scholarly journals A Novel HPLC-Based Method to Investigate on RNA after Fixation

2020 ◽  
Vol 21 (20) ◽  
pp. 7540
Author(s):  
Paolo Fattorini ◽  
Cristina Forzato ◽  
Domenico Tierno ◽  
Eleonora De Martino ◽  
Eros Azzalini ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Nucleic Acid ◽  
High Performance ◽  
Hplc Analysis ◽  
Enzymatic Digestion ◽  
Clinical Samples ◽  
Iso Standard ◽  
Set Up ◽  
Rna Preservation

RNA isolated from fixed and paraffin-embedded tissues is widely used in biomedical research and molecular pathology for diagnosis. In the present study, we have set-up a method based on high performance liquid chromatography (HPLC) to investigate the effects of different fixatives on RNA. By the application of the presented method, which is based on the Nuclease S1 enzymatic digestion of RNA extracts followed by a HPLC analysis, it is possible to quantify the unmodified nucleotide monophosphates (NMPs) in the mixture and recognize their hydroxymethyl derivatives as well as other un-canonical RNA moieties. The results obtained from a set of mouse livers fixed/embedded with different protocols as well from a set of clinical samples aged 0 to 30 years-old show that alcohol-based fixatives do not induce chemical modification of the nucleic acid under ISO standard recommendations and confirm that pre-analytical conditions play a major role in RNA preservation.

HPLC Analysis of Oxytetracycline Residues in Honey

1986 ◽  
Vol 49 (5) ◽  
pp. 383-388 ◽  
Author(s):  
PETER SPORNS ◽  
SUET KWAN ◽  
LAWRENCE A. ROTH
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aqueous Solutions ◽  
High Performance ◽  
Hplc Analysis ◽  
Extraction Procedure ◽  
Hplc Method ◽  
Limits Of Detection ◽  
Ph Values ◽  
Double Extraction

Oxytetracycline (OTC), also known commercially as Terramycin, was determined to be more stable in honey than in buffered aqueous solutions at similar pH values and temperatures. A rapid high performance liquid chromatography (HPLC) method was developed to detect and quantitate OTC using a 1:1 dilution (wt/wt) of honey samples in water. Using 355 nm as the wavelength of detection, amounts as low as 0.5 μg/ml could be detected in the above solution. The limits of detection were lowered considerably by a double extraction procedure.

ANTHOCYANIN, AGLYCONE AND AGLYCONE-SUGAR CONTENT OF FRUITS OF TEMPERATE NORTH AMERICAN SPECIES OF Gaylussacia (ERICACEAE)

1988 ◽  
Vol 68 (1) ◽  
pp. 247-253 ◽  
Author(s):  
J. R. BALLINGTON ◽  
W. E. BALLINGER ◽  
E. P. MANESS
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
North American ◽  
High Performance ◽  
Hplc Analysis ◽  
Sugar Content ◽  
North American Species ◽  
The Other ◽  
Phylogenetic Implications

HPLC analysis of the true huckleberry species Gaylussacia baccata, G. dumosa, G. frondosa, G. mosieri, and G. ursina identified the 3-monoarabinosides, 3-monogalactosides, and 3-monoglucosides of cyanidin, delphinidin, malvidin, peonidin, and petunidin. Gaylussacia brachycera contained all anthocyanins, except peonidin-3-arabinoside. Gaylussacia brachycera differed from other species in percent delphinidin-3-arabinoside. It was higher than the other species in percent of the aglycone delphinidin and lower in cyanidin, and also higher in percent of the sugar arabinose. There were no detectable differences among the other species for anthocyanins, aglycones, or aglycone-sugars. The phylogenetic implications of the similarities among species of Gaylussacia and Vaccinium in anthocyanins, aglycones, and aglycone-sugars of the fruit were discussed.Key words: High-performance liquid chromatography, huckleberries, blueberries, chemotaxonomy, taxonomy, biosystematics

scholarly journals PHARMACOKINETIC PROFILE AND INCURRED ESOMEPRAZOLE SAMPLE STABILITY IN PLASMA USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY - PHOTODIODE ARRAY

2019 ◽  
pp. 214-219
Author(s):  
YAHDIANA HARAHAP ◽  
AHMAD FARIS ◽  
SUNARSIH .
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Pharmacokinetic Profile ◽  
Clinical Samples ◽  
Plasma Samples ◽  
Elimination Phase ◽  
Sample Stability ◽  
Photodiode Array

Objective: Esomeprazole (ESO) is one of the proton-pump inhibitors and is used to treat gastroesophageal reflux. It is sensitive to low pH, heat,moisture, and oxidation, which often means that ESO in clinical samples is degraded at the time of storage, affecting analysis results. This study aimedto analyze the in vivo stability of ESO in subjects’ plasma samples by testing the incurred sample stability (ISS) of ESO in plasma following 7, 14, and28 days of storage at two concentrations close to Cmax and one concentration in the elimination phase.Methods: Samples were analyzed using high-performance liquid chromatography with a C18 column with detection at 300 nm using a photodiodearray detector. Lansoprazole was used as an internal standard.Results: The ESO pharmacokinetics profile in the plasma samples yielded the values of Cmax 704.57–1425.85 ng/mL; tmax is 2.25 h; and AUC0-t is2444 ng.h/mL. ISS testing of plasma samples values were 6.50%, 5.73%, and 4.57% on first Cmax concentration; 3.55%, 4.84%, and 3.68% on 2nd Cmaxconcentration; and 4.04%, 4.80%, and 4.98% on elimination phase concentration.Conclusion: ISS testing results of plasma samples from six healthy subjects who were administered doses of 40 mg of ESO stored for 28 days showedthat it fulfilled the acceptance criteria (<20%) of the 2011 EMEA Bioanalytical Guidelines with a %diff value in all incurred samples of 6.5%.

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