scholarly journals Salinity Effects on Guard Cell Proteome in Chenopodium quinoa

2021 ◽  
Vol 22 (1) ◽  
pp. 428
Author(s):  
Fatemeh Rasouli ◽  
Ali Kiani-Pouya ◽  
Lana Shabala ◽  
Leiting Li ◽  
Ayesha Tahir ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Salinity Stress ◽  
Chenopodium Quinoa ◽  
Aspartic Proteinase ◽  
Stomatal Aperture ◽  
Salinity Treatment ◽  
Tryptophan Synthase ◽  
Ontology Term ◽  
Triacylglycerol Lipase ◽  
Exogenous Application

Epidermal fragments enriched in guard cells (GCs) were isolated from the halophyte quinoa (Chenopodium quinoa Wild.) species, and the response at the proteome level was studied after salinity treatment of 300 mM NaCl for 3 weeks. In total, 2147 proteins were identified, of which 36% were differentially expressed in response to salinity stress in GCs. Up and downregulated proteins included signaling molecules, enzyme modulators, transcription factors and oxidoreductases. The most abundant proteins induced by salt treatment were desiccation-responsive protein 29B (50-fold), osmotin-like protein OSML13 (13-fold), polycystin-1, lipoxygenase, alpha-toxin, and triacylglycerol lipase (PLAT) domain-containing protein 3-like (eight-fold), and dehydrin early responsive to dehydration (ERD14) (eight-fold). Ten proteins related to the gene ontology term “response to ABA” were upregulated in quinoa GC; this included aspartic protease, phospholipase D and plastid-lipid-associated protein. Additionally, seven proteins in the sucrose–starch pathway were upregulated in the GC in response to salinity stress, and accumulation of tryptophan synthase and L-methionine synthase (enzymes involved in the amino acid biosynthesis) was observed. Exogenous application of sucrose and tryptophan, L-methionine resulted in reduction in stomatal aperture and conductance, which could be advantageous for plants under salt stress. Eight aspartic proteinase proteins were highly upregulated in GCs of quinoa, and exogenous application of pepstatin A (an inhibitor of aspartic proteinase) was accompanied by higher oxidative stress and extremely low stomatal aperture and conductance, suggesting a possible role of aspartic proteinase in mitigating oxidative stress induced by saline conditions.

scholarly journals Exogenous Spermine Mitigate Adversities of Salinity Induced Oxidative Stress through Antioxidant Metabolites in Wheat

2021 ◽  
pp. 77-93
Author(s):  
Midathala Raghavendra ◽  
Dommalapati Sudhakara Rao ◽  
Naresh Kumar ◽  
Shashi Madan ◽  
Renu Munjal
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Crop Production ◽  
Ascorbic Acid Content ◽  
Carotenoid Content ◽  
Salinity Treatment ◽  
Wheat Varieties ◽  
Exogenous Application ◽  
Antioxidant Metabolites ◽  
Mda Content

Change in climatic scenarios due to global warming is characterized by extreme climate variability, land and water degradation which resulted in water scarcity. Accumulation of salts at the surface and sub-surface layers of soils affect crop production of major cereals which is a constraint in sustainable food production. Salinity is a major challenge to tackle wheat cultivation and harness productivity in arid and semi-arid regions of India. In the present investigation, mitigation of salinity induced oxidative stress through exogenous application of spermine (Spm) in four wheat genotypes was studied in relation to antioxidant metabolites. The levels of O2.- increased with increasing levels of salinity in wheat flag leaves. DBW 88 showed the levels of O2.- of 11.75 nmol g-1 FW and 15.74 nmol g-1 FW (at 8 dSm-1 and 12 dSm-1 respectively) at 21 Days After Sowing (DAS) and application of Spm decreased the O2.- content under control and saline stressed conditions at 8 dSm-1 and 12 dSm-1. Hydrogen peroxide content was increased with increasing levels of salinity in all the wheat varieties at 21 DAS. However, the increase was more in the case of DBW 88 when compared with HD 3086. Treatment of Spm decreased the H2O2 content when compared with control and saline stressed wheat varieties. The malondialdehyde (MDA) content was increased with increasing levels of salinity at 21 DAS. The highest increase in MDA content was seen in DBW 88 whereas the lowest increase was found in Kharchia 65. Application of Spm decreased the MDA content under control at both levels of salinity treated wheat varieties. The carotenoid content decreased with increasing levels of salinity in all four wheat varieties. However, the decrease was more in DBW 88 when compared with other varieties viz. HD 3086, Kharchia 65 and KRL 210 at 21 DAS. Exogenous Spm increased the carotenoids content in all four wheat varieties irrespective of the salinity. The leaves of Kharchia 65 and KRL 210 had higher levels of ascorbic acid as compared to that of DBW 88 and HD 3086. Increased content of carotenoid was observed in Spm-treated wheat. Exogenous application of Spm increased the ascorbic acid content in control at both levels of salt stress. The glutathione content increased with an increase in salinity treatment in all the varieties however, a higher increase was observed in Kharchia 65. Exogenous Spm increased the glutathione content in all the varieties irrespective of salinity stress. The results presented in the study indicated that the exogenous application of Spm improved their tolerance levels under salinity.

scholarly journals Exogenous Myo-inositol Alleviates Salt Stress by Enhancing Antioxidants and Membrane Stability via the Upregulation of Stress Responsive Genes in Chenopodium quinoa L.

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2416
Author(s):  
Amina A. M. Al-Mushhin ◽  
Sameer H. Qari ◽  
Marwa A. Fakhr ◽  
Ghalia S. H. Alnusairi ◽  
Taghreed S. Alnusaire ◽  
...  
Keyword(s):  
Salt Stress ◽  
Oxidative Damage ◽  
Chlorophyll Content ◽  
Salinity Stress ◽  
Foliar Application ◽  
Soluble Sugars ◽  
Chenopodium Quinoa ◽  
Membrane Stability ◽  
Central Position ◽  
Exogenous Application

Myo-inositol has gained a central position in plants due to its vital role in physiology and biochemistry. This experimental work assessed the effects of salinity stress and foliar application of myo-inositol (MYO) on growth, chlorophyll content, photosynthesis, antioxidant system, osmolyte accumulation, and gene expression in quinoa (Chenopodium quinoa L. var. Giza1). Our results show that salinity stress significantly decreased growth parameters such as plant height, fresh and dry weights of shoot and root, leaf area, number of leaves, chlorophyll content, net photosynthesis, stomatal conductance, transpiration, and Fv/Fm, with a more pronounced effect at higher NaCl concentrations. However, the exogenous application of MYO increased the growth and photosynthesis traits and alleviated the stress to a considerable extent. Salinity also significantly reduced the water potential and water use efficiency in plants under saline regime; however, exogenous application of myo-inositol coped with this issue. MYO significantly reduced the accumulation of hydrogen peroxide, superoxide, reduced lipid peroxidation, and electrolyte leakage concomitant with an increase in the membrane stability index. Exogenous application of MYO up-regulated the antioxidant enzymes' activities and the contents of ascorbate and glutathione, contributing to membrane stability and reduced oxidative damage. The damaging effects of salinity stress on quinoa were further mitigated by increased accumulation of osmolytes such as proline, glycine betaine, free amino acids, and soluble sugars in MYO-treated seedlings. The expression pattern of OSM34, NHX1, SOS1A, SOS1B, BADH, TIP2, NSY, and SDR genes increased significantly due to the application of MYO under both stressed and non-stressed conditions. Our results support the conclusion that exogenous MYO alleviates salt stress by involving antioxidants, enhancing plant growth attributes and membrane stability, and reducing oxidative damage to plants.

scholarly journals Exogenous Putrescine Enhances Salt Tolerance and Ginsenosides Content in Korean Ginseng (Panax ginseng Meyer) Sprouts

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1313
Author(s):  
Md. Jahirul Islam ◽  
Byeong Ryeol Ryu ◽  
Md. Obyedul Kalam Azad ◽  
Md. Hafizur Rahman ◽  
Md. Soyel Rana ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Salinity Stress ◽  
Soluble Sugar ◽  
Principal Component ◽  
Total Soluble Protein ◽  
Soluble Carbohydrate ◽  
Guaiacol Peroxidase ◽  
Salinity Treatment ◽  
Total Soluble Sugar ◽  
Biochemical Processes

The effect of exogenously applied putrescine (Put) on salt stress tolerance was investigated in Panax ginseng. Thirty-day-old ginseng sprouts were grown in salinized nutrient solution (150 mM NaCl) for five days, while the control sprouts were grown in nutrients solution. Putrescine (0.3, 0.6, and 0.9 mM) was sprayed on the plants once at the onset of salinity treatment, whereas control plants were sprayed with water only. Ginseng seedlings tested under salinity exhibited reduced plant growth and biomass production, which was directly interlinked with reduced chlorophyll and chlorophyll fluorescence due to higher reactive oxygen species (hydrogen peroxide; H2O2) and lipid peroxidation (malondialdehyde; MDA) production. Application of Put enhanced accumulation of proline, total soluble carbohydrate, total soluble sugar and total soluble protein. At the same time, activities of antioxidant enzymes like superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase in leaves, stems, and roots of ginseng seedlings were increased. Such modulation of physio-biochemical processes reduced the level of H2O2 and MDA, which indicates a successful adaptation of ginseng seedlings to salinity stress. Moreover, protopanaxadiol (PPD) ginsenosides enhanced by both salinity stress and exogenous Put treatment. On the other hand, protopanaxatriol (PPT) ginsenosides enhanced in roots and reduced in leaves and stems under salinity stress condition. In contrast, they enhanced by exogenous Put application in all parts of the plants for most cases, also evidenced by principal component analysis. Collectively, our findings provide an important prospect for the use of Put in modulating salinity tolerance and ginsenosides content in ginseng sprouts.

Protection of Italian ryegrass (Lolium multiflorum L.) seedlings from salinity stress following seed priming with L-methionine and casein hydrolysate

2020 ◽  
pp. 1-9
Author(s):  
Keum-Ah Lee ◽  
Youngnam Kim ◽  
Hossein Alizadeh ◽  
David W.M. Leung
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Salinity Stress ◽  
Lolium Multiflorum ◽  
Casein Hydrolysate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Italian Ryegrass ◽  
Significant Difference ◽  
Protein Amino Acids

Abstract Seed priming with water (hydropriming or HP) has been shown to be beneficial for seed germination and plant growth. However, there is little information on the effects of seed priming with amino acids and casein hydrolysate (CH) compared with HP, particularly in relation to early post-germinative seedling growth under salinity stress. In this study, Italian ryegrass seeds (Lolium multiflorum L.) were primed with 1 mM of each of the 20 protein amino acids and CH (200 mg l−1) before they were germinated in 0, 60 and 90 mM NaCl in Petri dishes for 4 d in darkness. Germination percentage (GP), radicle length (RL) and peroxidase (POD) activity in the root of 4-d-old Italian ryegrass seedlings were investigated. Generally, when the seeds were germinated in 0, 60 and 90 mM NaCl, there was no significant difference in GP of seeds among various priming treatments, except that a higher GP was observed in seeds of HP treatment compared with the non-primed seeds when incubated in 60 mM NaCl. When incubated in 60 and 90 mM NaCl, seedlings from seeds primed with L-methionine or CH exhibited greater RL (greater protection against salinity stress) and higher root POD activity than those from non-primed and hydro-primed seeds. Under salinity stress, there were higher levels of malondialdehyde (MDA) in the root of 4-d-old Italian ryegrass seedlings, a marker of oxidative stress, but seed priming with CH was effective in reducing the salinity-triggered increase in MDA content. These results suggest that priming with L-methionine or CH would be better than HP for the protection of seedling root growth under salinity stress and might be associated with enhanced antioxidative defence against salinity-induced oxidative stress.

Clade III cytokinin response factors share common roles in response to oxidative stress responses linked to cytokinin synthesis

2021 ◽  
Vol 72 (8) ◽  
pp. 3294-3306
Author(s):  
Ariel M Hughes ◽  
H Tucker Hallmark ◽  
Lenka Plačková ◽  
Ondrej Novák ◽  
Aaron M Rashotte
Keyword(s):  
Oxidative Stress ◽  
Transcription Factors ◽  
Stress Response ◽  
Stress Responses ◽  
Oxidative Stress Response ◽  
Response Factors ◽  
Ontology Term ◽  
Regulated Expression ◽  
Cytokinin Response ◽  
Oxidative Stress Responses

Abstract Cytokinin response factors (CRFs) are transcription factors that are involved in cytokinin (CK) response, as well as being linked to abiotic stress tolerance. In particular, oxidative stress responses are activated by Clade III CRF members, such as AtCRF6. Here we explored the relationships between Clade III CRFs and oxidative stress. Transcriptomic responses to oxidative stress were determined in two Clade III transcription factors, Arabidopsis AtCRF5 and tomato SlCRF5. AtCRF5 was required for regulated expression of >240 genes that are involved in oxidative stress response. Similarly, SlCRF5 was involved in the regulated expression of nearly 420 oxidative stress response genes. Similarities in gene regulation by these Clade III members in response to oxidative stress were observed between Arabidopsis and tomato, as indicated by Gene Ontology term enrichment. CK levels were also changed in response to oxidative stress in both species. These changes were regulated by Clade III CRFs. Taken together, these findings suggest that Clade III CRFs play a role in oxidative stress response as well as having roles in CK signaling.

scholarly journals Effects of 1-aminocyclopropane-1-carboxylate-deaminase–Producing Bacteria on Perennial Ryegrass Growth and Physiological Responses to Salinity Stress

2016 ◽  
Vol 141 (3) ◽  
pp. 233-241 ◽  
Author(s):  
Liang Cheng ◽  
Ning Zhang ◽  
Bingru Huang
Keyword(s):  
Salinity Stress ◽  
Perennial Ryegrass ◽  
Photochemical Efficiency ◽  
Acc Deaminase ◽  
Nutrition Status ◽  
Shoot Biomass ◽  
Leaf Water Content ◽  
Salinity Treatment ◽  
Plant Tolerance ◽  
Water Control

The accumulation of 1-aminocyclopropane-1-carboxylate (ACC), which is a precursor for ethylene production, in plant roots exposed to salinity stress can be detrimental to plant growth. The objectives of this study were to determine whether inoculating roots with bacteria containing deaminase enzymes that break down ACC (ACC-deaminase) could improve plant tolerance to salinity in perennial ryegrass (Lolium perenne) and to examine growth and physiological factors, as well as nutrition status of plants affected by the ACC-deaminase bacteria inoculation under salinity stress. Plants of perennial ryegrass (cv. Pangea) were inoculated with either Burkholderia phytofirmans PsJN or Burkholderia gladioli RU1 and irrigated with either fresh water (control) or a 250 mm NaCl solution to induce salinity stress. The bacterium-inoculated plants had less ACC content in shoots and roots under both nonstressed and salinity conditions. Salinity stress inhibited root and shoot growth, but the bacterium-inoculated plants exhibited higher visual turf quality (TQ), tiller number, root biomass, shoot biomass, leaf water content, and photochemical efficiency, as well as lower cellular electrolyte leakage (EL) under salinity stress. Plants inoculated with bacteria had lower sodium content and higher potassium to sodium ratios in shoots under salinity stress. Shoot and root nitrogen content and shoot potassium content increased, whereas shoot and root calcium, magnesium, iron, and aluminum content all decreased due to bacterial inoculation under salinity treatment. ACC-deaminase bacteria inoculation of roots was effective in improving salinity tolerance of perennial ryegrass and could be incorporated into turfgrass maintenance programs in salt-affected soils.

scholarly journals Accumulation of Phenylpropanoids in Tartary Buckwheat (Fagopyrum tataricum) under Salt Stress

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 739 ◽  
Author(s):  
Nam Su Kim ◽  
Soon-Jae Kwon ◽  
Do Manh Cuong ◽  
Jin Jeon ◽  
Jong Seok Park ◽  
...  
Keyword(s):  
Salinity Stress ◽  
Ion Homeostasis ◽  
Tartary Buckwheat ◽  
Anthocyanin Content ◽  
Salinity Treatment ◽  
Fagopyrum Tataricum ◽  
Time Treatment ◽  
Nacl Concentration ◽  
Ion Imbalance

Salinity stress affects plants by reducing the water potential and causing ion imbalance or disturbances in ion homeostasis and toxicity. Salinity stress frequently causes both osmotic and ionic stress in plants, resulting in the increase or decrease of certain secondary metabolites in plants. In this study, the effect of NaCl treatment on the nutritional quality of tartary buckwheat plants was studied by conducting an HPLC analysis of phenylpropanoid and anthocyanin content. It was observed that there was no significant change of color in tartary buckwheat during salt treatment. The accumulation of most phenylpropanoid compounds increased slightly in response to the NaCl concentration. The total phenylpropanoid content in tartary buckwheat was the highest at 100 mM NaCl treatment. Seven-day-old wheat plantlets treated with 100 mM NaCl for 2, 4, 6, and 8 days showed the highest accumulation of total phenylpropanoids at day 8 after treatment, while the content of most phenylpropanoids was higher than that in the control during this period. Although the development of tartary buckwheat slightly decreased with NaCl treatment and the accumulation of anthocyanin compounds did not change in plants with a diffident NaCl concentration and time treatment, the results suggest that the salinity treatment of tartary buckwheat causes antioxidant activity improvement by inducing an accumulation of flavonoid and phenolic compounds. However, since the anthocyanin content did not increase, the antioxidant effect of the treatment is not expected to be significant.

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