scholarly journals Overexpression of MdARD4 Accelerates Fruit Ripening and Increases Cold Hardiness in Tomato

2020 ◽  
Vol 21 (17) ◽  
pp. 6182
Author(s):  
Tianli Guo ◽  
Xiuzhi Zhang ◽  
Yuxing Li ◽  
Chenlu Liu ◽  
Na Wang ◽  
...  
Keyword(s):  
Cold Stress ◽  
Fruit Ripening ◽  
Cold Hardiness ◽  
Profile Analysis ◽  
Transcript Level ◽  
Ethylene Biosynthesis ◽  
Apple Fruit ◽  
Cellular Level ◽  
Expression Profile Analysis ◽  
Acireductone Dioxygenase

Ethylene plays an important role in stress adaptation and fruit ripening. Acireductone dioxygenase (ARD) is pivotal for ethylene biosynthesis. However, the response of ARD to fruit ripening or cold stress is still unclear. In this study, we identified three members of Malus ARD family, and expression profile analysis revealed that the transcript level of MdARD4 was induced during apple fruit ripening and after apple plants were being treated with cold stress. To investigate its function in cold tolerance and fruit ripening, MdARD4 was ectopically expressed in Solanum lycopersicum cultivar ‘Micro-Tom’, which has been considered as an excellent model plant for the study of fruit ripening. At the cellular level, the MdARD protein expressed throughout Nicotiana benthamiana epidermal cells. Overexpression of MdARD4 in tomato demonstrated that MdARD4 regulates the ethylene and carotenoid signaling pathway, increases ethylene and carotenoid concentrations, and accelerates fruit ripening. Furthermore, MdARD4 increased the antioxidative ability and cold hardiness in tomato. To conclude, MdARD4 may potentially be used in apple breeding to accelerate fruit ripening and increase cold hardiness.

scholarly journals Brassinosteroids Suppress Ethylene Biosynthesis via Transcription Factor BZR1 in Pear and Apple Fruit

2020 ◽  
Author(s):  
Yinglin Ji ◽  
Yi Qu ◽  
Zhongyu Jiang ◽  
Xin Su ◽  
Pengtao Yue ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fruit Ripening ◽  
Ethylene Production ◽  
Plant Hormone ◽  
Expression Profiles ◽  
Acc Synthase ◽  
Ethylene Biosynthesis ◽  
Apple Fruit ◽  
Pear Fruit ◽  
Climacteric Fruit

ABSTRACTThe plant hormone ethylene is important for the ripening of climacteric fruit, such as pear (Pyrus ussuriensis), and the brassinosteroid (BR) class of phytohormones affects ethylene biosynthesis during ripening, although via an unknown molecular mechanism. Here, we observed that exogenous BR treatment suppressed ethylene production during pear fruit ripening, and that the expression of the transcription factor PuBZR1 was enhanced by epibrassinolide (EBR) treatment during pear fruit ripening. PuBZR1 was shown to interact with PuACO1, which converts 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene, and suppress its activity. We also observed that BR-activated PuBZR1 bound to the promoters of PuACO1 and of PuACS1a, which encodes ACC synthase, and directly suppressed their transcription. Moreover, PuBZR1 suppressed the expression of transcription factor PuERF2 by binding its promoter, and PuERF2 bound to the promoters of PuACO1 and PuACS1a. We concluded that PuBZR1 indirectly suppresses the transcription of PuACO1 and PuACS1a through its regulation of PuERF2. Ethylene production and the expression profiles of the corresponding apple (Malus domestica) homologs showed similar changes following EBR treatment. Together, these results suggest that BR-activated BZR1 suppresses ACO1 activity and the expression of ACO1 and ACS1a, thereby reducing ethylene production during pear and apple fruit ripening. This likely represents a conserved mechanism by which exogenous BR suppresses ethylene biosynthesis during climacteric fruit ripening.One-sentence summaryBR-activated BZR1 suppresses ACO1 activity and expression of ACO1 and ACS1a, which encode two ethylene biosynthesis enzymes, thereby reducing ethylene production during pear and apple fruit ripening.

scholarly journals Identification and Analysis of Genes Involved in the Jasmonate Pathway in Response to Ethephon and 1-Methylcyclopropene during the Ripening of Apple Fruit

2017 ◽  
Vol 142 (3) ◽  
pp. 184-191 ◽  
Author(s):  
Jingyi Lv ◽  
Yonghong Ge ◽  
Canying Li ◽  
Mengyuan Zhang ◽  
Jianrong Li
Keyword(s):  
Signaling Pathway ◽  
Fruit Ripening ◽  
Expression Profiles ◽  
Ethylene Biosynthesis ◽  
Apple Fruit ◽  
Pcr Analysis ◽  
Allene Oxide ◽  
Ripening Stage ◽  
Early Ripening ◽  
Ethephon Treatment

Fruit ripening is a complex process involving many physiological changes and the dynamic interplay between different phytohormones. In addition to ethylene, jasmonates (JAs) have also been demonstrated to play an important role in the regulation of fruit ripening. However, the mechanisms underlying the interaction between these two pathways during fruit ripening are unknown. In recent years, research has been conducted to illustrate the effects of JAs on the ethylene biosynthesis and signaling pathway, but little is known regarding the effects of ethylene on JA biosynthesis and the signaling pathway during fruit ripening. Herein, we aimed to evaluate the effects of ethylene on JA biosynthesis in ripening apple (Malus ×domestica) fruit and on the expression of key genes involved in the JA biosynthesis and the signaling pathway. For this purpose, we treated apple fruit with ethephon and 1-methylcyclopropene (1-MCP) at commercial maturity. Our data indicated that endogenous JA content and allene oxide synthase (AOS) activity were reduced by ethephon treatment at the early ripening stage, whereas they were enhanced by 1-MCP treatment at the late ripening stage. Quantitative real-time polymerase chain reaction (PCR) analysis revealed that the expression profiles of three AOS genes (MdAOS2, MdAOS3, and MdAOS5) and two lipoxygenase (LOX) genes (MdLOX22 and MdLOX28) showed similar trends with the change of AOS activity in all groups during fruit ripening. The expression of MdLOX21 and MdLOX23 was in accordance with the change of ethylene production on ripening, and it was positively regulated by ethylene, whereas the opposite effect was observed for MdLOX39 expression. The transcription of MdLOX310 and MdLOX61 appeared unaffected by ethylene during fruit ripening. Three jasmonate ZIM-domain (JAZ) genes (MdJAZ9, MdJAZ10, and MdJAZ18) were differentially upregulated by ethephon treatment whereas being downregulated by 1-MCP treatment during fruit ripening. Expression of MdJAZ13 and MdJAZ14 was downregulated at the early ripening stage by both treatments. Our results suggested regulating roles of ethylene on the JA biosynthesis and signaling pathway during fruit ripening and senescence.

scholarly journals Gene expression profile analysis of pig muscle in response to cold stress

2016 ◽  
Vol 45 (1) ◽  
pp. 195-198 ◽  
Author(s):  
Zhang Dong-jie ◽  
Liu Di ◽  
Wang Liang ◽  
Wang Wen-tao ◽  
He Xin-miao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cold Stress ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Profile Analysis ◽  
Expression Profile Analysis ◽  
Pig Muscle

Cloning and expression profile analysis C1q-binding protein (PmC1qBP) in Penaeus monodon

2013 ◽  
Vol 18 (4) ◽  
pp. 774-781 ◽  
Author(s):  
Xianjun LIU ◽  
Lishi YANG ◽  
Jianhua HUANG ◽  
Falin ZHOU ◽  
Qibin YANG ◽  
...  
Keyword(s):  
Expression Profile ◽  
Profile Analysis ◽  
Binding Protein ◽  
Penaeus Monodon ◽  
Cloning And Expression ◽  
Expression Profile Analysis

scholarly journals Expression profile analysis reveals hub genes that are associated with immune system dysregulation in primary myelofibrosis

Hematology ◽  
2021 ◽  
Vol 26 (1) ◽  
pp. 478-490
Author(s):  
Haotian Ma ◽  
Jincen Liu ◽  
Zilong Li ◽  
Huaye Xiong ◽  
Yulei Zhang ◽  
...  
Keyword(s):  
Immune System ◽  
Expression Profile ◽  
Profile Analysis ◽  
Primary Myelofibrosis ◽  
Hub Genes ◽  
Expression Profile Analysis
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