scholarly journals Dexamethasone Treatment Increases the Intracellular Calcium Level Through TRPV6 in A549 Cells

2020 ◽  
Vol 21 (3) ◽  
pp. 1050
Author(s):  
Bo-Hui Jeon ◽  
Yeong-Min Yoo ◽  
Eui-Man Jung ◽  
Eui-Bae Jeung
Keyword(s):  
Intracellular Calcium ◽  
Calcium Level ◽  
Transient Receptor Potential ◽  
Calcium Influx ◽  
Mrna Level ◽  
A549 Cells ◽  
Mrna Levels ◽  
Intracellular Calcium Level ◽  
Mucin Genes ◽  
Airway Mucin

This study investigated the effect of dexamethasone (DEX) on intracellular calcium levels and the expressions of transient receptor potential cation channel subcomponent V member 6 (TRPV6), sodium-calcium exchanger 1 (NCX1), and plasma membrane calcium ATPase 1 (PMCA1) in A549 cells. The intracellular calcium level, by using the calcium indicator pGP-CMV-GCaMP6f, increased following DEX treatment for 6, 12, and 24 h in A549 cells. In addition, Rhod-4 assay after DEX treatment for 24 h showed that DEX increased the level of intracellular calcium. The expression of the calcium influx TRPV6 gene significantly increased, whereas the expressions of the calcium outflow NCX1 and PMCA1 genes significantly decreased with DEX treatment. The mRNA levels of surfactant protein genes SFTPA1, SFTPB, SFTPC, and SFTPD and the secreted airway mucin genes MUC1 and MUC5AC were investigated by treating cells with DEX. The DEX treatment decreased the mRNA levels of SFTPA1 and SFTPB but increased the mRNA levels of SFTPC and SFTPD. The MUC1 mRNA level was increased by DEX treatment, whereas MUC5AC mRNA was significantly decreased. These results indicate that DEX influences the intracellular calcium level through TRPV6, and affects pulmonary surfactant genes and secreted airway mucin genes in A549 cells.

MEASUREMENT OF PLATELET IONIZED CALCIUM IN PATIENTS WITH MYELOPROLIFERATIVE DISORDERS BY AEQUORIN METHOD

1987 ◽  
Author(s):  
T Fuzimoto ◽  
K Fuzimura ◽  
A Kuramoto
Keyword(s):  
Intracellular Calcium ◽  
Calcium Level ◽  
Arrival Time ◽  
Calcium Influx ◽  
Myeloproliferative Disorders ◽  
Myelogenous Leukemia ◽  
Membrane Glycoprotein ◽  
Bleeding Tendency ◽  
Platelet Counts ◽  
Intracellular Calcium Level

In myeloproliferative disorders(MPD), bleeding tendency and thrombosis are encountered occasionally in the presence of high platelet counts. It has been reported that there are some abnormalities of membrane glycoprotein or arachidonate metabolism in platelets of MPD. We measured the intracellular change of calcium levels[Cai2+] after stimulation in platelets of the patients with MPD by Aequorin method. Eleven cases of chronic myelogenous leukemia(CML), 7 cases of polycythemia vera(PV), 4 cases of essential thrombocythemiaCET^ and 12 normal adults were studied, and as stimulators 0.5 U/ml thrombin and 2.5 μg/ml collagen were used. In the patients with CML, PV and ET, maximum intracellular calcium level was significantly lower and the reaction period reaching to the peak calcium level(the arrival time) was significantly prolonged than the normals after thrombin stimulation. On collagen stimulation, maximum[Cai2+] level in patient with CML and PV was significantly lower and the arrival time in patient with CML and ET was significantly prolonged than the normals. No correlation was found between maximum [Cai2+] level and maximum aggregation rate or platelet counts in those patients. The increasing rate of intracellular calcium levels after stimulation with A23187 in the presence of various concentration of extracellular calcium was proved lower in CML than in the normal. [45Ca2+] uptake rate into the CML platelets after thrombin stimulation showed lower rate compaired with normals. These results suggest that platelets in patients with MPD have some abnormalities of calcium influx mechanism. We already reported that platelet membrane glycoprotein (GP)IIIa was increased significantly in MPD platelets. The study is going to examine the relationship between this membrane abnormality and impaired calcium influx in MPD.

Effect of Neuroregulators on the Intracellular Calcium Level in the Outer Hair Cell Isolated from the Guinea Pig

ORL ◽  
1991 ◽  
Vol 53 (2) ◽  
pp. 78-81 ◽  
Author(s):  
Katsuhisa Ikeda ◽  
Yoshitaka Saito ◽  
Akinori Nishiyama ◽  
Tomonori Takasaka
Keyword(s):  
Guinea Pig ◽  
Hair Cell ◽  
Intracellular Calcium ◽  
Calcium Level ◽  
Outer Hair Cell ◽  
Intracellular Calcium Level

Lowering the stimulated free intracellular calcium level in platelets in the refractory state

1988 ◽  
Vol 106 (6) ◽  
pp. 1699-1702
Author(s):  
E. Ya. Pozin ◽  
E. G. Popov ◽  
I. Yu. Gavrilov ◽  
Z. A. Gabbasov ◽  
S. N. Krotov
Keyword(s):  
Intracellular Calcium ◽  
Calcium Level ◽  
Intracellular Calcium Level ◽  
Free Intracellular Calcium ◽  
Refractory State

scholarly journals The effect of intracellular calcium level regulators on the synthesis of pollen tube callose in Oenothera biennis L.

2014 ◽  
Vol 58 (2) ◽  
pp. 199-210 ◽  
Author(s):  
Elżbieta Bednarska
Keyword(s):  
Pollen Tube ◽  
Calcium Channels ◽  
Intracellular Calcium ◽  
Calcium Antagonists ◽  
Calcium Level ◽  
Ruthenium Red ◽  
Tube Growth ◽  
Oenothera Biennis ◽  
A 23187 ◽  
Intracellular Calcium Level

It is shown that callose synthesis in the <em>Oenothera biennis </em>pollen tube is regulated by the endogenous Ca<sup>2+</sup> level. Calcium antagonists reduced the amount of callose in the wall above the tip of the pollen tube (Verapamil - calcium channels blocker) and at the tube tip after stopping tube growth (La<sup>3+</sup> - a Ca<sup>2+</sup> substitute). Ruthenium red and ionophore A 23187, which raise the Ca 21 level in the cytoplasm, induced callose synthesis at the tip of pollen tube.

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