scholarly journals Bioactive Glass (BG) ICIE16 Shows Promising Osteogenic Properties Compared to Crystallized 45S5-BG

2020 ◽  
Vol 21 (5) ◽  
pp. 1639 ◽  
Author(s):  
Fabian Westhauser ◽  
Frederike Hohenbild ◽  
Marcela Arango-Ospina ◽  
Sarah I. Schmitz ◽  
Sebastian Wilkesmann ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Biological Properties ◽  
Cellular Level ◽  
Human Mesenchymal Stromal Cells ◽  
Ecm Proteins ◽  
Genes Encoding ◽  
Increased Activity ◽  
Stimulation Of

The ICIE16-bioactive glass (BG) (48.0 SiO2, 6.6 Na2O, 32.9 CaO, 2.5 P2O5, 10.0 K2O (wt %)) has been developed as an alternative to 45S5-BG, the original BG composition (45.0 SiO2, 24.5 Na2O, 24.5 CaO, 6.0 P2O5 (wt %)), with the intention of broadening the BG sintering window while maintaining bioactivity. Because there is a lack of reports on ICIE16-BG biological properties, the influence of ICIE16-BG on viability, proliferation, and osteogenic differentiation of human mesenchymal stromal cells (MSCs) was evaluated in direct comparison to 45S5-BG in this study. The BGs underwent heat treatment similar to that which is required in order to fabricate scaffolds by sintering, which resulted in crystallization of 45S5-BG (45S5-CBG) while ICIE16 remained amorphous. Granules based on both BGs were biocompatible, but ICIE16-BG was less harmful to cell viability, most likely due to a more pronounced pH alkalization in the 45S5-CBG group. ICIE16-BG outperformed 45S5-CBG in terms of osteogenic differentiation at the cellular level, as determined by the increased activity of alkaline phosphatase. However, granules from both BGs were comparable regarding the stimulation of expression levels of genes encoding for osseous extracellular matrix (ECM) proteins. The addition of therapeutically active ions to ICIE16-BG might further improve its ability to stimulate ECM production and should be investigated in upcoming studies.

scholarly journals Impact of Zinc- or Copper-Doped Mesoporous Bioactive Glass Nanoparticles on the Osteogenic Differentiation and Matrix Formation of Mesenchymal Stromal Cells

Materials ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 1864
Author(s):  
Fabian Westhauser ◽  
Simon Decker ◽  
Qaisar Nawaz ◽  
Felix Rehder ◽  
Sebastian Wilkesmann ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Osteogenic Differentiation ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Related Gene ◽  
Future Studies ◽  
Positive Effects ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Mesoporous Bioactive Glass

Mesoporous bioactive glass nanoparticles (MBGNs) have gained relevance in bone tissue engineering, especially since they can be used as vectors for therapeutically active ions like zinc (Zn) or copper (Cu). In this study, the osteogenic properties of the ionic dissolution products (IDPs) of undoped MBGNs (composition in mol%: 70 SiO2, 30 CaO) and MBGNs doped with 5 mol% of either Zn (5Zn-MBGNs) or Cu (5Cu-MBGNs; compositions in mol%: 70 SiO2, 25 CaO, 5 ZnO/CuO) on human bone marrow-derived mesenchymal stromal cells were evaluated. Extracellular matrix (ECM) formation and calcification were assessed, as well as the IDPs’ influence on viability, cellular osteogenic differentiation and the expression of genes encoding for relevant members of the ECM. The IDPs of undoped MBGNs and 5Zn-MBGNs had a comparable influence on cell viability, while it was enhanced by IDPs of 5Cu-MBGNs compared to the other MBGNs. IDPs of 5Cu-MBGNs had slightly positive effects on ECM formation and calcification. 5Zn-MBGNs provided the most favorable pro-osteogenic properties since they increased not only cellular osteogenic differentiation and ECM-related gene expression but also ECM formation and calcification significantly. Future studies should analyze other relevant properties of MBGNs, such as their impact on angiogenesis.

scholarly journals Rationale for Processing of a Mg-Zn-Ca Alloy by Equal-Channel Angular Pressing for Use in Biodegradable Implants for Osteoreconstruction

Crystals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1381
Author(s):  
Natalia S. Martynenko ◽  
Natalia Yu. Anisimova ◽  
Olga V. Rybalchenko ◽  
Mikhail V. Kiselevskiy ◽  
Georgy Rybalchenko ◽  
...  
Keyword(s):  
Equal Channel Angular Pressing ◽  
Stromal Cells ◽  
Orthopedic Implants ◽  
Biodegradable Implants ◽  
Promising Candidate ◽  
Human Mesenchymal Stromal Cells ◽  
Angular Pressing ◽  
Cell Adhesion And Proliferation ◽  
Stimulation Of

Widespread use of Mg-Zn-Ca alloys in clinical orthopedic practice requires improvement of their mechanical properties—in particular, ductility—and enhancement of their bioactivity for accelerated osteoreconstruction. The alloy was studied in two structural states: after homogenization and after equal-channel angular pressing. Immersion and potentiodynamic polarization tests showed that the corrosion rate of the alloy was not increased by deformation. The mass loss in vivo was also statistically insignificant. Furthermore, it was found that deformation did not compromise the biocompatibility of the alloy and did not have any significant effect on cell adhesion and proliferation. However, an extract of the alloy promoted the alkaline phosphatase activity of human mesenchymal stromal cells, which indicates osteogenic stimulation of cells. The osteoinduction of the deformed alloy significantly exceeded that of the homogenized one. Based on the results of this work, it can be concluded that the alloy Mg-1%Zn-0.3%Ca modified by equal-channel angular pressing is a promising candidate for the manufacture of biodegradable orthopedic implants since it stimulates osteogenic differentiation and has greater ductility, which provides it with a competitive advantage in comparison with the homogenized state.

Stimulation of osteogenic differentiation in bone marrow stromal cells via Wnt/β-catenin pathway by Qili Jiegu-containing serum

2018 ◽  
Vol 103 ◽  
pp. 1664-1668
Author(s):  
Han-Bing Song ◽  
Ying Jiang ◽  
Jia-Xing Liu ◽  
Ge-Qiang Wang ◽  
Da-Peng Zhang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Stimulation Of
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