scholarly journals Exopolysaccharide Isolated from Lactobacillus plantarum L-14 Has Anti-Inflammatory Effects via the Toll-Like Receptor 4 Pathway in LPS-Induced RAW 264.7 Cells

2020 ◽  
Vol 21 (23) ◽  
pp. 9283
Author(s):  
Mijin Kwon ◽  
Jaehoon Lee ◽  
Sangkyu Park ◽  
Oh-Hee Kwon ◽  
Jeongmin Seo ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Lactobacillus Plantarum ◽  
The Body ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Proinflammatory Mediators ◽  
Anti Inflammatory ◽  
L 14

Inflammation is a biological response of the immune system to defend the body from negative stimulation. However, the excessive inflammatory response can damage host tissues and pose serious threats. Exopolysaccharide (EPS), one of the postbiotics, is secreted from lactic acid bacteria. Although many studies have described the beneficial effects of EPS, such as its anti-inflammatory and anti-oxidant effects, its underlying mechanisms have remained to be poorly understood. Thus, we identified that EPS obtained from Lactobacillus plantarum L-14 was a homogeneous polysaccharide primarily comprised of glucose. To examine these anti-inflammatory effects, an inflammatory response was induced by lipopolysaccharide (LPS) administration to mouse macrophage RAW 264.7 cells that were pretreated with EPS. The anti-inflammatory effects of EPS were identified by analyzing the changes within inflammatory markers at the molecular level. We demonstrate here that EPS suppressed proinflammatory mediators, such as cyclooxygenase-2, interleukin-6, tumor necrosis factor-α, and interleukin-1β, and downregulated the expression of an inducible nitric oxide synthase known to lead to oxidative stress. It was also confirmed that EPS had anti-inflammatory effects by blocking the interaction of LPS with Toll-like receptor 4 (TLR4), as demonstrated by using the known TLR4 inhibitor TAK-242. In addition, we found that EPS itself could suppress the expression of TLR4. Consequently, our data suggest that EPS can be a potential target for the development of natural product-derived medicine for treating inflammatory diseases related to TLR4.

Agrimonia pilosa Ledeb Root Extract: Anti-Inflammatory Activities of the Medicinal Herb in LPS-Induced Inflammation

2020 ◽  
Vol 48 (08) ◽  
pp. 1875-1893
Author(s):  
Da-Sol Kim ◽  
Kyoung-Eun Park ◽  
Yeon-Ju Kwak ◽  
Moon-Kyoung Bae ◽  
Soo-Kyung Bae ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Inflammatory Cytokines ◽  
Mapk Signaling ◽  
The Body ◽  
Raw 264.7 Cells ◽  
Root Extract ◽  
Raw 264.7 ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Pro Inflammatory Cytokines

Inflammation regulation is essential for maintaining healthy functions and normal homeostasis of the body. Porphyromonas gingivalis (P. gingivalis) is a gram-negative anaerobic bacterium and a major pathogen that causes oral inflammation and other systemic inflammations. This study aims to examine the anti-inflammatory effects of Agrimonia pilosa Ledeb root extracts (APL-ME) in Porphyromonas gingivalis LPS-induced RAW 264.7 cells and find anti-inflammatory effect compounds of APL-ME. The anti-inflammatory effects of APL-ME were evaluated anti-oxidant activity, cell viability, nitrite concentration, pro-inflammatory cytokines (interleukin-1[Formula: see text], interleukin-6, tumor necrosis factor (TNF)-[Formula: see text], and anti-inflammatory cytokine (interleukin-10 (IL-10)). Also, Inflammation related genes and proteins, cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS), expression were decreased by APL-ME and mitogen-activated protein kinase (MAPK) signaling proteins expression was regulated by APL-ME. Liquid chromatography-mass spectrometer (LC/MS)-MS analysis results indicated that several components were detected in APL-ME. Our study indicated that APL-ME suppressed nitrite concentrations, pro-inflammatory cytokines such as IL-1[Formula: see text], IL-6 and TNF-[Formula: see text] in P. gingivalis LPS induced RAW 264.7 cells. However, IL-10 expression was increased by ALP-ME. In addition, protein expressions of COX-2 and iNOS were inhibited APL-ME extracts dose-dependently. According to these results, APL-ME has anti-inflammatory effects in P. gingivalis LPS induced RAW 264.7 cells.

scholarly journals Anti-inflammatory effect of tricin isolated from Alopecurus aequalis Sobol. on the LPS-induced inflammatory response in RAW 264.7 cells

2016 ◽  
Vol 38 (5) ◽  
pp. 1614-1620 ◽  
Author(s):  
Byoung-Man Kang ◽  
Byoung-Kwan An ◽  
Won-Seok Jung ◽  
Ho-Kyung Jung ◽  
Jung-Hee Cho ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals A Novel Korean Red Ginseng Compound Gintonin Inhibited Inflammation by MAPK and NF-κB Pathways and Recovered the Levels of mir-34a and mir-93 in RAW 264.7 Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Evelyn Saba ◽  
Bo Ra Jeon ◽  
Da-Hye Jeong ◽  
Kija Lee ◽  
Youn-Kyoung Goo ◽  
...  
Keyword(s):  
Signal Transduction ◽  
The Body ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Therapeutic Effects ◽  
Raw 264.7 ◽  
Korean Red Ginseng ◽  
Health Promoting ◽  
Anti Inflammatory ◽  
First Time

The beneficial health promoting effects of ginseng from vitalizing the body to enhancing long life have been well explored very rapidly in the past few years. Up till now many ginsenosides have been discovered for their marvelous therapeutic effects. However during past three years, a novel ginseng compound has been discovered, called gintonin, that differs from other ginsenosides on the basis of its signal transduction and chemical nature. Gintonin has been widely studied for its anti-Alzheimer’s disease activities and other neuropathies. However, its anti-inflammatory activity remained unexplored. In our study we have reported for the first time the anti-inflammatory activity of gintonin on RAW 264.7 cells. We found that gintonin potently suppresses the nitric oxide production without any cytotoxicity at given doses and also efficiently suppressed the levels of proinflammatory cytokines. Moreover, it mediaes its signal transduction via MAPK and NF-κB pathways and revives the levels of mir-34a and mir-93. These findings are valuable for the anti-inflammatory effects of this new compound with particular reference to microRNA involvement in the ginseng family.

scholarly journals The anti-inflammatory effect of Taraxacum coreanum on lipopolysaccharide induced inflammatory response on RAW 264.7 cells

2014 ◽  
Vol 29 (6) ◽  
pp. 21-26 ◽  
Author(s):  
Min-Jun Kim ◽  
Gi-Sang Bae ◽  
Sun Bok Choi ◽  
Il-Joo Jo ◽  
Dong-Goo Kim ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Anti Inflammatory ◽  
Taraxacum Coreanum ◽  
Inflammatory Effect

scholarly journals Ginsenoside Rh2 Downregulates LPS-Induced NF-κB Activation through Inhibition of TAK1 Phosphorylation in RAW 264.7 Murine Macrophage

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Li-Hua Lian ◽  
Quan Jin ◽  
Shun-Zong Song ◽  
Yan-Ling Wu ◽  
Ting Bai ◽  
...  
Keyword(s):  
Inflammatory Diseases ◽  
Hypoxia Inducible Factor ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Nuclear Factor ◽  
Ginsenoside Rh2 ◽  
Toll Like Receptor ◽  
Inhibitory Effects ◽  
Toll Like Receptor 4 ◽  
Time Period

The present study was carried out to evaluate the inhibitory effects of ginsenoside Rh2 on nuclear-factor- (NF-)κB in lipopolysaccharide- (LPS-) activated RAW 264.7 murine macrophages. RAW 264.7 cells were pretreated with indicated concentrations of ginsenoside Rh2 for 1 h prior to the incubation of LPS (1 μg/mL) for indicated time period. Ginsenoside Rh2 reduced CD14 and Toll-like receptor 4 (TLR4) expressions 24 h after LPS stimulation. Furthermore, ginsenoside Rh2 significantly inhibited TGF-beta-activated kinase 1 (TAK1) phosphorylation 30 min after LPS stimulation. Ginsenoside Rh2 was further shown to inhibit NF-κB p65 translocation into the nucleus by suppressing IκB-αdegradation. Also, LPS increased mRNA expression of TNF-αand IL-1αtime-dependently, while TQ reduced TNF-αwithin 3 h and IL-1αwithin 1 h. And we firstly found that pretreatment of ginsenoside Rh2 successively inhibited hypoxia-inducible factor- (HIF-) 1αexpression increased by LPS. In conclusion, ginsenoside Rh2 may inhibit LPS-induced NF-κB activation and reduce HIF-1αaccumulation, suggesting that ginsenoside Rh2 may be considered as a potential therapeutic candidate for chronic inflammatory diseases.

scholarly journals Regulation of Proinflammatory Mediators via NF-κB and p38 MAPK-Dependent Mechanisms in RAW 264.7 Macrophages by Polyphenol Components Isolated from KoreaLonicera japonica THUNB

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Kwang-Il Park ◽  
Sang-Rim Kang ◽  
Hyeon-Soo Park ◽  
Do Hoon Lee ◽  
Arulkumar Nagappan ◽  
...  
Keyword(s):  
P38 Mapk ◽  
Nuclear Translocation ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Necrosis Factor Alpha ◽  
Proinflammatory Mediators ◽  
Mapk Activity ◽  
Extracellular Signal ◽  
Mitogen Activated Protein ◽  
Anti Inflammatory

Lonicera japonica THUNB., which abundantly contains polyphenols, has been used as a traditional medicine for thousands of years in East Asian countries because of the anti-inflammation properties. This study aimed to investigate the anti-inflammatory mechanism of polyphenol components isolated from KoreaL. japonica T.by nuclear factor-kappaB (NF-κB) and mitogen-activated protein kinases (MAPKs) pathway. Polyphenols significantly decreased lipopolysaccharide- (LPS-) induced mRNA and protein expression of inducible nitric oxide synthase and cyclooxygenase-2, as well as mRNA expression of tumor necrosis factor-alpha, interleukin- (IL-) 1β, and IL-6. Moreover, polyphenols inhibited nuclear translocation of NF-κB p65, phosphorylation/degradation of the inhibitor ofκB, and phosphorylation of p38 MAPK, whereas the extracellular signal-regulated kinase and Janus N-terminal kinase were not affected. These results indicate that polyphenol components isolated from KoreaL. japonica T.should have anti-inflammatory effect on LPS-stimulated RAW 264.7 cells through the decrease of proinflammatory mediators expression by suppressing NF-κB and p38 MAPK activity.

scholarly journals Immunobiotic Lactobacillus jensenii Modulates the Toll-Like Receptor 4-Induced Inflammatory Response via Negative Regulation in Porcine Antigen-Presenting Cells

2012 ◽  
Vol 19 (7) ◽  
pp. 1038-1053 ◽  
Author(s):  
Julio Villena ◽  
Rie Suzuki ◽  
Hitomi Fujie ◽  
Eriko Chiba ◽  
Takuya Takahashi ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Transforming Growth Factor ◽  
Functional Characterization ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Content Type ◽  
Lactobacillus Jensenii ◽  
Anti Inflammatory ◽  
Tlr4 Activation ◽  
Antigen Presenting

ABSTRACTPreviously, we demonstrated thatLactobacillus jenseniiTL2937 attenuates the inflammatory response triggered by activation of Toll-like receptor 4 (TLR-4) in porcine intestinal epithelial cells. In view of the critical importance of antigen-presenting cell (APC) polarization in immunoregulation, the objective of the present study was to examine the effect of strain TL2937 on the activation patterns of APCs from swine Peyer's patches (PPs). We demonstrated that direct exposure of porcine APCs toL. jenseniiin the absence of inflammatory signals increased expression of interleukin-10 (IL-10) and transforming growth factor β in CD172a+APCs and caused them to display tolerogenic properties. In addition, pretreatment of CD172a+APCs withL. jenseniiresulted in differential modulation of the production of pro- and anti-inflammatory cytokines in response to TLR4 activation. The immunomodulatory effect of strain TL2937 was not related to a downregulation of TLR4 but was related to an upregulation of the expression of three negative regulators of TLRs: single immunoglobulin IL-1-related receptor (SIGIRR), A20, and interleukin-1 receptor-associated kinase M (IRAK-M). Our results also indicated that TLR2 has an important role in the anti-inflammatory activity ofL. jenseniiTL2937, since anti-TLR2 antibodies blocked the upregulation of SIGIRR and IRAK-M in CD172a+APCs and the production of IL-10 in response to TLR4 activation. We performed, for the first time, a precise functional characterization of porcine APCs from PPs, and we demonstrated that CD172a+cells were tolerogenic. Our findings demonstrate that adherent cells and isolated CD172a+cells harvested from swine PPs were useful forin vitrostudy of the inflammatory responses in the porcine gut and the immunomodulatory effects of immunobiotic microorganisms.

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