scholarly journals Maize ZmBES1/BZR1-5 Decreases ABA Sensitivity and Confers Tolerance to Osmotic Stress in Transgenic Arabidopsis

2020 ◽  
Vol 21 (3) ◽  
pp. 996 ◽  
Author(s):  
Fuai Sun ◽  
Haoqiang Yu ◽  
Jingtao Qu ◽  
Yang Cao ◽  
Lei Ding ◽  
...  
Keyword(s):  
Stress Response ◽  
Drought Tolerance ◽  
Osmotic Stress ◽  
Transgenic Arabidopsis ◽  
Salt And Drought Tolerance ◽  
Aba Sensitivity ◽  
E Box ◽  
Mda Content ◽  
Response To Stress ◽  
Stress Related Genes

The BRI1-EMS suppressor 1 (BES1)/brassinazole-resistant 1 (BZR1) transcription factors, key components in the brassinosteroid signaling pathway, play pivotal roles in plant growth and development. However, the function of BES1/BZR1 in crops during stress response remains poorly understood. In the present study, we characterized ZmBES1/BZR1-5 from maize, which was localized to the nucleus and was responsive to abscisic acid (ABA), salt and drought stresses. Heterologous expression of ZmBES1/BZR1-5 in transgenic Arabidopsis resulted in decreased ABA sensitivity, facilitated shoot growth and root development, and enhanced salt and drought tolerance with lower malondialdehyde (MDA) content and relative electrolyte leakage (REL) under osmotic stress. The RNA sequencing (RNA-seq) analysis revealed that 84 common differentially expressed genes (DEGs) were regulated by ZmBES1/BZR1-5 in transgenic Arabidopsis. Subsequently, gene ontology and KEGG pathway enrichment analyses showed that the DEGs were enriched in response to stress, secondary metabolism and metabolic pathways. Furthermore, 30 DEGs were assigned to stress response and possessed 2–15 E-box elements in their promoters, which could be potentially recognized and bound by ZmBES1/BZR1-5. Taken together, our results reveal that the ZmBES1/BZR1-5 transcription factor positively regulates salt and drought tolerance by binding to E-box to induce the expression of downstream stress-related genes. Therefore, our study contributes to the better understanding of BES1/BZR1 function in the stress response of plants.

scholarly journals Overexpression of TaCOMT Improves Melatonin Production and Enhances Drought Tolerance in Transgenic Arabidopsis

2019 ◽  
Vol 20 (3) ◽  
pp. 652 ◽  
Author(s):  
Wen-Jing Yang ◽  
Yong-Tao Du ◽  
Yong-Bin Zhou ◽  
Jun Chen ◽  
Zhao-Shi Xu ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Triticum Aestivum L ◽  
Regulation Mechanism ◽  
Wild Type ◽  
Drought Treatment ◽  
Agricultural Applications ◽  
Transgenic Lines ◽  
Mda Content ◽  
Melatonin Production

Melatonin (N-acetyl-5-methoxytryptamine) is involved in many developmental processes and responses to various abiotic stresses in plants. Most of the studies on melatonin focus on its functions and physiological responses in plants, while its regulation mechanism remains unknown. Caffeic acid 3-O-methyltransferase (COMT) functions at a key step of the biosynthesis process of melatonin. In this study, a COMT-like gene, TaCOMT (Traes_1AL_D9035D5E0.1) was identified in common wheat (Triticum aestivum L.). Transient transformation in wheat protoplasts determined that TaCOMT is localized in cytoplasm. TaCOMT in wheat was induced by drought stress, gibberellin (GA)3 and 3-Indoleacetic acid (IAA), but not by ABA. In TaCOMT transgenic Arabidopsis, melatonin contents were higher than that in wild type (WT) plants. Under D-Mannitol treatment, the fresh weight of the transgenic Arabidopsis was significantly higher than WT, and transgenic lines had a stronger root system compared to WT. Drought tolerance assays in pots showed that the survival rate of TaCOMT-overexpression lines was significantly higher than that of WT lines. this phenotype was similar to that the WT lines treated with melatonin under drought condition. In addition, the TaCOMT transgenic lines had higher proline content and lower malondialdehyde (MDA) content compared to WT lines after drought treatment. These results indicated that overexpression of the wheat TaCOMT gene enhances drought tolerance and increases the content of melatonin in transgenic Arabidopsis. It could be one of the potential genes for agricultural applications.

The Negative Regulator OsSDS1 Significantly Reduces Salt and Drought Tolerance in Transgenic Arabidopsis

2012 ◽  
Vol 31 (3) ◽  
pp. 517-523
Author(s):  
Guoyun Xu ◽  
Mingjuan Li ◽  
Mei Huang ◽  
Yanchun Cui ◽  
Manling Wang ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Negative Regulator ◽  
Salt And Drought Tolerance

An AP2/ERF gene, IbRAP2-12, from sweetpotato is involved in salt and drought tolerance in transgenic Arabidopsis

Plant Science ◽  
2019 ◽  
Vol 281 ◽  
pp. 19-30 ◽  
Author(s):  
Yan Li ◽  
Huan Zhang ◽  
Qian Zhang ◽  
Qingchang Liu ◽  
Hong Zhai ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Transgenic Arabidopsis ◽  
Salt And Drought Tolerance

scholarly journals SlDEAD31, a Putative DEAD-Box RNA Helicase Gene, Regulates Salt and Drought Tolerance and Stress-Related Genes in Tomato

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0133849 ◽  
Author(s):  
Mingku Zhu ◽  
Guoping Chen ◽  
Tingting Dong ◽  
Lingling Wang ◽  
Jianling Zhang ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Rna Helicase ◽  
Salt And Drought Tolerance ◽  
Dead Box ◽  
Helicase Gene ◽  
Stress Related Genes
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