scholarly journals The Effects of Cold Atmospheric Pressure Plasma on Germination Parameters, Enzyme Activities and Induction of DNA Damage in Barley

2021 ◽  
Vol 22 (6) ◽  
pp. 2833
Author(s):  
Mária Peťková ◽  
Renáta Švubová ◽  
Stanislav Kyzek ◽  
Veronika Medvecká ◽  
Ľudmila Slováková ◽  
...  
Keyword(s):  
Dna Damage ◽  
Enzyme Activities ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Agricultural Practice ◽  
Plasma Exposure ◽  
Positive Effects ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma ◽  
Germination Parameters

Climate change, environmental pollution and pathogen resistance to available chemical agents are part of the problems that the food industry has to face in order to ensure healthy food for people and livestock. One of the promising solutions to these problems is the use of cold atmospheric pressure plasma (CAPP). Plasma is suitable for efficient surface decontamination of seeds and food products, germination enhancement and obtaining higher yields in agricultural production. However, the plasma effects vary due to plasma source, treatment conditions and seed type. In our study, we tried to find the proper conditions for treatment of barley grains by diffuse coplanar surface barrier discharge, in which positive effects of CAPP, such as enhanced germination or decontamination effects, would be maximized and harmful effects, such as oxidation and genotoxic potential, minimized. Besides germination parameters, we evaluated DNA damage and activities of various germination and antioxidant enzymes in barley seedlings. Plasma exposure resulted in changes in germination parameters and enzyme activities. Longer exposures had also genotoxic effects. As such, our findings indicate that appropriate plasma exposure conditions need to be carefully optimized in order to preserve germination, oxidation balance and genome stability, should CAPP be used in agricultural practice.

scholarly journals Cold Atmospheric Pressure Plasma Treatment of Maize Grains—Induction of Growth, Enzyme Activities and Heat Shock Proteins

2021 ◽  
Vol 22 (16) ◽  
pp. 8509
Author(s):  
Ľudmila Holubová ◽  
Renáta Švubová ◽  
Ľudmila Slováková ◽  
Boris Bokor ◽  
Valéria Chobotová Kročková ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Enzyme Activities ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Lytic Enzyme ◽  
Prolonged Treatment ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma ◽  
Shock Proteins

Zea mays L. is one of the most produced crops, and there are still parts of the world where maize is the basic staple food. To improve agriculture, mankind always looks for new, better methods of growing crops, especially in the current changing climatic conditions. Cold atmospheric pressure plasma (CAPP) has already showed its potential to enhance the culturing of crops, but it still needs more research for safe implementation into agriculture. In this work, it was shown that short CAPP treatment of maize grains had a positive effect on the vitality of grains and young seedlings, which may be connected to stimulation of antioxidant and lytic enzyme activities by short CAPP treatment. However, the prolonged treatment had a negative impact on the germination, growth, and production indexes. CAPP treatment caused the increased expression of genes for heat shock proteins HSP101 and HSP70 in the first two days after sowing. Using comet assay it was observed that shorter treatment times (30–120 s) did not cause DNA damage. Surface diagnostics of plasma-treated grains showed that plasma increases the hydrophilicity of the surface but does not damage the chemical bonds on the surface.

scholarly journals Oxidative modification and electrochemical inactivation of Escherichia coli upon cold atmospheric pressure plasma exposure

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0173618 ◽  
Author(s):  
Marlène Dezest ◽  
Anne-Laure Bulteau ◽  
Damien Quinton ◽  
Laurent Chavatte ◽  
Mickael Le Bechec ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Oxidative Modification ◽  
Plasma Exposure ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma

scholarly journals On cold atmospheric-pressure plasma jet induced DNA damage in cells

2020 ◽  
Vol 54 (3) ◽  
pp. 035203
Author(s):  
Nishtha Gaur ◽  
Hirofumi Kurita ◽  
Jun-Seok Oh ◽  
Saki Miyachika ◽  
Masafumi Ito ◽  
...  
Keyword(s):  
Dna Damage ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Atmospheric Pressure Plasma Jet ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma ◽  
In Cells

scholarly journals Evaluation of the Impact of Cold Atmospheric Pressure Plasma on Soybean Seed Germination

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 177
Author(s):  
Renáta Švubová ◽  
Ľudmila Slováková ◽  
Ľudmila Holubová ◽  
Dominika Rovňanová ◽  
Eliška Gálová ◽  
...  
Keyword(s):  
Dna Damage ◽  
Seed Germination ◽  
Succinate Dehydrogenase ◽  
Atmospheric Pressure ◽  
Soybean Seed ◽  
Atmospheric Pressure Plasma ◽  
Ambient Air ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma ◽  
The Impact

The present study aims to define the effects of Cold Atmospheric Pressure Plasma (CAPP) exposure on seed germination of an agriculturally important crop, soybean. Seed treatment with lower doses of CAPP generated in ambient air and oxygen significantly increased the activity of succinate dehydrogenase (Krebs cycle enzyme), proving the switching of the germinating seed metabolism from anoxygenic to oxygenic. In these treatments, a positive effect on seed germination was documented (the percentage of germination increased by almost 20% compared to the untreated control), while the seed and seedling vigour was also positively affected. On the other hand, higher exposure times of CAPP generated in a nitrogen atmosphere significantly inhibited succinate dehydrogenase activity, but stimulated lactate and alcohol dehydrogenase activities, suggesting anoxygenic metabolism. It was also found that plasma exposure caused a slight increment in the level of primary DNA damage in ambient air- and oxygen-CAPP treatments, and more significant DNA damage was found in nitrogen-CAPP treatments. Although a higher level of DNA damage was also detected in the negative control (untreated seeds), this might be associated with the age of seeds followed by their lower germination capacity (with the germination percentage reaching only about 60%).

Fabrication and Performance of a Multidischarge Cold-Atmospheric Pressure Plasma Array

2021 ◽  
pp. 1-8
Author(s):  
Kenneth A. Cornell ◽  
Amanda White ◽  
Adam Croteau ◽  
Jessica Carlson ◽  
Zeke Kennedy ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma ◽  
And Performance

scholarly journals Cold atmospheric pressure plasma jets as sources of singlet delta oxygen for biomedical applications

2011 ◽  
Vol 109 (12) ◽  
pp. 123302 ◽  
Author(s):  
J. S. Sousa ◽  
K. Niemi ◽  
L. J. Cox ◽  
Q. Th. Algwari ◽  
T. Gans ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Biomedical Applications ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jets ◽  
Pressure Plasma ◽  
Cold Atmospheric Pressure Plasma
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