scholarly journals Anti-Inflammatory Activity of Oat Beta-Glucans in a Crohn’s Disease Model: Time- and Molar Mass-Dependent Effects

2021 ◽  
Vol 22 (9) ◽  
pp. 4485
Author(s):  
Ewa Żyła ◽  
Katarzyna Dziendzikowska ◽  
Dariusz Kamola ◽  
Jacek Wilczak ◽  
Rafał Sapierzyński ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Signaling Pathways ◽  
Inflammatory Markers ◽  
Acute Inflammation ◽  
Molar Mass ◽  
Cytokine Signaling ◽  
Trinitrobenzene Sulfonic Acid ◽  
Beta Glucan ◽  
Beta Glucans

Background: The incidence of Crohn’s disease (CD) is increasing worldwide, and it has currently become a serious public health issue in society. The treatment of CD continues throughout a patient’s lifetime, and therefore, it is necessary to develop new, effective treatment methods, including dietotherapy. The present study aimed to determine the effects of consumption of oat beta-glucans with different molar mass on colon inflammation (colitis) in the early stages of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced CD in an animal model. Methods: Sprague–Dawley rats (control and TNBS-induced CD) were divided into three dietary groups and fed for 3 days (reflecting acute inflammation) or 7 days (reflecting remission) with a feed containing 1% low (βGl) or high (βGh) molar mass oat beta-glucan or a feed without this polysaccharide. The level of colon inflammatory markers and the expression of cytokines and their receptor genes were measured by ELISA and RT-PCR methods, respectively. Results: Acute inflammation or remission (3 or 7 days after TNBS administration, respectively) stages of experimentally induced CD were characterized by an increase in the level of inflammatory markers (IL-1, IL-6, IL-10, IL-12, TNF-α, CRP, MPO, COX, and PGE2) and the disruption of some cytokine signaling pathways as well as macro- and microscopic changes of colon tissue. The consumption of oat beta-glucans reduced the level of inflammatory markers and recovered the signaling pathways and histological changes, with stronger effects of βGl after 7 days of colitis. Conclusions: Dietary oat beta-glucans can reduce colitis at the molecular and organ level and accelerate CD remission.

scholarly journals Effects of Dietary Oat Beta-Glucans on Colon Apoptosis and Autophagy through TLRs and Dectin-1 Signaling Pathways—Crohn’s Disease Model Study

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 321
Author(s):  
Łukasz Kopiasz ◽  
Katarzyna Dziendzikowska ◽  
Małgorzata Gajewska ◽  
Michał Oczkowski ◽  
Kinga Majchrzak-Kuligowska ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Epithelial Cells ◽  
Caspase 3 ◽  
Disease Model ◽  
Time Dependent ◽  
Sprague Dawley ◽  
Beta Glucan ◽  
High Molar Mass ◽  
Beta Glucans

Background: Crohn’s disease (CD) is characterized by chronic inflammation of the gastrointestinal tract with alternating periods of exacerbation and remission. The aim of this study was to determine the time-dependent effects of dietary oat beta-glucans on colon apoptosis and autophagy in the CD rat model. Methods: A total of 150 Sprague–Dawley rats were divided into two main groups: healthy control (H) and a TNBS (2,4,6-trinitrobenzosulfonic acid)-induced colitis (C) group, both including subgroups fed with feed without beta-glucans (βG−) or feed supplemented with low- (βGl) or high-molar-mass oat beta-glucans (βGh) for 3, 7, or 21 days. The expression of autophagy (LC3B) and apoptosis (Caspase-3) markers, as well as Toll-like (TLRs) and Dectin-1 receptors, in the colon epithelial cells, was determined using immunohistochemistry and Western blot. Results: The results showed that in rats with colitis, after 3 days of induction of inflammation, the expression of Caspase-3 and LC3B in intestinal epithelial cells did not change, while that of TLR 4 and Dectin-1 decreased. Beta-glucan supplementation caused an increase in the expression of TLR 5 and Dectin-1 with no changes in the expression of Caspase-3 and LC3B. After 7 days, a high expression of Caspase-3 was observed in the colitis-induced animals without any changes in the expression of LC3B and TLRs, and simultaneously, a decrease in Dectin-1 expression was observed. The consumption of feed with βGl or βGh resulted in a decrease in Caspase-3 expression and an increase in TLR 5 expression in the CβGl group, with no change in the expression of LC3B and TLR 4. After 21 days, the expression of Caspase-3 and TLRs was not changed by colitis, while that of LC3B and Dectin-1 was decreased. Feed supplementation with βGh resulted in an increase in the expression of both Caspase-3 and LC3B, while the consumption of feed with βGh and βGl increased Dectin-1 expression. However, regardless of the type of nutritional intervention, the expression of TLRs did not change after 21 days. Conclusions: Dietary intake of βGl and βGh significantly reduced colitis by time-dependent modification of autophagy and apoptosis, with βGI exhibiting a stronger effect on apoptosis and βGh on autophagy. The mechanism of this action may be based on the activation of TLRs and Dectin-1 receptor and depends on the period of exacerbation or remission of CD.

Crohn's Disease Obstructions

2021 ◽  
Vol 34 (04) ◽  
pp. 227-232
Author(s):  
Molly M. Ford
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Acute Inflammation ◽  
Balloon Dilation ◽  
Additional Treatment ◽  
Endoscopic Balloon Dilation ◽  
Endoscopic Balloon

AbstractObstruction from stricturing Crohn's disease remains one of the most common reasons for intervention. Acute inflammation is often responsive to medications, but chronic fibrosis is unlikely to respond and will generally go on to require additional treatment. Newer methods, such as endoscopic balloon dilation, are gaining grounds in strictures that are amenable, but with high recurrence and strictures that may not be endoscopically accessible, surgery still plays a key role in the treatment of obstructing Crohn's disease.

Su1794 - Discrimination of Endoscopic Postoperative Recurrence in Patients with Crohn's Disease using Serological Inflammatory Markers

2018 ◽  
Vol 154 (6) ◽  
pp. S-586-S-587
Author(s):  
Kathleen Machiels ◽  
Magali de Bruyn ◽  
Sare Verstockt ◽  
Bram Verstockt ◽  
Gert A. Van Assche ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Inflammatory Markers ◽  
Postoperative Recurrence

Therapeutic effects of vasoactive intestinal peptide in the trinitrobenzene sulfonic acid mice model of Crohn's disease

2003 ◽  
Vol 124 (4) ◽  
pp. 961-971 ◽  
Author(s):  
Catalina Abad ◽  
Carmen Martinez ◽  
Maria G. Juarranz ◽  
Alicia Arranz ◽  
Javier Leceta ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Vasoactive Intestinal Peptide ◽  
Sulfonic Acid ◽  
Trinitrobenzene Sulfonic Acid ◽  
Therapeutic Effects ◽  
Mice Model

scholarly journals Time-Dependent Indirect Antioxidative Effects of Oat Beta-Glucans on Peripheral Blood Parameters in the Animal Model of Colon Inflammation

Antioxidants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 375 ◽  
Author(s):  
Łukasz Kopiasz ◽  
Katarzyna Dziendzikowska ◽  
Małgorzata Gajewska ◽  
Jacek Wilczak ◽  
Joanna Harasym ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Peripheral Blood ◽  
Molar Mass ◽  
Time Dependent ◽  
Mean Corpuscular Hemoglobin ◽  
Beta Glucan ◽  
High Molar Mass ◽  
Colon Inflammation ◽  
Beta Glucans

Background: Oat beta-glucans are polysaccharides, belonging to soluble fiber fraction, that show a wide spectrum of biological activity. The aim of this study was to evaluate the time-dependent antioxidative effect of chemically pure oat beta-glucan fractions, characterized by different molar mass, which were fed to animals with early stage of 2,4,6-trinitrobenzene sulfonic acid (TNBS) - induced colitis. Methods: The study was conducted on 150 adult male Sprague Dawley rats assigned to two groups—healthy control (H) and colitis (C) with colon inflammation induced by per rectum administration of TNBS. The animals from both groups were divided into 3 nutritional subgroups, receiving for 3, 7 or 21 days AIN-93M feed without beta-glucan (βG−) or with 1% (w/w) low molar mass oat beta-glucan (βGl+) or 1% (w/w) high molar mass oat beta-glucan (βGh+). After 3, 7 and 21 days, the animals were euthanized, peripheral blood was collected from the heart for further analysis. Results: The results of analyses performed on blood samples showed small changes in lymphocytes count and red blood cell parameters such as the number of red blood cell, mean corpuscular hemoglobin concentration and mean corpuscular volume (RBC, MCHC, MCV respectively) as well as normalization of antioxidant potential accompanying moderate inflammatory state of colon mucosa and submucosa. Conclusion: Oat beta-glucans exert an indirect antioxidant effect in animals with TNBS-induced colitis, with greater effectiveness in removing systemic effects of colon inflammation found for low molar mass oat beta-glucan.

scholarly journals Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease

2009 ◽  
Vol 206 (10) ◽  
pp. 2301-2301 ◽  
Author(s):  
Andrew M. Smith ◽  
Farooq Z. Rahman ◽  
Bu'Hussain Hayee ◽  
Simon J. Graham ◽  
Daniel J.B. Marks ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Acute Inflammation ◽  
Cytokine Secretion ◽  
Bacterial Clearance ◽  
Macrophage Cytokine

scholarly journals Small-Bowel Capsule Endoscopy in Patients with Suspected Crohn's Disease—Diagnostic Value and Complications

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Pedro Figueiredo ◽  
Nuno Almeida ◽  
Sandra Lopes ◽  
Gabriela Duque ◽  
Paulo Freire ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Small Bowel ◽  
Crohn's Disease ◽  
Capsule Endoscopy ◽  
Inflammatory Markers ◽  
Diagnostic Yield ◽  
Villous Atrophy ◽  
Tertiary Care ◽  
Endoscopic Findings ◽  
Capsule Retention

Background. The aim of this work was to assess the value of capsule enteroscopy in the diagnosis of patients with suspected Crohn's Disease (CD). Methods. This was a retrospective study in a single tertiary care centre involving patients undergoing capsule enteroscopy for suspected CD. Patients taking nonsteroidal anti inflammatory drugs during the thirty preceding days or with a follow-up period of less than six months were excluded. Results. Seventy eight patients were included. The endoscopic findings included mucosal breaks in 50%, ulcerated stenosis in 5%, and villous atrophy in 4%. The diagnosis of CD was established in 31 patients. The sensitivity, specificity, positive and negative predictive value of the endoscopic findings were 93%, 80%, 77%, and 94%, respectively. Capsule retention occurred in four patients (5%). The presence of ulcerated stenosis was significantly more frequent in patients with positive inflammatory markers. The diagnostic yield of capsule enteroscopy in patients with negative ileoscopy was 56%, with a diagnostic acuity of 93%. Conclusions. Small bowel capsule endoscopy is a safe and valid technique for assessing patients with suspected CD. Capsule retention is more frequent in patients with positive inflammatory markers. Patients with negative ileoscopy and suspected CD should be submitted to capsule enteroscopy.

Sign in / Sign up

Export Citation Format

Share Document