scholarly journals Characterization of QTLs for Seedling Resistance to Tan Spot and Septoria Nodorum Blotch in the PBW343/Kenya Nyangumi Wheat Recombinant Inbred Lines Population

2019 ◽  
Vol 20 (21) ◽  
pp. 5432 ◽  
Author(s):  
Pawan Kumar Singh ◽  
Sukhwinder Singh ◽  
Zhiying Deng ◽  
Xinyao He ◽  
Zakaria Kehel ◽  
...  
Keyword(s):  
Phenotypic Variation ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Interval Mapping ◽  
Inbred Lines ◽  
Tan Spot ◽  
Adult Plant ◽  
Septoria Nodorum ◽  
Seedling Resistance ◽  
Septoria Nodorum Blotch

Tan spot (TS) and Septoria nodorum blotch (SNB) induced by Pyrenophora tritici-repentis and Parastagonospora nodorum, respectively, cause significant yield losses and adversely affect grain quality. The objectives of this study were to decipher the genetics and map the resistance to TS and SNB in the PBW343/Kenya Nyangumi (KN) population comprising 204 F6 recombinant inbred lines (RILs). Disease screening was performed at the seedling stage under greenhouse conditions. TS was induced by P. tritici-repentis isolate MexPtr1 while SNB by P. nodorum isolate MexSN1. Segregation pattern of the RILs indicated that resistance to TS and SNB in this population was quantitative. Diversity Array Technology (DArTs) and simple sequence repeats (SSRs) markers were used to identify the quantitative trait loci (QTL) for the diseases using inclusive composite interval mapping (ICIM). Seven significant additive QTLs for TS resistance explaining 2.98 to 23.32% of the phenotypic variation were identified on chromosomes 1A, 1B, 5B, 7B and 7D. For SNB, five QTLs were found on chromosomes 1A, 5A, and 5B, explaining 5.24 to 20.87% of the phenotypic variation. The TS QTL on 1B chromosome coincided with the pleiotropic adult plant resistance (APR) gene Lr46/Yr29/Pm39. This is the first report of the APR gene Lr46/Yr29/Pm39 contributing to TS resistance.

scholarly journals A wheat chromosome 5AL region confers seedling resistance to both tan spot and Septoria nodorum blotch in two mapping populations

2019 ◽  
Vol 7 (6) ◽  
pp. 809-818 ◽  
Author(s):  
Wenjing Hu ◽  
Xinyao He ◽  
Susanne Dreisigacker ◽  
Carolina P. Sansaloni ◽  
Philomin Juliana ◽  
...  
Keyword(s):  
Wheat Chromosome ◽  
Tan Spot ◽  
Septoria Nodorum ◽  
Seedling Resistance ◽  
Septoria Nodorum Blotch ◽  
Mapping Populations

Deploying inter-specific recombinant inbred lines to map QTLs for yield-related traits in soybean

2020 ◽  
Vol 79 (04) ◽  
Author(s):  
Yashpal . ◽  
D. R. Rathod ◽  
Subhash Chandra ◽  
Anil Kumar ◽  
Raju Ratan Yadav ◽  
...  
Keyword(s):  
Recombinant Inbred ◽  
Glycine Soja ◽  
Recombinant Inbred Lines ◽  
Interval Mapping ◽  
Inbred Lines ◽  
Wild Type ◽  
Single Marker Analysis ◽  
Phenotypic Data ◽  
Single Marker ◽  
Glycine Max L

Quantitative trait loci (QTL) mapping and analyses were conducted for yield and six yield-related traits in soybean using 184 inter-specific recombinant inbred lines (RILs) derived from a cross involving wild type (Glycine soja Sieb. and Zucc.) accession DC2008-1 and cultivated (Glycine max L. Merr.) variety DS9712. A Linkage map of 1639.55 cM length was constructed with 167 SSR markers (65.65% polymorphism) with an average marker interval of 9.82 cM. Using three years phenotypic data 34 QTLs were mapped for 7 traits using Inclusive Composite Interval Mapping approach. The number of QTLs mapped for a trait varied from year to year, however, QTLs for days-to-50% flowering (qDFF5), 100-seed weight (qHSW9-1, qHSW9-2 and qHSW19) and yield (qYLD17) were mapped consistently over the three years of testing. Identified QTLs were validated through single marker analysis in 92 germplasm lines. The study demonstrated the potential of wild type soybean to harness QTLs for yield-related traits. The identified QTLs could be utilized for genetic improvement of soybean through molecular breeding.

scholarly journals Mapping QTLs for protein and oil content in soybean by removing the influence of related traits in a four-way recombinant inbred line population

2019 ◽  
Vol 157 (9-10) ◽  
pp. 659-675 ◽  
Author(s):  
Xiyu Li ◽  
Hong Xue ◽  
Kaixin Zhang ◽  
Wenbin Li ◽  
Yanlong Fang ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Recombinant Inbred ◽  
Oil Content ◽  
Molecular Breeding ◽  
Recombinant Inbred Lines ◽  
Interval Mapping ◽  
Inbred Lines ◽  
Additive Effects ◽  
Linkage Maps ◽  
Trait Locus

AbstractProtein content (PC) and oil content (OC) are important breeding traits of soybean [Glycine max (L.) Merr.]. Quantitative trait locus (QTL) mapping for PC and OC is important for molecular breeding in soybean; however, the negative correlation between PC and OC influences the accuracy of QTL mapping. In the current study, a four-way recombinant inbred lines (FW-RILs) population comprising 160 lines derived from the cross (Kenfeng14 × Kenfeng15) × (Heinong48 × Kenfeng19) was planted in eight different environments and PC and OC measured. Conditional and unconditional QTL analyses were carried out by interval mapping (IM) and inclusive complete IM based on linkage maps of 275 simple sequences repeat markers in a FW-RILs population. This analysis revealed 59 unconditional QTLs and 52 conditional QTLs among the FW-RILs. An analysis of additive effects indicated that the effects of 13 protein QTLs were not related to OC, whereas OC affected the expression of 13 and eight QTLs either partially or completely, respectively. Eight QTLs affecting OC were not influenced by PC, whereas six and 26 QTLs were partially and fully affected by PC, respectively. Among the QTLs detected in the current study, two protein QTLs and five oil QTLs had not been previously reported. These findings will facilitate marker-assisted selection and molecular breeding of soybean.

Characterization of seedling and adult plant resistance to stripe rust in recombinant inbred lines derived from wheat landrace PI388222 × Avocet cross

2020 ◽  
Vol 18 (1) ◽  
pp. 11-18
Author(s):  
Yesrab Aman ◽  
Fatima Khalid ◽  
Muzaffar Shaukat ◽  
Tariq Mahmood ◽  
Syed Wasim Hasan ◽  
...  
Keyword(s):  
Stripe Rust ◽  
Recombinant Inbred ◽  
Puccinia Striiformis ◽  
Recombinant Inbred Lines ◽  
Genetic Resistance ◽  
Inbred Lines ◽  
Adult Plant ◽  
Field Station ◽  
Washington State University ◽  
Resistant Reaction

AbstractStripe rust caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating fungal disease of wheat (Triticum aestivum L.). The best economical technique for disease control is breeding for genetic resistance to stripe rust. To find resistance genes in landrace PI388222 from Pakistan, a segregating population was developed by a cross between PI388222 and susceptible Australian spring wheat line Avocet ‘S’. The F2:4 seeds were harvested and seeds were planted in the greenhouse of Washington State University Pullman, to grow F4:5 recombinant inbred lines (RIL). A variable set of seedling reactions were noted when a set of 136 F5 and parental lines were screened with four Puccinia striiformis f. sp. tritici races (PSTv-37, PSTv-40, PSTv-4 and PSTv-51). The great proportion of RILs showed resistant reaction displayed by the RILs was against PSTv-40, for which 85% of the RILs showed resistant reaction, while less resistance to the race PSTv-37 was detected against which the resistance was for only 49% of the RILs. The RIL population was further evaluated at two locations; Palouse Conservation Field Station (PCFS) and Mount Vernon (MV). In MV field, 76% of RILs displayed resistant reaction while 15% of RILs exhibited moderate reaction. About 53% of RILs exhibited resistant reaction to four P. tritici races that were used in glasshouse screening and they were also resistant in field environments at PCFS and MV. This study demonstrates that landrace comprises partial resistance in the range of resistant to moderately resistant lines.

scholarly journals Quantitative Trait Loci Associated with Seedling Resistance to Isolates of Puccinia coronata in Oat

2003 ◽  
Vol 93 (7) ◽  
pp. 860-866 ◽  
Author(s):  
Shuquan Zhu ◽  
Kurt J. Leonard ◽  
Heidi F. Kaeppler
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Quantitative Resistance ◽  
Recombinant Inbred Lines ◽  
Interval Mapping ◽  
Field Resistance ◽  
Puccinia Coronata ◽  
Adult Plant ◽  
Seedling Resistance ◽  
Trait Loci

In our previous report, quantitative trait loci (QTL) for field adult plant resistance to crown rust were identified in an oat population of 152 F5:6 recombinant inbred lines from the cross of ‘Ogle’ (susceptible)/MAM17-5 (resistant). The objectives of the present study were to identify in the same population, the number, genomic location, and effect of QTL and digenic QTL epistasis associated with greenhouse seedling resistance to isolates of Puccinia coronata to determine if the QTL detected are isolate-specific and to compare them with previously detected QTL for field resistance. Reaction type was scored on greenhouse seedlings inoculated with three isolates. Composite interval mapping was conducted to identify genomic regions associated with resistance using a framework map of 272 molecular markers. Two QTL, Pcq1 and Pcq2, were identified for resistance to each of the three isolates. Pcq1, the major QTL controlling field resistance, did not confer detectable greenhouse seedling resistance when present singly; however, Pcq1 did serve as an enhancer of seedling resistance when it was combined with Pcq2. The final model explained 76.5, 77.9, and 79.3% of total phenotypic variation for resistance to isolates MNB248, MNB249, and MNB251, respectively. Race-specificity of quantitative resistance remains to be further examined.

scholarly journals QTL mapping for bioenergy traits in sweet sorghum recombinant inbred lines

2021 ◽  
Author(s):  
Vander Fillipe de Souza ◽  
Guilherme da Silva Pereira ◽  
Maria Marta Pastina ◽  
Rafael Augusto da Costa Parrella ◽  
Maria Lúcia Ferreira Simeone ◽  
...  
Keyword(s):  
Recombinant Inbred ◽  
Sweet Sorghum ◽  
Recombinant Inbred Lines ◽  
Genotyping By Sequencing ◽  
Interval Mapping ◽  
Inbred Lines ◽  
Biofuel Production ◽  
Soluble Solids ◽  
Nucleotide Polymorphisms ◽  
Juice Extraction

Abstract During the past decade, sweet sorghum (Sorghum bicolor Moench L.) has shown great potential for bioenergy production, especially biofuels. In this study, 223 recombinant inbred lines (RILs) derived from a cross between two sweet sorghum lines (Brandes × Wray) were evaluated in three trials. Single-nucleotide polymorphisms (SNPs) derived from genotyping by sequencing of 272 RILs were used to build a high-density genetic map comprising 3,767 SNPs spanning 1,368.83 cM. Multitrait multiple interval mapping (MT-MIM) was carried out to map quantitative trait loci (QTL) for eight bioenergy traits. A total of 33 QTLs were identified for flowering time, plant height, total soluble solids and sucrose (five QTLs each), fibers (four QTLs), and fresh biomass yield, juice extraction yield, and reducing sugars (three QTLs each). QTL hotspots were found on chromosomes 1, 3, 6, 9, and 10, in addition to other QTLs detected on chromosomes 4 and 8. We observed that 14 out of the 33 mapped QTLs were found in all three trials. Upon further development and validation in other crosses, the results provided by the present study have a great potential to be used in marker-assisted selection in sorghum breeding programs for biofuel production.

Diverse sources of resistance to Spodoptera litura (F.) in groundnut

2019 ◽  
Vol 79 (01S) ◽  
Author(s):  
M. A. Saleem ◽  
G. K. Naidu ◽  
H. L. Nadaf ◽  
P. S. Tippannavar
Keyword(s):  
Recombinant Inbred ◽  
Spodoptera Litura ◽  
Hot Spot ◽  
Recombinant Inbred Lines ◽  
Insect Pest ◽  
Inbred Lines ◽  
Leaf Damage ◽  
Botanical Variety ◽  
Resistant Genotypes ◽  
Botanical Varieties

Spodoptera litura an important insect pest of groundnut causes yield loss up to 71% in India. Though many effective chemicals are available to control Spodoptera, host plant resistance is the most desirable, economic and eco-friendly strategy. In the present study, groundnut mini core (184), recombinant inbred lines (318) and elite genotypes (44) were studied for their reaction to Spodoptera litura under hot spot location at Dharwad. Heritable component of variation existed for resistance to Spodoptera in groundnut mini core, recombinant inbred lines and elite genotypes indicating scope for selection of Spodoptera resistant genotypes. Only 29 (15%) genotypes belonging to hypogaea, fastigiata and hirsuta botanical varieties under mini core set, 15 transgressive segregants belonging to fastigiata botanical variety among 318 recombinant inbred lines and three genotypes belonging to hypogaea and fastigiata botanical varieties under elite genotypes showed resistance to Spodoptera litura with less than 10% leaf damage. Negative correlation existed between resistance to Spodoptera and days to 50 per cent flowering indicating late maturing nature of resistant genotypes. Eight resistant genotypes (ICG 862, ICG 928, ICG 76, ICG 2777, ICG 5016, ICG 12276, ICG 4412 and ICG 9905) under hypogaea botanical variety also had significantly higher pod yield. These diverse genotypes could serve as potential donors for incorporation of Spodoptera resistance in groundnut.

Sign in / Sign up

Export Citation Format

Share Document