scholarly journals Metabolite Analysis of Jerusalem Artichoke (Helianthus tuberosus L.) Seedlings in Response to Polyethylene Glycol-Simulated Drought Stress

2021 ◽  
Vol 22 (7) ◽  
pp. 3294
Author(s):  
Mengliang Zhao ◽  
Yanjing Ren ◽  
Wei Wei ◽  
Jiaming Yang ◽  
Qiwen Zhong ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Drought Stress ◽  
Fatty Acid Biosynthesis ◽  
Jerusalem Artichoke ◽  
Ultra Performance Liquid Chromatography ◽  
Raw Material ◽  
Perennial Crop ◽  
Phenolic Metabolism ◽  
Resistant Varieties ◽  
Simulated Drought

Jerusalem artichokes are a perennial crop with high drought tolerance and high value as a raw material to produce biofuels, functional feed, and food. However, there are few comprehensive metabolomic studies on Jerusalem artichokes under drought conditions. Methods: Ultra-performance liquid chromatography and tandem mass spectrometry were used to identify differential metabolites in Jerusalem artichoke seedling leaves under polyethylene glycol (PEG) 6000-simulated drought stress at 0, 18, 24, and 36 h. Results: A total of 661 metabolites and 236 differential metabolites were identified at 0 vs. 18, 18 vs. 24, and 24 vs. 36 h. 146 differential metabolites and 56 common were identified and at 0 vs. 18, 24, and 36 h. Kyoto Encyclopedia of Genes and Genomes enrichment identified 236 differential metabolites involved in the biosynthesis of secondary metabolites and amino acids. Metabolites involved in glycolysis, phenolic metabolism, tricarboxylic cycle, glutamate-mediated proline biosynthesis, urea cycle, amino acid metabolism, unsaturated fatty acid biosynthesis, and the met salvage pathway responded to drought stress. Conclusion: A metabolic network in the leaves of Jerusalem artichokes under drought stress is proposed. These results will improve understanding of the metabolite response to drought stress in Jerusalem artichokes and develop a foundation for breeding drought-resistant varieties.

Endogenous Salicylic Acid Levels and Signaling Positively Regulate Arabidopsis Response to Polyethylene Glycol-Simulated Drought Stress

2014 ◽  
Vol 33 (4) ◽  
pp. 871-880 ◽  
Author(s):  
Qingqing He ◽  
Shiyang Zhao ◽  
Qunfei Ma ◽  
Yiyan Zhang ◽  
Linli Huang ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Polyethylene Glycol ◽  
Drought Stress ◽  
Simulated Drought

scholarly journals Mucilage inhibits germination of desert ephemeral Nepeta micrantha seed under moderate osmotic stress and promotes recovery after release of this stress

2020 ◽  
Vol 48 (1) ◽  
pp. 21-25
Author(s):  
Chunxiang Zhao ◽  
Li Jiang ◽  
Xiang Shi ◽  
Lei Wang
Keyword(s):  
Polyethylene Glycol ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Osmotic Stress ◽  
Outer Surface ◽  
Temperature Conditions ◽  
Ephemeral Plant ◽  
Simulated Drought ◽  
Recovery Percentage

Mucilage is considered to play an important role in the survival of seeds in harsh desert environments. Nepeta micrantha is an ephemeral plant of the Gurbantungut Desert, China. The outer surface of N. micrantha nutlets contains a layer of mucilage. We hypothesised that mucilage improves germination during and after osmotic stress. Germination of both intact and demucilaged nutlets under different polyethylene glycol (PEG)-simulated drought stress was tested at the optimal light and temperature conditions. Germination of intact and demucilaged nutlets decreased with an increase in PEG concentration. However, there were no significant differences in germination between intact and demucilaged nutlets at the same PEG concentration, except at 15% PEG. Recovery percentage and final germination of intact nutlets were considerably higher than that of demucilaged nutlets after treatment with 15% PEG. These findings demonstrate that mucilage contributes significantly to drought tolerance ofN. micrantha nutlets by decreasing germination under moderate osmotic stress and aiding germination after the release of this stress.

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