scholarly journals Lactobacillus paracasei KW3110 Suppresses Inflammatory Stress-Induced Premature Cellular Senescence of Human Retinal Pigment Epithelium Cells and Reduces Ocular Disorders in Healthy Humans

2020 ◽  
Vol 21 (14) ◽  
pp. 5091
Author(s):  
Takahiro Yamazaki ◽  
Hiroaki Suzuki ◽  
Sayuri Yamada ◽  
Konomi Ohshio ◽  
Miho Sugamata ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Tight Junction ◽  
Cell Damage ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Lactobacillus Paracasei ◽  
Inflammatory Stress ◽  
Healthy Humans ◽  
Eye Fatigue ◽  
Ocular Disorders

Lactobacillus paracasei KW3110 (KW3110) has anti-inflammatory effects and mitigates retinal pigment epithelium (RPE) cell damage caused by blue-light exposure. We investigated whether KW3110 suppresses chronic inflammatory stress-induced RPE cell damage by modulating immune cell activity and whether it improves ocular disorders in healthy humans. First, we showed that KW3110 treatment of mouse macrophages (J774A.1) produced significantly higher levels of interleukin-10 as compared with other lactic acid bacterium strains (all p < 0.01). Transferring supernatant from KW3110- and E. coli 0111:B4 strain and adenosine 5′-triphosphate (LPS/ATP)-stimulated J774A.1 cells to human retinal pigment epithelium (ARPE-19) cells suppressed senescence-associated phenotypes, including proliferation arrest, abnormal appearance, cell cycle arrest, and upregulation of cytokines, and also suppressed expression of tight junction molecule claudin-1. A randomized, double-blind, placebo-controlled parallel-group study of healthy subjects (n = 88; 35 to below 50 years) ingesting placebo or KW3110-containing supplements for 8 weeks showed that changes in critical flicker frequency, an indicator of eye fatigue, from the week-0 value were significantly larger in the KW3110 group at weeks 4 (p = 0.040) and 8 (p = 0.036). These results suggest that KW3110 protects ARPE-19 cells against premature senescence and aberrant expression of tight junction molecules caused by chronic inflammatory stress, and may improve chronic eye disorders including eye fatigue.

scholarly journals Differential day-night expression of tight junction components in murine retinal pigment epithelium

2020 ◽  
Vol 193 ◽  
pp. 107985 ◽  
Author(s):  
Elja M.M. Louer ◽  
Dorothee Günzel ◽  
Rita Rosenthal ◽  
Claudia Carmone ◽  
Guoqiang Yi ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Tight Junction ◽  
Pigment Epithelium ◽  
Retinal Pigment

Intracellular amyloid beta alters the tight junction of retinal pigment epithelium in 5XFAD mice

2014 ◽  
Vol 35 (9) ◽  
pp. 2013-2020 ◽  
Author(s):  
Sung Wook Park ◽  
Jin Hyoung Kim ◽  
Inhee Mook-Jung ◽  
Kyu-Won Kim ◽  
Woo Jin Park ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Tight Junction ◽  
Amyloid Beta ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
5Xfad Mice

MiR-340/iASPP axis affects UVB-mediated retinal pigment epithelium (RPE) cell damage

2018 ◽  
Vol 186 ◽  
pp. 9-16 ◽  
Author(s):  
Jiazhao Yan ◽  
Yuhui Qin ◽  
Jingsheng Yu ◽  
Qinghua Peng ◽  
Xiangdong Chen
Keyword(s):  
Retinal Pigment Epithelium ◽  
Cell Damage ◽  
Pigment Epithelium ◽  
Retinal Pigment

Human retinal pigment epithelium expressed tight junction markers in spheroid cultures

2009 ◽  
Vol 87 ◽  
pp. 0-0
Author(s):  
I PINILLA ◽  
LS WRIGHT ◽  
E GARCIA MARTIN ◽  
DM GAMM ◽  
NICOLAS CUENCA
Keyword(s):  
Retinal Pigment Epithelium ◽  
Tight Junction ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Human Retinal Pigment Epithelium

scholarly journals The Tight Junction Associated Signalling Proteins ZO-1 and ZONAB Regulate Retinal Pigment Epithelium Homeostasis in Mice

PLoS ONE ◽  
2010 ◽  
Vol 5 (12) ◽  
pp. e15730 ◽  
Author(s):  
Anastasios Georgiadis ◽  
Marion Tschernutter ◽  
James W. B. Bainbridge ◽  
Kamaljit S. Balaggan ◽  
Freya Mowat ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Tight Junction ◽  
Pigment Epithelium ◽  
Retinal Pigment

Immortalization of polarized rat retinal pigment epithelium

1993 ◽  
Vol 104 (1) ◽  
pp. 37-49 ◽  
Author(s):  
I.R. Nabi ◽  
A.P. Mathews ◽  
L. Cohen-Gould ◽  
D. Gundersen ◽  
E. Rodriguez-Boulan
Keyword(s):  
Retinal Pigment Epithelium ◽  
Tight Junction ◽  
Cell Line ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Primary Cultures ◽  
Neural Retina ◽  
Permissive Temperature ◽  
Apical Surface ◽  
Junction Protein

Rat retinal pigment epithelial (RPE) cells were immortalized by infection with a temperature-sensitive tsA SV40 virus and following cloning and selection for epithelial properties the polarized RPE-J cell line was obtained. At the permissive temperature of 33 degrees C, RPE-J cells behave as an immortalized cell line. When RPE-J cells are grown on nitrocellulose filters coated with a thin layer of Matrigel in the presence of 10(−8) M retinoic acid for 6 days at 33 degrees C and then switched for 33–36 hours to the non-permissive temperature of 40 degrees C, they acquire a differentiated polarized RPE phenotype. Under these growth conditions, RPE-J cells exhibit circumferential staining for the tight-junction protein ZO-1 and acquire a transepithelial resistance of 350 ohms cm2. Morphologically, RPE-J cells exhibit a characteristic RPE morphology with extensive apical microvilli as well as numerous dense bodies including premelanosomes and varied multilamellar structures. Ruthenium red labeling revealed the frequent basal localization of the tight junction. The cells were identified to be of rat RPE origin by their expression of the rat RPE marker RET-PE2 and their ability to phagocytose latex beads. While RPE-J cells are capable of sorting influenza and vesicular stomatitis virus to the apical and basal surfaces, respectively, the Na,K-ATPase is not polarized and the neural cell adhesion molecule, N-CAM, is localized exclusively to the lateral surface. In vivo the apical surface of RPE interacts with the adjacent neural retina and the Na,K-ATPase and N-CAM are both apical; the altered polarity of these two proteins in RPE-J cells may be a consequence of the absence of apical interaction with the neural retina in culture. Previous studies of RPE have been restricted to the use of primary cultures and the RPE-J cell line should prove an excellent model system for the study of the mechanisms determining the characteristic polarity and functions of the retinal pigment epithelium.

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