scholarly journals OsCpn60β1 is Essential for Chloroplast Development in Rice (Oryza sativa L.)

2020 ◽  
Vol 21 (11) ◽  
pp. 4023
Author(s):  
Qingfei Wu ◽  
Cheng Zhang ◽  
Yue Chen ◽  
Kaiyue Zhou ◽  
Yihua Zhan ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Oryza Sativa ◽  
Chloroplast Development ◽  
Oryza Sativa L ◽  
Rice Genome ◽  
Targeted Mutagenesis ◽  
Label Free ◽  
Microscopy Observation ◽  
Leaf Phenotype ◽  
Chaperonin 60

The chaperonin 60 (Cpn60) protein is of great importance to plants due to its involvement in modulating the folding of numerous chloroplast protein polypeptides. In chloroplasts, Cpn60 is differentiated into two subunit types—Cpn60α and Cpn60β and the rice genome encodes three α and three β plastid chaperonin subunits. However, the functions of Cpn60 family members in rice were poorly understood. In order to investigate the molecular mechanism of OsCpn60β1, we attempted to disrupt the OsCpn60β1 gene by CRISPR/Cas9-mediated targeted mutagenesis in this study. We succeeded in the production of homozygous OsCpn60β1 knockout rice plants. The OsCpn60β1 mutant displayed a striking albino leaf phenotype and was seedling lethal. Electron microscopy observation demonstrated that chloroplasts were severely disrupted in the OsCpn60β1 mutant. In addition, OsCpn60β1 was located in the chloroplast and OsCpn60β1 is constitutively expressed in various tissues particularly in the green tissues. The label-free qualitative proteomics showed that photosynthesis-related pathways and ribosomal pathways were significantly inhibited in OsCpn60β1 mutants. These results indicate that OsCpn60β1 is essential for chloroplast development in rice.

Identification and chromosomal localization of a transcriptionally active retrotransposon of Ty3-gypsy type in rice

Genome ◽  
2000 ◽  
Vol 43 (2) ◽  
pp. 404-408 ◽  
Author(s):  
Zi-Yin Li ◽  
Shou-Yi Chen ◽  
Xian-Wu Zheng ◽  
Li-Huang Zhu
Keyword(s):  
Oryza Sativa ◽  
Copy Number ◽  
Oryza Sativa L ◽  
Blot Hybridization ◽  
Rice Genome ◽  
Rnase H ◽  
Northern Blot Hybridization ◽  
Japonica Rice ◽  
Terminal Part ◽  
Dot Blot

A DNA fragment representing a transcriptionally active retrotransposon of Ty3-gypsy type was isolated and characterized from rice (Oryza sativa L.). The fragment (named RIRE9) includes the coding sequences for the C-terminal part of the RNase H domain and the N-terminal part of the integrase domain in the polyprotein region. Northern blot hybridization indicated that this element was expressed in rice leaves and stems, suggesting that it is potentially active to transpose under normal growth conditions. Using dot-blot hybridization, the copy number of RIRE9 was estimated to be about 1600 copies per haploid rice genome. Five homologous copies of RIRE9 were assigned to five distinct positions of four chromosomes by restriction fragment length polymorphism (RFLP) mapping approach using an indica-japonica rice doubled-haploid (DH) population and its molecular linkage map. Key words: Oryza sativa L., Ty3-gypsy-like retrotransposon, copy number, chromosomal location.

scholarly journals Genetics of Hybrid Sterility and Hybrid Breakdown in an Intersubspecific Rice (Oryza sativa L.) Population

Genetics ◽  
1997 ◽  
Vol 145 (4) ◽  
pp. 1139-1148 ◽  
Author(s):  
Zhikang Li ◽  
Shannon R M Pinson ◽  
Andrew H Paterson ◽  
William D Park ◽  
James W Stansel
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Hybrid Sterility ◽  
Rice Genome ◽  
F1 Hybrids ◽  
Progeny Testing ◽  
Hybrid Breakdown ◽  
F1 Hybrid ◽  
Genetic Mechanisms ◽  
Complicated Nature

F1 hybrid sterility and “hybrid breakdown” of F2 and later generations in rice (Oryza sativa L.) are common and genetically complicated. We used a restriction fragment length polymorphism linkage map and F4 progeny testing to investigate hybrid sterility and hybrid breakdown in a cross between “widely compatible” O. sativa ssp. japonica cultivar Lemont from the Southern U.S. and ssp. indica cultivar Teqing from China. Our results implicate different genetic mechanisms in hybrid sterility and hybrid breakdown, respectively. Hybrid sterility appeared to be due to recombination within a number of putative differentiated “supergenes” in the rice genome, which may reflect cryptic structural rearrangements. The cytoplasmic genome had a large effect on fertility of both male and female gametes in the F1 hybrids. There appeared to be a pair of complementary genes that behaved like “wide compatibility” genes. This pair of genes and the “gamete eliminator” (S1) or “egg killer” (S-5) may influence the phenotypic effects of presumed supergenes in hybrids. Hybrid breakdown appeared to be largely due to incompatibilities between indica and japonica alleles at many unlinked epistatic loci in the genome. These proposed mechanisms may partly account for the complicated nature of postreproductive barriers in rice.

CRISPR/Cas9-targeted mutagenesis of F3′H, DFR and LDOX, genes related to anthocyanin biosynthesis in black rice (Oryza sativa L.)

2019 ◽  
Vol 13 (5) ◽  
pp. 521-531 ◽  
Author(s):  
Yu Jin Jung ◽  
Hyo Ju Lee ◽  
Jong Hee Kim ◽  
Dong Hyen Kim ◽  
Hee Kyoung Kim ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Anthocyanin Biosynthesis ◽  
Oryza Sativa L ◽  
Targeted Mutagenesis ◽  
Black Rice
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