scholarly journals Proteomic and Transcriptomic Patterns during Lipid Remodeling in Nannochloropsis gaditana

2020 ◽  
Vol 21 (18) ◽  
pp. 6946
Author(s):  
Chris J. Hulatt ◽  
Irina Smolina ◽  
Adam Dowle ◽  
Martina Kopp ◽  
Ghana K. Vasanth ◽  
...  
Keyword(s):  
Lipid Body ◽  
Gene Transcript ◽  
Nitrogen Deprivation ◽  
Oil Accumulation ◽  
Nannochloropsis Gaditana ◽  
Marine Microalga ◽  
Global Correlation ◽  
Tandem Mass Tag ◽  
Phytoplankton Communities ◽  
Associated Proteins

Nutrient limited conditions are common in natural phytoplankton communities and are often used to increase the yield of lipids from industrial microalgae cultivations. Here we studied the effects of bioavailable nitrogen (N) and phosphorus (P) deprivation on the proteome and transcriptome of the oleaginous marine microalga Nannochloropsis gaditana. Turbidostat cultures were used to selectively apply either N or P deprivation, controlling for variables including the light intensity. Global (cell-wide) changes in the proteome were measured using Tandem Mass Tag (TMT) and LC-MS/MS, whilst gene transcript expression of the same samples was quantified by Illumina RNA-sequencing. We detected 3423 proteins, where 1543 and 113 proteins showed significant changes in abundance in N and P treatments, respectively. The analysis includes the global correlation between proteomic and transcriptomic data, the regulation of subcellular proteomes in different compartments, gene/protein functional groups, and metabolic pathways. The results show that triacylglycerol (TAG) accumulation under nitrogen deprivation was associated with substantial downregulation of protein synthesis and photosynthetic activity. Oil accumulation was also accompanied by a diverse set of responses including the upregulation of diacylglycerol acyltransferase (DGAT), lipase, and lipid body associated proteins. Deprivation of phosphorus had comparatively fewer, weaker effects, some of which were linked to the remodeling of respiratory metabolism.

Structure and function of seed lipid body-associated proteins

2008 ◽  
Vol 331 (10) ◽  
pp. 746-754 ◽  
Author(s):  
Zita Purkrtova ◽  
Pascale Jolivet ◽  
Martine Miquel ◽  
Thierry Chardot
Keyword(s):  
Lipid Body ◽  
Structure And Function ◽  
Seed Lipid ◽  
Associated Proteins ◽  
And Function

scholarly journals Transcriptomic survey reveals multiple adaptation mechanisms in response to nitrogen deprivation in marine Porphyridium cruentum

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259833
Author(s):  
Li Wei ◽  
Wuxin You ◽  
Zhengru Xu ◽  
Wenfei Zhang
Keyword(s):  
Lipid Metabolism ◽  
De Novo ◽  
Nitrate Assimilation ◽  
Porphyridium Cruentum ◽  
Metabolic Reprogramming ◽  
Nitrogen Stress ◽  
Nitrogen Deprivation ◽  
Marine Microalga ◽  
Nutrient Environment ◽  
Red Microalga

Single-cell red microalga Porphyridium cruentum is potentially considered to be the bioresource for biofuel and pharmaceutical production. Nitrogen is a kind of nutrient component for photosynthetic P. cruentum. Meanwhile, nitrogen stress could induce to accumulate some substances such as lipid and phycoerythrin and affect its growth and physiology. However, how marine microalga Porphyridium cruentum respond and adapt to nitrogen starvation remains elusive. Here, acclimation of the metabolic reprogramming to changes in the nutrient environment was studied by high-throughput mRNA sequencing in the unicellular red alga P. cruentum. Firstly, to reveal transcriptional regulation, de novo transcriptome was assembled and 8,244 unigenes were annotated based on different database. Secondly, under nitrogen deprivation, 2100 unigenes displayed differential expression (1134 upregulation and 966 downregulation, respectively) and some pathways including carbon/nitrogen metabolism, photosynthesis, and lipid metabolism would be reprogrammed in P. cruentum. The result demonstrated that nitrate assimilation (with related unigenes of 8–493 fold upregulation) would be strengthen and photosynthesis (with related unigenes of 6–35 fold downregulation) be impaired under nitrogen deprivation. Importantly, compared to other green algae, red microalga P. cruentum presented a different expression pattern of lipid metabolism in response to nitrogen stress. These observations will also provide novel insight for understanding adaption mechanisms and potential targets for metabolic engineering and synthetic biology in P. cruentum.

Light-dependent bicarbonate uptake and CO 2 efflux in the marine microalga Nannochloropsis gaditana

Planta ◽  
2000 ◽  
Vol 211 (1) ◽  
pp. 43-49 ◽  
Author(s):  
I. Emma Huertas ◽  
George S. Espie ◽  
Brian Colman ◽  
Luis M. Lubian
Keyword(s):  
Nannochloropsis Gaditana ◽  
Marine Microalga ◽  
Bicarbonate Uptake

scholarly journals Growth aspects of the marine microalga Nannochloropsis gaditana

2003 ◽  
Vol 20 (4-6) ◽  
pp. 237-242 ◽  
Author(s):  
Jorge M.S. Rocha ◽  
Juan E.C. Garcia ◽  
Marta H.F. Henriques
Keyword(s):  
Nannochloropsis Gaditana ◽  
Marine Microalga

scholarly journals Lipid Body Dynamics in Shoot Meristems: Production, Enlargement, and Putative Organellar Interactions and Plasmodesmal Targeting

2021 ◽  
Vol 12 ◽  
Author(s):  
Manikandan Veerabagu ◽  
Päivi L. H. Rinne ◽  
Morten Skaugen ◽  
Laju K. Paul ◽  
Christiaan van der Schoot
Keyword(s):  
Gene Expression ◽  
Lipid Droplet ◽  
Major Gene ◽  
Lipid Body ◽  
Rab Gtpase ◽  
Embryonic Cells ◽  
Interacting Protein ◽  
Associated Proteins ◽  
Myosin Xi

Post-embryonic cells contain minute lipid bodies (LBs) that are transient, mobile, engage in organellar interactions, and target plasmodesmata (PD). While LBs can deliver γ-clade 1,3-β-glucanases to PD, the nature of other cargo is elusive. To gain insight into the poorly understood role of LBs in meristems, we investigated their dynamics by microscopy, gene expression analyzes, and proteomics. In developing buds, meristems accumulated LBs, upregulated several LB-specific OLEOSIN genes and produced OLEOSINs. During bud maturation, the major gene OLE6 was strongly downregulated, OLEOSINs disappeared from bud extracts, whereas lipid biosynthesis genes were upregulated, and LBs were enlarged. Proteomic analyses of the LB fraction of dormant buds confirmed that OLEOSINs were no longer present. Instead, we identified the LB-associated proteins CALEOSIN (CLO1), Oil Body Lipase 1 (OBL1), Lipid Droplet Interacting Protein (LDIP), Lipid Droplet Associated Protein1a/b (LDAP1a/b) and LDAP3a/b, and crucial components of the OLEOSIN-deubiquitinating and degradation machinery, such as PUX10 and CDC48A. All mRFP-tagged LDAPs localized to LBs when transiently expressed in Nicotiana benthamiana. Together with gene expression analyzes, this suggests that during bud maturation, OLEOSINs were replaced by LDIP/LDAPs at enlarging LBs. The LB fraction contained the meristem-related actin7 (ACT7), “myosin XI tail-binding” RAB GTPase C2A, an LB/PD-associated γ-clade 1,3-β-glucanase, and various organelle- and/or PD-localized proteins. The results are congruent with a model in which LBs, motorized by myosin XI-k/1/2, traffic on F-actin, transiently interact with other organelles, and deliver a diverse cargo to PD.

scholarly journals Novel endogenous promoters for genetic engineering of the marine microalga Nannochloropsis gaditana CCMP526

2019 ◽  
Vol 44 ◽  
pp. 101708 ◽  
Author(s):  
Margaret Ramarajan ◽  
Michele Fabris ◽  
Raffaela M. Abbriano ◽  
Mathieu Pernice ◽  
Peter J. Ralph
Keyword(s):  
Genetic Engineering ◽  
Nannochloropsis Gaditana ◽  
Marine Microalga

A methodological study of adhesion dynamics in a batch culture of the marine microalga Nannochloropsis gaditana

2017 ◽  
Vol 23 ◽  
pp. 240-254 ◽  
Author(s):  
O. Zeriouh ◽  
J.V. Reinoso-Moreno ◽  
L. López-Rosales ◽  
B. Sierra-Martín ◽  
M.C. Cerón-García ◽  
...  
Keyword(s):  
Batch Culture ◽  
Methodological Study ◽  
Nannochloropsis Gaditana ◽  
Marine Microalga

Optimization of outdoor cultivation of the marine microalga Nannochloropsis gaditana in flat-panel reactors using industrial exhaust flue gases

2019 ◽  
Vol 32 (2) ◽  
pp. 809-819 ◽  
Author(s):  
Alberto Rodríguez-López ◽  
Francisco Javier Fernández-Acero ◽  
Roberto Andrés-Vallejo ◽  
Palmira Guarnizo-García ◽  
María Dolores Macías-Sánchez ◽  
...  
Keyword(s):  
Flue Gases ◽  
Outdoor Cultivation ◽  
Flat Panel ◽  
Nannochloropsis Gaditana ◽  
Marine Microalga ◽  
Industrial Exhaust
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