scholarly journals Expression of Sucrose Transporters from Vitis vinifera Confer High Yield and Enhances Drought Resistance in Arabidopsis

2020 ◽  
Vol 21 (7) ◽  
pp. 2624
Author(s):  
Yumeng Cai ◽  
Jing Yan ◽  
Wenrui Tu ◽  
Zhefang Deng ◽  
Wenjie Dong ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Drought Resistance ◽  
Transgenic Arabidopsis ◽  
Higher Plants ◽  
Crop Improvement ◽  
High Yield ◽  
Sucrose Transport ◽  
Sucrose Transporters ◽  
Promising Target ◽  
Number Of Leaves

Sucrose is the predominant form of sugar transported from photosynthetic (source) to non-photosynthetic (sink) organs in higher plants relying on the transporting function of sucrose transporters (SUTs or SUCs). Many SUTs have been identified and characterized in both monocots and dicots. However, the function of sucrose transporters (SUTs or SUCs) from Vitis is not clear. As the world’s most planted grape species, Vitis vinifera owns three sucrose transport activity verified SUTs. In this study, we constructed three kinds of VvSUC (Vitis vinifera SUC)-overexpressing transgenic Arabidopsis. VvSUC-overexpressing transgenic Arabidopsis was cultured on sucrose-supplemented medium. VvSUC11- and VvSUC12-overexpressing lines had similar thrived growth phenotypes, whereas the size and number of leaves and roots from VvSUC27-overexpressing lines were reduced compared with that of WT. When plants were cultured in soil, all SUT transgenic seedlings produced more number of leaves and siliques, resulting in higher yield (38.6% for VvSUC12-transformants) than that of WT. Besides, VvSUC27-transformants and VvSUC11-transformants enhanced drought resistance in Arabidopsis, providing a promising target for crop improvement

scholarly journals HUBUNGAN ANTARA KARAKTER VEGETATIF DE NGAN PRODUKSI PATI SAGU BARUQ

2020 ◽  
Vol 20 (4) ◽  
pp. 203
Author(s):  
ELSJE T. TENDA ◽  
. MIFTAHORRACHMAN
Keyword(s):  
Path Analysis ◽  
Indirect Effects ◽  
Selection Criteria ◽  
Crop Improvement ◽  
High Yield ◽  
Font Size ◽  
North Sulawesi ◽  
Number Of Leaves ◽  
Vegetative Characters ◽  
Starch Production

<p><span style="font-size: medium;">ABSTRAK</span></p><p><span style="font-size: medium;"> </span></p><p><span style="font-size: medium;">Sagu baruq merupakan tanaman sumber karbohidrat yang diambil dari batang. Masalah yang dihadapi adalah seleksi produksi pati pada </span><span style="font-size: medium;">setiap pohon. Banyak atau sedikitnya produksi pati akan diketahui setelah pohon ditebang. Tujuan penelitian ini adalah untuk mendapatkan informasi genetik plasma nutfah sagu baruq, terutama untuk mengetahui korelasi antara  karakter-karakter  vegetatif  dengan  produksi  pati,  yang  dapat dimanfaatkan untuk kegiatan seleksi tetua. Salah satu alat yang bisa digunakan untuk mendapatkan informasi ini adalah metode sidik lintas. Karakter yang diamati adalah tinggi batang, jumlah daun, panjang rachis, panjang dan lebar petiol, panjang, lebar, dan jumlah anak daun, serta berat batang, empulur, dan pati. Pengumpulan data dilakukan dengan mengacu pada metode Pusat Penelitian dan Pengembangan Perkebunan tahun 2005. Data yang terkumpul dianalisis koefisien korelasinya untuk mengetahui hubungan langsung dan tidak langsung karakter-karakter vegetatif dengan berat  tepung  sebagai  komponen  hasil.  Perhitungan  koefisien  lintas mengacu pada metode matriks oleh Singh dan Chaudari. Hasil yang diperoleh   menunjukkan   terdapat   enam   karakter   yang   berpengaruh langsung terhadap berat tepung dan dapat dijadikan seleksi peningkatan </span><span style="font-size: medium;">produksi pati sagu baruq di Tabukan Tengah. Sementara itu, di Manganitu, <br /> </span><span style="font-size: medium;">terdapat sembilan karakter yang dapat dijadikan sebagai kriteria seleksi untuk perbaikan tanaman. Selain pengaruh langsung, dihasilkan juga 12 pengaruh tidak langsung yang dapat dipertimbangkan sebagai kriteria seleksi populasi sagu baruq di Tabukan Tengah dan 10 pengaruh tidak langsung untuk Manganitu.</span></p><p><span style="font-size: medium;">Kata kunci:  Arenga macrocarpha,  hubungan, karakter vegetatif, produksi pati</span></p><p><span style="font-size: medium;"> </span></p><p><span style="font-size: medium;"> </span></p><p> </p><p><span style="font-size: medium;">Relationship Between Vegetative Characters an d SagoBaruq Starch Production From Sangihe District </span></p><p><span style="font-size: medium;"><br /></span></p><p><span style="font-size: medium;">ABSTRACT</span></p><p><span style="font-size: medium;"> </span></p><p><span style="font-size: medium;">Baruq sagu is an important source of carbohydrate from the stem, constrain in cultivation was difficulty in selecting high yield plants. The experiment   was   conducted   at   the   District   Tabukan   Tengah   and Manganitu Sangihe Regency, North Sulawesi in May 2012. The purpose of this study was to obtain genetic information for germplasm of sago Baruq,  especially  to  determine  the  correlation  between  vegetative characters  and the production of starch, which  later can be used as the selection of mother palms. One of the tools that can be used to obtain this  information was the method of path analysis. Research methods used  was direct observation in the field. The number of plants observed for  each  site 1 0    trees,  which  were  randomly  selected.  Characters observed were plant height, number of leaves, rachis length, length of petiol, petiol width, length of the leaf,  width of  leaf,  number of leaves, stems weight, pith weight, and weight of starch. The collected data were analyzed  to  determine  the  direct  and  indirect  relationship  between vegetative characters and weight of starch using path analysis. The result showed  that 10  vegetative  characters  of  Baruq  sago  population  in Tabukan  Tengah   District,  Sangihe  regency  produced  six  characters directly influence the character of the starch weight and can be used as selection   criteria   to   increasing   production   of   baruq   sago   starch. Meanwhile, in District Manganitu 9 characters can be used as selection criteria for crop  improvement. In  addition to the direct  effects, 12 indirect effects can be considered as a selection criteria for sago baruq population in District Tabukan Tengah and 10 indirect effects for the population baruq sago in the District Manganitu.</span></p><p><span style="font-size: medium;"> </span></p>Keywords:   Arenga  macrocarpha ,  relationships,  vegetative  character,       starch production

scholarly journals Gene targeting facilitated by engineered sequence-specific nucleases and its potential for applications in crop improvement

2021 ◽  
Author(s):  
Daisuke Miki ◽  
Rui Wang ◽  
Jing Li ◽  
Dali Kong ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Gene Targeting ◽  
Agronomic Traits ◽  
Higher Plants ◽  
Crop Improvement ◽  
Strand Break ◽  
End Joining ◽  
Homology Directed Repair ◽  
Target Dna ◽  
Targeted Gene Editing ◽  
Non Homologous End Joining

Abstract Humans are currently facing the problem of how to ensure that there is enough food to feed all of the world’s population. Ensuring that the food supply is sufficient will likely require the modification of crop genomes to improve their agronomic traits. The development of engineered sequence-specific nucleases (SSNs) paved the way for targeted gene editing in organisms, including plants. SSNs generate a double-strand break (DSB) at the target DNA site in a sequence-specific manner. These DSBs are predominantly repaired via error-prone non-homologous end joining (NHEJ), and are only rarely repaired via error-free homology-directed repair (HDR) if an appropriate donor template is provided. Gene targeting (GT), i.e., the integration or replacement of a particular sequence, can be achieved with combinations of SSNs and repair donor templates. Although its efficiency is extremely low, GT has been achieved in some higher plants. Here, we provide an overview of SSN-facilitated GT in higher plants and discuss the potential of GT as a powerful tool for generating crop plants with desirable features.

scholarly journals Evolutionary and ecological perspectives on the wheat phenotype

2021 ◽  
Vol 288 (1958) ◽  
pp. 20211259
Author(s):  
Victor O. Sadras
Keyword(s):  
Precision Agriculture ◽  
Current Model ◽  
Crop Improvement ◽  
Wheat Yield ◽  
High Yield ◽  
Striking Similarity ◽  
Stabilizing Selection ◽  
Grain Number ◽  
Offspring Number ◽  
Trade Offs

Technologies, from molecular genetics to precision agriculture, are outpacing theory, which is becoming a bottleneck for crop improvement. Here, we outline theoretical insights on the wheat phenotype from the perspective of three evolutionary and ecologically important relations—mother–offspring, plant–insect and plant–plant. The correlation between yield and grain number has been misinterpreted as cause-and-effect; an evolutionary perspective shows a striking similarity between crop and fishes. Both respond to environmental variation through offspring number; seed and egg size are conserved. The offspring of annual plants and semelparous fishes, lacking parental care, are subject to mother–offspring conflict and stabilizing selection. Labile reserve carbohydrates do not fit the current model of wheat yield; they can stabilize grain size, but involve trade-offs with root growth and grain number, and are at best neutral for yield. Shifting the focus from the carbon balance to an ecological role, we suggest that labile carbohydrates may disrupt aphid osmoregulation, and thus contribute to wheat agronomic adaptation. The tight association between high yield and low competitive ability justifies the view of crop yield as a population attribute whereby the behaviour of the plant becomes subordinated within that of the population, with implications for genotyping, phenotyping and plant breeding.

scholarly journals Mutagenic effect of EMS and DES on Tenai (Setaria italica) in M1 generation

1970 ◽  
pp. 06-08
Author(s):  
I. Anittha, L. Mullainathan
Keyword(s):  
Seed Weight ◽  
Quantitative Traits ◽  
Crop Improvement ◽  
Mutagenic Effect ◽  
Mutation Breeding ◽  
Setaria Italica ◽  
Mutagenic Treatment ◽  
Genetic Manipulations ◽  
Number Of Leaves ◽  
Breeding Techniques

Mutation breeding gives better results for crop improvement through genetic manipulations when compared  to  other conventional breeding techniques. The present work focused in order to find out the effect of chemical mutagens; EMS and DES on Setaria italica in M1 generation. The seeds of Tenai, variety CO(Te)7 treated with different concentration of EMS and DES. The LD50 was observed at 30mM in EMS and 40mM in DES. Selection studies were conducted to improve the yield and to generate genetic variability in different quantitative traits such as days to first bloom, plant height, number of leaves, number of nodes, length and breadth of ear head, 1000 seed weight and yield per plant. The results revealed that, all the parameters were decreased with increasing concentration in both EMS and DES, while days to first bloom was increasing with increasing concentration. According to the result all the parameters  studied  shows a negative direction towards crop improvement in M1 generation because of the stress caused by mutagenic treatment.

Sucrose Transport in Higher Plants

1997 ◽  
pp. 41-71 ◽  
Author(s):  
John M. Ward ◽  
Christina Kühn ◽  
Mechthild Tegeder ◽  
Wolf B. Frommer
Keyword(s):  
Higher Plants ◽  
Sucrose Transport

scholarly journals Factors controlling drought resistance in grapevine ( Vitis vinifera , chardonnay): application of a new micro CT method to assess functional embolism resistance

2020 ◽  
Vol 107 (4) ◽  
pp. 618-627
Author(s):  
R. Brandon Pratt ◽  
Viridiana Castro ◽  
Jaycie C. Fickle ◽  
Angela Madsen ◽  
Anna L. Jacobsen
Keyword(s):  
Vitis Vinifera ◽  
Drought Resistance ◽  
Micro Ct ◽  
Embolism Resistance

scholarly journals Gephebase, a database of genotype–phenotype relationships for natural and domesticated variation in Eukaryotes

2019 ◽  
Vol 48 (D1) ◽  
pp. D696-D703
Author(s):  
Virginie Courtier-Orgogozo ◽  
Laurent Arnoult ◽  
Stéphane R Prigent ◽  
Séverine Wiltgen ◽  
Arnaud Martin
Keyword(s):  
Target Genes ◽  
Crop Improvement ◽  
Carotenoid Content ◽  
Phenotypic Change ◽  
Candidate Gene Approach ◽  
Global Trends ◽  
Research Fields ◽  
Promising Target ◽  
Mutant Phenotypes ◽  
Meta Analyses

Abstract Gephebase is a manually-curated database compiling our accumulated knowledge of the genes and mutations that underlie natural, domesticated and experimental phenotypic variation in all Eukaryotes—mostly animals, plants and yeasts. Gephebase aims to compile studies where the genotype–phenotype association (based on linkage mapping, association mapping or a candidate gene approach) is relatively well supported. Human clinical traits and aberrant mutant phenotypes in laboratory organisms are not included and can be found in other databases (e.g. OMIM, OMIA, Monarch Initiative). Gephebase contains more than 1700 entries. Each entry corresponds to an allelic difference at a given gene and its associated phenotypic change(s) between two species or two individuals of the same species, and is enriched with molecular details, taxonomic information, and bibliographic information. Users can easily browse entries and perform searches at various levels using boolean operators (e.g. transposable elements, snakes, carotenoid content, Doebley). Data is exportable in spreadsheet format. This database allows to perform meta-analyses to extract global trends about the living world and the research fields. Gephebase should also help breeders, conservationists and others to identify promising target genes for crop improvement, parasite/pest control, bioconservation and genetic diagnostic. It is freely available at www.gephebase.org.

Resistance to Drought and Salinity: Avenues for Improvement

1986 ◽  
Vol 13 (1) ◽  
pp. 191 ◽  
Author(s):  
JB Passioura
Keyword(s):  
Drought Resistance ◽  
Dry Matter ◽  
Random Search ◽  
The Other ◽  
Yield Potential ◽  
High Yield ◽  
Optimal Time ◽  
Middle Ground ◽  
Wide Crosses ◽  
High Yield Potential

Various techniques for improving the resistance of plants to drought or salinity are discussed in relation to a notional spectrum ranging from rationality to random search. The rational end of the spectrum is exemplified by the control of phenology so that flowering occurs at an optimal time; the random end by wide crosses, such as that between wheat and Elytrigia, which give the remote hope that the high yield-potential of one will combine with the toughness of the other. Most of the techniques occupy the middle ground and involve selecting for characters whose protagonists have strong, but often poorly articulated, hunches that the characters will confer resistance to drought or salinity on the plants possessing them. For drought resistance of grain crops, the possible worth of many of these characters is discussed in relation to their likely impact on improving a crop's ability to (a) extract water from the soil, (b) produce dry matter given a limited water supply, and (c) convert dry matter into grain.

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