scholarly journals Degradation, Bone Regeneration and Tissue Response of an Innovative Volume Stable Magnesium-Supported GBR/GTR Barrier Membrane

2020 ◽  
Vol 21 (9) ◽  
pp. 3098 ◽  
Author(s):  
Mike Barbeck ◽  
Lennart Kühnel ◽  
Frank Witte ◽  
Jens Pissarek ◽  
Clarissa Precht ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Mononuclear Cells ◽  
Fibrous Tissue ◽  
Tissue Response ◽  
Collagen Membrane ◽  
Sham Operation ◽  
Barrier Membrane ◽  
Synthetic Materials ◽  
Native Collagen

Introduction: Bioresorbable collagenous barrier membranes are used to prevent premature soft tissue ingrowth and to allow bone regeneration. For volume stable indications, only non-absorbable synthetic materials are available. This study investigates a new bioresorbable hydrofluoric acid (HF)-treated magnesium (Mg) mesh in a native collagen membrane for volume stable situations. Materials and Methods: HF-treated and untreated Mg were compared in direct and indirect cytocompatibility assays. In vivo, 18 New Zealand White Rabbits received each four 8 mm calvarial defects and were divided into four groups: (a) HF-treated Mg mesh/collagen membrane, (b) untreated Mg mesh/collagen membrane (c) collagen membrane and (d) sham operation. After 6, 12 and 18 weeks, Mg degradation and bone regeneration was measured using radiological and histological methods. Results: In vitro, HF-treated Mg showed higher cytocompatibility. Histopathologically, HF-Mg prevented gas cavities and was degraded by mononuclear cells via phagocytosis up to 12 weeks. Untreated Mg showed partially significant more gas cavities and a fibrous tissue reaction. Bone regeneration was not significantly different between all groups. Discussion and Conclusions: HF-Mg meshes embedded in native collagen membranes represent a volume stable and biocompatible alternative to the non-absorbable synthetic materials. HF-Mg shows less corrosion and is degraded by phagocytosis. However, the application of membranes did not result in higher bone regeneration.

scholarly journals In Vivo Analysis of the Biocompatibility and Macrophage Response of a Non-Resorbable PTFE Membrane for Guided Bone Regeneration

2018 ◽  
Vol 19 (10) ◽  
pp. 2952 ◽  
Author(s):  
Tadas Korzinskas ◽  
Ole Jung ◽  
Ralf Smeets ◽  
Sanja Stojanovic ◽  
Stevo Najman ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Foreign Body Response ◽  
Tissue Response ◽  
Collagen Membrane ◽  
Macrophage Response ◽  
Barrier Membrane ◽  
In Vivo Analysis ◽  
Inflammatory Macrophages

The use of non-resorbable polytetrafluoroethylene (PTFE) membranes is indicated for the treatment of large, non-self-containing bone defects, or multi-walled defects in the case of vertical augmentations. However, less is known about the molecular basis of the foreign body response to PTFE membranes. In the present study, the inflammatory tissue responses to a novel high-density PTFE (dPTFE) barrier membrane have preclinically been evaluated using the subcutaneous implantation model in BALB/c mice by means of histopathological and histomorphometrical analysis methods and immunohistochemical detection of M1- and M2-macrophages. A collagen membrane was used as the control material. The results of the present study demonstrate that the tissue response to the dPTFE membrane involves inflammatory macrophages, but comparable cell numbers were also detected in the implant beds of the control collagen membrane, which is known to be biocompatible. Although these data indicate that the analyzed dPTFE membrane is not fully bioinert, but its biocompatibility is comparable to collagen-based membranes. Based on its optimal biocompatibility, the novel dPTFE barrier membrane may optimally support bone healing within the context of guided bone regeneration (GBR).

scholarly journals Demineralized Dentine Material Membrane As Barrier Membrane for Bone Regeneration

2021 ◽  
Author(s):  
Pratiwi Soesilawati ◽  
Yuliati Yuliati ◽  
Agnes Paramitha Nastiti ◽  
Noor Hayaty Abu Kasim
Keyword(s):  
Treatment Group ◽  
Bone Graft ◽  
Bone Regeneration ◽  
Fibrous Tissue ◽  
Control Group ◽  
Collagen Membrane ◽  
P Value ◽  
Barrier Membrane ◽  
Collagen Density ◽  
Graft Implantation

Mandibular critical size defect (CSD) due to pathological conditions, trauma, and congenital disease can not heal spontaneously and predominantly filled with fibrous tissue. Therefore, a Guided Bone Regeneration (GBR) combined with bone grafting can be performed. The researchers considered using Demineralized Dentin Material Membrane (DDMM) from bovine dentine as an alternative GBR. This study aimed to determine the amount of fibroblast and collagen density after DDMM and bone graft implantation on CSD. Thirty-six Rattus norvegicus rats were used as samples. Mandibular bone defect 5x5 mm was made, then filled with bone graft and covered with Bovine Pericardium Collagen Membrane (BPCM) in the control group and DDMM in the treatment group. Six samples were sacrificed on 7, 14, and 21 days post-surgical for histology examination. There were no significant differences in the amount of fibroblast and collagen density (p-value > 0,05). The amount of fibroblast is lower and the collagen density is higher in treatment group. DDMM has microporosity to prevent connective tissue ingrowth and dentine tubules to allow growth factors release. DDMM and bone graft implantation can reduce the amount of fibroblasts and increase collagen density of CSD which potentially being used as a CSD alternative treatment for bone regeneration.

scholarly journals Dentin-Derived-Barrier Membrane in Guided Bone Regeneration: A Case Report

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2166
Author(s):  
Jeong-Kui Ku ◽  
In-Woong Um ◽  
Mi-Kyoung Jun ◽  
Il-hyung Kim
Keyword(s):  
Case Report ◽  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Successful Outcome ◽  
Collagen Membrane ◽  
Type I ◽  
Barrier Membrane ◽  
Dentin Matrix ◽  
Demineralized Dentin Matrix ◽  
Demineralized Dentin

An autogenous, demineralized, dentin matrix is a well-known osteo-inductive bone substitute that is mostly composed of type I collagen and is widely used in implant dentistry. This single case report describes a successful outcome in guided bone regeneration and dental implantation with a novel human-derived collagen membrane. The authors fabricated a dentin-derived-barrier membrane from a block-type autogenous demineralized dentin matrix to overcome the mechanical instability of the collagen membrane. The dentin-derived-barrier acted as an osteo-inductive collagen membrane with mechanical and clot stabilities, and it replaced the osteo-genetic function of the periosteum. Further research involving large numbers of patients should be conducted to evaluate bone forming capacity in comparison with other collagen membranes.

Superficial Topography and Porosity of an Absorbable Barrier Membrane Impacts Soft Tissue Response in Guided Bone Regeneration

2010 ◽  
Vol 81 (6) ◽  
pp. 926-933 ◽  
Author(s):  
Ronaldo Barcellos de Santana ◽  
Carolina Miller Leite de Mattos ◽  
Carlos Eduardo Francischone ◽  
Thomas Van Dyke
Keyword(s):  
Soft Tissue ◽  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Tissue Response ◽  
Barrier Membrane ◽  
Soft Tissue Response

scholarly journals A Growth Factor-Free Co-Culture System of Osteoblasts and Peripheral Blood Mononuclear Cells for the Evaluation of the Osteogenesis Potential of Melt-Electrowritten Polycaprolactone Scaffolds

2019 ◽  
Vol 20 (5) ◽  
pp. 1068 ◽  
Author(s):  
Andreas Hammerl ◽  
Carlos Diaz Cano ◽  
Elena De-Juan-Pardo ◽  
Martijn van Griensven ◽  
Patrina Poh
Keyword(s):  
Growth Factor ◽  
Bone Regeneration ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Culture System ◽  
Mononuclear Cells ◽  
Regeneration Potential ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Scaffolds made of biodegradable biomaterials are widely used to guide bone regeneration. Commonly, in vitro assessment of scaffolds’ osteogenesis potential has been performed predominantly in monoculture settings. Hence, this study evaluated the potential of an unstimulated, growth factor-free co-culture system comprised of osteoblasts (OB) and peripheral blood mononuclear cells (PBMC) over monoculture of OB as an in vitro platform for screening of bone regeneration potential of scaffolds. Particularly, this study focuses on the osteogenic differentiation and mineralized matrix formation aspects of cells. The study was performed using scaffolds fabricated by means of a melt electrowriting (MEW) technique made of medical-grade polycaprolactone (PCL), with or without a surface coating of calcium phosphate (CaP). Qualitative results, i.e., cell morphology by fluorescence imaging and matrix mineralization by von Kossa staining, indicated the differences in cell behaviours in response to scaffolds’ biomaterial. However, no obvious differences were noted between OB and OB+PBMC groups. Hence, quantitative investigation, i.e., alkaline phosphatase (ALP), tartrate-resistant acid phosphatase (TRAP) activities, and gene expression were quantitatively evaluated by reverse transcription-polymerase chain reaction (RT-qPCR), were evaluated only of PCL/CaP scaffolds cultured with OB+PBMC, while PCL/CaP scaffolds cultured with OB or PBMC acted as a control. Although this study showed no differences in terms of osteogenic differentiation and ECM mineralization, preliminary qualitative results indicate an obvious difference in the cell/non-mineralized ECM density between scaffolds cultured with OB or OB+PBMC that could be worth further investigation. Collectively, the unstimulated, growth factor-free co-culture (OB+PBMC) system presented in this study could be beneficial for the pre-screening of scaffolds’ in vitro bone regeneration potential prior to validation in vivo.

scholarly journals The Condensation of Collagen Leads to an Extended Standing Time and a Decreased Pro-inflammatory Tissue Response to a Newly Developed Pericardium-based Barrier Membrane for Guided Bone Regeneration

In Vivo ◽  
2020 ◽  
Vol 34 (3) ◽  
pp. 985-1000 ◽  
Author(s):  
TRISTAN GUELDENPFENNIG ◽  
ALIREZA HOUSHMAND ◽  
STEVO NAJMAN ◽  
SANJA STOJANOVIC ◽  
TADAS KORZINSKAS ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Tissue Response ◽  
Standing Time ◽  
Barrier Membrane ◽  
Inflammatory Tissue

scholarly journals Biocompatibility and Immune Response of a Newly Developed Volume-Stable Magnesium-Based Barrier Membrane in Combination with a PVD Coating for Guided Bone Regeneration (GBR)

Biomedicines ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 636
Author(s):  
Larissa Steigmann ◽  
Ole Jung ◽  
Wolfgang Kieferle ◽  
Sanja Stojanovic ◽  
Annica Proehl ◽  
...  
Keyword(s):  
Immune Response ◽  
Collagen Membrane ◽  
Promising Alternative ◽  
Barrier Membrane ◽  
Pvd Coating ◽  
Coated Membranes ◽  
Gas Cavity ◽  
Inflammatory Macrophages

To date, there are no bioresorbable alternatives to non-resorbable and volume-stable membranes in the field of dentistry for guided bone or tissue regeneration (GBR/GTR). Even magnesium (Mg) has been shown to constitute a favorable biomaterial for the development of stabilizing structures. However, it has been described that it is necessary to prevent premature degradation to ensure both the functionality and the biocompatibility of such Mg implants. Different coating strategies have already been developed, but most of them did not provide the desired functionality. The present study analyses a new approach based on ion implantation (II) with PVD coating for the passivation of a newly developed Mg membrane for GBR/GTR procedures. To demonstrate comprehensive biocompatibility and successful passivation of the Mg membranes, untreated Mg (MG) and coated Mg (MG-Co) were investigated in vitro and in vivo. Thereby a collagen membrane with an already shown biocompatibility was used as control material. All investigations were performed according to EN ISO 10993 regulations. The in vitro results showed that both the untreated and PVD-coated membranes were not cytocompatible. However, both membrane types fulfilled the requirements for in vivo biocompatibility. Interestingly, the PVD coating did not have an influence on the gas cavity formation compared to the uncoated membrane, but it induced lower numbers of anti-inflammatory macrophages in comparison to the pure Mg membrane and the collagen membrane. In contrast, the pure Mg membrane provoked an immune response that was fully comparable to the collagen membrane. Altogether, this study shows that pure magnesium membranes represent a promising alternative compared to the nonresorbable volume-stable materials for GBR/GTR therapy.

scholarly journals Effect of Multilaminate Small Intestinal Submucosa as a Barrier Membrane on Bone Formation in a Rabbit Mandible Defect Model

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Weiyi Wu ◽  
Bowen Li ◽  
Yuhua Liu ◽  
Xinzhi Wang ◽  
Lin Tang
Keyword(s):  
Bone Regeneration ◽  
Porous Scaffold ◽  
Small Intestinal Submucosa ◽  
Control Group ◽  
Collagen Membrane ◽  
Defect Model ◽  
Barrier Membrane ◽  
Small Intestinal ◽  
Intestinal Submucosa

A barrier membrane (BM) is essential for guided bone regeneration (GBR) procedures. Absorbable BMs based on collagen have been widely applied clinically due to their excellent biocompatibility. The extracellular matrix (ECM) provides certain advantages that can compensate for the rapid degradation and insufficient mechanical strength of pure collagen membrane due to the porous scaffold structure. Recently, small intestinal submucosa (SIS), one of the most widely used ECM materials, has drawn much attention in bone tissue engineering. In this study, we adopted multilaminate SIS (mSIS) as a BM and evaluated its in vivo and in vitro properties. mSIS exhibited a multilaminate structure with a smooth upper surface and a significantly coarser bottom layer according to microscopic observation. Tensile strength was 13.10 ± 2.56 MPa. In in vivo experiments, we selected a rabbit mandibular defect model and subcutaneous implantation to compare osteogenesis and biodegradation properties with one of the most commonly used commercial collagen membranes. mSIS was retained for up to 3 months and demonstrated longer biodegradation time than commercial collagen membrane. Quantification of bone regeneration revealed significant differences in each group. Micro-computed tomography (micro-CT) revealed that the quantity and maturity of bones in the mSIS group were significantly higher than those in the blank control group (P < 0.05) and were similar to those in a commercial collagen membrane group (P > 0.05) at 4 and 12 weeks after surgery. Hematoxylin and eosin staining revealed large amounts of mature lamellar bone at 12 weeks in mSIS and commercial collagen membrane groups. Therefore, we conclude that mSIS has potential as a future biocompatible BM in GBR procedures.

scholarly journals In Vitro and In Vivo Study of a Novel Porcine Collagen Membrane for Guided Bone Regeneration

Materials ◽  
2016 ◽  
Vol 9 (11) ◽  
pp. 949 ◽  
Author(s):  
Eisner Salamanca ◽  
Chi-Yang Tsai ◽  
Yu-Hwa Pan ◽  
Yu-Te Lin ◽  
Haw-Ming Huang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
In Vivo Study ◽  
Collagen Membrane ◽  
Porcine Collagen

scholarly journals Application of EGCG modified EDC/NHS cross-linked extracellular matrix to promote macrophage adhesion

2020 ◽  
Author(s):  
Chenyu Chu ◽  
Shengan Rung ◽  
Renli Yang ◽  
Yi Man ◽  
Yili Qu
Keyword(s):  
Mechanical Properties ◽  
Surface Morphology ◽  
Bone Regeneration ◽  
Foreign Body Reaction ◽  
Collagen Membrane ◽  
Regulatory Effect ◽  
Subcutaneous Implantation ◽  
Implant Dentistry ◽  
Macrophage Adhesion

AbstractThough chemically cross-linked by EDC/NHS endows collagen membrane with promising mechanical properties, it is not conducive to modulation of foreign body reaction (FBR) after implantation or guidance of osteogenesis. In our previous research, we have found that macrophages have a strong regulatory effect on tissue and bone regeneration during FBR, and EGCG modified membranes could adjust the recruitment and phenotypes of macrophages. Accordingly, we develop the EGCG-EDC/NHS membranes, prepared with physically immersion, while the surface morphology of the membrane was observed by SEM, the biological activity of collagen was determined by FTIR, the activity and adhesion of cell culture in vitro, angiogenesis and monocyte/macrophage recruitment after subcutaneous implantation, etc. are characterized. It could be concluded that EGCG-EDC/NHS collagen membrane is hopeful to be used in implant dentistry for it not only retains the advantages of the collagen membrane itself, but also improves cell viability, adhesion and vascularization tendency. However, the mechanism that lies in the regenerative advantages of such membrane needs further exploration, but it is certain that the differences in surface morphology can have a significant impact on the reaction between the host and the implant, not to mention macrophage in bone regeneration.

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