scholarly journals Phenylpropanoids Are Connected to Cell Wall Fortification and Stress Tolerance in Avocado Somatic Embryogenesis

2020 ◽  
Vol 21 (16) ◽  
pp. 5679
Author(s):  
Carol A. Olivares-García ◽  
Martín Mata-Rosas ◽  
Carolina Peña-Montes ◽  
Francisco Quiroz-Figueroa ◽  
Aldo Segura-Cabrera ◽  
...  
Keyword(s):  
Cell Wall ◽  
Somatic Embryogenesis ◽  
Stress Responses ◽  
Building Blocks ◽  
Phenylpropanoid Pathway ◽  
Phenylpropanoid Metabolism ◽  
Coumaric Acid ◽  
Response Data ◽  
Low Efficiency ◽  
Cell Wall Reinforcement

Somatic embryogenesis (SE) is a valuable model for understanding the mechanism of plant embryogenesis and a tool for the mass production of plants. However, establishing SE in avocado has been complicated due to the very low efficiency of embryo induction and plant regeneration. To understand the molecular foundation of the SE induction and development in avocado, we compared embryogenic (EC) and non-embryogenic (NEC) cultures of two avocado varieties using proteomic and metabolomic approaches. Although Criollo and Hass EC exhibited similarities in the proteome and metabolome profile, in general, we observed a more active phenylpropanoid pathway in EC than NEC. This pathway is associated with the tolerance of stress responses, probably through the reinforcement of the cell wall and flavonoid production. We could corroborate that particular polyphenolics compounds, including p-coumaric acid and t-ferulic acid, stimulated the production of somatic embryos in avocado. Exogen phenolic compounds were associated with the modification of the content of endogenous polyphenolic and the induction of the production of the putative auxin-a, adenosine, cellulose and 1,26-hexacosanediol-diferulate. We suggest that in EC of avocado, there is an enhanced phenylpropanoid metabolism for the production of the building blocks of lignin and flavonoid compounds having a role in cell wall reinforcement for tolerating stress response. Data are available at ProteomeXchange with the identifier PXD019705.

Lignin composition and timing of cell wall lignification are involved in Brassica napus resistance to Sclerotinia sclerotiorum stem rot

2021 ◽  
Author(s):  
Andreas von Tiedemann ◽  
Birger Koopmann ◽  
Kerstin Hoech
Keyword(s):  
Cell Wall ◽  
Brassica Napus ◽  
Sclerotinia Sclerotiorum ◽  
Plant Defence ◽  
Phenylpropanoid Pathway ◽  
Stem Rot ◽  
Phenylpropanoid Metabolism ◽  
Sclerotinia Stem Rot ◽  
Cell Wall Modification ◽  
Lignin Deposition

Sclerotinia stem rot (SSR) is an economically and globally significant disease in oilseed rape (Brassica napus L.) caused by the necrotrophic ascomycete Sclerotinia sclerotiorum. This study explored the role of cell wall reinforcement by lignin as a relevant factor for effective plant defence against attack of this pathogen. Expression of key genes in the phenylpropanoid pathway and the induced synthesis of lignin in infected stem tissues were investigated in a study comparing a susceptible (Loras) and a moderately resistant cultivar (Zhongyou 821, ZY821). Data revealed an earlier and more rapid defence activation in ZY821 through up-regulation of transcript levels of genes related to key steps in the phenylpropanoid pathway associated with enhanced lignin deposition in the resistant B. napus genotype. Expression level of BnCAD5, encoding a cinnamyl alcohol dehydrogenase, responsible for conversion of monolignol to lignin, was more rapidly up-regulated in ZY821 than Loras. The similar expression pattern of BnCAD5 and the gene BnF5H, encoding for the ferulate-5-hydroxylase which catalyses the synthesis of syringyl (S) lignin precursors, suggests that BnCAD5 is involved in the S lignin formation. Histological observations confirmed these results, showing an earlier increase of S lignin deposition in the infected resistant genotype. Deposition of guaiacyl (G) lignin was detected in both genotypes and is thus considered a component of basal, cultivar-independent defence response of B. napus to stem rot. The results indicate the importance of cell wall modification for quantitative stem rot resistance by responses in the phenylpropanoid metabolism generating distinct lignin types on different temporal scales.

Plant cell wall reinforcement in the disease-resistance response: molecular composition and regulation

1995 ◽  
Vol 73 (S1) ◽  
pp. 511-517 ◽  
Author(s):  
Ulrich Matern ◽  
Bernhard Grimmig ◽  
Richard Edward Kneusel
Keyword(s):  
Cell Wall ◽  
Disease Resistance ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
De Novo ◽  
Alternative Pathway ◽  
Petroselinum Crispum ◽  
Phenylpropanoid Metabolism ◽  
Resistance Response ◽  
Cell Wall Reinforcement

The disease-resistance response of plant cells is composed of a multitude of biochemical events, and the activation of one of these, the phenylpropanoid metabolism, is pivotal for the survival of cells under stress conditions. The basic features of this facet of the disease-resistance response are beginning to be unraveled in model plant cell culture systems. These studies revealed a novel, alternative pathway for the synthesis of cell wall bound hydroxycinnamoyl esters and lignin. The investigations have, therefore, set the stage for a detailed analysis of the induction process that includes fast, posttranslational activation mechanisms as well as de novo enzyme synthesis. The biosynthesis of phenolic compounds destined for the cell wall is considered to reach far beyond the mere physical strengthening of the cells and includes additional functions, e.g., the release of antimycotic hydroxybenzaldehydes, which are vital for stress compensation. Key words: elicitor-induced phenylpropanoids, cell wall reinforcement, hydroxycinnamoyl esters, lignin, caffeoyl-CoA-specific 3-O-methyltransferase, disease resistance response, parsley (Petroselinum crispum) cell cultures.

scholarly journals The Craterostigma plantagineum protein kinase CpWAK1 interacts with pectin and integrates different environmental signals in the cell wall

Planta ◽  
2021 ◽  
Vol 253 (5) ◽  
Author(s):  
Peilei Chen ◽  
Valentino Giarola ◽  
Dorothea Bartels
Keyword(s):  
Cell Wall ◽  
Stress Responses ◽  
Expression Profiles ◽  
Phylogenetic Analyses ◽  
Cell Wall Protein ◽  
Biological Processes ◽  
Environmental Signals ◽  
High Affinity ◽  
Craterostigma Plantagineum ◽  
Receptor Kinases

Abstract Main conclusion The cell wall protein CpWAK1 interacts with pectin, participates in decoding cell wall signals, and induces different downstream responses. Abstract Cell wall-associated protein kinases (WAKs) are transmembrane receptor kinases. In the desiccation-tolerant resurrection plant Craterostigma plantagineum, CpWAK1 has been shown to be involved in stress responses and cell expansion by forming a complex with the C. plantagineum glycine-rich protein1 (CpGRP1). This prompted us to extend the studies of WAK genes in C. plantagineum. The phylogenetic analyses of WAKs from C. plantagineum and from other species suggest that these genes have been duplicated after species divergence. Expression profiles indicate that CpWAKs are involved in various biological processes, including dehydration-induced responses and SA- and JA-related reactions to pathogens and wounding. CpWAK1 shows a high affinity for “egg-box” pectin structures. ELISA assays revealed that the binding of CpWAKs to pectins is modulated by CpGRP1 and it depends on the apoplastic pH. The formation of CpWAK multimers is the prerequisite for the CpWAK–pectin binding. Different pectin extracts lead to opposite trends of CpWAK–pectin binding in the presence of Ca2+ at pH 8. These observations demonstrate that CpWAKs can potentially discriminate and integrate cell wall signals generated by diverse stimuli, in concert with other elements, such as CpGRP1, pHapo, Ca2+[apo], and via the formation of CpWAK multimers.

scholarly journals The Aspergillus niger MADS-box transcription factor RlmA is required for cell wall reinforcement in response to cell wall stress

2005 ◽  
Vol 58 (1) ◽  
pp. 305-319 ◽  
Author(s):  
Robbert A. Damveld ◽  
Mark Arentshorst ◽  
Angelique Franken ◽  
Patricia A. VanKuyk ◽  
Frans M. Klis ◽  
...  
Keyword(s):  
Cell Wall ◽  
Transcription Factor ◽  
Aspergillus Niger ◽  
Wall Stress ◽  
Mads Box ◽  
Cell Wall Stress ◽  
Cell Wall Reinforcement

scholarly journals Hypolignification: A Decisive Factor in the Development of Hyperhydricity

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2625
Author(s):  
Nurashikin Kemat ◽  
Richard G. F. Visser ◽  
Frans A. Krens
Keyword(s):  
Cell Wall ◽  
Lignin Content ◽  
Coumaric Acid ◽  
Wild Type ◽  
Higher Plant ◽  
Gene Coding ◽  
Phenylpropanoid Biosynthesis ◽  
Total Lignin ◽  
Total Lignin Content

One of the characteristics of hyperhydric plants is the reduction of cell wall lignification (hypolignification), but how this is related to the observed abnormalities of hyperhydricity (HH), is still unclear. Lignin is hydrophobic, and we speculate that a reduction in lignin levels leads to more capillary action of the cell wall and consequently to more water in the apoplast. p-coumaric acid is the hydroxyl derivative of cinnamic acid and a precursor for lignin and flavonoids in higher plant. In the present study, we examined the role of lignin in the development of HH in Arabidopsis thaliana by checking the wild-types (Ler and Col-0) and mutants affected in phenylpropanoid biosynthesis, in the gene coding for cinnamate 4-hydroxylase, C4H (ref3-1 and ref3-3). Exogenously applied p-coumaric acid decreased the symptoms of HH in both wild-type and less-lignin mutants. Moreover, the results revealed that exogenously applied p-coumaric acid inhibited root growth and increased the total lignin content in both wild-type and less-lignin mutants. These effects appeared to diminish the symptoms of HH and suggest an important role for lignin in HH.

scholarly journals Altered metal distribution in the sr45-1 Arabidopsis mutant causes developmental defects

2021 ◽  
Author(s):  
Steven Fanara ◽  
Marie Schloesser ◽  
Marc Hanikenne ◽  
Patrick Motte
Keyword(s):  
Stress Responses ◽  
Molecular Mechanisms ◽  
Phenylpropanoid Pathway ◽  
Histochemical Staining ◽  
Metal Distribution ◽  
Iron Starvation ◽  
Developmental Defects ◽  
Developmental Abnormalities ◽  
Arabidopsis Mutant ◽  
Root Tissues

The plant SR (serine/arginine-rich) splicing factor SR45 plays important roles in several biological processes, such as splicing, DNA methylation, innate immunity, glucose regulation and ABA signaling. A homozygous Arabidopsis sr45-1 null mutant is viable, but exhibits diverse phenotypic alterations, including delayed root development, late flowering, shorter siliques with fewer seeds, narrower leaves and petals, and unusual numbers of floral organs. Here, we report that the sr45-1 mutant presents an unexpected constitutive iron deficiency phenotype characterized by altered metal distribution in the plant. RNA-Sequencing highlighted severe perturbations in metal homeostasis, phenylpropanoid pathway, oxidative stress responses, and reproductive development. Ionomic quantification and histochemical staining revealed strong iron accumulation in the sr45-1 root tissues accompanied by an iron starvation in aerial parts. We showed that some sr45-1 developmental abnormalities can be complemented by exogenous iron supply. Our findings provide new insight into the molecular mechanisms governing the phenotypes of the sr45-1 mutant.

scholarly journals The plant cell wall integrity maintenance and immune signaling systems cooperate to control stress responses inArabidopsis thaliana

2018 ◽  
Vol 11 (536) ◽  
pp. eaao3070 ◽  
Author(s):  
Timo Engelsdorf ◽  
Nora Gigli-Bisceglia ◽  
Manikandan Veerabagu ◽  
Joseph F. McKenna ◽  
Lauri Vaahtera ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Stress Responses ◽  
Plant Cell Wall ◽  
Cell Wall Integrity ◽  
Immune Signaling ◽  
Signaling Systems

Autophagy-inhibiting biomimetic nanodrugs enhance photothermal therapy and boost antitumor immunity

2022 ◽  
Author(s):  
Peiying Huang ◽  
Yin Yin Zhu ◽  
Hao Zhong ◽  
Peiling Chen ◽  
Qunying Shi ◽  
...  
Keyword(s):  
Low Temperature ◽  
Tumor Cells ◽  
Stress Responses ◽  
Photothermal Therapy ◽  
Tumor Recurrence ◽  
Antitumor Immunity ◽  
Blood Clearance ◽  
Low Efficiency

The instinctive protective stress responses of tumor cells hamper the low-temperature photothermal therapy (LTPTT), resulting in tumor recurrence and metastasis. The rapid blood clearance and low-efficiency tumor enrichment of nanomedicines...

Dimethylarsinic acid is the causal agent inducing rice straighthead disease

2020 ◽  
Vol 71 (18) ◽  
pp. 5631-5644 ◽  
Author(s):  
Zhong Tang ◽  
Yijie Wang ◽  
Axiang Gao ◽  
Yuchen Ji ◽  
Baoyun Yang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Stress Responses ◽  
Arsenic Species ◽  
Causal Agent ◽  
Dimethylarsinic Acid ◽  
Cell Wall Modification ◽  
Physiological Disorder ◽  
Field Surveys ◽  
Methyltransferase Gene ◽  
Oxidative Stress Responses

Abstract Straighthead disease is a physiological disorder in rice with symptoms of sterile spikelets, distorted husks, and erect panicles. Methylated arsenic species have been implicated as the causal agent of the disease, but direct evidence is lacking. Here, we investigated whether dimethylarsinic acid (DMA) causes straighthead disease and its effect on the transcriptome of young panicles. DMA addition caused typical straighthead symptoms in hydroponic culture, which were alleviated by silicon addition. DMA addition to soil at the tillering to flowering stages induced straighthead disease. Transgenic rice expressing a bacterial arsenite methyltransferase gene gained the ability to methylate arsenic to mainly DMA, with the consequence of inducing straighthead disease. Field surveys showed that seed setting rate decreased with increasing DMA concentration in the husk, with an EC50 of 0.18 mg kg−1. Transcriptomic analysis showed that 364 and 856 genes were significantly up- and down-regulated, respectively, in the young panicles of DMA-treated plants compared with control, whereas Si addition markedly reduced the number of genes affected. Among the differentially expressed genes, genes related to cell wall modification and oxidative stress responses were the most prominent, suggesting that cell wall metabolism is a sensitive target of DMA toxicity and silicon protects against this toxicity.

scholarly journals Thermophilic Degradation of Hemicellulose, a Critical Feedstock in the Production of Bioenergy and Other Value-Added Products

2020 ◽  
Vol 86 (7) ◽  
Author(s):  
Isaac Cann ◽  
Gabriel V. Pereira ◽  
Ahmed M. Abdel-Hamid ◽  
Heejin Kim ◽  
Daniel Wefers ◽  
...  
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Plant Biomass ◽  
Animal Feed ◽  
Value Added ◽  
Building Blocks ◽  
Economic Feasibility ◽  
Renewable Fuels ◽  
Microbial Enzymes

ABSTRACT Renewable fuels have gained importance as the world moves toward diversifying its energy portfolio. A critical step in the biomass-to-bioenergy initiative is deconstruction of plant cell wall polysaccharides to their unit sugars for subsequent fermentation to fuels. To acquire carbon and energy for their metabolic processes, diverse microorganisms have evolved genes encoding enzymes that depolymerize polysaccharides to their carbon/energy-rich building blocks. The microbial enzymes mostly target the energy present in cellulose, hemicellulose, and pectin, three major forms of energy storage in plants. In the effort to develop bioenergy as an alternative to fossil fuel, a common strategy is to harness microbial enzymes to hydrolyze cellulose to glucose for fermentation to fuels. However, the conversion of plant biomass to renewable fuels will require both cellulose and hemicellulose, the two largest components of the plant cell wall, as feedstock to improve economic feasibility. Here, we explore the enzymes and strategies evolved by two well-studied bacteria to depolymerize the hemicelluloses xylan/arabinoxylan and mannan. The sets of enzymes, in addition to their applications in biofuels and value-added chemical production, have utility in animal feed enzymes, a rapidly developing industry with potential to minimize adverse impacts of animal agriculture on the environment.

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