scholarly journals Quantitative Trait Locus Mapping of Clubroot Resistance and Plasmodiophora brassicae Pathotype Banglim-Specific Marker Development in Brassica rapa

2020 ◽  
Vol 21 (11) ◽  
pp. 4157
Author(s):  
Su Ryun Choi ◽  
Sang Heon Oh ◽  
Sushil Satish Chhapekar ◽  
Vignesh Dhandapani ◽  
Chang Yeol Lee ◽  
...  
Keyword(s):  
Double Haploid ◽  
Quantitative Trait ◽  
Plasmodiophora Brassicae ◽  
Gene Pyramiding ◽  
Dominant Gene ◽  
Double Haploid Population ◽  
Specific Marker ◽  
Phenotypic Variance ◽  
Clubroot Resistance ◽  
Haploid Population

Clubroot resistance is an economically important trait in Brassicaceae crops. Although many quantitative trait loci (QTLs) for clubroot resistance have been identified in Brassica, disease-related damage continues to occur owing to differences in host variety and constant pathogen variation. Here, we investigated the inheritance of clubroot resistance in a double haploid population developed by crossing clubroot resistant and susceptible lines “09CR500” and “09CR501”, respectively. The resistance of “09CR500” to Plasmodiophora brassicae pathotype “Banglim” was controlled as a single dominant gene, with the segregation of resistance and susceptibility being nearly 1:1. PbBrA08Banglim was identified as having a logarithm of odds value of 7.9–74.8, and a phenotypic variance of 26.0–97.1% with flanking marker “09CR.11390652” in A08. After aligning QTL regions to the B. rapa reference genome, 11 genes were selected as candidates. PbBrA08Banglim was located near Crr1, CRs, and Rcr9 loci, but differences were validated by marker analysis, gene structural variations, and gene expression levels, as well as phenotypic responses to the pathotype. Genotyping using the “09CR.11390652” marker accurately distinguished the Banglim-resistance phenotypes in the double haploid population. Thus, the developed marker will be useful in Brassica breeding programs, marker-assisted selection, and gene pyramiding to identify and develop resistant cultivars.

Mapping quantitative trait loci for sheath blight resistance in rice using double haploid population

2010 ◽  
Vol 130 (3) ◽  
pp. 404-406 ◽  
Author(s):  
Qun Xu ◽  
XiaoPing Yuan ◽  
HanYong Yu ◽  
YiPing Wang ◽  
ShengXiang Tang ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Double Haploid ◽  
Quantitative Trait ◽  
Sheath Blight ◽  
Double Haploid Population ◽  
Blight Resistance ◽  
Trait Loci ◽  
Haploid Population ◽  
Sheath Blight Resistance

Microsatellite markers linked to QTL for resistance to Mal de Río Cuarto disease in Zea mays L.

2004 ◽  
Vol 142 (3) ◽  
pp. 289-295 ◽  
Author(s):  
M. A. DI RENZO ◽  
N. C. BONAMICO ◽  
D. G. DÍAZ ◽  
M. A. IBAÑEZ ◽  
M. E. FARICELLI ◽  
...  
Keyword(s):  
Zea Mays ◽  
Inbred Line ◽  
Quantitative Trait ◽  
Dominant Gene ◽  
Phenotypic Selection ◽  
Severity Index ◽  
Viral Disease ◽  
Interval Mapping ◽  
Phenotypic Variance ◽  
Ssr Loci

‘Mal de Río Cuarto’ (MRC) disease, caused by a member of the family Reoviridae belonging to the genus Fijivirus, is considered to be the most damaging viral disease of maize (Zea mays L.) in Argentina. Resistance to MRC disease is a quantitative trait with moderate heritability ranging from 0·44 to 0·56. The objective of this study was to identify simple sequence repeats (SSR) loci linked to quantitative trait loci (QTL) contributing to MRC disease resistance. Two hundred and twenty-seven F3 derived-lines from a cross between a susceptible inbred line, Mo17, and a partially resistant inbred line, BLS14, were evaluated across four Río Cuarto environments. A disease severity index (DSI) based on disease grades was calculated and used to rate F3 derived-lines for their resistance to MRC disease. A subset of parental F2 plants belonging to susceptible and resistant F3 derived-lines from field assessments was assayed for 180 SSR primer pairs to map resistance genes. Fifty-six maize SSR were employed for the testing of linkage among DNA markers and the mapping of QTL through composite interval mapping. Resistance to MRC disease was affected by two QTL on chromosomes 1 and 8 which showed overdominance and dominant gene action, respectively. A simultaneous fit with these QTL in the joint analyses explained 36·2% of the phenotypic variance. In spite of the fact that relative efficiency of marker-assisted selection (MAS) in comparison to phenotypic selection was close to 1, the mapped QTL could improve the efficiency of efforts in breeding for resistance to MRC disease.

Grain Yield Potential Strategies in an Elite Wheat Double-Haploid Population Grown in Contrasting Environments

Crop Science ◽  
2013 ◽  
Vol 53 (6) ◽  
pp. 2577-2587 ◽  
Author(s):  
Guillermo A. García ◽  
Ahmed K. Hasan ◽  
Laura E. Puhl ◽  
Matthew P. Reynolds ◽  
Daniel F. Calderini ◽  
...  
Keyword(s):  
Grain Yield ◽  
Double Haploid ◽  
Double Haploid Population ◽  
Yield Potential ◽  
Haploid Population

scholarly journals Mapping Quantitative Trait Loci for 1000-Grain Weight in a Double Haploid Population of Common Wheat

2020 ◽  
Vol 21 (11) ◽  
pp. 3960 ◽  
Author(s):  
Tao Liu ◽  
Lijun Wu ◽  
Xiaolong Gan ◽  
Wenjie Chen ◽  
Baolong Liu ◽  
...  
Keyword(s):  
Quantitative Trait ◽  
Genetic Distances ◽  
Snp Markers ◽  
Grain Weight ◽  
High Yield ◽  
Doubled Haploid Population ◽  
Phenotypic Variance ◽  
Yield Trait ◽  
Haploid Population ◽  
Trait Locus

Thousand-grain weight (TGW) is a very important yield trait of crops. In the present study, we performed quantitative trait locus (QTL) analysis of TGW in a doubled haploid population obtained from a cross between the bread wheat cultivar “Superb” and the breeding line “M321” using the wheat 55-k single-nucleotide polymorphism (SNP) genotyping assay. A genetic map containing 15,001 SNP markers spanning 2209.64 cM was constructed, and 9 QTLs were mapped to chromosomes 1A, 2D, 4B, 4D, 5A, 5D, 6A, and 6D based on analyses conducted in six experimental environments during 2015–2017. The effects of the QTLs qTgw.nwipb-4DS and qTgw.nwipb-6AL were shown to be strong and stable in different environments, explaining 15.31–32.43% and 21.34–29.46% of the observed phenotypic variance, and they were mapped within genetic distances of 2.609 cM and 5.256 cM, respectively. These novel QTLs may be used in marker-assisted selection in wheat high-yield breeding.

scholarly journals Identification of Candidate Genes for Clubroot-Resistance in Brassica oleracea Using Quantitative Trait Loci-Sequencing

2021 ◽  
Vol 12 ◽  
Author(s):  
Fuquan Ce ◽  
Jiaqin Mei ◽  
Haiyan He ◽  
Yu Zhao ◽  
Wenhui Hu ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Brassica Oleracea ◽  
Candidate Genes ◽  
Quantitative Trait ◽  
Plasmodiophora Brassicae ◽  
Potential Candidate ◽  
Clubroot Resistance ◽  
Group I ◽  
Trait Loci ◽  
Group Ii

Clubroot caused by Plasmodiophora brassicae is a devastating disease of cabbage (Brassica oleracea). To identify quantitative trait loci (QTLs) for clubroot resistance (CR) in B. oleracea, genomic resequencing was carried out in two sets of extreme pools, group I and group II, which were constructed separately from 110 and 74 F2 cloned lines derived from the cross between clubroot-resistant (R) cabbage “GZ87” (against race 4) and susceptible (S) cabbage “263.” Based on the QTL-sequencing (QTL-Seq) analysis of group I and group II, three QTLs (i.e., qCRc7-2, qCRc7-3, and qCRc7-4) were determined on the C07 chromosome. RNA-Seq and qRT-PCR were conducted in the extreme pools of group II before and after inoculation, and two potential candidate genes (i.e., Bol037115 and Bol042270), which exhibiting upregulation after inoculation in the R pool but downregulation in the S pool, were identified from the three QTLs on C07. A functional marker “SWU-OA” was developed from qCRc7-4 on C07, exhibiting ∼95% accuracy in identifying CR in 56 F2 lines. Our study will provide valuable information on resistance genes against P. brassicae and may accelerate the breeding process of B. oleracea with CR.

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