scholarly journals The Probiotic BB12 Induces MicroRNAs Involved in Antigen Processing and Presentation in Porcine Monocyte-Derived Dendritic Cells

2020 ◽  
Vol 21 (3) ◽  
pp. 687
Author(s):  
Marlene Bravo-Parra ◽  
Marina Arenas-Padilla ◽  
Valeria Bárcenas-Preciado ◽  
Jesús Hernández ◽  
Verónica Mata-Haro
Keyword(s):  
Dendritic Cells ◽  
Antigen Processing ◽  
Target Genes ◽  
Regulation Of Gene Expression ◽  
Data Set ◽  
Bifidobacterium Animalis ◽  
Differentially Expressed Mirnas ◽  
Antigen Presenting ◽  
Antigen Processing And Presentation ◽  
Different Levels

MicroRNAs (miRNAs) mediate the regulation of gene expression. Several reports indicate that probiotics induce miRNA-mediated immunomodulation at different levels, such as cytokine production and the up-regulation of several markers related to antigen presentation in antigen-presenting cells. The objective of this work was to identify target genes of miRNAs that are involved in the processing and presentation of antigens in monocyte-derived dendritic cells (moDCs) stimulated with the probiotic Bifidobacterium animalis ssp. lactis BB12 (BB12). First, an in silico prediction analysis for a putative miRNA binding site within a given mRNA target was performed using RNAHybrid software with mature sequences of differentially expressed miRNAs retrieved from a Genbank data set that included BB12-stimulated and unstimulated porcine monocytes. From them, 23 genes resulted in targets of 19 miRNAs, highlighting miR-30b-3p, miR-671-5p, and miR-9858-5p, whose targets were costimulatory molecules, and were overexpressed (p < 0.05) in BB12-stimulated moDCs. The analysis of moDCs showed that the percentage of cells expressing SLA-DR+CD80+ decreased significantly (p = 0.0081) in BB12-stimulated moDCs; interleukin (IL)-10 production was unchanged at 6 h but increased after 24 h of culture in the presence of BB12 (p < 0.001). In summary, our results suggest that SLA-DR and CD80 can be down-regulated by miRNAs miR-30b-3p, miR-671-5p, and miR-9858-5p, while miR-671-5p targets IL-10.

scholarly journals Genome-Wide STAT3 Binding Analysis after Histone Deacetylase Inhibition Reveals Novel Target Genes in Dendritic Cells

2016 ◽  
Vol 9 (2) ◽  
pp. 126-144 ◽  
Author(s):  
Yaping Sun ◽  
Matthew Iyer ◽  
Richard McEachin ◽  
Meng Zhao ◽  
Yi-Mi Wu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dendritic Cells ◽  
Histone Deacetylase ◽  
Antigen Processing ◽  
Target Genes ◽  
Hdac Inhibition ◽  
Histone Deacetylase Inhibition ◽  
Immune Effector ◽  
Chip Sequencing ◽  
Genome Wide

STAT3 is a master transcriptional regulator that plays an important role in the induction of both immune activation and immune tolerance in dendritic cells (DCs). The transcriptional targets of STAT3 in promoting DC activation are becoming increasingly understood; however, the mechanisms underpinning its role in causing DC suppression remain largely unknown. To determine the functional gene targets of STAT3, we compared the genome-wide binding of STAT3 using ChIP sequencing coupled with gene expression microarrays to determine STAT3-dependent gene regulation in DCs after histone deacetylase (HDAC) inhibition. HDAC inhibition boosted the ability of STAT3 to bind to distinct DNA targets and regulate gene expression. Among the top 500 STAT3 binding sites, the frequency of canonical motifs was significantly higher than that of noncanonical motifs. Functional analysis revealed that after treatment with an HDAC inhibitor, the upregulated STAT3 target genes were those that were primarily the negative regulators of proinflammatory cytokines and those in the IL-10 signaling pathway. The downregulated STAT3-dependent targets were those involved in immune effector processes and antigen processing/presentation. The expression and functional relevance of these genes were validated. Specifically, functional studies confirmed that the upregulation of IL-10Ra by STAT3 contributed to the suppressive function of DCs following HDAC inhibition.

scholarly journals Transcriptomic microRNA Profiling of Dendritic Cells in Response to Gut Microbiota-Secreted Vesicles

Cells ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 1534
Author(s):  
Natalia Díaz-Garrido ◽  
Sarah Bonnin ◽  
Marta Riera ◽  
Rosa Gíménez ◽  
Josefa Badia ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Gut Microbiota ◽  
Target Genes ◽  
Membrane Vesicles ◽  
Fine Tuning ◽  
Differentially Expressed ◽  
Health And Nutrition ◽  
Transcriptional Regulatory ◽  
Differentially Expressed Mirnas ◽  
Key Factor

The interconnection between nutrients, metabolites and microbes is a key factor governing the healthy/pathological status of an individual. Thus, microbiota-based research is essential in order to better understand human health and nutrition. Gut bacteria release membrane vesicles (MVs) as an intercellular communication mechanism that allows the direct delivery of factors that prime the host’s innate immune system. We have previously shown that MVs from intestinal E. coli activate dendritic cells (DCs) in a strain-specific manner. To gain insights into the regulatory mechanisms involved, here, we have used an RNA deep sequencing approach to identify differentially expressed miRNAs (microRNAs) in DCs which are challenged by the MVs of the probiotic Nissle 1917 (EcN) or the commensal ECOR12. MicroRNAs are post-transcriptional regulatory mediators that permit the fine tuning of signaling pathways. This approach allowed the identification of a common set of miRNAs which are modulated by MVs from both strains and miRNAs which are differentially expressed in response to EcN or ECOR12 MVs. Based on the differential expression of the target genes and subsequent validation experiments, we correlated some of the selected miRNAs with the reported cytokine profile and specific T cell responses. As far as we know, this is the first study to analyze the regulation of miRNAs in DCs by MVs released by gut microbiota.

scholarly journals A Novel Inhibitory Receptor (ILT3) Expressed on Monocytes, Macrophages, and Dendritic Cells Involved in Antigen Processing

1997 ◽  
Vol 185 (10) ◽  
pp. 1743-1751 ◽  
Author(s):  
Marina Cella ◽  
Christian Döhring ◽  
Jacqueline Samaridis ◽  
Mark Dessing ◽  
Manfred Brockhaus ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Antigen Processing ◽  
Tyrosine Phosphatase ◽  
Immunoglobulin Superfamily ◽  
Cross Linking ◽  
Inhibitory Receptor ◽  
Cell Surface Molecule ◽  
Antigen Uptake ◽  
Inhibitory Function ◽  
Antigen Presenting

Immunoglobulin-like transcript (ILT) 3 is a novel cell surface molecule of the immunoglobulin superfamily, which is selectively expressed by myeloid antigen presenting cells (APCs) such as monocytes, macrophages, and dendritic cells. The cytoplasmic region of ILT3 contains putative immunoreceptor tyrosine-based inhibitory motifs that suggest an inhibitory function of ILT3. Indeed, co-ligation of ILT3 to stimulatory receptors expressed by APCs results in a dramatic blunting of the increased [Ca2+]i and tyrosine phosphorylation triggered by these receptors. Signal extinction involves SH2-containing protein tyrosine phosphatase 1, which is recruited by ILT3 upon cross-linking. ILT3 can also function in antigen capture and presentation. It is efficiently internalized upon cross-linking, and delivers its ligand to an intracellular compartment where it is processed and presented to T cells. Thus, ILT3 is a novel inhibitory receptor that can negatively regulate activation of APCs and can be used by APCs for antigen uptake.

scholarly journals Extracellular antigen processing and presentation by immature dendritic cells

1999 ◽  
Vol 96 (26) ◽  
pp. 15056-15061 ◽  
Author(s):  
L. Santambrogio ◽  
A. K. Sato ◽  
G. J. Carven ◽  
S. L. Belyanskaya ◽  
J. L. Strominger ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Antigen Processing ◽  
Immature Dendritic Cells ◽  
Antigen Processing And Presentation

scholarly journals Human Neonatal Dendritic Cells Are Competent in MHC Class I Antigen Processing and Presentation

PLoS ONE ◽  
2007 ◽  
Vol 2 (9) ◽  
pp. e957 ◽  
Author(s):  
Marielle C. Gold ◽  
Tammie L. Robinson ◽  
Matthew S. Cook ◽  
Laura K. Byrd ◽  
Heather D. Ehlinger ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Mhc Class I ◽  
Antigen Processing ◽  
Class I ◽  
Mhc Class I Antigen ◽  
Antigen Processing And Presentation
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