scholarly journals Relaxation Dynamics of Chlorophyll b in the Sub-ps Ultrafast Timescale Measured by 2D Electronic Spectroscopy

2020 ◽  
Vol 21 (8) ◽  
pp. 2836
Author(s):  
Elisa Fresch ◽  
Elisabetta Collini
Keyword(s):  
Internal Conversion ◽  
Electronic Spectroscopy ◽  
Early Time ◽  
Relaxation Processes ◽  
Ultrafast Dynamics ◽  
Chlorophyll B ◽  
Relaxation Dynamics ◽  
Biologically Relevant ◽  
Harvesting Systems

A thorough characterization of the early time sub-100 fs relaxation dynamics of biologically relevant chromophores is of crucial importance for a complete understanding of the mechanisms regulating the ultrafast dynamics of the relaxation processes in more complex multichromophoric light-harvesting systems. While chlorophyll a has already been the object of several investigations, little has been reported on chlorophyll b, despite its pivotal role in many functionalities of photosynthetic proteins. Here the relaxation dynamics of chlorophyll b in the ultrafast regime have been characterized using 2D electronic spectroscopy. The comparison of experimental measurements performed at room temperature and 77 K allows the mechanisms and the dynamics of the sub-100 fs relaxation dynamics to be characterized, including spectral diffusion and fast internal conversion assisted by a specific set of vibrational modes.

Coordination Compounds of Some 3d-Transition Metal Ions with N1-[4-(4-bromo-phenylsulfonyl)-benzoyl]-N4-(4-methoxyphenyl)- thiosemicarbazide

2008 ◽  
Vol 59 (7) ◽  
Author(s):  
Madalina Angelusiu ◽  
Maria Negoiu ◽  
Stefania-Felicia Barbuceanu ◽  
Tudor Rosu
Keyword(s):  
Coordination Compounds ◽  
Magnetic Moments ◽  
Thermo Gravimetric Analysis ◽  
Electronic Spectroscopy ◽  
Transition Metal Ions ◽  
Synthesis And Characterization ◽  
Gravimetric Analysis ◽  
Thermo Gravimetric ◽  
New Compounds

The paper presents the synthesis and characterization of Cu(II), Co(II), Ni(II), Cd(II), Zn(II) and Hg(II) complexes with N1-[4-(4-bromo-phenylsulfonyl)-benzoyl]-N4-(4-methoxyphenyl)-thiosemicarbazide. The new compounds were characterized by IR, EPR, electronic spectroscopy, magnetic moments, thermo-gravimetric analysis and elemental analysis.

Relaxation Dynamics and Structural Characterization of Organic Nanoparticles with Enhanced Emission

2005 ◽  
Vol 109 (28) ◽  
pp. 13472-13482 ◽  
Author(s):  
Chetan Jagdish Bhongale ◽  
Chih-Wei Chang ◽  
Chi-Shen Lee ◽  
Eric Wei-Guang Diau ◽  
Chain-Shu Hsu
Keyword(s):  
Structural Characterization ◽  
Relaxation Dynamics ◽  
Organic Nanoparticles

Th(iv) and Ce(iv) napthylsalophen sandwich complexes: characterization of unusual thorium fluorescence in solution and solid-state

2017 ◽  
Vol 53 (88) ◽  
pp. 11984-11987 ◽  
Author(s):  
E. E. Hardy ◽  
K. M. Wyss ◽  
J. D. Gorden ◽  
I. R. Ariyarathna ◽  
E. Miliordos ◽  
...  
Keyword(s):  
Solid State ◽  
Electronic Spectroscopy ◽  
Sandwich Complexes

The synthesis, characterization, and surprising electronic spectroscopy of two ML2 sandwich complexes, where M = Ce(iv) or Th(iv) and L = napthylsalophen2− are described.

scholarly journals β-Lactamase Tools for Establishing Cell Internalization and Cytosolic Delivery of Cell Penetrating Peptides

Biomolecules ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 51 ◽  
Author(s):  
Shane Stone ◽  
Tatjana Heinrich ◽  
Suzy Juraja ◽  
Jiulia Satiaputra ◽  
Clinton Hall ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Cell Penetrating Peptides ◽  
Stable Cell Line ◽  
Intracellular Space ◽  
Biologically Relevant ◽  
Cell Internalization ◽  
Cytosolic Delivery ◽  
Cell Penetrating ◽  
Split Protein

The ability of cell penetrating peptides (CPPs) to deliver biologically relevant cargos into cells is becoming more important as targets in the intracellular space continue to be explored. We have developed two assays based on CPP-dependent, intracellular delivery of TEM-1 β-lactamase enzyme, a functional biological molecule comparable in size to many protein therapeutics. The first assay focuses on the delivery of full-length β-lactamase to evaluate the internalization potential of a CPP sequence. The second assay uses a split-protein system where one component of β-lactamase is constitutively expressed in the cytoplasm of a stable cell line and the other component is delivered by a CPP. The delivery of a split β-lactamase component evaluates the cytosolic delivery capacity of a CPP. We demonstrate that these assays are rapid, flexible and have potential for use with any cell type and CPP sequence. Both assays are validated using canonical and novel CPPs, with limits of detection from <500 nM to 1 µM. Together, the β-lactamase assays provide compatible tools for functional characterization of CPP activity and the delivery of biological cargos into cells.

scholarly journals Applications of neutron reflectometry in biology

2020 ◽  
Vol 236 ◽  
pp. 04002 ◽  
Author(s):  
Yuri Gerelli
Keyword(s):  
Thin Films ◽  
Structural Features ◽  
Neutron Reflectometry ◽  
Protein Films ◽  
Biologically Relevant ◽  
Buried Interfaces ◽  
Biological Thin Films ◽  
Non Destructive

Over the last 10 years, neutron reflectometry (NR) has emerged as a powerful technique for the investigation of biologically relevant thin films. The great advantage of NR with respect to many other surface-sensitive techniques is its sub-nanometer resolution that enables structural characterizations at the molecular level. In the case of bio-relevant samples, NR is non-destructive and can be used to probe thin films at buried interfaces or enclosed in bulky sample environment equipment. Moreover, recent advances in biomolecular deutera-tion enabled new labeling strategies to highlight certain structural features and to resolve with better accuracy the location of chemically similar molecules within a thin film. In this chapter I will describe some applications of NR to bio-relevant samples and discuss some of the data analysis approaches available for biological thin films. In particular, examples on the structural characterization of biomembranes, protein films and protein-lipid interactions will be described.

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