scholarly journals Application of Nitrate, Ammonium, or Urea Changes the Concentrations of Ureides, Urea, Amino Acids and Other Metabolites in Xylem Sap and in the Organs of Soybean Plants (Glycine max (L.) Merr.)

2021 ◽  
Vol 22 (9) ◽  
pp. 4573
Author(s):  
Yuki Ono ◽  
Masashige Fukasawa ◽  
Kuni Sueyoshi ◽  
Norikuni Ohtake ◽  
Takashi Sato ◽  
...  
Keyword(s):  
Glycine Max ◽  
Root Nodules ◽  
Xylem Sap ◽  
Nitrate Treatment ◽  
First Finding ◽  
Metabolite Concentrations ◽  
Glycine Max L ◽  
Soybean Plants ◽  
Positive Correlations ◽  
Ureide Degradation

Soybean (Glycine max (L.) Merr.) plants form root nodules and fix atmospheric dinitrogen, while also utilizing the combined nitrogen absorbed from roots. In this study, nodulated soybean plants were supplied with 5 mM N nitrate, ammonium, or urea for 3 days, and the changes in metabolite concentrations in the xylem sap and each organ were analyzed. The ureide concentration in the xylem sap was the highest in the control plants that were supplied with an N-free nutrient solution, but nitrate and asparagine were the principal compounds in the xylem sap with nitrate treatment. The metabolite concentrations in both the xylem sap and each organ were similar between the ammonium and urea treatments. Considerable amounts of urea were present in the xylem sap and all the organs among all the treatments. Positive correlations were observed between the ureides and urea concentrations in the xylem sap as well as in the roots and leaves, although no correlations were observed between the urea and arginine concentrations, suggesting that urea may have originated from ureide degradation in soybean plants, possibly in the roots. This is the first finding of the possibility of ureide degradation to urea in the underground organs of soybean plants.

scholarly journals Potensi Jenis Bahan Organik Sebagai Biostimulan dalam Meningkatkan Populasi Azospirillum sp, dan Hasil Kedelai (Glycine Max. L.) pada Inceptisol Jatinangor

SoilREns ◽  
2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Pujawati Suryatmana ◽  
Silmi Rahadiana Putri ◽  
Nadia Nuraniya Kamaluddin ◽  
Mieke Rochimi Setiawati
Keyword(s):  
Glycine Max ◽  
Organic Material ◽  
Organic Materials ◽  
Root Nodules ◽  
Block Design ◽  
Coconut Water ◽  
Randomized Block Design ◽  
Glycine Max L ◽  
Soybean Plants ◽  
Original Habitat

Azospirillium is a genus of non-symbiotic N fixer bacteria, known as a biological fertilizer inoculant. The quality of Azospirillum sp. inoculant often decrease when applied outside its original habitat. Therefore, it is necessary to maintain the viability and increase its effectiveness. One way that can be done is by adding organic material as additives as a source of energy for bacteria. Potential organic materials can be used as additives to stimulate the viability, activity and effectiveness of Azospirillium sp. are coconut water, molasses and bran. The organic material contained elements such as C, N and P as the source of nutrients for Azospirillium sp. which was inoculated in Soybean (Glycine max L.) plants. This experiment aimed to examine the effect of additive application on Azospirillum sp. population, root nodules, and seed weight of soybean plants (Glycine max L.) on Jatinangor Inceptisols. The experimental design used was Randomized Block Design (RBD) consisted of combination between of Azospirillum sp. and additives type, namely molasses, coconut water and bran. The results showed that the application of a mixture of bran, molasses, and coconut water attracted the population of Azospirillum sp. higher than the control treatments. Meanwhile molasses, coconut water, and additive mixtures have the potential to increase the number of root nodules and soybean pods yields. Coconut water, molasses and bran are organic materials that have the potential as additives that stimulate the activity and increase the population of Azospirillium sp. and soybean pods.

scholarly journals PERTUMBUHAN KEDELAI (Glycine max [L.] Merrill) VARIETAS ANJASMORO DENGAN PEMBERIAN BIOURIN KAMBING (Capra aegagrus hircus)

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Abd. Hamid ◽  
Riza Linda ◽  
Mukarlina Mukarlina
Keyword(s):  
Glycine Max ◽  
Root Nodules ◽  
Organic Fertilizer ◽  
West Kalimantan ◽  
Randomized Design ◽  
Capra Aegagrus ◽  
Glycine Max L ◽  
Number Of Leaves ◽  
Soybean Plants ◽  
Weight Number

Soybean (Glycine max [L.] Merrill) Anjasmoro variety is one of the superior soybean varieties widely cultivated in West Kalimantan. The growth of soybean can be increased by applying liquid organic fertilizer namely goat biourine. The purpose of this study was to determine the goat biourine quality and the effect of the application of goat biourine on the growth of Anjasmoro variety soybean plants. This study uses a Completely Randomized Design (CRD) consisting of five treatments, namely without biourine/control, 100 mL/L biourine, 200 mL/L biourine, 300 biourine mL/L and  400 mL/L biourine. Each treatment was repeated 5 times to obtain 25 experimental units. The analysis of goat biourine showed the value of C-Organic 2.03%, pH 4.83, N 0.72%, P 0.004% and K 0.234%. The results showed that apply of goat biourine had a significant effect on plant height, number of leaves, shoot wet of weight, shoot dry of weight, number of branches, number of root nodules and the number of effective root nodules, but it had not a significant effect on root wet of weight and root dry of weight.

Relation between nitrogenase activity and stem or xylem sap ureide content of soybean plants (Glycine max L. Merr)

Plant Science ◽  
1989 ◽  
Vol 61 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Christine Argillier ◽  
Jean-Jacques Drevon ◽  
Mathias Zengbe ◽  
Louis Salsac
Keyword(s):  
Glycine Max ◽  
Nitrogenase Activity ◽  
Xylem Sap ◽  
Glycine Max L ◽  
Soybean Plants

scholarly journals Elucidating the effects of TiO2 nanoparticles on the toxicity and accumulation of Cu in soybean plants (Glycine max L.)

2021 ◽  
Vol 219 ◽  
pp. 112312
Author(s):  
Yinlong Xiao ◽  
Ying Du ◽  
Yue Xiao ◽  
Xiaohong Zhang ◽  
Jun Wu ◽  
...  
Keyword(s):  
Glycine Max ◽  
Tio2 Nanoparticles ◽  
Glycine Max L ◽  
Soybean Plants

scholarly journals Quantitative Proteomics Reveals that GmENO2 Proteins Are Involved in Response to Phosphate Starvation in the Leaves of Glycine max L.

2021 ◽  
Vol 22 (2) ◽  
pp. 920
Author(s):  
Ling Cheng ◽  
Wanling Min ◽  
Man Li ◽  
Lili Zhou ◽  
Chuan-Chih Hsu ◽  
...  
Keyword(s):  
Glycine Max ◽  
Molecular Mechanisms ◽  
Human Life ◽  
Phosphate Starvation ◽  
Limiting Factor ◽  
Transcriptional Level ◽  
Label Free ◽  
Promoter Regions ◽  
Glycine Max L ◽  
Soybean Plants

Soybean (Glycine max L.) is a major crop providing important source for protein and oil for human life. Low phosphate (LP) availability is a critical limiting factor affecting soybean production. Soybean plants develop a series of strategies to adapt to phosphate (Pi) limitation condition. However, the underlying molecular mechanisms responsible for LP stress response remain largely unknown. Here, we performed a label-free quantification (LFQ) analysis of soybean leaves grown under low and high phosphate conditions. We identified 267 induced and 440 reduced differential proteins from phosphate-starved leaves. Almost a quarter of the LP decreased proteins are involved in translation processes, while the LP increased proteins are accumulated in chlorophyll biosynthetic and carbon metabolic processes. Among these induced proteins, an enolase protein, GmENO2a was found to be mostly induced protein. On the transcriptional level, GmENO2a and GmENO2b, but not GmENO2c or GmENO2d, were dramatically induced by phosphate starvation. Among 14 enolase genes, only GmENO2a and GmENO2b genes contain the P1BS motif in their promoter regions. Furthermore, GmENO2b was specifically induced in the GmPHR31 overexpressing soybean plants. Our findings provide molecular insights into how soybean plants tune basic carbon metabolic pathway to adapt to Pi deprivation through the ENO2 enzymes.

scholarly journals Aspectos Bionômicos de Spodoptera eridania (Cramer): Uma Praga em Expansão na Cultura da Soja na Região do Cerrado Brasileiro

2014 ◽  
Vol 7 (2) ◽  
pp. 75-80 ◽  
Author(s):  
Bruno Henrique Sardinha de Souza ◽  
Eduardo Neves Costa ◽  
Anderson Gonçalves da Silva ◽  
Arlindo Leal Boiça Júnior
Keyword(s):  
International Trade ◽  
Glycine Max ◽  
Insect Pests ◽  
High Yield ◽  
Spodoptera Eridania ◽  
Soybean Crop ◽  
Glycine Max L ◽  
Midwest Region ◽  
Soybean Plants ◽  
Economical Losses

A soja, Glycine max (L.) Merril, é uma das culturas de maior importância econômica para o Brasil, considerada uma commodity nacional devido à sua alta produtividade e participação nas exportações no mercado internacional. Dentre os insetos-pragas que causam danos para essa cultura, nos últimos anos agrícolas têm merecido destaque as lagartas de Spodoptera eridania (Cramer), as quais podem se alimentar tanto de folhas quanto das vagens das plantas de soja, causando prejuízos econômicos para os sojicultores, principalmente nas áreas do Cerrado localizadas na região Centro-Oeste do país. O objetivo da presente revisão é disponibilizar informações sobre os aspectos bionômicos de S. eridania, a fim de dar subsídios para futuras pesquisas sobre o manejo dessa praga.Bionomic Aspects of Spodoptera eridania (Cramer): A Pest in Expansion on Soybean Crop in the Region of Brazilian CerradoAbstract. Soybean, Glycine max (L.) Merril, represents one of the major economically important crops to Brazil, and is considered a national commodity because of its high yield and participation in international trade exportations. Among the insect pests that cause damage to this crop, Spodoptera eridania (Cramer) larvae highlighted in the last agricultural seasons by feeding on leaves and pods of soybean plants, and hence causing economical losses to soybean growers, especially in the Cerrado areas located in the Midwest region of the country. We aimed with this review to provide information about bionomical aspects of S. eridania in order to give subsides for further researches on the management of this pest.

scholarly journals Distribution of eggs, larvae and adults of Sternechus subsignatus Boheman on soybean plants in no-till system

1998 ◽  
Vol 27 (4) ◽  
pp. 513-518 ◽  
Author(s):  
Mauro T. B. Silva ◽  
Nelson Neto ◽  
Clara B. Hoffmann-Campo
Keyword(s):  
Glycine Max ◽  
Rio Grande ◽  
Sampling Time ◽  
Rio Grande Do Sul ◽  
No Till ◽  
Glycine Max L ◽  
Soybean Plants ◽  
Lateral Branches ◽  
Upper Third ◽  
Eggs And Larvae

Soybean [(Glycine max (L.) Merrill] commercial fields, maintained under a no-till system, were sampled during the crop seasons 1990/91 and 1996/97, in Cruz Alta and Júlio de Castilhos, Rio Grande do Sul State, to determine the distribution of eggs, larvae and adults of Sternechus subsignatus Boheman (Coleoptera : Curculionidae). Soybean plants and soil were examined at different time schedules. Eggs and larvae were recorded in main stems, lateral branches and leaf petioles, divided into the upper third, medium and lower third of soybean plants. Eggs (87%), and larvae (79%) were mainly observed in the main stem and in the medium plant sections (87% and 78%, respectively). Larval movement was not observed because larvae remained inside the galls, in the same area where the eggs were laid. Adults were located in different places depending on the sampling time. At night, adults were observed to move to the upper third of the plant and, during the day, down to the lower third of the plant and to the soil. The insects are normally concealed during their pre- and post- embryonic stage throughout most of the day.

Changes in phytohormone levels following inoculation of two soybean lines differing in nodulation

2002 ◽  
Vol 29 (8) ◽  
pp. 965 ◽  
Author(s):  
Asghari Bano ◽  
James E. Harper ◽  
Robert M. Auge ◽  
Dawn S. Neuman
Keyword(s):  
Glycine Max ◽  
Xylem Sap ◽  
Zeatin Riboside ◽  
Phloem Sap ◽  
Root Nodulation ◽  
Hormone Transport ◽  
Glycine Max L ◽  
Bradyrhizobium Inoculation

Changes in the concentration of free and conjugated ABA, zeatin riboside (ZR), and IAA in response to Bradyrhizobium inoculation and subsequent nodulation were monitored in xylem sap, phloem sap, and leaves of soybean [Glycine max (L.) Merr. cv. Williams 82] and its hypernodulating mutant, NOD1-3. In this study, pre-inoculation concentrations of phloem and xylem sap ABA and ZR were lower in NOD1-3 than in Williams 82, a difference that was accentuated in phloem after inoculation. The concentration of xylem ABA increased within 6�h of inoculation, while the concentration of phloem and leaf ABA did not change until 48-96 h after inoculation. Leaf uptake of [3H]ABA and distribution to phloem sap was greater in Williams 82 than in NOD1-3 during 48-72�h after inoculation. Inoculation resulted in similar increases in phloem and leaf IAA concentrations in both cultivars. While inoculation increased xylem sap ZR in both lines, the concentration of ZR increased much earlier in NOD1-3. Of particular interest is that ratios between hormones were altered during nodulation. Leaf and phloem ABA/IAA ratios were higher in Williams 82 than in the hypernod mutant, while the phloem IAA/ZR was greater from inoculation until nodulation in the NOD1-3 hypernod mutant. The xylem ABA/ZR ratio, as well as phloem ABA/ZR ratio, decreased in Williams 82 following inoculation, and leaf ABA concentration was elevated. The most noteworthy results of this study, therefore, came from an examination of the ratios between hormones in xylem and phloem sap, and the demonstration that hormone transport may play an important role in autoregulation of root nodulation.

Isotopic Discriminations During the Accumulation of Nitrogen by Soybeans

1988 ◽  
Vol 15 (3) ◽  
pp. 407 ◽  
Author(s):  
FJ Bergersen ◽  
MB Peoples ◽  
GL Turner
Keyword(s):  
N2 Fixation ◽  
Root Nodules ◽  
Xylem Sap ◽  
Vacuum Treatment ◽  
Natural Abundance ◽  
Mineral Nutrient ◽  
Similar Time ◽  
Total N ◽  
Plant Nitrogen ◽  
Nitrate Treatment

Soybeans were grown in a glasshouse in sand-vermiculite medium supplied daily with a mineral nutrient solution essentially free of combined N or containing 5 mM nitrate of known 15N abundance. The natural abundance of 15N in parts of plants and in nitrogen remaining in the medium was determined from 15 days after planting until fruiting. In nodulated plants completely dependent on N2 fixation for growth, the δ15N of plant nitrogen was uniformly negative at 56 days (overall mean: -0.90� 0.17) after adjustment for the effect of seed nitrogen. The δ15N of root nodules increased with time (max. 9.6‰), as that of shoots declined (min. - 1.3 ‰). The δ15N of every mainstem trifoliolate leaf and of the first (unifoliolate) leaf declined from initially positive values (0.5 to 2 ‰) to about - 2‰ with similar time courses, irrespective of the time of initiation. There were no significant losses of N from the plants during growth. There were differences between the δ15N of the total N of root-bleeding xylem sap and of sap extracted by vacuum treatment of stems. These were due to differences between the proportions of ureide-N and amino-N and between the δ15N values of these components. When nodulated plants were supplied daily with 5 mM nitrate (δ15N = 7.68‰) between 21 and 35 days, N2 fixation was reduced to 63% of N assimilated but growth and accumulation of nitrogen were affected little. Following removal of nitrate, there were changes in growth which led to enhanced nodulation and N2 fixation. The δ15N of the total N of trifoliolate leaves which were initiated or expanded before or during the period of nitrate treatment remained positive; those expanded or initiated after the treatment became negative in δ15N, as in the corresponding leaves of untreated nodulated plants. The δ15N of nodules was unaffected by the nitrate treatment. In plants (non-nod. Clark '63) supplied continuously with nitrate, the δ15N of the total N of entire plants rose quickly from values for seeds, but to values significantly higher than in the nitrate. These results are discussed in relation to the effects on the use of 15N natural abundance data for estimating utilisation of atmospheric N2 by nodulated plants.

Curled root hairs are a site of entry for Bradyrhizobium infecting hydroponically grown soybean plants with mature root systems

1988 ◽  
Vol 66 (4) ◽  
pp. 683-686 ◽  
Author(s):  
Jeanne M. L. Selker ◽  
John Imsande ◽  
Eldon H. Newcomb
Keyword(s):  
Glycine Max ◽  
Root Hair ◽  
Bradyrhizobium Japonicum ◽  
Root Hairs ◽  
Root Systems ◽  
Glycine Max L ◽  
Soybean Plants ◽  
A Site ◽  
Hydroponically Grown ◽  
Mature Root

Early emergent nodules on roots of hydroponically grown soybean plants (Glycine max (L.) Merr.) were sectioned serially to locate the site of infection by Bradyrhizobium japonicum. The plants had been inoculated only after their root systems had produced numerous higher order branches. The hydroponic solutions contained all required nutrients, including either a suboptimal concentration of nitrate (0.5 mM) or an excess of nitrate (4.0 mM). In all six nodules examined, three with suboptimal nitrate and three with excess nitrate, we found a centrally located root hair containing an infection thread. We conclude that mature root systems of soybean grown in aqueous culture can undergo infection through root hairs in the way that is typical of young seedlings grown either in pots of vermiculite or pouches.

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