scholarly journals Beneficial Effects of Inflammatory Cytokine-Targeting Aptamers in an Animal Model of Chronic Prostatitis

2020 ◽  
Vol 21 (11) ◽  
pp. 3953
Author(s):  
Dong-Ru Ho ◽  
Pey-Jium Chang ◽  
Wei-Yu Lin ◽  
Yun-Ching Huang ◽  
Jian-Hui Lin ◽  
...  
Keyword(s):  
Inflammatory Cytokine ◽  
Interleukin 1 ◽  
Chronic Pelvic Pain Syndrome ◽  
Pain Syndrome ◽  
B Cell Lymphoma ◽  
Pelvic Pain Syndrome ◽  
Beneficial Effects ◽  
Carrageenan Injection ◽  
Prostate Inflammation

Non-bacterial prostatitis is an inflammatory disease that is difficult to treat. Oligonucleotide aptamers are well known for their stability and flexibility in conjugating various inflammatory molecules. In this study, we investigated the effects of inflammatory cytokine-targeting aptamers (ICTA), putative neutralizers of TNF-alpha and IL-1 beta activation, on local carrageenan-induced prostate inflammation, allodynia, and hyperalgesia in rats. In vitro evaluation confirmed the binding capability of ICTA. Intraprostatic injection of carrageenan or control vehicle was performed in six-week-old rats, and ICTA (150 µg) or vehicle was administered in the prostate along with carrageenan injection. The von Frey filament test was performed to determine mechanical allodynia, and prostate inflammation was examined seven days after drug administration. Local carrageenan administration resulted in a reduction of the tactile threshold. The levels of mononuclear cell infiltration, pro-inflammatory cytokine interleukin-1 beta (b), caspase-1 (casp-1), and Nucleotide-binding oligomerization domain, Leucine rich Repeat and Pyrin domain containing proteins 1 and 3 (NALP1 and NALP3) in the prostate of rats were increased seven days after carrageenan injection. Treatment with ICTA significantly attenuated the carrageenan-induced hyperalgesia and reduced the elevated levels of proteins including TNF-a and IL-1b in the rats. Apoptosis markers, B-cell lymphoma 2-associated X protein (Bax) and caspase-3, were elevated in ICTA-treated Chronic pelvic pain syndrome (CPPS) rats. These results suggest that ICTA provides protection against local carrageenan-induced enhanced pain sensitivity, and that the neutralization of proinflammatory cytokines may result in inflammatory cell apoptosis.

scholarly journals Search for Novel Diagnostic Biomarkers of Prostate Inflammation-Related Disorders: Role of Transglutaminase Isoforms as Potential Candidates

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Maria Pia Savoca ◽  
Antonino Inferrera ◽  
Elisabetta A. M. Verderio ◽  
Daniela Caccamo
Keyword(s):  
Posttranslational Modifications ◽  
Chronic Pelvic Pain Syndrome ◽  
Pain Syndrome ◽  
Pelvic Pain Syndrome ◽  
Prostate Hyperplasia ◽  
Diagnostic Biomarkers ◽  
Pathological Conditions ◽  
Prostate Inflammation ◽  
Literature Evidence ◽  
Noninvasive Biomarkers

Investigations on prostate inflammation-related disorders, including acute and chronic prostatitis, chronic pelvic pain syndrome, benign prostate hyperplasia (BPH), and prostate cancer (PCa), are still ongoing to find new, accurate, and noninvasive biomarkers for a differential diagnosis of those pathological conditions sharing some common macroscopic features. Moreover, an ideal biomarker should be useful for risk assessment of prostate inflammation progression to more severe disorders, like BPH or PCa, as well as for monitoring of treatment response and prognosis establishment in carcinoma cases. Recent literature evidence highlighted that changes in the expression of transglutaminases, enzymes that catalyze transamidation reactions leading to posttranslational modifications of soluble proteins, occur in prostate inflammation-related disorders. This review focuses on the role specifically played by transglutaminases 4 (TG4) and 2 (TG2) and suggests that both isoenzymes hold a potential to be included in the list of candidates as novel diagnostic biomarkers for the above-cited prostate pathological conditions.

Influence of myofascial therapy on painful erection – case study

Ból ◽  
2020 ◽  
Vol 21 (3) ◽  
pp. 1-6
Author(s):  
Adrian Rogala ◽  
Grażyna Brzuszkiewicz-Kuźmicka
Keyword(s):  
Pain Relief ◽  
Connective Tissue ◽  
Muscle Tone ◽  
Chronic Pelvic Pain Syndrome ◽  
Pain Syndrome ◽  
Trigger Points ◽  
Pelvic Pain Syndrome ◽  
Beneficial Effects ◽  
The Many

Pain during sexual intercourse in men is a well-recognized symptom that is considered in the context of sexual dysfunction. Because there is no clear definition, it can be a joint feature of chronic prostatitis, chronic pelvic pain syndrome (CPPS), or come from connective tissue. Because of the many possible causes of genitourinary pain, a multimodal approach is necessary. The aim of this study is to present the effect of myofascial therapy on chronic, unpleasant, dull pain at the level of VAS = 9, occurring during erection and ejaculation, as well as during touch of the testicle in a 35-year-old man. The patient did not have erection problems, which was confirmed by an accurate and reliable tool in the form of the International Index of Erectile Function (IIEF-5). The man received two myofascial therapies, 45 min. each, 7 days apart followed by significant improvement in pain relief (VAS = 0). The manual techniques were used to find and remove changes manifested by increased muscle tone and fascia, lack of displacement of structures relative to each other, maximum painful points, and trigger points. In the follow-up examination performed after 1, 3 and 6 months, the patient confirmed that the effects of therapy were maintained. Connective tissue may play an important role in the occurrence of genitourinary pain. Physiotherapeutic examination and usage of myofascial techniques can bring beneficial effects in the form of pain relief in the relevant region.

scholarly journals IL-1β-primed mesenchymal stromal cells exert enhanced therapeutic effects to alleviate Chronic Prostatitis/Chronic Pelvic Pain Syndrome through systemic immunity

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Hanchao Liu ◽  
Xinning Zhu ◽  
Xiaohui Cao ◽  
Ani Chi ◽  
Jian Dai ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Chronic Prostatitis ◽  
Chronic Pelvic Pain ◽  
Chronic Pelvic Pain Syndrome ◽  
Pain Syndrome ◽  
Prostate Tissue ◽  
Pelvic Pain Syndrome ◽  
Therapeutic Effects ◽  
Systemic Immunity

Abstract Background Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) seriously affects patient health. Despite the elusiveness of innate therapeutic effects, mesenchymal stromal cells (MSCs) hold great promise for inflammation-related diseases. Recent evidence indicates that disease-specific inflammatory cytokines could enhance the therapeutic effects of MSCs. Methods By establishing a CP/CPPS mouse model and pretreating MSCs with the cytokine interleukin-1β (IL-1β), we studied the IL-1β-primed MSC immunoregulatory ability and targeted migration ability in vitro and in CP/CPPS mice. Results IL-1β levels significantly increased in the prostate tissue and serum of experimental autoimmune prostatitis (EAP) mice. Pretreatment with IL-1β enhanced the immunomodulatory potential and targeted migration of MSCs in vitro. Furthermore, intravenous infusion of IL-1β-primed MSCs dampened inflammation in prostate tissues and alleviated hyperalgesia in EAP mice. The infused MSCs inhibited monocyte infiltration and promoted regulatory T lymphocyte formation in prostate tissue, thus remodeling the local environment. Surprisingly, IL-1β-primed MSCs exhibited improved accumulation in the spleen but not in prostate tissue. Accordingly, infused MSCs reshaped systemic immunity by reducing the proportion of Ly6ChighCD11b+ monocytes and boosting the proportion of CD4+Foxp3+ regulatory T lymphocytes in the spleen and lung. Inflammatory chemokine (C–C motif) ligand 2 (CCL2) decreased through the downregulation of the NF-κB and JNK/MAPK pathways by inflammatory resolution via MSCs infusion to alleviate pain. Conclusion In summary, IL-1β-primed MSCs restored systemic immunologic homeostasis to alleviate CP/CPPS by modulating systemic immunity. These findings provide a novel strategy to boost the therapeutic effects of MSC-based therapy for CP/CPPS and reveal the essential role of systematic immunity in the treatment of CP/CPPS with MSC infusion.

scholarly journals Complementary and Alternative Medicine for Chronic Prostatitis/Chronic Pelvic Pain Syndrome

2005 ◽  
Vol 2 (4) ◽  
pp. 495-501 ◽  
Author(s):  
Jillian L. Capodice ◽  
Debra L. Bemis ◽  
Ralph Buttyan ◽  
Steven A. Kaplan ◽  
Aaron E. Katz
Keyword(s):  
Pelvic Pain ◽  
Chronic Prostatitis ◽  
Chronic Pelvic Pain ◽  
Treatment Options ◽  
Chronic Pelvic Pain Syndrome ◽  
Pain Syndrome ◽  
Pelvic Pain Syndrome ◽  
Complementary And Alternative

To discuss challenges concerning treatment for chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) and review complementary and alternative medical (CAM) therapies being evaluated for this condition, we performed a comprehensive search of articles published from 1990–2005 using the PubMed, Medline databases. Data from the articles were abstracted and pooled by subject. Keywords cross-searched with CP/CPPS included: complementary, alternative, integrative, therapies, interventions, nutrition, antioxidants, herbs, supplements, biofeedback and acupuncture. Listed articles with no abstracts were not included. Various CAM therapies for CP/CPPS exist including biofeedback, acupuncture, hyperthermia and electrostimulation. Additionally, a variety ofin vitroandin vivostudies testing herbal and nutritional supplements were found. Saw palmetto, cernilton and quercetin were the most frequently tested supplements for CP/CPP Although many CAM therapies demonstrate positive preliminary observations as prospective treatments for CP/CPPS, further exploratory studies including more randomized, controlled trials are necessary for significant validation as treatment options for this complex disorder.

95: Incidence and Significance of Prostatic Stones in Men with Chronic Prostatitis/Chronic Pelvic Pain Syndrome

2007 ◽  
Vol 177 (4S) ◽  
pp. 33-34
Author(s):  
Daniel A. Shoskes ◽  
Chun-Te Lee ◽  
Donel Murphy ◽  
John C. Kefer ◽  
Hadley M. Wood
Keyword(s):  
Pelvic Pain ◽  
Chronic Prostatitis ◽  
Chronic Pelvic Pain ◽  
Chronic Pelvic Pain Syndrome ◽  
Pain Syndrome ◽  
Pelvic Pain Syndrome

87: Biopsychosocial Factors in Quality of Life in men with Chronic Prostatitis/Chronic Pelvic Pain Syndrome

2007 ◽  
Vol 177 (4S) ◽  
pp. 31-31
Author(s):  
J. Curtis Nickel ◽  
Dean Tripp ◽  
Shannon Chuai ◽  
Mark S. Litwin ◽  
Mary McNaughton-Collins
Keyword(s):  
Quality Of Life ◽  
Pelvic Pain ◽  
Chronic Prostatitis ◽  
Chronic Pelvic Pain ◽  
Chronic Pelvic Pain Syndrome ◽  
Pain Syndrome ◽  
Pelvic Pain Syndrome
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