scholarly journals Singlet Oxygen in Plants: Generation, Detection, and Signaling Roles

2020 ◽  
Vol 21 (9) ◽  
pp. 3237 ◽  
Author(s):  
Valeriya A. Dmitrieva ◽  
Elena V. Tyutereva ◽  
Olga V. Voitsekhovskaja
Keyword(s):  
Cell Death ◽  
Singlet Oxygen ◽  
Excited Electronic State ◽  
Plant Cells ◽  
Thylakoid Membranes ◽  
Reaction Centers ◽  
Biological Molecules ◽  
Photoinhibition Of Photosynthesis ◽  
Root Cells ◽  
High Concentrations

Singlet oxygen (1O2) refers to the lowest excited electronic state of molecular oxygen. It easily oxidizes biological molecules and, therefore, is cytotoxic. In plant cells, 1O2 is formed mostly in the light in thylakoid membranes by reaction centers of photosystem II. In high concentrations, 1O2 destroys membranes, proteins and DNA, inhibits protein synthesis in chloroplasts leading to photoinhibition of photosynthesis, and can result in cell death. However, 1O2 also acts as a signal relaying information from chloroplasts to the nucleus, regulating expression of nuclear genes. In spite of its extremely short lifetime, 1O2 can diffuse from the chloroplasts into the cytoplasm and the apoplast. As shown by recent studies, 1O2-activated signaling pathways depend not only on the levels but also on the sites of 1O2 production in chloroplasts, and can activate two types of responses, either acclimation to high light or programmed cell death. 1O2 can be produced in high amounts also in root cells during drought stress. This review summarizes recent advances in research on mechanisms and sites of 1O2 generation in plants, on 1O2-activated pathways of retrograde- and cellular signaling, and on the methods to study 1O2 production in plants.

scholarly journals Cold Atmospheric Pressure Plasma-Activated Medium Induces Selective Cell Death in Human Hepatocellular Carcinoma Cells Independently of Singlet Oxygen, Hydrogen Peroxide, Nitric Oxide and Nitrite/Nitrate

2021 ◽  
Vol 22 (11) ◽  
pp. 5548
Author(s):  
Yan Li ◽  
Tianyu Tang ◽  
Haejune Lee ◽  
Kiwon Song
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Singlet Oxygen ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Proliferative Effect ◽  
Huh7 Cells ◽  
Anti Cancer ◽  
Cold Atmospheric Pressure Plasma ◽  
Nitrite Nitrate

Cold atmospheric pressure plasma (CAP) and plasma-activated medium (PAM) induce cell death in diverse cancer cells and may function as powerful anti-cancer agents. The main components responsible for the selective anti-cancer effects of CAP and PAM remain elusive. CAP or PAM induces selective cell death in hepatocellular carcinoma cell lines Hep3B and Huh7 containing populations with cancer stem cell markers. Here, we investigated the major component(s) of CAP and PAM for mediating the selective anti-proliferative effect on Hep3B and Huh7 cells. The anti-proliferative effect of CAP was mediated through the medium; however, the reactive oxygen species scavenger N-acetyl cysteine did not suppress PAM-induced cell death. Neither high concentrations of nitrite or nitrite/nitrate nor a low concentration of H2O2 present in the PAM containing sodium pyruvate affected the viability of Hep3B and Huh7 cells. Inhibitors of singlet oxygen, superoxide anions, and nitric oxide retained the capacity of PAM to induce anti-cancer effects. The anti-cancer effect was largely blocked in the PAM prepared by placing an aluminum metal mesh, but not a dielectric PVC mesh, between the plasma source and the medium. Hence, singlet oxygen, hydrogen peroxide, nitric oxide, and nitrite/nitrate are not the main factors responsible for PAM-mediated selective death in Hep3B and Huh7 cells. Other factors, such as charged particles including various ions in CAP and PAM, may induce selective anti-cancer effects in certain cancer cells.

scholarly journals Singlet oxygen damages the function of Photosystem II in isolated thylakoids and in the green alga Chlorella sorokiniana

2021 ◽  
Author(s):  
Faiza Bashir ◽  
Ateeq Ur Rehman ◽  
Milán Szabó ◽  
Imre Vass
Keyword(s):  
Photosystem Ii ◽  
Singlet Oxygen ◽  
Screening Method ◽  
Physiological Role ◽  
Thylakoid Membranes ◽  
Chlorella Sorokiniana ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Intact Cells ◽  
Green Alga Chlorella

AbstractSinglet oxygen (1O2) is an important damaging agent, which is produced during illumination by the interaction of the triplet excited state pigment molecules with molecular oxygen. In cells of photosynthetic organisms 1O2 is formed primarily in chlorophyll containing complexes, and damages pigments, lipids, proteins and other cellular constituents in their environment. A useful approach to study the physiological role of 1O2 is the utilization of external photosensitizers. In the present study, we employed a multiwell plate-based screening method in combination with chlorophyll fluorescence imaging to characterize the effect of externally produced 1O2 on the photosynthetic activity of isolated thylakoid membranes and intact Chlorella sorokiniana cells. The results show that the external 1O2 produced by the photosensitization reactions of Rose Bengal damages Photosystem II both in isolated thylakoid membranes and in intact cells in a concentration dependent manner indicating that 1O2 plays a significant role in photodamage of Photosystem II.

Singlet oxygen feedback delayed fluorescence of protoporphyrin IX in organic solutions

2017 ◽  
Vol 16 (4) ◽  
pp. 507-518 ◽  
Author(s):  
Ivo S. Vinklárek ◽  
Marek Scholz ◽  
Roman Dědic ◽  
Jan Hála
Keyword(s):  
Partial Pressure ◽  
Singlet Oxygen ◽  
Protoporphyrin Ix ◽  
Clinical Applications ◽  
Delayed Fluorescence ◽  
Diagnostic Tools ◽  
Partial Pressure Of Oxygen ◽  
High Concentrations ◽  
Organic Solutions

The PpIX DF show the significant role of SOFDF mechanism at high concentrations and at atmospheric partial pressure of oxygen and should be considered when developing diagnostic tools for clinical applications.

PEGylated AIEgen Molecular Probe for Hypoxia-Mediated Tumor Imaging and Photodynamic Therapy

2021 ◽  
Author(s):  
Jun Li ◽  
Wei Liu ◽  
Zhuhao Li ◽  
Yingcai Hu ◽  
Jinfeng Yang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Singlet Oxygen ◽  
Tumor Imaging ◽  
Fluorescence Emission ◽  
Molecular Probe ◽  
Enhanced Fluorescence ◽  
High Concentrations

Unlike the aggregation caused quenching (ACQ) and reduced singlet oxygen (1O2) production of traditional photosensitizers at high concentrations, AIEgen photosensitizers show enhanced fluorescence emission and photosensitization ability in the aggregated...

Characterization of cell death process in plant cells induced by hipoxia and anoxia

2000 ◽  
Vol 28 (5) ◽  
pp. A372-A372
Author(s):  
E. N. Baranova ◽  
N. V. Kononenko ◽  
T. V. Bragina ◽  
G. M. Grineva ◽  
T. P. Astafurova ◽  
...  
Keyword(s):  
Cell Death ◽  
Plant Cells ◽  
Death Process ◽  
Cell Death Process

scholarly journals INFLUENCE OF LIGHT AND HEAVY LANTHANIDES ON THE PHYSIOLOGICAL PROCESSES OF Taraxacum hybernum

2020 ◽  
Vol 8 (Spl-2-AABAS) ◽  
pp. S298-S302
Author(s):  
Vladimir Nikolaevich Vorob’ev ◽  
◽  
Sergei Fedorovich Kotov ◽  
Vera Vladimirovna Nikolenko ◽  
Denis Vladimirovich Tishin ◽  
...  
Keyword(s):  
Quantum Efficiency ◽  
Photosynthetic Apparatus ◽  
Reaction Centers ◽  
Efficiency Change ◽  
Ps Ii ◽  
Antenna Complex ◽  
Physiological Processes ◽  
Light Lanthanide ◽  
Heavy Lanthanides ◽  
High Concentrations

The current study was carried out to study the influence of light and heavy lanthanides on the physiological process of Crimean-Sagyz/ Krim-saghyz (dandelion - Taraxacum hybernum). Lanthanide belongs to the group of light or heavy; infiltration of dandelion (Crimean saghyz) seeds with light and heavy lanthanides solutions increased the germination energy by 26%. The differences in the influence of light (cerium) and heavy (lutetium) were manifested in the quantum efficiency change of the photosystem 2 (PS II). Treatment of leaves with high concentrations (100 µM) led to a decrease of Y (II), moreover, under the influence of light lanthanide, the decrease was greater by 21%. It is assumed that the effect of the used lanthanides on the dandelion photosynthetic apparatus is multidirectional. Cerium influenced the PS II antenna complex, and lutetium influenced the reaction centers. A 10-fold decrease in the concentration did not change the nature of cerium action, except that Y (II) was restored already on the second day after treatment. The effect of lutetium became noticeable only by the 8th day after treatment when Y (II) became higher than that of untreated plants. Thus, the results of the study suggested that in dandelion leaves, lanthanides with a concentration of 10 µM increased the quantum efficiency of PS II in contrast to cerium.

Mechanisms of death induced by cisplatin in proximal tubular epithelial cells: apoptosis vs. necrosis

1996 ◽  
Vol 270 (4) ◽  
pp. F700-F708 ◽  
Author(s):  
W. Lieberthal ◽  
V. Triaca ◽  
J. Levine
Keyword(s):  
Cell Death ◽  
Dna Cleavage ◽  
Primary Cultures ◽  
Cell Monolayer ◽  
Membrane Integrity ◽  
Ladder Pattern ◽  
Proximal Tubular Epithelial Cells ◽  
Proximal Tubular ◽  
High Concentrations ◽  
Induced Apoptosis

We have examined the mechanisms of cell death induced by cisplatin in primary cultures of mouse proximal tubular cells. High concentrations of cisplatin (800 microM) led to necrotic cell death over a few hours. Much lower concentrations of cisplatin (8 microM) led to apoptosis, which caused loss of the cell monolayer over several days. Necrosis was characterized by a cytosolic swelling and early loss of plasma membrane integrity. In contrast, early features of cells undergoing apoptosis included cell shrinkage and loss of attachment to the monolayers. Nuclear chromatin became condensed and fragmented in apoptosing cells. These features were absent in necrotic cells. DNA electrophoresis of cells exposed to 800 microM cisplatin yielded a "smear" pattern, due to random DNA degradation. In contrast, the DNA of apoptosing cells demonstrated a "ladder" pattern resulting from internucleosomal DNA cleavage. Antioxidants delayed cisplatin-induced apoptosis but not necrosis. Thus the mechanism of cell death induced by cisplatin is concentration dependent. Reactive oxygen species play a role in mediating apoptosis but not necrosis induced by cisplatin.

Induction of the H+ Release from Thylakoid Membranes by Illumination in the Presence of Protonophores at High Concentrations

1991 ◽  
Vol 32 (7) ◽  
pp. 925-934 ◽  
Author(s):  
Hideo Yamasaki ◽  
Shigeki Furuya ◽  
Akira Kawamura ◽  
Akio Ito ◽  
Shigeki Okayama ◽  
...  
Keyword(s):  
Thylakoid Membranes ◽  
High Concentrations
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