scholarly journals Metabolic Syndrome, Clusterin and Elafin in Patients with Psoriasis Vulgaris

2020 ◽  
Vol 21 (16) ◽  
pp. 5617
Author(s):  
Drahomira Holmannova ◽  
Pavel Borsky ◽  
Lenka Borska ◽  
Ctirad Andrys ◽  
Kvetoslava Hamakova ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Psoriasis Vulgaris ◽  
Inflammatory Diseases ◽  
Pathological Condition ◽  
Serum Levels ◽  
Healthy Controls ◽  
Immune System Dysfunction ◽  
Wide Range ◽  
Pasi Score ◽  
Biochemical Analyses

Background: Psoriasis is a pathological condition characterized by immune system dysfunction and inflammation. Patients with psoriasis are more likely to develop a wide range of disorders associated with inflammation. Serum levels of various substances and their combinations have been associated with the presence of the disease (psoriasis) and have shown the potential to reflect its activity. The aim of the present study is to contribute to the elucidation of pathophysiological links between psoriasis, its pro-inflammatory comorbidity metabolic syndrome (MetS), and the expression of clusterin and elafin, which are reflected in the pathophysiological “portfolio” of both diseases. Material and methods: Clinical examinations (PASI score), ELISA (clusterin, elafin), and biochemical analyses (parameters of MetS) were performed. Results: We found that patients with psoriasis were more often afflicted by MetS, compared to the healthy controls. Clusterin and elafin levels were higher in the patients than in the controls but did not correlate to the severity of psoriasis. Conclusion: Our data suggest that patients with psoriasis are more susceptible to developing other systemic inflammatory diseases, such as MetS. The levels of clusterin and elafin, which are tightly linked to inflammation, were significantly increased in the patients, compared to the controls, but the presence of MetS in patients did not further increase these levels.

Enrichment of IGF-1R and PPARγ signalling pathways in orbital inflammatory diseases: steps toward understanding pathogenesis

2021 ◽  
pp. bjophthalmol-2020-318330
Author(s):  
Rohan Verma ◽  
Dongseok Choi ◽  
Allison J Chen ◽  
Christina A Harrington ◽  
David J Wilson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inflammatory Diseases ◽  
Target Therapy ◽  
Signalling Pathways ◽  
Peroxisome Proliferator ◽  
Healthy Controls ◽  
Gene Expressions ◽  
Peroxisome Proliferator Activated Receptor ◽  
Orbital Inflammation ◽  
Wide Range

BackgroundOrbital inflammatory disease (OID) encompasses a wide range of pathology including thyroid-associated orbitopathy (TAO), granulomatosis with polyangiitis (GPA), sarcoidosis and non-specific orbital inflammation (NSOI), accounting for up to 6% of orbital diseases. Understanding the underlying pathophysiology of OID can improve diagnosis and help target therapy.AimsTo test the hypothesis that shared signalling pathways are activated in different forms of OID.MethodsIn this secondary analysis, pathway analysis was performed on the previously reported differentially expressed genes from orbital adipose tissue using patients with OID and healthy controls who were characterised by microarray. For the original publications, tissue specimens were collected from oculoplastic surgeons at 10 international centres representing four countries (USA, Canada, Australia and Saudi Arabia). Diagnoses were independently confirmed by two masked ocular pathologists (DJW, HEG). Gene expression profiling analysis was performed at the Oregon Health & Science University. Eighty-three participants were included: 25 with TAO, 6 with orbital GPA, 7 with orbital sarcoidosis, 25 with NSOI and 20 healthy controls.ResultsAmong the 83 subjects (mean (SD) age, 52.8 (18.3) years; 70% (n=58) female), those with OID demonstrated perturbation of the downstream gene expressions of the IGF-1R (MAPK/RAS/RAF/MEK/ERK and PI3K/Akt/mTOR pathways), peroxisome proliferator-activated receptor-γ (PPARγ), adipocytokine and AMPK signalling pathways compared with healthy controls. Specifically, GPA samples differed from controls in gene expression within the insulin-like growth factor-1 receptor (IGF-1R, PI3K-Akt (p=0.001), RAS (p=0.005)), PPARγ (p=0.002), adipocytokine (p=0.004) or AMPK (p=<0.001) pathways. TAO, sarcoidosis and NSOI samples were also found to have statistically significant differential gene expression in these pathways.ConclusionsAlthough OID includes a heterogenous group of pathologies, TAO, GPA, sarcoidosis and NSOI share enrichment of common gene signalling pathways, namely IGF-1R, PPARγ, adipocytokine and AMPK. Pathway analyses of gene expression suggest that other forms of orbital inflammation in addition to TAO may benefit from blockade of IGF-1R signalling pathways.

scholarly journals The Impact of Psoriasis and Metabolic Syndrome on the Systemic Inflammation and Oxidative Damage to Nucleic Acids

2020 ◽  
Vol 2020 ◽  
pp. 1-9 ◽  
Author(s):  
Drahomira Holmannova ◽  
Lenka Borska ◽  
Ctirad Andrys ◽  
Pavel Borsky ◽  
Jan Kremlacek ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Serum Level ◽  
Oxidative Damage ◽  
Nucleic Acids ◽  
Systemic Inflammation ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wide Range ◽  
Systemic Inflammatory Disease ◽  
The Impact

Background. Psoriasis is a chronic systemic inflammatory disease associated with a wide range of comorbidities, including metabolic syndrome (MetS). Serum calprotectin, ANGPTL8, and oxidative damage to nucleic acids might be associated with both diseases. The presented study describes the influence of psoriasis and MetS on the serum levels of markers of systemic inflammation (calprotectin and ANGPTL8) and markers of oxidative damage to nucleic acids. The applicability of serum levels of calprotectin and ANGPTL8 for monitoring of the activity of psoriasis (diagnostic markers) is also evaluated. Methods. Clinical examination (PASI score, MetS), enzyme-linked immunosorbent assay (ELISA), and Enzyme Immunoassay (EIA). Serum calprotectin, ANGPTL8, 8-hydroxy-2′-deoxyguanosine, 8-hydroxyguanosine, and 8-hydroxyguanine. Results and Conclusions. The psoriasis significantly increased the serum level of calprotectin and the serum level of oxidative damage to nucleic acids, however not the serum level of ANGPTL8. The presence of MetS did not significantly affect the serum levels of calprotectin, ANGPTL8, and oxidative damage to nucleic acids in either psoriasis patients or controls. It seems that the serum level of calprotectin (but not the serum level of ANGPTL8) could be used as a biomarker for monitoring the activity of psoriasis.

scholarly journals Geographic Tongue and Fissured Tongue in 348 Patients with Psoriasis: Correlation with Disease Severity

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Bruna L. S. Picciani ◽  
Thays T. Souza ◽  
Vanessa de Carla B. Santos ◽  
Tábata A. Domingos ◽  
Sueli Carneiro ◽  
...  
Keyword(s):  
Disease Severity ◽  
Psoriasis Vulgaris ◽  
Oral Lesions ◽  
Healthy Controls ◽  
Cutaneous Lesions ◽  
Pasi Score ◽  
Psoriasis Severity ◽  
Fissured Tongue

Geographic tongue (GT) and fissured tongue (FT) are the more frequent oral lesions in patients with psoriasis. The aims of this study were to compare the prevalence of GT/FT between psoriasis group (PG) and healthy controls (HC) and investigate the correlation between GT/FT and psoriasis severity using the PASI and age of psoriasis onset. Three hundred and forty-eight PG and 348 HC were selected. According to the age of psoriasis onset, the individuals were classified as having early psoriasis and late psoriasis. The severity of vulgaris psoriasis was determined according to PASI. A follow-up was conducted in patients with psoriasis vulgaris (PV) with GT to evaluate the progression of oral and cutaneous lesions. The FT and GT were more frequent in PG than in HC. The incidence of GT was higher in patients with early psoriasis and that of FT in late-psoriasis. There is association between psoriasis intensity and GT; and a higher monthly decrease of PASI score in patients without GT. The presence of GT and FT is higher in PG than in the HC. GT is associated with disease severity and may be a marker of the psoriasis severity.

scholarly journals Serum Levels of Selected Th17 and Th22 Cytokines in Psoriatic Patients

2013 ◽  
Vol 35 ◽  
pp. 625-631 ◽  
Author(s):  
Anna Michalak-Stoma ◽  
Joanna Bartosińska ◽  
Małgorzata Kowal ◽  
Maria Juszkiewicz-Borowiec ◽  
Agnieszka Gerkowicz ◽  
...  
Keyword(s):  
Inflammatory Disease ◽  
Serum Levels ◽  
Skin Lesions ◽  
Psoriatic Skin ◽  
Control Group ◽  
Healthy Controls ◽  
T Helper ◽  
Systemic Inflammatory Disease ◽  
Pasi Score ◽  
Positive Correlations

Introduction. Psoriasis is a T cell-mediated inflammatory disease in which pathogenesis T helper (Th) lymphocytes (Th1, Th17, and Th22) play an important role. The aim of the study was to assess the serum levels of some cytokines involved in the Th17 and Th22 responses in psoriatic patients.Material and Methods. The study comprised 60 psoriatic patients and 30 healthy controls. In the serum collected from psoriatic patients and healthy controls, the concentrations of IL-6, IL-12, IL-17, IL-20, IL-22, and IL-23 were examined with ELISA kits. Severity of psoriatic skin lesions was assessed by means of PASI, BSA, and PGA scores.Results. IL-6, IL-20, and IL-22 concentrations were significantly higher in psoriatic patients in comparison with the control group. The positive correlations between the concentrations of IL-22 and IL-20 and severity of psoriasis assessed with PASI and BSA scores as well as IL-17 and PASI score were found. There was also a positive correlation between IL-23 and IL-17 concentrations.Conclusions. Results of the conducted studies suggest that Th22 response may contribute to the skin and systemic inflammatory disease in psoriasis. It seems that early identification of soluble biomarkers and initiation of well-matched treatment may prevent exacerbation and progression of psoriasis.

scholarly journals Assessment of Serum and Tissue YKL-40 in Egyptian Psoriasis Vulgaris Patients versus Controls

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
M A Shaheen ◽  
K M Elzawahry ◽  
F W Georgy
Keyword(s):  
Disease Duration ◽  
Psoriasis Vulgaris ◽  
Inflammatory Diseases ◽  
Statistical Correlation ◽  
Tissue Levels ◽  
Pregnant Females ◽  
Egyptian Patients ◽  
Pasi Score ◽  
Elisa Technique ◽  
Control Study

Abstract Background Psoriasis is an inflammatory, papulosquamous disease of the skin. Serum YKL-40 is a useful biomarker of inflammation in psoriasis as well as other inflammatory diseases. Objectives To compare the levels of serum and tissue YKL-40 as a reliable biomarker of inflammation in psoriasis vulgaris patients versus controls. Patients and methods We performed a case-control study in 30 Egyptian patients with psoriasis vulgaris and 30 controls. Cases and controls were selected excluding pregnant females, individuals with any malignancies, patients who received systemic treatment for psoriasis for 1 month prior to their inclusion in the study and patients who received topical treatment for psoriasis 2 weeks prior to their inclusion in the study. The serum & tissue levels of YKL-40 were measured using ELISA technique. Results Both the serum and tissue levels of YKL-40 were elevated in psoriasis patients when compared with controls (p &lt; 0.01). These levels correlated highly with PASI score; both serum and tissue YKl-40 levels were significantly higher in patients with moderate psoriasis as compared with those with mild psoriasis. On the other hand, there was no statistical correlation between the serum and tissue levels of YKL-40 and age, BMI or disease duration. Conclusion The significant elevation in serum and tissue levels of YKl-40 in psoriatic patients when compared with healthy controls validates the marker in question as a reliable biomarker of inflammation in psoriasis vulgaris patients.

The Monoclonal Antibody-defined CAR-3 Antigen is a Serological Marker Associated with Pancreatic Carcinoma

1988 ◽  
Vol 3 (1) ◽  
pp. 29-35 ◽  
Author(s):  
M. Prat ◽  
E. Medico ◽  
P. Piantino ◽  
S. Bretti ◽  
F.P. Rossini ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Chronic Pancreatitis ◽  
Inflammatory Diseases ◽  
High Specificity ◽  
Serum Levels ◽  
Healthy Controls ◽  
Serum Concentrations ◽  
Breast Carcinomas ◽  
Chronic Inflammatory Diseases ◽  
Healthy Donors

The monoclonal antibody-defined CARS antigen is a new carcinoma associated marker which is expressed on a mucin-like molecule. Serum concentrations of CARS were assayed in 181 patients with carcinomas of different organs, 20 patients with non-carcinomatous malignancies, 123 patients with inflammatory diseases and 150 healthy controls. Serum levels of CARS were significantly increased in 51% of the patients with pancreatic carcinomas, in 60% of patients with biliary tract carcinomas and in about 15% of the patients with carcinomas of the digestive apparatus. Sera from patients with breast carcinomas were negative, as well as sera from patients with melanomas or sarcomas. CAR-3 values in samples from patients with chronic pancreatitis were constantly negative, as were samples from healthy donors. Significant concentrations of CAR-3 were detected in 20% of the sera from patients with acute pancreatitis and in 15% of the sera from patients with cirrhosis. Because of its high specificity for pancreatic carcinomas compared to chronic pancreatitis, CARS seems a promising marker for distinguishing between neoplastic and chronic inflammatory diseases of the pancreas, whose differential diagnosis is difficult.

scholarly journals C-Reactive Protein, Chemerin, Fetuin-A and Osteopontin as Predictors of Cardiovascular Risks in Persons With Psoriasis Vulgaris

2021 ◽  
pp. 383-391
Author(s):  
P BORSKY ◽  
Z FIALA ◽  
C ANDRYS ◽  
M BERANEK ◽  
K HAMAKOVA ◽  
...  
Keyword(s):  
Cardiovascular Diseases ◽  
Psoriasis Vulgaris ◽  
Serum Levels ◽  
Cardiovascular Risks ◽  
Fetuin A ◽  
Reactive Protein ◽  
Early Stages ◽  
Significant Relationships ◽  
Pasi Score

The study aimed to contribute to understanding the role of CRP, chemerin, fetuin-A and osteopontin and to assess their suitability as biomarkers of early stages of cardiovascular diseases in psoriasis vulgaris. Serum levels measured in 28 patients and 22 controls. Patients: increased levels of CRP (p<0.001), chemerin (p<0.05), osteopontin (p<0.05) and decreased levels of fetuin-A (p<0.05), significant relationships between CRP and fetuin-A (rho=0.530, p<0.01), CRP and chemerin (rho=0.543, p<0.01), CRP and age (rho=0.590, p<0.001), osteopontin and fetuin-A (r=-0.415, p<0.05), chemerin and PASI score (rho=-0.424, p<0.05). We confirmed specific roles of the biomarkers in psoriasis. CRP, fetuin-A and osteopontin could be considered appropriate markers for the detection of early stages of cardiovascular diseases.

scholarly journals The influence of atopy on sICAM-1 serum levels in patients with allergic rhinitis and bronchial asthma

2004 ◽  
Vol 23 (4) ◽  
pp. 355-359
Author(s):  
Aleksandra Peric-Popadic ◽  
Mirjana Bogic ◽  
Zikica Jovicic ◽  
Sanvila Raskovic ◽  
Vesna Tomic-Spiric ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Bronchial Asthma ◽  
Inflammatory Diseases ◽  
Serum Levels ◽  
Healthy Controls ◽  
Atopic Status ◽  
Significant Difference ◽  
Healthy Control ◽  
Adhesive Molecules ◽  
Important Marker

It has been shown that adhesive molecules are involved in inflammatory diseases of the lungs such as bronchial asthma. The purpose of the study was to measure and establish possible difference in serum levels of soluble ICAM-1 in 42 atopic patients (patients with allergic rhinitis and patients with bronchial asthma) in comparison with 28 patients without atopy (patients with asthma without rhinitis); whether there is a difference in sICAM-1 levels between groups of 26 patients with allergic rhinitis and asthma in comparison with group of 16 patients with allergic rhinitis only and also in comparison with 10 healthy controls. Results of the study have substantiated statistically significant difference in sICAM-1 levels between all groups of patients in comparison to healthy control, but no statistically significant difference in sICAM-1 levels between patients with and without atopy (Z=-1.738) or between patients with allergic rhinitis and bronchial asthma in comparison with group of patients with allergic rhinitis only (Z=0.00). ICAM-1 is an important marker of inflammation in patients with allergic rhinitis as well as in those with bronchial asthma. Atopic status does not influence differences in sICAM-1 levels. Although mean sICAM-1 levels were higher in patients with allergic rhinitis and bronchial asthma (312.71 ng/mL) in comparison with mean sICAM-1 levels in patients with allergic rhinitis only (279.69 ng/mL), no statistically significant difference was noted in sICAM-1 levels between these groups of subjects, i.e. asthma itself did not contribute to statistically significant increase of sICAM-1 levels.

scholarly journals Serum HMGB1 Serves as a Novel Laboratory Indicator Reflecting Disease Activity and Treatment Response in Ankylosing Spondylitis Patients

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Chenqiong Wang ◽  
Ye Miao ◽  
Xuefen Wu ◽  
Yishu Huang ◽  
Mengchen Sun ◽  
...  
Keyword(s):  
Disease Activity ◽  
Inflammatory Diseases ◽  
High Mobility ◽  
Serum Levels ◽  
Inflammatory Factor ◽  
Healthy Controls ◽  
Hip Joints ◽  
Significant Difference ◽  
Autoimmune And Inflammatory Diseases

Objective. High mobility group box 1 (HMGB1) is a late inflammatory factor participating in the pathogenesis of various autoimmune and inflammatory diseases. In the current study, we analyzed the association between serum levels of HMGB1 and clinical features of AS patients before and during treatment.Methods. Serum HMGB1 was detected in 147 AS patients and 61 healthy controls using ELISA. We evaluated the association between HMGB1 and extra-articular manifestations as well as disease severity indices. Among these AS patients, 41 patients received close follow-up at 1, 3, and 6 months after treatment. This group comprised 25 patients treated with anti-TNF-αbiologics and 16 patients receiving oral NSAIDs plus sulfasalazine.Results. The serum HMGB1 of AS patients was significantly higher than in healthy controls and positively correlated with BASDAI, BASFI, ASDAS-ESR, ASDAS-CRP, ESR, and CRP, but not with HLA-B27, anterior uveitis, and recurrent diarrhea. There was no significant difference between patients with radiographic damage of hip joints and those without. We observed that serum HMGB1 paralleled disease activity after treatment.Conclusion. Serum level of HMGB1 is higher in AS patients, and to some extent, HMGB1 can reflect the activity of AS and be used as a laboratory indicator to reflect the therapeutic response.

THU0468 Adipokine Serum Levels in Patients with Early Knee Osteoarthritis with Metabolic Syndrome and Healthy Controls

2015 ◽  
Vol 74 (Suppl 2) ◽  
pp. 370.1-370
Author(s):  
I. Guzman ◽  
C. Lozada ◽  
I. Valerio ◽  
A. Sanchez ◽  
R. Espinosa ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Knee Osteoarthritis ◽  
Serum Levels ◽  
Healthy Controls
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