scholarly journals Effects of Cold Jet Atmospheric Pressure Plasma on the Structural Characteristics and Immunoreactivity of Celiac-Toxic Peptides and Wheat Storage Proteins

2020 ◽  
Vol 21 (3) ◽  
pp. 1012
Author(s):  
Fusheng Sun ◽  
Xiaoxue Xie ◽  
Yufan Zhang ◽  
Jiangwei Duan ◽  
Mingyu Ma ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Storage Proteins ◽  
Structural Characteristics ◽  
Atmospheric Pressure Plasma ◽  
High Energy ◽  
Backbone Cleavage ◽  
Excited Atoms ◽  
Wheat Storage Proteins ◽  
Toxic Peptides

The present research reported the effects of structural properties and immunoreactivity of celiac-toxic peptides and wheat storage proteins modified by cold jet atmospheric pressure (CJAP) plasma. It could generate numerous high-energy excited atoms, photons, electrons, and reactive oxygen and nitrogen species, including O3, H2O2, •OH, NO2− and NO3− etc., to modify two model peptides and wheat storage proteins. The Orbitrap HR-LC-MS/MS was utilized to identify and quantify CJAP plasma-modified model peptide products. Backbone cleavage of QQPFP and PQPQLPY at specific proline and glutamine residues, accompanied by hydroxylation at the aromatic ring of phenylalanine and tyrosine residues, contributed to the reduction and modification of celiac-toxic peptides. Apart from fragmentation, oxidation, and agglomeration states were evaluated, including carbonyl formation and the decline of γ-gliadin. The immunoreactivity of gliadin extract declined over time, demonstrating a significant decrease by 51.95% after 60 min of CJAP plasma treatment in vitro. The CJAP plasma could initiate depolymerization of gluten polymer, thereby reducing the amounts of large-sized polymers. In conclusion, CJAP plasma could be employed as a potential technique in the modification and reduction of celiac-toxic peptides and wheat storage proteins.

scholarly journals Reducing Immunoreactivity of Gliadins and Coeliac-Toxic Peptides Using Peptidases from L. acidophilus 5e2 and A. niger

Catalysts ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 923
Author(s):  
Bartosz Brzozowski ◽  
Katarzyna Stasiewicz ◽  
Mateusz Ostolski ◽  
Marek Adamczak
Keyword(s):  
Lactobacillus Acidophilus ◽  
Storage Proteins ◽  
Prolyl Endopeptidase ◽  
Bread Making ◽  
Initial Value ◽  
Histocompatibility Complex ◽  
Wheat Storage Proteins ◽  
Toxic Peptides ◽  
Hydrolysis Of ◽  
Hla Dq2

Wheat storage proteins and products of their hydrolysis may cause coeliac sprue in genetically predisposed individuals with high expression of main histocompatibility complex HLA-DQ2 or DQ8, since by consuming wheat, they become exposed to proline- (P) and glutamine (Q)-rich gluten. In bread-making, the hydrolysis of gliadins and coeliac-toxic peptides occurs with varied efficiency depending on the fermentation pH and temperature. Degradation of gliadins catalysed by Lactobacillus acidophilus 5e2 peptidases and a commercial prolyl endopeptidase synthesised by A. niger, carried out at pH 4.0 and 37 °C, reduces the gliadin concentration over 110-fold and decreases the relative immunoreactivity of the hydrolysate to 0.9% of its initial value. Hydrolysis of coeliac-toxic peptides: LGQQQPFPPQQPY (P1) and PQPQLPYPQPQLP (P2) under the same conditions occurs with the highest efficiency, reaching 99.8 ± 0.0% and 97.5 ± 0.1%, respectively. The relative immunoreactivity of peptides P1 and P2 was 0.8 ± 0.0% and 3.2 ± 0.0%, respectively. A mixture of peptidases from L. acidophilus 5e2 and A. niger may be used in wheat sourdough fermentation to reduce the time needed for degradation of proteins and products of their hydrolysis.

scholarly journals The Effects of Atmospheric Pressure Argon Plasma Treated Bovine Bone Substitute on Bone Regeneration

Coatings ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 790
Author(s):  
Jong-Ju Ahn ◽  
Ji-Hyun Yoo ◽  
Eun-Bin Bae ◽  
Gyoo-Cheon Kim ◽  
Jae Joon Hwang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Atmospheric Pressure ◽  
Bone Substitute ◽  
Argon Plasma ◽  
Atmospheric Pressure Plasma ◽  
Regeneration Ability ◽  
Bovine Bone ◽  
Significant Difference

This study was undertaken to compare new bone formation between non-expired and expired bovine-derived xenogeneic bone substitute (expired, out-of-use period) and to evaluate the efficacy of argon (Ar)-based atmospheric pressure plasma (APP) treatment on expired bone substitute in rat calvarial defect. The groups were divided into (1) Non/Expired group (Using regular xenografts), (2) Expired group (Using expired xenografts), and (3) Ar/Expired group (Using Ar-based APP treated expired xenografts). Surface observation and cell experiments were performed in vitro. Twelve rats were used for in vivo experiment and the bony defects were created on the middle of the cranium. The bone substitute of each group was implanted into the defective site. After 4 weeks, all the rats were sacrificed, and the volumetric, histologic, and histometric analyses were performed. In the results of osteogenic differentiation and mineralization, Non/Expired and Ar/Expired groups were significantly higher than Expired group (p < 0.05). However, there was no significant difference between groups in the animal study (p > 0.05). Within the limitations of this study, the surface treatment of Ar-based APP has a potential effect on the surface modification of bone grafts. However, there was no significant difference in bone regeneration ability between groups in vivo; thus, studies on APP to enhance bone regeneration should be carried out in the future.

Application of Cold Atmospheric Pressure Plasmas for Biological Tissue Treatment

2015 ◽  
Vol 1084 ◽  
pp. 602-605 ◽  
Author(s):  
Aleksandr N. Aleinik ◽  
Aleksandr N. Baykov ◽  
Georgiy Ts. Dambaev ◽  
Evgeniy V. Semichev
Keyword(s):  
Biological Tissue ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Air Plasma ◽  
Plasma Technology ◽  
Large Application ◽  
Wet Chemical ◽  
Discharge Gap ◽  
Air Plasma Treatment

New experiments using atmospheric pressure plasma have found large application in biology and medicine. Cold air plasma treatment can be used to modify the surface of different materials for a variety of applications. The emission spectroscopy data confirmed the presence of different reactive species in the discharge gap. Surface treatments using this dry plasma technology offer an environmentally friendly alternative to the conventional wet chemical methods of microorganisms destruction, biological tissue treatment, in vitro and in vivo cell treatment. The use of cold plasma technology removes contaminants at the nanometer level.

In Vitro Killing of Clinical Fungal Strains by Low-Temperature Atmospheric-Pressure Plasma Jet

2011 ◽  
Vol 39 (2) ◽  
pp. 815-821 ◽  
Author(s):  
Georg Daeschlein ◽  
Sebastian Scholz ◽  
Thomas von Woedtke ◽  
Maria Niggemeier ◽  
Eckhard Kindel ◽  
...  
Keyword(s):  
Low Temperature ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Atmospheric Pressure Plasma Jet ◽  
Fungal Strains ◽  
Pressure Plasma

Osteogenic Potential of Non Thermal Biocompatible Atmospheric Pressure Plasma Treated Zirconia: In Vitro Study

2017 ◽  
Vol 7 (8) ◽  
pp. 662-670 ◽  
Author(s):  
Nayansi Jha ◽  
Jin Sung Choi ◽  
Ji Hye Kim ◽  
Ranju Jung ◽  
Eun Ha Choi ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
In Vitro Study ◽  
Vitro Study ◽  
Osteogenic Potential ◽  
Pressure Plasma

Antibacterial Activity of an Atmospheric Pressure Plasma Jet Against Relevant Wound Pathogens in vitro on a Simulated Wound Environment

2009 ◽  
Vol 7 (3-4) ◽  
pp. 224-230 ◽  
Author(s):  
Georg Daeschlein ◽  
Thomas von Woedtke ◽  
Eckhard Kindel ◽  
Ronny Brandenburg ◽  
Klaus-Dieter Weltmann ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Atmospheric Pressure ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Atmospheric Pressure Plasma Jet ◽  
Pressure Plasma

Perspective of strategic plasma therapy for prognostic improvement of patients with ovarian cancer

2013 ◽  
Vol 1598 ◽  
Author(s):  
Hiroaki Kajiyama ◽  
Fumi Utsumi ◽  
Kae Nakamura ◽  
Hiromasa Tanaka ◽  
Masaru Hori ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Antitumor Effect ◽  
Intraperitoneal Administration ◽  
Atmospheric Pressure Plasma ◽  
Plasma Therapy ◽  
Intracellular Mechanism ◽  
Patients Experience

ABSTRACTEpithelial ovarian carcinoma (EOC) is the leading cause of cancer-related death in women in Western countries. Once patients experience recurrence, complete cure is almost impossible. We elucidated the effect of nonequilibrium atmospheric pressure plasma on the growth of EOC, particularly in plasma-activated medium (PAM). Furthermore, we examined the role of reactive oxygen species (ROS) or their scavengers in chronic antineoplastic-resistant EOC cells. As a result, we showed PAM induced the antitumor effect of EOC cells in vitro and in vivo, even in chemoresistant cells. To apply the plasma treatment for advanced or recurrent EOC, we suggest adopting indirect plasma therapy instead of direct plasma considering intraperitoneal administration in the future. However, there are several problems under investigation, including intracellular mechanism of antitumor effect by PAM and adverse event in vivo.

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