoparticles ◽  
Reverse Micellar System ◽  
Reverse Micellar

A Prussian blue reverse micellar system forin vivoCs+uptake exhibiting high efficiency and a significant dose effect.

Toxicological evaluation of Prussian blue nanoparticles after short exposure of mice

2016 ◽  
Vol 35 (10) ◽  
pp. 1123-1132 ◽  
Author(s):  
Y Chen ◽  
L Wu ◽  
Q Wang ◽  
M Wu ◽  
B Xu ◽  
...  
Keyword(s):  
T Cells ◽  
Prussian Blue ◽  
Short Exposure ◽  
Toxicological Evaluation ◽  
Prussian Blue Nanoparticles ◽  
High Level ◽  
Histological Results ◽  
Short Time

Prussian blue nanoparticle (PBNP), a new type of theranostic nanomaterial, had been used for cancer magnetic resonance imaging and photothermal therapy. However, their long-term toxicity after short exposure in vivo was still unclear. In this study, we investigated the dynamic changes of the biochemical and immunity indicators of mice after PBNPs injection through tail vein. Histological results showed that the PBNPs were mainly accumulated in liver and spleen. In the spleen, we found the frequency of T cells was starting to decrease after 1 day of PBNPs injection, but then slowly recovered to normal level after 60 days of injection. Meanwhile, the frequency of T cells in the blood was firstly decreased after the PBNPs injection, and then the T cell frequency kept increasing and recovered back to normal levels after 7 days of injection. The serum indexes of liver functions (alanine transaminase, aspartate transaminase, total bilirubin, and alkaline phosphatase) increased rapidly to a relatively high level only after 1 h of injection, which meant certain acute liver damage, but these indexes were gradually decreased to normal levels after 60 days of injection. These results indicate that PBNPs have acute toxicity in vivo, however, their long-term toxicity after short-time exposure is low, which might provide guidance for further applications of PBNPs in future.

201Tl-labeled Prussian blue and Au@Prussian blue nanoprobes for SPEC-CT imaging: influence of the size, shape and coating on the biodistribution

2017 ◽  
Vol 4 (10) ◽  
pp. 1737-1741 ◽  
Author(s):  
Guillaume Maurin-Pasturel ◽  
Estelle Rascol ◽  
Muriel Busson ◽  
Samuel Sevestre ◽  
Joséphine Lai-Kee-Him ◽  
...  
Keyword(s):  
Prussian Blue ◽  
Ct Imaging ◽  
Core Shell ◽  
Prussian Blue Nanoparticles

201Tl-labeled Prussian blue and core@shell Au@Prussian blue nanoparticles coated with the phospholipidic bilayer and dextran were investigated in vivo as nanoprobes by usingSPECT-CT scintigraphy.

Effect of Silica Promoter on Performance of CuO-ZnO-ZrO2 Catalyst for Methanol Synthesis from CO2 Hydrogenation

2014 ◽  
Vol 556-562 ◽  
pp. 117-122 ◽  
Author(s):  
Miao Yao Jia ◽  
Wen Gui Gao ◽  
Hua Wang ◽  
Yu Hao Wang
Keyword(s):  
Catalytic Activity ◽  
Specific Surface ◽  
Surface Area ◽  
Methanol Synthesis ◽  
Catalytic Properties ◽  
Catalytic Performance ◽  
Precipitation Method ◽  
Test Results ◽  
Excellent Performance ◽  
Co Precipitation

Various CuO-ZnO-ZrO2(CZZ) catalysts for methanol synthesis from CO2 hydrogenation were prepared by co-precipitation method. Small amount of silica was incorporated into CZZ catalyst to produce these modified ternary CZZ catalysts. The effects of silica on physicochemical and catalytic properties were studied by TG-DTG,XRD,BET,N2O chemisorption,H2-TPR,NH3-TPD and CO2-TPD techniques. The results show that the properties of catalysts were strongly influenced by the content of SiO2 used as promoter. The catalytic performance for methanol synthesis from CO2 hydrogenation was evaluated. The test results show that the CZZ catalyst modified with 4 wt.% SiO2 exhibits an optimum catalytic activity. The silica improves the dispersion of CuO and its modified CZZ catalysts exhibits higher specific surface area, which were confirmed to be responsible for excellent performance of the catalysts for methanol synthesis from CO2 hydrogenation.

scholarly journals Thallium Labeled Citrate-Coated Prussian Blue Nanoparticles as Potential Imaging Agent

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Krisztián Szigeti ◽  
Nikolett Hegedűs ◽  
Kitti Rácz ◽  
Ildikó Horváth ◽  
Dániel S. Veres ◽  
...  
Keyword(s):  
Prussian Blue ◽  
Contrast Material ◽  
Mri Contrast Agent ◽  
Mri Contrast ◽  
Potential Measurement ◽  
Force Microscopy ◽  
Prussian Blue Nanoparticles ◽  
Volume Of Interest ◽  
Diagnostic Applications

Background. The aim of this study was to develop and characterize a nanoparticle-based image-contrast platform which is biocompatible, chemically stable, and accessible for radiolabeling with 201Tl. We explored whether this nanoparticle enhanced the T1 signal which might make it an MRI contrast agent as well. Methods. The physical properties of citrate-coated Prussian blue nanoparticles (PBNPs) (iron(II);iron(III);octadecacyanide) doped with 201Tl isotope were characterized with atomic force microscopy, dynamic light scattering, and zeta potential measurement. PBNP biodistribution was determined by using SPECT and MRI following intravenous administration into C57BL6 mice. Activity concentrations (MBq/cm3) were calculated from the SPECT scans for each dedicated volume of interest (VOI) of liver, kidneys, salivary glands, heart, lungs, and brain. Results. PBNP accumulation peaked at 2 hours after injection predominantly in the kidneys and the liver followed by a gradual decrease in activity in later time points. Conclusion. We synthetized, characterized, and radiolabeled a Prussian blue-based nanoparticle platform for contrast material applications. Its in vivo radiochemical stability and biodistribution open up the way for further diagnostic applications.

Prussian blue nanoparticles encapsulated inside a metal–organic framework via in situ growth as promising peroxidase mimetics for enzyme inhibitor screening

2016 ◽  
Vol 4 (1) ◽  
pp. 128-134 ◽  
Author(s):  
Linjing Su ◽  
Yuhao Xiong ◽  
Haiguan Yang ◽  
Peng Zhang ◽  
Fanggui Ye
Keyword(s):  
Prussian Blue ◽  
Enzyme Inhibitor ◽  
Metal Organic Framework ◽  
In Situ Growth ◽  
Inhibitor Screening ◽  
Prussian Blue Nanoparticles ◽  
Metal Organic ◽  
Organic Framework

Metal–organic framework-based peroxidase mimetics for enzyme-inhibitor screening.

Sign in / Sign up

Export Citation Format

Share Document