scholarly journals Plasma Membrane Ca2+ Permeable Mechanosensitive Channel OsDMT1 Is Involved in Regulation of Plant Architecture and Ion Homeostasis in Rice

2020 ◽  
Vol 21 (3) ◽  
pp. 1097
Author(s):  
Jiayan Liang ◽  
Yan He ◽  
Qiuxin Zhang ◽  
Wenyi Wang ◽  
Zemin Zhang
Keyword(s):  
Plasma Membrane ◽  
Crop Production ◽  
Plant Architecture ◽  
Ion Homeostasis ◽  
Mechanosensitive Channel ◽  
Production Plant ◽  
Transcript Expression ◽  
Calcium Dependent ◽  
Protein Levels ◽  
Rice Mutant

Plant architecture is an important factor for crop production. Plant height, tiller pattern, and panicle morphology are decisive factors for high grain yield in rice. Here, we isolated and characterized a T-DNA insertion rice mutant Osdmt1 (Oryza sativa dwarf and multi-tillering1) that exhibited a severe dwarf phenotype and multi-tillering. Molecular cloning revealed that DMT1 encodes a plasma membrane protein that was identified as a putative Ca2+ permeable mechanosensitive channel. The transcript expression level was significantly higher in the dmt1 mutant compared to wild type (WT). Additionally, the dmt1 homozygous mutant displayed a stronger phenotype than that of the WT and heterozygous seedlings after gibberellic acid (GA) treatment. RNA-seq and iTRAQ-based proteome analyses were performed between the dmt1 mutant and WT. The transcriptome profile revealed that several genes involved in GA and strigolactone (SL) biosyntheses were altered in the dmt1 mutant. Ca2+ and other ion concentrations were significantly enhanced in the dmt1 mutant, suggesting that DMT1 contributes to the accumulation of several ions in rice. Moreover, several EF-hand Ca2+ sensors, including CMLs (CaM-like proteins) and CDPKs (calcium-dependent protein kinases), displayed markedly altered transcript expression and protein levels in the dmt1 mutant. Overall, these findings aid in the elucidation of the multiply regulatory roles of OsDMT1/OsMCA1 in rice.

scholarly journals Calcium-dependent fusion of the plasma membrane fraction from human neutrophils with liposomes

1990 ◽  
Vol 1025 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Joseph W. Francis ◽  
James E. Smolen ◽  
Kenneth J. Balazovich ◽  
Rebecca R. Sandborg ◽  
Laurence A. Boxer
Keyword(s):  
Plasma Membrane ◽  
Membrane Fraction ◽  
Human Neutrophils ◽  
Plasma Membrane Fraction ◽  
Calcium Dependent

scholarly journals Cation-chloride cotransporters, Na/K pump, and channels in cell water and ions regulation: in silico and experimental studies of the U937 cells under stopping the pump and during RVD

2021 ◽  
Author(s):  
Alexey A Vereninov ◽  
Valentina Yurinskaya
Keyword(s):  
Plasma Membrane ◽  
Cell Membrane ◽  
Real Time ◽  
Intracellular Signaling ◽  
Ion Homeostasis ◽  
Regulatory Volume Decrease ◽  
Experimental Studies ◽  
U937 Cells ◽  
Ionic Homeostasis ◽  
Monovalent Ions

Cation-coupled chloride cotransporters play a key role in generating the Cl− electrochemical gradient on the cell membrane which is important for regulation of many cellular processes. However, the cooperation of transporters and channels of the plasma membrane in holding the ionic homeostasis of the whole cell remains poorly characterized because of the lack of a suitable tool for its computation. Our software successfully predicted in real-time changes in the ion homeostasis of U937 cells after stopping the Na/K pump, but so far considered the model with only NC cotransporter. Here the model with all main types of cotransporters is used in computation of the rearrangements of ionic homeostasis due to stopping the pump and associated with the regulatory volume decrease (RVD) of cells swollen in hypoosmolar medium. The parameters obtained for the real U937 cells are used. Successful prediction of changes in ion homeostasis in real-time after stopping the pump using the model with all major cotransporters indicates that the model is reliable. Using this model for analysis RVD showed that there is a "physical" RVD, associated with the time-dependent changes in electrochemical ion gradients, but not with alteration of channels and transporters of the plasma membrane that should be considered in studies of truly active regulatory processes mediated by the intracellular signaling network. The developed software can be useful for calculation of the balance of the partial unidirectional fluxes of monovalent ions across the cell membrane of various cells under various conditions.

Calcium regulation of the contractile state of isolated mammalian fibroblast cytoplasm

1975 ◽  
Vol 18 (2) ◽  
pp. 241-256
Author(s):  
C.S. Izzard ◽  
S.L. Izzard
Keyword(s):  
Plasma Membrane ◽  
Striated Muscle ◽  
Calcium Regulation ◽  
Threshold Concentration ◽  
Free Access ◽  
Contractile State ◽  
Relaxing Solution ◽  
Calcium Dependent ◽  
Rigor Solution

Calcium-dependent contractions have been induced in fresh, naked cytoplasm of L-929 fibroblasts using physiological solutions (rigor, relaxing and contracting) similar to those designed to control the contractile state of vertebrate striated muscle. Free access of solutions to the cytoplasm was achieved by popping and stripping the plasma membrane from cells using 7–10 strokes of a Dounce homogenizer. Contracting solution (free Ca2+ 7 X 10(−5) M; with added MgATP) applied locally from a micropipette to cells popped in rigor (free Ca2+ less than 10(−8) M) or relaxing (free Ca2+ less than 10(−8) M; with added MgATP) solutions induced symmetrical contractions of unstretched cytoplasm and directional shortening of stretched cytoplasm. The contractions produced 12–18% shortenings and were complete in 1–3 s. The cytoplasm could be cycled repeatedly through the contracted state from the relaxed state. Exogenous MgATP was required for the Ca2+-dependent contractions. At low free Ca2+ concentrations (less than 10(−8) M), MgATP had a marked plasticizing effect on the cytoplasm. Thus cytoplasm prepared in relaxing solution was less cohesive and more easily deformed than cytoplasm prepared in rigor solution. When induced to contract, relaxed cytoplasm showed a loss of plasticity. Using this criterion, the threshold concentration of free Ca2+ for contraction was determined to lie between 7 X 10(−8) M and 5 X 10(−7) M.

Application of Extremophiles in Sustainable Agriculture

2022 ◽  
pp. 233-250
Author(s):  
Julius Eyiuche Nweze ◽  
Justus Amuche Nweze ◽  
Shruti Gupta
Keyword(s):  
Sustainable Agriculture ◽  
Bioactive Compounds ◽  
Environmental Conditions ◽  
Agricultural Production ◽  
Crucial Role ◽  
Crop Production ◽  
Agricultural Practices ◽  
Production Plant ◽  
Physiological Conditions ◽  
Sustainable Agricultural Practices

With the increasing demands for foods and other agriculture-based products, sustainable agricultural practices are the cornerstone for improving low-input agricultural production. In contrast to crop production, plant-microorganism interaction (PMI) plays a crucial role. PMI significantly raises productivity as well as maintaining the overall health of the crop. During harsh and extreme physiological conditions, plant-associated extremophilic microbes (PAEM) are known to contribute to crop production, survivability, and fitness. Thus, the application of extremophiles either in the form of biofertilizer or biopesticides is highly beneficial. Extremophiles have been adapted to withstand diverse harsh environmental conditions. They possess unique mechanisms at the molecular level to produce enormous potential extremozymes and bioactive compounds. Consequently, extremophiles represent the foundation of efficient and sustainable agriculture. This chapter introduces the significance and application of plant-associated extremophilic microbes in sustainable agriculture.

scholarly journals Cell Secretion: Current Structural and Biochemical Insights

2010 ◽  
Vol 10 ◽  
pp. 2054-2069 ◽  
Author(s):  
Saurabh Trikha ◽  
Elizabeth C. Lee ◽  
Aleksandar M. Jeremic
Keyword(s):  
Plasma Membrane ◽  
Secretory Vesicles ◽  
Intercellular Signaling ◽  
Cell Secretion ◽  
Membrane Structures ◽  
Calcium Dependent ◽  
Cell Plasma ◽  
Membrane Bound ◽  
And Control ◽  
Fusion Pores

Essential physiological functions in eukaryotic cells, such as release of hormones and digestive enzymes, neurotransmission, and intercellular signaling, are all achieved by cell secretion. In regulated (calcium-dependent) secretion, membrane-bound secretory vesicles dock and transiently fuse with specialized, permanent, plasma membrane structures, called porosomes or fusion pores. Porosomes are supramolecular, cup-shaped lipoprotein structures at the cell plasma membrane that mediate and control the release of vesicle cargo to the outside of the cell. The sizes of porosomes range from 150nm in diameter in acinar cells of the exocrine pancreas to 12nm in neurons. In recent years, significant progress has been made in our understanding of the porosome and the cellular activities required for cell secretion, such as membrane fusion and swelling of secretory vesicles. The discovery of the porosome complex and the molecular mechanism of cell secretion are summarized in this article.

scholarly journals Transglutaminase 2 Induces Deficits in Social Behavior in Mice

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Amanda Crider ◽  
Talisha Davis ◽  
Anthony O. Ahmed ◽  
Lin Mei ◽  
Anilkumar Pillai
Keyword(s):  
Social Behavior ◽  
Er Stress ◽  
Transglutaminase 2 ◽  
Underlying Mechanism ◽  
Autism Spectrum ◽  
Postmortem Brain ◽  
Postmortem Human Brain ◽  
Calcium Dependent ◽  
Human Postmortem Brain ◽  
Protein Levels

Impairments in social behavior are highly implicated in many neuropsychiatric disorders. Recent studies indicate a role for endoplasmic reticulum (ER) stress in altering social behavior, but the underlying mechanism is not known. In the present study, we examined the role of transglutaminase 2 (TG2), a calcium-dependent enzyme known to be induced following ER stress, in social behavior in mice. ER stress induced by tunicamycin administration increased TG2 protein levels in the mouse prefrontal cortex (PFC). PFC-specific inhibition of TG2 attenuated ER stress-induced deficits in social behavior. Conversely, overexpression of TG2 in the PFC resulted in social behavior impairments in mice. In addition, systemic administration of cysteamine, a TG2 inhibitor, attenuated social behavior deficits. Our preliminary findings using postmortem human brain samples found increases in TG2 mRNA and protein levels in the middle frontal gyrus of subjects with autism spectrum disorder. These findings in mice and human postmortem brain samples identify changes in TG2 activity in the possible dysregulation of social behavior.

scholarly journals How does nitrogen shape plant architecture?

2020 ◽  
Vol 71 (15) ◽  
pp. 4415-4427 ◽  
Author(s):  
Le Luo ◽  
Yali Zhang ◽  
Guohua Xu
Keyword(s):  
Cell Division ◽  
Crop Production ◽  
Plant Architecture ◽  
Reproductive Organs ◽  
N Fertilization ◽  
High Yield ◽  
Shoot Branching ◽  
Shoot Height ◽  
Plant Nitrogen ◽  
N Supply

Abstract Plant nitrogen (N), acquired mainly in the form of nitrate and ammonium from soil, dominates growth and development, and high-yield crop production relies heavily on N fertilization. The mechanisms of root adaptation to altered supply of N forms and concentrations have been well characterized and reviewed, while reports concerning the effects of N on the architecture of vegetative and reproductive organs are limited and are widely dispersed in the literature. In this review, we summarize the nitrate and amino acid regulation of shoot branching, flowering, and panicle development, as well as the N regulation of cell division and expansion in shaping plant architecture, mainly in cereal crops. The basic regulatory steps involving the control of plant architecture by the N supply are auxin-, cytokinin-, and strigolactone-controlled cell division in shoot apical meristem and gibberellin-controlled inverse regulation of shoot height and tillering. In addition, transport of amino acids has been shown to be involved in the control of shoot branching. The N supply may alter the timing and duration of the transition from the vegetative to the reproductive growth phase, which in turn may affect cereal crop architecture, particularly the structure of panicles for grain yield. Thus, proper manipulation of N-regulated architecture can increase crop yield and N use efficiency.

scholarly journals Comparative Transcriptome Combined with Proteome Analyses Revealed Key Factors Involved in Alfalfa (Medicago sativa) Response to Waterlogging Stress

2019 ◽  
Vol 20 (6) ◽  
pp. 1359 ◽  
Author(s):  
Ningbo Zeng ◽  
Zhijian Yang ◽  
Zhifei Zhang ◽  
Longxing Hu ◽  
Liang Chen
Keyword(s):  
Medicago Sativa ◽  
Molecular Mechanism ◽  
Crop Production ◽  
Expression Profiles ◽  
Resistance Breeding ◽  
Ethylene Response Factor ◽  
Proline Metabolism ◽  
Comparative Transcriptome ◽  
Waterlogging Stress ◽  
Protein Levels

Alfalfa (Medicago sativa) is the most widely grown and most important forage crop in the world. However, alfalfa is susceptible to waterlogging stress, which is the major constraint for its cultivation area and crop production. So far, the molecular mechanism of alfalfa response to the waterlogging is largely unknown. Here, comparative transcriptome combined with proteomic analyses of two cultivars (M12, tolerant; M25, sensitive) of alfalfa showing contrasting tolerance to waterlogging were performed to understand the mechanism of alfalfa in response to waterlogging stress. Totally, 748 (581 up- and 167 down-regulated) genes were differentially expressed in leaves of waterlogging-stressed alfalfa compared with the control (M12_W vs. M12_CK), whereas 1193 (740 up- and 453 down-regulated) differentially abundant transcripts (DATs) were detected in the leaves of waterlogging-stressed plants in comparison with the control plants (M25_W vs. M25_CK). Furthermore, a total of 187 (122 up- and 65 down-regulated) and 190 (105 up- and 85 down-regulated) differentially abundant proteins (DAPs) were identified via isobaric tags for relative and absolute quantification (iTRAQ) method in M12_W vs. M12_CK and M25_W vs. M25_CK comparison, respectively. Compared dataset analysis of proteomics and transcriptomics revealed that 27 and eight genes displayed jointly up-regulated or down-regulated expression profiles at both mRNA and protein levels in M12_W vs. M12_CK comparison, whereas 30 and 27 genes were found to be co-up-regulated or co-down-regulated in M25_W vs. M25_CK comparison, respectively. The strongly enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways for co-up-regulated genes at mRNA and protein levels in M12_W vs. M12_CK comparison were ‘Amino sugar and nucleotide sugar metabolism’, ‘Arginine and proline metabolism’ and ‘Starch and sucrose metabolism’, whereas co-up-regulated protein-related pathways including ‘Arginine and proline metabolism’ and ‘Valine, leucine and isoleucine degradation’ were largely enriched in M25_W vs. M25_CK comparison. Importantly, the identified genes related to beta-amylase, Ethylene response Factor (ERF), Calcineurin B-like (CBL) interacting protein kinases (CIPKs), Glutathione peroxidase (GPX), and Glutathione-S-transferase (GST) may play key roles in conferring alfalfa tolerance to waterlogging stress. The present study may contribute to our understanding the molecular mechanism underlying the responses of alfalfa to waterlogging stress, and also provide important clues for further study and in-depth characterization of waterlogging-resistance breeding candidate genes in alfalfa.

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