scholarly journals Citrus Auraptene Induces Glial Cell Line-Derived Neurotrophic Factor in C6 Cells

2019 ◽  
Vol 21 (1) ◽  
pp. 253 ◽  
Author(s):  
Yoshiko Furukawa ◽  
Ryu-ichi Hara ◽  
Makiko Nakaya ◽  
Satoshi Okuyama ◽  
Atsushi Sawamoto ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cell Line ◽  
Glial Cell ◽  
Neurotrophic Factor ◽  
Specific Inhibitor ◽  
C6 Glioma Cells ◽  
Dependent Manner ◽  
C6 Cells ◽  
Anti Inflammatory ◽  
The Brain

We previously demonstrated that auraptene (AUR), a natural coumarin derived from citrus plants, exerts anti-inflammatory effects in the brain, resulting in neuroprotection in some mouse models of brain disorders. The present study showed that treatment with AUR significantly increased the release of glial cell line-derived neurotrophic factor (GDNF), in a dose- and time-dependent manner, by rat C6 glioma cells, which release was associated with increased expression of GDNF mRNA. These results suggest that AUR acted as a neuroprotective agent in the brain via not only its anti-inflammatory action but also its induction of neurotrophic factor. We also showed that (1) the AUR-induced GDNF production was inhibited by U0126, a specific inhibitor of mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) 1/2, and by H89, a specific inhibitor of protein kinase A (PKA); and (2) AUR induced the phosphorylation of cAMP response element-binding protein (CREB), a transcription factor located within the nucleus. These results suggest that AUR-stimulated gdnf gene expression was up-regulated through the PKA/ERK/CREB pathway in C6 cells.

scholarly journals Glial Cell Line-derived Neurotrophic Factor Signals through the RET Receptor and Activates Mitogen-activated Protein Kinase

1996 ◽  
Vol 271 (39) ◽  
pp. 23619-23622 ◽  
Author(s):  
Carolyn A. Worby ◽  
Quinn C. Vega ◽  
Yi Zhao ◽  
Hanna H.-J. Chao ◽  
Audrey F. Seasholtz ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cell Line ◽  
Glial Cell ◽  
Neurotrophic Factor ◽  
Mitogen Activated Protein Kinase ◽  
Mitogen Activated Protein

scholarly journals Glial Cell Line-Derived Neurotrophic Factor: An Intraovarian Factor that Enhances Oocyte Developmental Competence in Vitro

Endocrinology ◽  
2007 ◽  
Vol 148 (9) ◽  
pp. 4292-4301 ◽  
Author(s):  
Katja Linher ◽  
De Wu ◽  
Julang Li
Keyword(s):  
Cell Line ◽  
Glial Cell ◽  
Neurotrophic Factor ◽  
Preimplantation Embryo ◽  
Cyclin B1 ◽  
Developmental Competence ◽  
Dependent Manner ◽  
Nuclear Maturation ◽  
Cytoplasmic Maturation

The success of early embryonic development depends on oocyte nuclear and cytoplasmic maturation. We have investigated whether glial cell line-derived neurotrophic factor (GDNF) affects the in vitro maturation (IVM) of porcine oocytes and their subsequent ability to sustain preimplantation embryo development. GDNF and both its coreceptors, GDNF family receptor α-1 (GFRα-1) and the rearranged during transformation (RET) receptor, were expressed in oocytes and their surrounding cumulus cells derived from small and large follicles. When included in IVM medium, GDNF significantly enhanced cumulus cell expansion of both small and large cumulus-oocyte complexes and increased the percentage of small follicle-derived oocytes maturing to the metaphase II stage, although nuclear maturation of large oocytes was not significantly affected. Examination of cyclin B1 protein expression as a measure of cytoplasmic maturation revealed that in the presence of GDNF, cyclin B1 levels were significantly increased in large follicle-derived oocytes, as well as in oocytes from small follicles to a level comparable to the untreated large group. After activation, a significantly higher percentage of both small and large oocytes that were matured in the presence of GDNF developed to the blastocyst stage compared with untreated controls. Indeed, GDNF enhanced the blastocyst rate of small oocytes to levels comparable to those obtained for large oocytes matured without GDNF. The effect of GDNF was specific; this was evident because its enhancement of nuclear maturation and embryo developmental potential was blocked by an antibody against GFRα-1. Our study provides the first functional evidence that GDNF affects oocyte maturation and preimplantation embryo developmental competence in a follicular stage-dependent manner. This finding may provide insights for improving the formulation of IVM culture systems, especially for oocytes from small follicles.

scholarly journals Distribution of glial cell line-derived neurotrophic factor receptor alpha-1 in the brain of adult zebrafish

2010 ◽  
pp. no-no
Author(s):  
Lucini Carla ◽  
Facello Bruna ◽  
Maruccio Lucianna ◽  
Langellotto Fernanda ◽  
Sordino Paolo ◽  
...  
Keyword(s):  
Cell Line ◽  
Glial Cell ◽  
Neurotrophic Factor ◽  
Adult Zebrafish ◽  
Receptor Alpha ◽  
Factor Receptor Alpha ◽  
The Brain
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