scholarly journals Tailored-Made Polydopamine Nanoparticles to Induce Ferroptosis in Breast Cancer Cells in Combination with Chemotherapy

2021 ◽  
Vol 22 (6) ◽  
pp. 3161
Author(s):  
Celia Nieto ◽  
Milena A. Vega ◽  
Eva M. Martín del Valle
Keyword(s):  
Breast Cancer ◽  
Antitumor Activity ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Breast Tumors ◽  
In Vitro Assays ◽  
Acidic Ph ◽  
Therapeutic Activity ◽  
Ph Values

Ferroptosis is gaining followers as mechanism of selective killing cancer cells in a non-apoptotic manner, and novel nanosystems capable of inducing this iron-dependent death are being increasingly developed. Among them, polydopamine nanoparticles (PDA NPs) are arousing interest, since they have great capability of chelating iron. In this work, PDA NPs were loaded with Fe3+ at different pH values to assess the importance that the pH may have in determining their therapeutic activity and selectivity. In addition, doxorubicin was also loaded to the nanoparticles to achieve a synergist effect. The in vitro assays that were performed with the BT474 and HS5 cell lines showed that, when Fe3+ was adsorbed in PDA NPs at pH values close to which Fe(OH)3 begins to be formed, these nanoparticles had greater antitumor activity and selectivity despite having chelated a smaller amount of Fe3+. Otherwise, it was demonstrated that Fe3+ could be released in the late endo/lysosomes thanks to their acidic pH and their Ca2+ content, and that when Fe3+ was co-transported with doxorubicin, the therapeutic activity of PDA NPs was enhanced. Thus, reported PDA NPs loaded with both Fe3+ and doxorubicin may constitute a good approach to target breast tumors.

The in vitro therapeutic activity of betulinic acid nanocomposite on breast cancer cells (MCF-7) and normal fibroblast cell (3T3)

2014 ◽  
Vol 49 (23) ◽  
pp. 8171-8182 ◽  
Author(s):  
Samer Hasan Hussein-Al-Ali ◽  
Palanisamy Arulselvan ◽  
Sharida Fakurazi ◽  
Mohd Zobir Hussein
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Betulinic Acid ◽  
Breast Cancer Cells ◽  
Fibroblast Cell ◽  
Normal Fibroblast ◽  
Therapeutic Activity ◽  
Mcf 7

Novel merosesquiterpene exerts a potent antitumor activity against breast cancer cells in vitro and in vivo

2014 ◽  
Vol 79 ◽  
pp. 1-12 ◽  
Author(s):  
Esther Carrasco ◽  
Pablo Juan Álvarez ◽  
Consolación Melguizo ◽  
José Prados ◽  
Enrique Álvarez-Manzaneda ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Antitumor Activity ◽  
Cancer Cells ◽  
Breast Cancer Cells

scholarly journals Zerumbone Induces Apoptosis in Breast Cancer Cells by Targeting αvβ3 Integrin upon Co-Administration with TP5-iRGD Peptide

Molecules ◽  
2019 ◽  
Vol 24 (14) ◽  
pp. 2554 ◽  
Author(s):  
Eltayeb E. M. Eid ◽  
Abdulrahman S. Alanazi ◽  
Sanaz Koosha ◽  
Alian A. Alrasheedy ◽  
Faizul Azam ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Computational Modelling ◽  
Cell Penetrating Peptides ◽  
In Vitro Assays ◽  
Αvβ3 Integrin ◽  
Cell Viability Assay ◽  
Mcf 7

Cell-penetrating peptides (CPPs) are highly promising tools to deliver therapeutic molecules into tumours. αVβ3 integrins are cell–matrix adhesion receptors, and are considered as an attractive target for anticancer therapies owing to their roles in the process of metastasis and angiogenesis. Therefore, this study aims to assess the effect of co-administration of zerumbone (ZER) and ZERencapsulated in hydroxypropyl-β-cyclodextrin with TP5-iRGD peptide towards cell cytotoxicity, apoptosis induction, and proliferation of normal and cancerous breast cells utilizing in vitro assays, as well as to study the molecular docking of ZER in complex with TP5-iRGD peptide. Cell viability assay findings indicated that ZER and ZERencapsulated in hydroxypropyl-β-cyclodextrin (ZER-HPβCD) inhibited the growth of estrogen receptor positivebreast cancer cells (ER+ MCF-7) at 72 h treatment with an inhibitory concentration (IC)50 of 7.51 ± 0.2 and 5.08 ± 0.2 µg/mL, respectively, and inhibited the growth of triple negative breast cancer cells (MDA-MB-231) with an IC50 of 14.96 ± 1.52 µg/mL and 12.18 ± 0.7 µg/mL, respectively. On the other hand, TP5-iRGD peptide showed no significant cytotoxicity on both cancer and normal cells. Interestingly, co-administration of TP5-iRGD peptide in MCF-7 cells reduced the IC50 of ZER from 7.51 ± 0.2 µg/mL to 3.13 ± 0.7 µg/mL and reduced the IC50 of ZER-HPβCD from 5.08 ± 0.2 µg/mL to 0.49 ± 0.004 µg/mL, indicating that the co-administration enhances the potency and increases the efficacy of ZER and ZER-HPβCD compounds. Acridine orange (AO)/propidium iodide (PI) staining under fluorescence microscopy showed evidence of early apoptosis after 72 h from the co-administration of ZER or ZER-HPβCD with TP5-iRGD peptide in MCF-7 breast cancer cells. The findings of the computational modelling experiment provide novel insights into the ZER interaction with integrin αvβ3 in the presence of TP5-iRGD, and this could explain why ZER has better antitumor activities when co-administered with TP5-iRGD peptide.

The In Vitro Therapeutic Activity of Ellagic Acid-Alginate-Silver Nanoparticles on Breast Cancer Cells (MCF-7) and Normal Fibroblast Cells (3T3)

2016 ◽  
Vol 8 (3) ◽  
pp. 545-553 ◽  
Author(s):  
Samer Hasan Hussein-Al-Ali ◽  
Sepideh Keshan Balavandy ◽  
Zurina Zainal Abidin ◽  
Aminu Umar Kura ◽  
Sharida Fakurazi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Ellagic Acid ◽  
Breast Cancer Cells ◽  
Normal Fibroblast ◽  
Fibroblast Cells ◽  
Therapeutic Activity ◽  
Mcf 7

Dinuclear CuIcomplexes of pyridyl-diazadiphosphetidines and aminobis(phosphonite) ligands: synthesis, structural studies and antiproliferative activity towards human cervical, colon carcinoma and breast cancer cells

2014 ◽  
Vol 43 (29) ◽  
pp. 11339-11351 ◽  
Author(s):  
Aijaz Rashid ◽  
Guddekoppa S. Ananthnag ◽  
Susmita Naik ◽  
Joel T. Mague ◽  
Dulal Panda ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Antitumor Activity ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Antiproliferative Activity ◽  
Breast Cancer Cells ◽  
Human Tumor ◽  
Structural Studies ◽  
Human Tumor Cells

The CuIcomplexes showedin vitroantitumor activity against several human tumor cells 5–7 fold higher than cisplatin.

N-hexane Extract and Fraction of Green Tea as Antiproliferation of MCM-B2 Breast Cancer Cells In Vitro

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Lisni Noraida Waruwu ◽  
Maria Bintang ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Green Tea ◽  
Cancer Cell ◽  
Breast Cancer Cells ◽  
Green Tea Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Tea Extract

Green tea (Camellia sinensis) is one of traditional plants that have the potential as an anticancer. The sample used in this research commercial green tea extract. The purpose of this study was to test the antiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract fractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and fraction of green tea water have value Lethality Concentration 50 (LC50) more than 1000 ppm. The fraction of ethanol 70% and n-hexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of the phytochemical screening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screening results of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancer cells MCM-B2 and normal cells Vero by trypan blue staining method. The highest MCM-B2 cell inhibitory activity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction, 59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Vero cells characterized by not inhibiting normal cell proliferation. Keywords: antiproliferative, cancer cell MCM-B2, commercial green tea, cytotoxicity

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