scholarly journals HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution

2020 ◽  
Vol 21 (8) ◽  
pp. 2667 ◽  
Author(s):  
Katarzyna Sitarz ◽  
Krzysztof Czamara ◽  
Joanna Bialecka ◽  
Malgorzata Klimek ◽  
Barbara Zawilinska ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Glycogen Metabolism ◽  
Hpv Infection ◽  
Raman Microscopy ◽  
Glycogen Depletion ◽  
Large Nucleus ◽  
Phenotypic Differences ◽  
Cancer Group ◽  
Cervical Cells ◽  
Subcellular Resolution

Using Raman microscopy, we investigated epithelial cervical cells collected from 96 women with squamous cell carcinoma (SCC) or belonging to groups I, IIa, IIID-1 and IIID-2 according to Munich III classification (IIID-1 and IIID-2 corresponding to Bethesda LSIL and HSIL groups, respectively). All women were tested for human papillomavirus (HPV) infection using PCR. Subcellular resolution of Raman microscopy enabled to understand phenotypic differences in a heterogeneous population of cervical cells in the following groups: I/HPV−, IIa/HPV−, IIa/HPV−, LSIL/HPV−, LSIL/HPV+, HSIL/HPV−, HSIL/HPV+ and cancer cells (SCC/HPV+). We showed for the first time that the glycogen content in the cytoplasm decreased with the nucleus size of cervical cells in all studied groups apart from the cancer group. For the subpopulation of large-nucleus cells HPV infection resulted in considerable glycogen depletion compared to HPV negative cells in IIa, LSIL (for both statistical significance, ca. 45%) and HSIL (trend, 37%) groups. We hypothesize that accelerated glycogenolysis in large-nucleus cells may be associated with the increased protein metabolism for HPV positive cells. Our work underlines unique capabilities of Raman microscopy in single cell studies and demonstrate potential of Raman-based methods in HPV diagnostics.

scholarly journals Human papillomavirus and human telomerase RNA component gene in cervical cancer progression

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Yang Liu ◽  
Pianping Fan ◽  
Yingying Yang ◽  
Changjun Xu ◽  
Yajuan Huang ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Cancer Progression ◽  
Invasive Cervical Cancer ◽  
Intraepithelial Neoplasia ◽  
Hpv Infection ◽  
Cancer Group ◽  
Potential Marker ◽  
Significant Difference ◽  
Human Telomerase

Abstract This study aimed to examine hTERC gene in different grades of cervical intraepithelial neoplasia (CIN) and cervical cancer, and the association between hTERC and high risk-human papillomavirus (HR-HPV) infection. Patients who underwent cervical cancer screening at the Second Affiliated Hospital of Kunming Medical University between October 2010 and December 2011 were enrolled. All patients underwent liquid-based cytology test and hybrid capture 2 (HC2) for HPV detection. hTERC was examined using fluorescence in situ hybridization (FISH). Cervical colposcopy biopsy was performed if any of the three results was positive. HC2, FISH, and pathology were compared. A total of 1200 women underwent screening, 150 patients underwent cervical biopsy: 32 in the normal group, 38 in the CIN1 group, 66 in the CIN2/3 group, and 14 in the invasive cervical cancer group. More patients had HR-HPV infection in the CIN2/3 group and ICC group compared with the CIN1 group. hTERC increased with increasing histological dysplasia. There was significant difference in hTERC positive rate between each of the three groups. More patients with hTERC gene amplification were observed in the positive HR-HPV group than in the HR-HPV negative group. In conclusion, hTERC is a potential marker for precancerous cervical cancer lesions. hTERC might be correlated with HR-HPV infection in cervical diseases.

scholarly journals Prognostic value of p16INK4a overexpression in penile cancer

2020 ◽  
Vol 92 (1) ◽  
pp. 11-16
Author(s):  
Mário Pereira-Lourenço ◽  
Duarte Vieira e Brito ◽  
Miguel Eliseu ◽  
Noémia Castelo-Branco ◽  
João Pedro Peralta ◽  
...  
Keyword(s):  
Penile Cancer ◽  
Statistical Significance ◽  
Disease Free Survival ◽  
Hpv Infection ◽  
Industrialized Countries ◽  
Relevant Factor ◽  
Nodal Staging ◽  
Cancer Specific Survival ◽  
Carcinogenic Effect ◽  
The One

Introduction: Penile cancer is rare, accounting for less than 1% of all male cancers in industrialized countries. It is most common in areas of high prevalence of HPV, being a third of cases attributed to the carcinogenic effect of HPV. Tumour cells infected with HPV overexpress p16INK4a, as such p16INK4a has been used as a surrogate of HPV infections. Objective: To evaluate the prognostic factor of p16INK4a overexpression in penile cancer. Methods: Retrospective analysis of patients diagnosed with penile cancer, submitted to surgery in a Portuguese Oncological Institution in the last 20 years (n = 35). Histological review of surgical pieces and immunohistochemical identification of p16INK4a. Relation between p16INK4a and the following factors were studied: age, histological subtype, tumour dimensions, grade, TNM stage, perineural invasion, perivascular invasion, disease free survival (DFS) and cancer specific survival (CSS). Results: p16INK4a was positive in 8 patients (22.9%). Identification of p16INK4a did not correlate with none of the histopathological factors. In this work we identified a better DFS and CSS in patients positive for p16INK4a (DFS at 36 months was 100.0% vs. 66.7%; CSS at 36 months was 100.0% vs. 70.4%), although without statistical significance (p > 0.05). In multivariate analysis of histopathological factors studied, only N staging correlated with DFS and CSS (p = 0.017 and p = 0.014, respectively). Discussion: the percentage of cases positive for p16INK4a is smaller than the one found in literature, which can suggest a less relevant part of HPV infection in the oncogenesis of penile cancer in the studied population. Identification of p16INK4a did not relate with other clinicopathological factors. Tendency for a more favourable prognosis in patients with p16INK4a agrees with results found in literature. The most relevant factor for prognosis is nodal staging. Conclusions: penile cancer positive for p16INK4a shows a trend for better survival, although the most relevant factor is nodal staging.

Adrenal hormones enhance glycogenolysis in nonexercising muscle during exercise

1987 ◽  
Vol 63 (3) ◽  
pp. 1275-1283 ◽  
Author(s):  
J. C. McDermott ◽  
G. C. Elder ◽  
A. Bonen
Keyword(s):  
Skeletal Muscle ◽  
Contractile Activity ◽  
Glycogen Metabolism ◽  
Plasma Epinephrine ◽  
Glycogen Depletion ◽  
Adrenal Hormones ◽  
Epinephrine Injection ◽  
Hindlimb Muscles ◽  
Exercise Period ◽  
White Gastrocnemius

The purpose of this study was to investigate whether epinephrine exerts an effect on glycogen metabolism in nonexercising (Non-Ex) as well as in exercising (Ex) skeletal muscle. Rats ran (15 m/min; 8% grade) on their forelimbs while their hindlimbs (Non-Ex) were suspended above the treadmill. Electromyographic records confirmed the lack of significant contractile activity in muscles during suspension. Plasma epinephrine levels were manipulated in three experimental groups (n = 20 for each group): adrenalectomized (ADX), intact adrenals (IA), and IA + epinephrine injection (+Ep). Another group of rats performed normal exercise on all four limbs (15 m/min; 8% grade). Muscle glycogen levels were measured in selected hindlimb muscles at t = 0 and after 90 min exercise (15 m/min; 8% grade) or suspended rest. In the absence of epinephrine (ADX), no glycogen loss was found (P greater than 0.05) in Non-Ex muscles during the exercise period. In the IA group (epinephrine levels elevated sixfold above basal at t = 90 min), glycogen levels in the nonexercising soleus, plantaris, and red and white gastrocnemius were significantly (P less than 0.05) depleted to 62 +/- 6, 67 +/- 6, 58 +/- 5, and 67 +/- 9% of control values, respectively. Similar decrements occurred in these muscles when exercise was performed on all four limbs (P greater than 0.05). We conclude that glycogenolysis occurs in nonexercising skeletal muscle independent of contractile activity, probably due to the effect of epinephrine. Furthermore, the present data strongly suggest that glycogen depletion patterns in muscles during exercise cannot be used as an index of motor unit recruitment.

Higher levels of oxidative DNA damage in cervical cells are correlated with the grade of dysplasia and HPV infection

2015 ◽  
Vol 88 (2) ◽  
pp. 336-344 ◽  
Author(s):  
Giuseppa Visalli ◽  
Romana Riso ◽  
Alessio Facciolà ◽  
Placido Mondello ◽  
Carmela Caruso ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Hpv Infection ◽  
Cervical Cells

scholarly journals Influence of a single-nucleotide polymorphism of AKT1 (rs2498796) and HEY2 (rs13328928) genes on the risk of endometrial cancer in women of the Republic of Tatarstan

2019 ◽  
Vol 100 (5) ◽  
pp. 769-773
Author(s):  
R I Gabidullina ◽  
F R Nukhbala ◽  
G A Smirnova ◽  
Yu I Orlova ◽  
A A Shakirov ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Endometrial Cancer ◽  
Statistical Significance ◽  
Control Group ◽  
Endometrioid Carcinoma ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Cancer Group ◽  
The Republic ◽  
Group 2

Aim. To analyze the prevalence of different polymorphisms of AKT1 gene (rs2498796) and HEY2 gene (rs13328928) and to determine the association of revealed polymorphisms with the risk of endometrioid carcinoma in women living in the Republic of Tatarstan. Methods. 161 female citizens of Tatarstan were enrolled. The study group included 60 patients with endometrial cancer (endometrioid carcinoma) and the control group enrolled 101 women without endometrial pathology. The age of the subjects ranged from 41 to 91 years. The single-nucleotide polymorphism of AKT1 gene (rs2498796) and HEY2 gene (rs13328928) was determined by real-time polymerase chain reaction. We ran a 2 test and evaluated the odds ratio. Results. The risk of endometrial cancer was higher in carriers of homozygous T/T genotype of AKT1 gene (rs2498796) without statistical significance (OR=1.61, 95% CI=0.614.21, p=0.62). Homozygous C/C genotype of HEY2 gene (rs13328928) with the mutant allele C was observed in endometrial cancer group with a frequency of 0.383 and 0.287 in the control group (2=1.70, p=0.43). The risk of endometrial cancer was higher in the group of homozygous C/C genotype without statistical significance (OR=1.54, 95% CI=0.793.03, p=0.43). Conclusion. Among 161 females citizens of the Republic of Tatarstan included into the study, the associations of the mutant alleles of AKT1 gene (rs2498796) and HEY2 gene (rs13328928) with the risk of endometrial cancer were not identified; the prevalence of alleles and genotypes was found to be comparable with the European one.

scholarly journals Th1 Cytokine Patterns in Cervical Human Papillomavirus Infection

1999 ◽  
Vol 6 (5) ◽  
pp. 751-755 ◽  
Author(s):  
Mark Scott ◽  
Daniel P. Stites ◽  
Anna-Barbara Moscicki
Keyword(s):  
Human Papillomavirus ◽  
Hpv Infection ◽  
Interleukin 4 ◽  
Cell Mediated Immunity ◽  
Cross Sectional ◽  
Hpv Dna ◽  
Reverse Transcription Pcr ◽  
Th1 Cytokine ◽  
Cervical Cells ◽  
Cervical Human Papillomavirus

ABSTRACT The host’s immune response to cervical human papillomavirus (HPV) infection is poorly understood. In a longitudinal cohort of women with cervical HPV infections, defined by PCR-based HPV DNA testing, we used exfoliated cervical cells and reverse transcription-PCR to examine the cervical mucosal mRNA expression of cytokines involved in regulating cell-mediated immunity. We identified seven HPV-positive subjects who were found to have cleared their HPV infections 4 months later. In all seven, a T-helper type 1 (Th1) cytokine pattern (expression of gamma interferon and absence of interleukin-4) preceded clearance. The more variable cytokine patterns seen in HPV-negative subjects suggest that the Th1 pattern in the women with subsequent clearance was a response to the HPV infection. This contention is supported by additional cross-sectional data showing a Th1 pattern in a majority of HPV-positive women. This study establishes a feasible means for assessing local cytokine expression in the cervical milieu and demonstrates that a Th1 cytokine response is associated with subsequent clearance of cervical HPV infection.

scholarly journals Research Progress of HPV L1 Capsid Protein in Prediction of Cervical Lesions

2020 ◽  
Vol 4 (6) ◽  
Author(s):  
Jing Cheng ◽  
Lin Xu ◽  
Beibei Liu ◽  
Biao Wang ◽  
Xicui Long ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Capsid Protein ◽  
Hpv Infection ◽  
Research Progress ◽  
Patient Treatment ◽  
Structural Protein ◽  
Cervical Lesions ◽  
Cervical Cells ◽  
Cellular Immune

Cervical cancer is one of the most common malignant gynecological tumors and has the second highest incidence of all malignancies in females. Chronic and persistent infection with High Risk Human Papillomavirus (HR-HPV) is the main cause of cervical cancer. There is a distinct lack of methodology by which to determine whether cervical epithelial dysplasia is cancerous following HPV infection. HPV L1 capsid protein is a major structural protein of human papillomavirus (HPV)?and it is the main target of the local cellular immune response aiming to combat human papillomavirus after HPV infection within cervical cells. Greater understanding of HPV L1 capsid protein and its association with cervical cytology, histopathology, patient age and human papillomavirus viral load has the potential to contribute toward improved the improved diagnosis and management of cervical cancer, providing useful information for gynecological clinicians in the hope of improving patient treatment and quality of life. This article reviews the predictive utility of HPV L1 capsid protein for cervical lesions.

scholarly journals Genome of Helicobacter pylori and Serotype of HPV Detected in Oropharyngeal and Laryngeal Cancer and Chronic Inflammation Patients

2021 ◽  
Vol 18 (18) ◽  
pp. 9545
Author(s):  
Jaromír Astl ◽  
Richard Holy ◽  
Eva Maute ◽  
Jan Rotnágl ◽  
David Kalfeřt ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
High Risk ◽  
Cancer Patients ◽  
Chronic Inflammation ◽  
Laryngeal Cancer ◽  
Hpv Infection ◽  
Unknown Primary ◽  
Control Group ◽  
Palatine Tonsils ◽  
Cancer Group

Objective: Oropharyngeal/laryngeal carcinoma are common cancers of the upper aerodigestive system. Human papillomavirus (HPV) is described as the most frequent in the cancer of unknown primary. The presence of Helicobacter pylori (HP) in the oral cavity is discussed in some papers. The aim of study: To analyze the incidence of HPV and HP in oropharyngeal/laryngeal cancer persons versus persons with chronic tonsillar inflammation and healthy persons. Methods: The samples were taken in three groups: (1) tissue of oropharynx/larynx cancer (103 specimens); (2) tissue of palatine tonsils (85 specimens); and (3) healthy control group (50 specimens). We analyzed the presence of HP (PCR) and HPV genomic DNA (Sacace HPV High-Risk Screen Real-TM Quant) in the samples. Results: HP was detected in 86 samples (83.5%) and high-risk HPV in 62 samples (60.2%). We found a very high incidence of HP. In the cancer group, HP was detected in 82.5% cases and HPV positivity in 57.8%. In total, 7.2% of the cancer patients were negative for HP and HPV together. In turn, 53.6% of the cancer patients were positive for HP and HPV together. Four cases (4.2%) were positive for HPV only. VacA positivity was detected in 82 (79.6%) of the cancer cases and VacA negativity in 21 (20.4%) if the cancer cases. The incidence of HP in chronic inflammation (n = 85) was 65 cases (76.5%) and the incidence of HPV was 38 cases (44.7%). VacA positivity was detected in 59 (69.4%) of the chronic inflammation cases and VacA negativity was found in 26 (30.6%) of the chronic inflammation cases. Regarding the control group, we found HP positivity in 5 cases (11.1%) and HPV positivity in 19 cases (42.2%). There was VacA positivity in 6 cases (50.0%) of the control group. Statistically significantly lower prevalence of HP (p < 0.001) and HPV (p = 0.006) was found in the control group. Conclusions: We suggest that the palatine tonsils are colonized by HP. In our study, HP was present in oropharyngeal cancer in more cases in comparison with HPV infection. The presence of VacA from HP can have an influence on the human epithelial and immune cells’ regulation ways. Our results do not support idea that the CagA-positive HP is a primary carcinogen in oropharyngeal area.

scholarly journals Cervical Cancer Screening in Nepal

2013 ◽  
Vol 12 (1) ◽  
pp. 18-20 ◽  
Author(s):  
M Joshi ◽  
SR Mishra
Keyword(s):  
Cervical Cancer ◽  
Cancer Screening ◽  
Cervical Cancer Screening ◽  
Pap Smear ◽  
Screening Program ◽  
Hpv Infection ◽  
Cancer Screening Program ◽  
Special Programs ◽  
Cervical Cancer Screening Program ◽  
Cervical Cells

Cervical cancer screening is a process using the Pap smear to identify precancerous and potentially precancerous changes in cervical cells and tissue. Cervical cancer acts as the biggest killer and most preventable cancer in Nepalese women. This paper tries to give a brief overview of cervical cancer screening program in Nepal. Various published and unpublished literatures were obtained and reviewed from international journals, local newspapers and books. All literatures were systematically reviewed and analyzed. Human papilloma virus (HPV) infection is chiefly the reason for cervical cancer. In majority cases, early exposure to sexual intercourse, multiple sex partners and addiction are considered as risk factor. In Developing country like Nepal, screening for cervical cancers is not easily available to people at risk. A vaccine is available which reduces the risk of HPV. The vaccine will be effective if received before the onset of sexual activities 9-13 years. Cervical cancer screening program has many difficulties in terms of limited medical services, difficult geographical terrain creating difficulties in delivering health services. Special programs needs to be designed and delivered to population focusing on reducing burden of cervical cancer. DOI: http://dx.doi.org/10.3126/hprospect.v12i1.8723 Health Prospect: Journal of Public Health Vol.12(1) 2013: 18-20

scholarly journals Pubic Hair Shaving Is Correlated to Vulvar Dysplasia and Inflammation: A Case-Control Study

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Meike Schild-Suhren ◽  
Amr A. Soliman ◽  
Eduard Malik
Keyword(s):  
Case Control Study ◽  
Medical Personnel ◽  
Hpv Infection ◽  
Pubic Hair ◽  
Control Group ◽  
Papilloma Virus ◽  
Cancer Group ◽  
Hpv Status ◽  
Healthy Participants ◽  
Control Study

Objective. The risk factors for vulvar dysplasia and infections are not fully known. In this study, we aimed to investigate the correlation between pubic hair shaving and the occurrence of vulvar inflammation, dysplasia, and cancer. Methods. This study was performed between January 2013 and December 2016 in which a standardized questionnaire concerning genital hair shaving was administered to vulvar dysplasia and cancer patients and healthy participants. The presence of human papilloma virus (HPV) infection and the occurrence of genital inflammation were documented. Results. We recruited 49 patients with vulvar dysplasia or cancer and 234 healthy women as a control group. Smoking, HPV infection, genital inflammation, and complete pubic hair removal were significantly more common in the vulvar dysplasia/cancer group. Pubic hair shaving per se presented a clear association with vulvar dysplasia/cancer. Shaving the labia majora in particular showed also an association. Conclusion. Our findings suggest that partial or complete pubic hair shaving using a razor is correlated with and could be a potential risk factor for the development of genital inflammation, vulvar dysplasia, and malignancies. These results need to be confirmed in larger studies. HPV status and genital inflammation should be documented by medical personnel.

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