scholarly journals Devitalizing Effect of High Hydrostatic Pressure on Human Cells—Influence on Cell Death in Osteoblasts and Chondrocytes

2020 ◽  
Vol 21 (11) ◽  
pp. 3836
Author(s):  
Janine Waletzko ◽  
Michael Dau ◽  
Anika Seyfarth ◽  
Armin Springer ◽  
Marcus Frank ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Cell Death ◽  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Cell Damage ◽  
Water Soluble ◽  
Cartilage Tissue ◽  
Initial Assessment ◽  
Tetrazolium Salt ◽  
Tissue Samples

Chemical and physical processing of allografts is associated with a significant reduction in biomechanics. Therefore, treatment of tissue with high hydrostatic pressure (HHP) offers the possibility to devitalize tissue gently without changing biomechanical properties. To obtain an initial assessment of the effectiveness of HHP treatment, human osteoblasts and chondrocytes were treated with different HHPs (100–150 MPa, 250–300 MPa, 450–500 MPa). Devitalization efficiency was determined by analyzing the metabolic activity via WST-1(water-soluble tetrazolium salt) assay. The type of cell death was detected with an apoptosis/necrosis ELISA (enzyme-linked immune sorbent assay) and flow cytometry. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) were carried out to detect the degree of cell destruction. After HHP treatment, the metabolic activities of both cell types decreased, whereas HHP of 250 MPa and higher resulted in metabolic inactivation. Further, the highest HHP range induced mostly necrosis while the lower HHP ranges induced apoptosis and necrosis equally. FESEM and TEM analyses of treated osteoblasts revealed pressure-dependent cell damage. In the present study, it could be proven that a pressure range of 250–300 MPa can be used for cell devitalization. However, in order to treat bone and cartilage tissue gently with HHP, the results of our cell experiments must be verified for tissue samples in future studies.

scholarly journals Caspase-2 and oxidative stress underlie the immunogenic potential of high hydrostatic pressure-induced cancer cell death

2016 ◽  
Vol 6 (1) ◽  
pp. e1258505 ◽  
Author(s):  
Irena Moserova ◽  
Iva Truxova ◽  
Abhishek D. Garg ◽  
Jakub Tomala ◽  
Patrizia Agostinis ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Hydrostatic Pressure ◽  
Cancer Cell ◽  
High Hydrostatic Pressure ◽  
Cancer Cell Death ◽  
Caspase 2 ◽  
And Oxidative Stress

scholarly journals Влияние температуры деформации в камере Бриджмена на особенности формирования дефектной структуры технически чистого титана

2018 ◽  
Vol 44 (20) ◽  
pp. 70
Author(s):  
Н.А. Шурыгина ◽  
А.М. Глезер ◽  
Д.Л. Дьяконов ◽  
А.А. Томчук ◽  
А.Г. Кадомцев ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Defect Structures ◽  
Cryogenic Temperatures ◽  
Two Phase ◽  
Α Phase ◽  
Electron Microscopy Data ◽  
Phase Mixture ◽  
Microscopy Data

AbstractTransmission electron microscopy data showed evidence of the formation of structural regions corresponding to deformation (dislocated) fragments and dynamically recrystallized grains in α-phase titanium upon torsion at high hydrostatic pressure at room and cryogenic temperatures. It is shown that the previously proposed “two-phase mixture” model is applicable to description of these defect structures.

The effects of high hydrostatic pressure on microfilaments and microtubules in Xenopus laevis

Development ◽  
1978 ◽  
Vol 44 (1) ◽  
pp. 281-295
Author(s):  
Paul-Emil Messier ◽  
C. Seguin
Keyword(s):  
Electron Microscopy ◽  
Hydrostatic Pressure ◽  
Xenopus Laevis ◽  
Cell Elongation ◽  
High Hydrostatic Pressure ◽  
Light And Electron Microscopy ◽  
Duration Of Treatment ◽  
Neuroepithelial Cells ◽  
Exerted Pressure ◽  
Xenopus Laevis Embryos

Xenopus laevis embryos of stages 14–20 were subjected, for periods of 5–330 min, to hydrostatic pressures ranging from 500 to 10000 psi. The specimens were fixed under corresponding pressures and their neuroepithelium was studied under light and electron microscopy. A pressure of 3000 psi, maintained for as long as 180 min, did not inhibit neurulation though it induced slight deformities of the neuroepithelium. A pressure of 4000 psi, applied for 180 min, disrupted the apical ring of microfilaments and blocked neurulation. The cells lost their dissymmetry. The effect was reversible. Lengthening the duration of treatment to 330 min caused the neuroepithelial cells to loose their microtubules and to become round. This situation was not reversible. Our results indicated that microfilaments are more sensitive than microtubules, that both organelles became increasingly sensitive as the exerted pressure was increased and that microtubules of older embryos exhibited a better resistance. Finally, we showed a correlation between the presence of microfilaments and the constricted state of the cellular apices and a relationship between the presence of microtubules and cell elongation.

scholarly journals A Novel Proteomic Analysis of the Modifications Induced by High Hydrostatic Pressure on Hazelnut Water-Soluble Proteins

Foods ◽  
2014 ◽  
Vol 3 (2) ◽  
pp. 279-289 ◽  
Author(s):  
Nuria Prieto ◽  
Carmen Burbano ◽  
Elisa Iniesto ◽  
Julia Rodríguez ◽  
Beatriz Cabanillas ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Proteomic Analysis ◽  
Soluble Proteins ◽  
Water Soluble

Molecular characterization of immunogenic cell death triggered by the high hydrostatic pressure in human tumor cells.

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. e14008-e14008
Author(s):  
Irena Moserova ◽  
Iva Truxova ◽  
Pierre-Francois Cartron ◽  
Jirina Bartunkova ◽  
Radek Spisek ◽  
...  
Keyword(s):  
Cell Death ◽  
Hydrostatic Pressure ◽  
Tumor Cells ◽  
Molecular Characterization ◽  
High Hydrostatic Pressure ◽  
Human Tumor ◽  
Immunogenic Cell Death ◽  
Human Tumor Cells

High hydrostatic pressure to induce immunogenic cell death in human tumor cells.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. 3076-3076
Author(s):  
Iva Truxova ◽  
Jitka Fucikova ◽  
Irena Moserova ◽  
Simona Partlova ◽  
Jirina Bartunkova ◽  
...  
Keyword(s):  
T Cells ◽  
Cell Death ◽  
Hydrostatic Pressure ◽  
Tumor Cells ◽  
Cell Lines ◽  
Primary Tumor ◽  
High Hydrostatic Pressure ◽  
Human Tumor ◽  
Immunogenic Cell Death ◽  
Human Tumor Cells

3076 Background: Recent studies have identified molecular events characteristic of immunogenic cell death. These include surface exposure of calreticulin, HSP70 and HSP90, release of intranuclear HMGB1 and secretion of ATP from dying cells. Several chemotherapeutic agents, including anthracyclins, oxaliplatin and bortezomib, and hypericin-based photodynamic therapy have been described to induce the immunogenic cell death in human tumor cells. We investigated the potential of high hydrostatic pressure (HHP) to induce immunogenic cell death in human tumor cells. Methods: Prostate and ovarian cancer cell lines and primary tumor cells were treated by HHP and we analyzed the kinetics of the expression of immunogenic cell death markers. HHP killed tumor cells expressing immunogenic cell death markers were tested for their ability to activate dendritic cells (DCs), to induce tumor specific T cells and regulatory T cells. Results: HHP induced rapid expression of HSP70, HSP90 and calreticulin on the cell surface of all tested cell lines and primary tumor cells. HHP also induced release of HMGB1 and ATP from treated cells. The kinetics of expression was similar to doxorubicin, HHP, however, induced 1.5-2 fold higher expression of HSP70, HSP90 and calreticulin. The interaction of DCs with HHP-treated tumor cells led to the faster rate of phagocytosis, significant upregulation of CD83, CD86 and HLA-DR and release of IL-6, IL-12p70 and TNFα. The ability of HHP-killed tumor cells to promote DCs maturation was cell contact dependent. DCs pulsed with tumor cells killed by HHP induced high numbers of tumor-specific CD4+ and CD8+IFN-g-producing T cells even in the absence of additional maturation stimulus. DCs pulsed with HHP treated tumor cells also induced the lowest number of regulatory T cells among the tested conditions. Cells treated by HHP can by cryopreserved in liquid nitrogen and retain their immunogenic properties upon thawing thus allowing for their convenient use in the manufacturing of cancer immunotherapy products. Conclusions: High hydrostatic pressure is a reliable and very potent inducer of immunogenic cell death in the wide range of human tumor cell lines and primary tumor cells.

Induction of tumor cell death by high hydrostatic pressure as a novel supporting technique in orthopedic surgery

2003 ◽  
Author(s):  
Peter Diehl ◽  
Manfred Schmitt ◽  
Gerrit Blümelhuber ◽  
Benjamin Frey ◽  
Susanne Van Laak ◽  
...  
Keyword(s):  
Cell Death ◽  
Hydrostatic Pressure ◽  
Tumor Cell ◽  
Orthopedic Surgery ◽  
High Hydrostatic Pressure ◽  
Tumor Cell Death

Molecular Hydrogen Attenuates High Hydrostatic Pressure-Induced Neuronal Cell Damage by Reversing Dysfunction of Mitochondrial Electron Transfer Chain

2021 ◽  
Author(s):  
Zhuoyang Lu ◽  
Tiantian Zhang ◽  
Yachong Hu ◽  
Hui Liu ◽  
Li Cui ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Cell Damage ◽  
Protective Agent ◽  
Protective Effects ◽  
Human Neuroblastoma ◽  
Electron Transfer Chain ◽  
Pathological Conditions ◽  
Transfer Chain

Abstract Cellular hydrostatic pressure beyond its normal range can induce the accumulation of reactive oxidative species (ROS) generated by mitochondria and lead to pathological conditions such as glaucomatous optic neuropathy. However, little is known about how the mitochondrial electron transfer chain (ETC) is affected by elevated pressure. Moreover, the protective effects of hydrogen on various pathological conditions have been observed by reductions in ROS, yet the role of hydrogen in high hydrostatic pressure (HHP)-induced cell damage remains obscure. The goal of this study was to investigate the effect of HHP on ETC activity and whether hydrogen exerts protective effects against HHP-induced damage in cultured neuronal cells. Cultured SH-SY5Y human neuroblastoma cells were exposed to an elevated ambient hydrostatic pressure of 50 mmHg for a period of 2 to 6 h. HHP impaired the activities of ETC complexes, and these effects were reversed by hydrogen. Significant increases in apoptotic rates and intracellular ROS levels were observed in HHP-treated SH-SY5Y cells. Hydrogen significantly inhibited the apoptotic rates and reduced the levels of ROS. These findings suggest that HHP induces cell damage by causing ETC dysfunction to increase oxidative stress and that hydrogen may act as a protective agent to alleviate HHP-induced neuronal injury.

Antimicrobial effect of water‐soluble chitosans with high hydrostatic pressure

1991 ◽  
Vol 5 (1) ◽  
pp. 45-57 ◽  
Author(s):  
Anne M. Papineau ◽  
Dallas G. Hoover ◽  
Dietrich Knorr ◽  
Daniel F. Farkas
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Antimicrobial Effect ◽  
Water Soluble ◽  
Effect Of Water
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