scholarly journals Transcriptome Analysis of Seed Weight Plasticity in Brassica napus

2021 ◽  
Vol 22 (9) ◽  
pp. 4449
Author(s):  
Javier Canales ◽  
José Verdejo ◽  
Gabriela Carrasco-Puga ◽  
Francisca M. Castillo ◽  
Anita Arenas-M ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Crop Yield ◽  
Stress Responses ◽  
Seed Weight ◽  
Rna Seq ◽  
Sucrose Transport ◽  
Factors Associated ◽  
Expression Of Genes ◽  
Source Sink ◽  
The Relationship

A critical barrier to improving crop yield is the trade-off between seed weight (SW) and seed number (SN), which has been commonly reported in several crops, including Brassica napus. Despite the agronomic relevance of this issue, the molecular factors involved in the interaction between SW and SN are largely unknown in crops. In this work, we performed a detailed transcriptomic analysis of 48 seed samples obtained from two rapeseed spring genotypes subjected to different source–sink (S–S) ratios in order to examine the relationship between SW and SN under different field conditions. A multifactorial analysis of the RNA-seq data was used to identify a group of 1014 genes exclusively regulated by the S–S ratio. We found that a reduction in the S–S ratio during seed filling induces the expression of genes involved in sucrose transport, seed weight, and stress responses. Moreover, we identified five co-expression modules that are positively correlated with SW and negatively correlated with SN. Interestingly, one of these modules was significantly enriched in transcription factors (TFs). Furthermore, our network analysis predicted several NAC TFs as major hubs underlying SW and SN compensation. Taken together, our study provides novel insights into the molecular factors associated with the SW–SN relationship in rapeseed and identifies TFs as potential targets when improving crop yield.

INFLUENCE OF IRRIGATION AND SEEDING RATES ON YIELD AND YIELD COMPONENTS OF BRASSICA NAPUS CV. TOWER

1978 ◽  
Vol 58 (3) ◽  
pp. 731-737 ◽  
Author(s):  
J. M. CLARKE ◽  
G. M. SIMPSON
Keyword(s):  
Brassica Napus ◽  
Yield Components ◽  
Seed Weight ◽  
Plant Morphology ◽  
Yield Component ◽  
Seeding Rate ◽  
Brassica Napus L ◽  
Relationship Of ◽  
The Relationship ◽  
Number Of Seeds

An analysis of the yield components of rape (Brassica napus L.) was carried out under field conditions for 2 yr at Saskatoon. Plant morphology and the crop environment were altered by the use of four planting densities and three water regimes. The number of branches per plant was scarcely affected by irrigation, but was reduced by high seeding rates. Number of pods per plant was increased by irrigation and reduced by high seeding rates. The number of seeds per pod was increased by irrigation, but reduced by high seeding rates. The 1,000-seed weight was increased both by irrigation and increased seeding rate, as was seed yield. Yield was positively correlated with 1,000-seed weight in both years. Yield component compensation was evident in the relationship of 1,000-seed weight to pod number and number of seeds per pod.

Growth and development of the pod wall in spring rape (Brassica napus) as related to the presence of seeds and exogenous phytohormones

1996 ◽  
Vol 127 (4) ◽  
pp. 487-500 ◽  
Author(s):  
Ancha Srinivasan ◽  
D. G. Morgan
Keyword(s):  
Brassica Napus ◽  
Seed Weight ◽  
Benzyl Adenine ◽  
Flower Opening ◽  
Delayed Senescence ◽  
Potted Plants ◽  
Pod Wall ◽  
The Relationship ◽  
Number Of Seeds ◽  
Spring Rape

SUMMARYPod wall photosynthesis is known to contribute to nearly one-third of rape (Brassica napus) seed weight. The relationship between the number of seeds and the growth of the pod wall, and the effects of single and mixed applications of gibberellic acid (GA), naphthyl acetic acid (NAA) and benzyl adenine (BA) on development of the pod wall were, therefore, examined in potted plants of spring rape under glasshouse conditions.The size and weight of the pod wall were closely associated with the number of seeds per pod, and a stronger development of tissues was observed in the presence of seeds. Application of BA alone or in combination with GA (GA + BA) to emasculated flowers induced the development of parthenocarpic pods similar in size and shape to the untreated seeded pods. NAA applied singly or in mixtures produced abnormal pods. BA enhanced cell division in all tissues while GA and NAA caused expansion of parenchyma in the mesocarp. Application of BA or GA + BA at 1–5 rather than at 11–15 days after flower opening led to the development of bigger pods. Younger pods responded more than the older ones when they were treated simultaneously. BA or GA + BA application to nonemasculated flowers on the terminal raceme delayed senescence in both basal and apical pods but it increased pod size only in the latter. The balance of cytokinins and gibberellins within a pod seems to play an important role in regulating the growth of the pod wall.

scholarly journals Brassica napus Infected with Leptosphaeria maculans

Genes ◽  
2019 ◽  
Vol 10 (4) ◽  
pp. 296 ◽  
Author(s):  
Ma ◽  
Wei ◽  
Lin ◽  
Zhang ◽  
Sun ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Stress Responses ◽  
Intron Retention ◽  
Leptosphaeria Maculans ◽  
Rna Seq ◽  
Hub Genes ◽  
Transcriptional Regulatory ◽  
Genes Encoding ◽  
Alternatively Spliced ◽  
Transcriptome Diversity

Alternative splicing (AS) is a post-transcriptional regulatory process that enhances transcriptome diversity, thereby affecting plant growth, development, and stress responses. To identify the new transcripts and changes in the isoform-level AS landscape of rapeseed (Brassica napus) infected with the fungal pathogen Leptosphaeria maculans, we compared eight RNA-seq libraries prepared from mock-inoculated and inoculated B. napus cotyledons and stems. The AS events that occurred in stems were almost the same as those in cotyledons, with intron retention representing the most common AS pattern. We identified 1892 differentially spliced genes between inoculated and uninoculated plants. We performed a weighted gene co-expression network analysis (WGCNA) to identify eight co-expression modules and their Hub genes, which are the genes most connected with other genes within each module. There are nine Hub genes, encoding nine transcription factors, which represent key regulators of each module, including members of the NAC, WRKY, TRAF, AP2/ERF-ERF, C2H2, C2C2-GATA, HMG, bHLH, and C2C2-CO-like families. Finally, 52 and 117 alternatively spliced genes in cotyledons and stems were also differentially expressed between mock-infected and infected materials, such as HMG and C2C2-Dof; which have dual regulatory mechanisms in response to L. maculans. The splicing of the candidate genes identified in this study could be exploited to improve resistance to L. maculans.

scholarly journals In Silico Identification of the Complex Interplay between Regulatory SNPs, Transcription Factors, and Their Related Genes in Brassica napus L. Using Multi-Omics Data

2021 ◽  
Vol 22 (2) ◽  
pp. 789
Author(s):  
Selina Klees ◽  
Thomas Martin Lange ◽  
Hendrik Bertram ◽  
Abirami Rajavel ◽  
Johanna-Sophie Schlüter ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Brassica Napus ◽  
Stress Responses ◽  
Oil Content ◽  
Regulation Of Gene Expression ◽  
Proteomics Data ◽  
Brassica Napus L ◽  
Essential Information ◽  
Expression Of Genes ◽  
Regulatory Snps

Regulatory SNPs (rSNPs) are a special class of SNPs which have a high potential to affect the phenotype due to their impact on DNA-binding of transcription factors (TFs). Thus, the knowledge about such rSNPs and TFs could provide essential information regarding different genetic programs, such as tissue development or environmental stress responses. In this study, we use a multi-omics approach by combining genomics, transcriptomics, and proteomics data of two different Brassica napus L. cultivars, namely Zhongshuang11 (ZS11) and Zhongyou821 (ZY821), with high and low oil content, respectively, to monitor the regulatory interplay between rSNPs, TFs and their corresponding genes in the tissues flower, leaf, stem, and root. By predicting the effect of rSNPs on TF-binding and by measuring their association with the cultivars, we identified a total of 41,117 rSNPs, of which 1141 are significantly associated with oil content. We revealed several enriched members of the TF families DOF, MYB, NAC, or TCP, which are important for directing transcriptional programs regulating differential expression of genes within the tissues. In this work, we provide the first genome-wide collection of rSNPs for B. napus and their impact on the regulation of gene expression in vegetative and floral tissues, which will be highly valuable for future studies on rSNPs and gene regulation.

scholarly journals Molecular fingerprint of female bovine embryos produced in vitro with high competence to establish and maintain pregnancy†

2019 ◽  
Vol 102 (2) ◽  
pp. 292-305 ◽  
Author(s):  
A M Zolini ◽  
J Block ◽  
M B Rabaglino ◽  
P Tríbulo ◽  
M Hoelker ◽  
...  
Keyword(s):  
Cellular Responses ◽  
Rna Seq ◽  
Bovine Embryos ◽  
Molecular Fingerprint ◽  
Expression Of Genes ◽  
Artificial Environment ◽  
High Competence ◽  
The Relationship ◽  
Stimulating Factor

Abstract The objective was to identify the transcriptomic profile of in vitro-derived embryos with high competence to establish and maintain gestation. Embryos produced with X-sorted sperm were cultured from day 5 to day 7 in serum-free medium containing 10 ng/ml recombinant bovine colony-stimulating factor 2 (CSF2) or vehicle. The CSF2 was administered because this molecule can increase blastocyst competence for survival after embryo transfer. Blastocysts were harvested on day 7 of culture and manually bisected. One demi-embryo from a single blastocyst was transferred into a synchronized recipient and the other half was used for RNA-seq analysis. Using P < 0.01 and a fold change >2-fold or <0.5 fold as cutoffs, there were 617 differentially expressed genes (DEG) between embryos that survived to day 30 of gestation vs those that did not, 470 DEG between embryos that survived to day 60 and those that did not, 432 DEG between embryos that maintained pregnancy from day 30 to day 60 vs those where pregnancy failed after day 30, and 635 DEG regulated by CSF2. Pathways and ontologies in which DEG were overrepresented included many related to cellular responses to stress and cell survival. It was concluded that gene expression in the blastocyst is different between embryos that are competent to establish and maintain pregnancy vs those that are not. The relationship between expression of genes related to cell stress and subsequent embryonic survival probably reflects cellular perturbations caused by embryonic development taking place in the artificial environment associated with cell culture.

scholarly journals Changes in seed weight in response to different sources: sink ratio in oilseed rape

2014 ◽  
Vol 4 (1) ◽  
pp. 44-52 ◽  
Author(s):  
Francisco M Iglesias ◽  
Daniel J Miralles
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Yield Components ◽  
Seed Weight ◽  
Field Conditions ◽  
Brassica Napus L ◽  
Seed Filling ◽  
Incoming Radiation ◽  
Source Sink ◽  
Different Sources

Little knowledge exists about the degree of source, sink and source: sink limitations on mean seed weight in oilseed rape (Brassica napus L.). The objective of this work was to analyze the nature and magnitude on seed weight response to assimilate availability during the effective seed-filling period in oilseed rape. Three Argentinean varieties, Eclipse, Impulse, and Master, were grown under field conditions, and at the beginning of the effective seed filling period, a broad range of source: sink manipulation combinations were produced. Source manipulations consisted of two incoming radiation (R) level reductions: 0% (Rn) and ~50% (Rs) combined with three different sources: sink treatments were applied: C, control; PR, ~50% pod removal, and D, 100% defoliation. Rs significantly reduced yield (15%) and MSW (12%) with respect to Rn, without significant effects on the rest of the sub yield components. Source:sink manipulation treatments significantly affected all yield components. PR diminished yield by 29%, reducing ca. 40% seeds pl-1 by reductions pods pl-1 (41%) with respect to Rn, whereas PR increased MSW by 19%, counterbalancing the reduction in seeds pl-1 and thereby in yield. When considering different seed positions along the main raceme, Rs reduced MSW by 12% independently of seed positions onto the raceme. On the contrary, PR increased MSW in average 17% with respect to C. Results reported here suggest that oilseed rape has source: sink co-limitation during the effective seed filling period, which is apparently higher than wheat and lower than maize. DOI: http://dx.doi.org/10.3329/ijarit.v4i1.21091 Int. J. Agril. Res. Innov. & Tech. 4 (1): 44-52, June, 2014

K-MEANS CLUSTERING ALGORITHM BASED CLASSIFICATION OF SOIL FERTILITY IN NORTH WEST NIGERIA

2020 ◽  
Vol 4 (2) ◽  
pp. 780-787
Author(s):  
Ibrahim Hassan Hayatu ◽  
Abdullahi Mohammed ◽  
Barroon Ahmad Isma’eel ◽  
Sahabi Yusuf Ali
Keyword(s):  
Soil Fertility ◽  
Crop Yield ◽  
Clustering Algorithm ◽  
Soil Samples ◽  
North West ◽  
R Programming ◽  
Available Information ◽  
Northwest Region ◽  
The Relationship

Soil fertility determines a plant's development process that guarantees food sufficiency and the security of lives and properties through bumper harvests. The fertility of soil varies according to regions, thereby determining the type of crops to be planted. However, there is no repository or any source of information about the fertility of the soil in any region in Nigeria especially the Northwest of the country. The only available information is soil samples with their attributes which gives little or no information to the average farmer. This has affected crop yield in all the regions, more particularly the Northwest region, thus resulting in lower food production.  Therefore, this study is aimed at classifying soil data based on their fertility in the Northwest region of Nigeria using R programming. Data were obtained from the department of soil science from Ahmadu Bello University, Zaria. The data contain 400 soil samples containing 13 attributes. The relationship between soil attributes was observed based on the data. K-means clustering algorithm was employed in analyzing soil fertility clusters. Four clusters were identified with cluster 1 having the highest fertility, followed by 2 and the fertility decreases with an increasing number of clusters. The identification of the most fertile clusters will guide farmers on where best to concentrate on when planting their crops in order to improve productivity and crop yield.

Empathy as moderator of gender differences in Chinese insurance agents' business ethical sensitivity

2017 ◽  
Vol 45 (6) ◽  
pp. 1029-1042 ◽  
Author(s):  
Na Zhang ◽  
Jian Zhang ◽  
Jing Wang
Keyword(s):  
Gender Differences ◽  
Business Ethics ◽  
Research Field ◽  
Ethical Sensitivity ◽  
Men And Women ◽  
Insurance Agents ◽  
Factors Associated ◽  
Ethics Research ◽  
The Relationship ◽  
Insight Into

To expand the business ethics research field, and to increase society's understanding of Chinese insurance agents' business ethics, we investigated how gender differences are related to agents' business ethical sensitivity and whether or not these relationships are moderated by empathy. Through a regression analysis of the factors associated with the business ethical sensitivity of 417 Chinese insurance agents, we found that gender played an important role in affecting business ethical sensitivity, and empathy significantly affected business ethical sensitivity. Furthermore, empathy had a moderating effect on the relationship between gender and business ethical sensitivity. Both men and women with strong empathy scored high on business ethical sensitivity; however, men with strong empathy had higher levels of business ethical sensitivity than did women with little empathy. The findings add to the literature by providing insight into the mechanisms responsible for the benefits of empathy in increasing business ethical sensitivity.

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