scholarly journals Extremely Low-Frequency Electromagnetic Fields Increase the Expression of Anagen-Related Molecules in Human Dermal Papilla Cells via GSK-3β/ERK/Akt Signaling Pathway

2020 ◽  
Vol 21 (3) ◽  
pp. 784
Author(s):  
Ga-Eun Ki ◽  
Yu-Mi Kim ◽  
Han-Moi Lim ◽  
Eun-Cheol Lee ◽  
Yun-Kyong Choi ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Electromagnetic Fields ◽  
Low Frequency ◽  
Dermal Papilla ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Androgenic Alopecia ◽  
Dermal Papilla Cells ◽  
Extremely Low Frequency ◽  
Gsk 3Β

Despite advances in medical treatments, the proportion of the population suffering from alopecia is increasing, thereby creating a need for new treatments to control hair loss and prevent balding. Human hair follicle dermal papilla cells (hDPCs), a type of specialized fibroblast in the hair bulb, play an essential role in controlling hair growth and in conditions like androgenic alopecia. This study aimed to evaluate the intensity-dependent effect of extremely low-frequency electromagnetic fields (ELF-EMFs) on the expression of anagen-related molecules in hDPCs in vitro. We examined the effect of ELF-EMF on hDPCs to determine whether activation of the GSK-3β/ERK/Akt signaling pathway improved hDPC activation and proliferation; hDPCs were exposed to ELF-EMFs at a frequency of 70 Hz and at intensities ranging from 5 to 100 G, over four days. Various PEMF intensities significantly increased the expression of anagen-related molecules, including collagen IV, laminin, ALP, and versican. In particular, an intensity of 10 G is most potent for promoting the proliferation of hDPC and expression of anagen-related molecules. Moreover, 10 G ELF-EMF significantly increased β-catenin and Wnt3α expression and GSK-3β/ERK/Akt phosphorylation. Our results confirmed that ELF-EMFs enhance hDPC activation and proliferation via the GSK-3β/ERK/Akt signaling pathway, suggesting a potential treatment strategy for alopecia.

scholarly journals Exposure to extremely low-frequency electromagnetic fields inhibits T-type calcium channels via AA/LTE4 signaling pathway

Cell Calcium ◽  
2014 ◽  
Vol 55 (1) ◽  
pp. 48-58 ◽  
Author(s):  
Yujie Cui ◽  
Xiaoyu Liu ◽  
Tingting Yang ◽  
Yan-Ai Mei ◽  
Changlong Hu
Keyword(s):  
Calcium Channels ◽  
Signaling Pathway ◽  
Electromagnetic Fields ◽  
Low Frequency ◽  
Extremely Low Frequency

scholarly journals Phosphorylated GSK‑3β protects stress‑induced apoptosis of myoblasts via the PI3K/Akt signaling pathway

2020 ◽  
Vol 22 (1) ◽  
pp. 317-327
Author(s):  
Meixi Liu ◽  
Xia Huang ◽  
Yihong Tian ◽  
Xiao Yan ◽  
Fang Wang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Gsk 3Β ◽  
Induced Apoptosis

PTEN influences insulin and lipid metabolism in bovine hepatocytes in vitro

2019 ◽  
Vol 86 (1) ◽  
pp. 73-76 ◽  
Author(s):  
Qinghua Deng ◽  
Dehui Ma ◽  
Guoquan Sun ◽  
Xue Yuan ◽  
Zhe Wang ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Insulin Sensitivity ◽  
Fatty Liver ◽  
Dairy Cows ◽  
Signaling Pathway ◽  
Fatty Acid Synthase ◽  
Akt Signaling ◽  
Negative Regulator ◽  
Akt Signaling Pathway ◽  
Gsk 3Β

AbstractDairy cows with fatty liver or ketosis display decreased insulin sensitivity and defects in the insulin receptor substrate (IRS)/PI3K/AKT signaling pathway. Phosphatase and tensin homolog (PTEN) is a well-known tumor suppressor and also a negative regulator of insulin signaling and peripheral insulin sensitivity. We investigated the hypothesis that PTEN may affect the insulin pathway-mediated hepatic glucose and lipid metabolism in dairy cows. Adenovirus vectors that over-express and silence PTEN were constructed, and then transfected into hepatocytes isolated from calves to investigate the effect of PTEN on PI3K/AKT signaling pathway. PTEN silencing increased the phosphorylation of AKT and the expression of PI3K but decreased the phosphorylation of IRS1, which increased the phosphorylation levels of glycogen synthase kinase-3β (GSK-3β) and expression of sterol regulatory element-binding protein-1c (SREBP-1c). Increased GSK-3β phosphorylation further up-regulated expression of the key enzymes phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6-Pase) involved in gluconeogenesis. Furthermore, the expression of SREBP-1c target gene fatty acid synthase (FAS) also increased significantly. We further showed that PTEN over-expression could reverse the above results. PTEN negatively regulates the enzymes involved in hepatic gluconeogenesis and lipid synthesis, which suggests that PTEN may be a therapeutic target for ketosis and fatty liver in dairy cows.

scholarly journals Combination of Paeoniflorin and Calycosin-7-glucoside Promotes Ischemic Stroke Recovery via the PI3K/AKT Signaling Pathway

2020 ◽  
Author(s):  
shengxin Wang ◽  
Xiangli Yan ◽  
Yingying He ◽  
Haozhen Zheng ◽  
PengCheng Wang ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Signaling Pathway ◽  
Therapeutic Effect ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Glucose Deprivation ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Gsk 3Β

Abstract Background Paeoniflorin (PF) and calycosin-7-glucoside (CG) play a role in protecting against brain damage following cerebral ischemia. However, the mechanism of action of PF in combination with CG (PF + CG) against ischemia/reperfusion injury remains unclear. Methods The aim of this study was to investigate the protective role of PF + CG on ischemia/reperfusion injury in vivo and in vitro, as well as its potential mechanism of action indicating that PF + CG attenuates middle cerebral artery occlusion (MCAO) /oxygen-glucose deprivation reperfusion (OGD/R) injury via the PI3K/AKT pathway. MCAO rat model was prepared by modified suture method, and behavioral scoring, cerebral infarction area, brain tissue water content measurement, using PI3K, p-PI3K, AKT, p-AKT, Bcl-2, Bax, GSK-3β protein expression as indicators, observe the effect of PI3K / AKT signaling pathway inhibitor LY294002 on the anti-ischemia-reperfusion effect of PF + CG. Oxygen deprivation method was used to prepare the OGD/R model, CCK-8 was used to determine the survival rate of HT22 cells, the contents of SOR, ROS, MDA, and LHD were determined, and apoptosis was detected by flow cytometry and mitochondrial membrane potential, using PI3K, p-PI3K, AKT, p-AKT, Bcl-2, Bax, GSK-3β protein expression as indicators, observe the effect of PI3K/AKT signaling pathway inhibitor LY294002 on the anti- oxidative and glucose deprivation effect of PF + CG. Results The animal studies showed that PF + CG significantly decreased neurobehavioral deficits, cerebral infarct volume, and brain edema; ameliorated histopathological damage in model rats; increased levels of PI3K, AKT, p-PI3K, p-AKT, and Bcl-2; and reduced BAX and GSK-3β expression. After treatment with PF + CG, the morphology and number of cells in brain tissue were restored to normal, demonstrating a therapeutic effect in cerebral ischemia-reperfusion injury. Results of further studies revealed that, in vitro, PF + CG has a therapeutic effect to enhance cell vitality; elevate levels of superoxide dismutase (SOD); reduce levels of reactive oxygen species (ROS), lactate dehydrogenase (LDH), and malondialdehyde (MDA); decrease apoptosis rate; increase levels of PI3K, AKT, p-PI3K, p-AKT, and Bcl-2; and reduce BAX and GSK-3β expression. Conclusion These results demonstrate that PF + CG has a positive therapeutic effect on ischemia/reperfusion and OGD/R injury, and the mechanism is attributed to activation of the PI3K/AKT signaling pathway.

scholarly journals Human placental extract exerts hair growth-promoting effects through the GSK-3β signaling pathway in human dermal papilla cells

2015 ◽  
Vol 36 (4) ◽  
pp. 1088-1096 ◽  
Author(s):  
TAE-RIN KWON ◽  
CHANG TAEK OH ◽  
EUN JA CHOI ◽  
HYE MIN PARK ◽  
HAE JUNG HAN ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Dermal Papilla Cells ◽  
Growth Promoting ◽  
Gsk 3Β ◽  
Placental Extract

scholarly journals PI3K/AKT Signal Pathway: A Target of Natural Products in the Prevention and Treatment of Alzheimer’s Disease and Parkinson’s Disease

2021 ◽  
Vol 12 ◽  
Author(s):  
Hui-Zhi Long ◽  
Yan Cheng ◽  
Zi-Wei Zhou ◽  
Hong-Yu Luo ◽  
Dan-Dan Wen ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Parkinson’S Disease ◽  
Alzheimer's Disease ◽  
Parkinson's Disease ◽  
Natural Products ◽  
Signaling Pathway ◽  
Glycogen Synthase ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Gsk 3Β

Alzheimer’s disease (AD) and Parkinson’s disease (PD) are two typical neurodegenerative diseases that increased with aging. With the emergence of aging population, the health problem and economic burden caused by the two diseases also increase. Phosphatidylinositol 3-kinases/protein kinase B (PI3K/AKT) signaling pathway regulates signal transduction and biological processes such as cell proliferation, apoptosis and metabolism. According to reports, it regulates neurotoxicity and mediates the survival of neurons through different substrates such as forkhead box protein Os (FoxOs), glycogen synthase kinase-3β (GSK-3β), and caspase-9. Accumulating evidences indicate that some natural products can play a neuroprotective role by activating PI3K/AKT pathway, providing an effective resource for the discovery of potential therapeutic drugs. This article reviews the relationship between AKT signaling pathway and AD and PD, and discusses the potential natural products based on the PI3K/AKT signaling pathway to treat two diseases in recent years, hoping to provide guidance and reference for this field. Further development of Chinese herbal medicine is needed to treat these two diseases.

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