scholarly journals Biocompatible Aloe vera and Tetracycline Hydrochloride Loaded Hybrid Nanofibrous Scaffolds for Skin Tissue Engineering

2019 ◽  
Vol 20 (20) ◽  
pp. 5174 ◽  
Author(s):  
Hariharan Ezhilarasu ◽  
Raghavendra Ramalingam ◽  
Chetna Dhand ◽  
Rajamani Lakshminarayanan ◽  
Asif Sadiq ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Aloe Vera ◽  
Dermal Fibroblasts ◽  
Tetracycline Hydrochloride ◽  
Skin Tissue ◽  
Electrospinning Technique ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds ◽  
Diffusion Assay

Aloe vera (AV) and tetracycline hydrochloride (TCH) exhibit significant properties such as anti-inflammatory, antioxidant and anti-bacterial activities to facilitate skin tissue engineering. The present study aims to develop poly-ε-caprolactone (PCL)/ AV containing curcumin (CUR), and TCH loaded hybrid nanofibrous scaffolds to validate the synergistic effect on the fibroblast proliferation and antimicrobial activity against Gram-positive and Gram-negative bacteria for wound healing. PCL/AV, PCL/CUR, PCL/AV/CUR and PCL/AV/TCH hybrid nanofibrous mats were fabricated using an electrospinning technique and were characterized for surface morphology, the successful incorporation of active compounds, hydrophilicity and the mechanical property of nanofibers. SEM revealed that there was a decrease in the fiber diameter (ranging from 360 to 770 nm) upon the addition of AV, CUR and TCH in PCL nanofibers, which were randomly oriented with bead free morphology. FTIR spectra of various electrospun samples confirmed the successful incorporation of AV, CUR and TCH into the PCL nanofibers. The fabricated nanofibrous scaffolds possessed mechanical properties within the range of human skin. The biocompatibility of electrospun nanofibrous scaffolds were evaluated on primary human dermal fibroblasts (hDF) by MTS assay, CMFDA, Sirius red and F-actin stainings. The results showed that the fabricated PCL/AV/CUR and PCL/AV/TCH nanofibrous scaffolds were non-toxic and had the potential for wound healing applications. The disc diffusion assay confirmed that the electrospun nanofibrous scaffolds possessed antibacterial activity and provided an effective wound dressing for skin tissue engineering.

scholarly journals Fabrication and Characterization of Core-Shell Electrospun Fibrous Mats Containing Medicinal Herbs for Wound Healing and Skin Tissue Engineering

Marine Drugs ◽  
2019 ◽  
Vol 17 (1) ◽  
pp. 27 ◽  
Author(s):  
Elahe Zahedi ◽  
Akbar Esmaeili ◽  
Niloofar Eslahi ◽  
Mohammad Ali Shokrgozar ◽  
Abdolreza Simchi
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Aloe Vera ◽  
Cellular Growth ◽  
Skin Tissue ◽  
Core Shell ◽  
Electron Microscopic ◽  
Electrospinning Technique ◽  
Water Contact ◽  
Skin Tissue Engineering

Nanofibrous structures mimicking the native extracellular matrix have attracted considerable attention for biomedical applications. The present study aims to design and produce drug-eluting core-shell fibrous scaffolds for wound healing and skin tissue engineering. Aloe vera extracts were encapsulated inside polymer fibers containing chitosan, polycaprolactone, and keratin using the co-axial electrospinning technique. Electron microscopic studies show that continuous and uniform fibers with an average diameter of 209 ± 47 nm were successfully fabricated. The fibers have a core-shell structure with a shell thickness of about 90 nm, as confirmed by transmission electron microscopy. By employing Fourier-transform infrared spectroscopy, the characteristic peaks of Aloe vera were detected, which indicate successful incorporation of this natural herb into the polymeric fibers. Tensile testing and hydrophilicity measurements indicated an ultimate strength of 5.3 MPa (elongation of 0.63%) and water contact angle of 89°. In-vitro biological assay revealed increased cellular growth and adhesion with the presence of Aloe vera without any cytotoxic effects. The prepared core-shell fibrous mats containing medical herbs have a great potential for wound healing applications.

Needleless electrospinning of PVP/PGS fibrous scaffolds for skin tissue engineering applications

2018 ◽  
Author(s):  
Antonios Keirouz ◽  
Giuseppino Fortunato ◽  
Anthony Callanan ◽  
Norbert Radacsi
Keyword(s):  
Tissue Engineering ◽  
Tensile Modulus ◽  
Dermal Fibroblasts ◽  
Skin Tissue ◽  
Skin Substitute ◽  
Electrospinning Technique ◽  
Skin Tissue Engineering ◽  
Needleless Electrospinning ◽  
High Concentrations

Scaffolds and implants used for tissue engineering need to be adapted for their mechanical properties with respect to their environment within the human body. Therefore, a novel composite for skin tissue engineering is presented by use of blends of Poly(vinylpyrrolidone) (PVP) and Poly(glycerol sebacate) (PGS) were fabricated via the needleless electrospinning technique. The formed PGS/PVP blends were morphologically, thermochemically and mechanically characterized. The morphology of the developed fibers related to the concentration of PGS, with high concentrations of PGS merging the fibers together plasticizing the scaffold. The tensile modulus appeared to be affected by the concentration of PGS within the blends, with an apparent decrease in the elastic modulus of the electrospun mats and an exponential increase of the elongation at break. Ultraviolet (UV) crosslinking of PGS/PVP significantly decreased and stabilized the wettability of the formed fiber mats, as indicated by contact angle measurements. In vitro examination showed good viability and proliferation of human dermal fibroblasts over the period of a week. The present findings provide important insights for tuning the elastic properties of electrospun material by incorporating this unique elastomer, as a promising future candidate for skin substitute constructs.

Nanofibrous scaffolds for skin tissue engineering and wound healing applications

2022 ◽  
pp. 645-681
Author(s):  
Guadalupe Rivero ◽  
Matthäus D. Popov Pereira da Cunha ◽  
Pablo C. Caracciolo ◽  
Gustavo A. Abraham
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds

scholarly journals Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

2020 ◽  
Author(s):  
Lucie Bacakova ◽  
Julia Pajorova ◽  
Marketa Zikmundova ◽  
Elena Filova ◽  
Petr Mikes ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds

scholarly journals Skin tissue engineering: wound healing based on stem-cell-based therapeutic strategies

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Azar Nourian Dehkordi ◽  
Fatemeh Mirahmadi Babaheydari ◽  
Mohammad Chehelgerdi ◽  
Shiva Raeisi Dehkordi
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Stem Cell ◽  
Therapeutic Strategies ◽  
Skin Tissue ◽  
Skin Tissue Engineering

Quercetin promotes human epidermal stem cell proliferation through the estrogen receptor/β-catenin/c-Myc/cyclin A2 signaling pathway

2020 ◽  
Vol 52 (10) ◽  
pp. 1102-1110
Author(s):  
Zhaodong Wang ◽  
Guangliang Zhang ◽  
Yingying Le ◽  
Jihui Ju ◽  
Ping Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Wound Healing ◽  
Estrogen Receptor ◽  
Signaling Pathway ◽  
Skin Wound ◽  
Skin Tissue ◽  
Skin Wound Healing ◽  
Skin Tissue Engineering ◽  
Cyclin A2

Abstract Skin epidermal stem cells (EpSCs) play an important role in wound healing. Quercetin is a phytoestrogen reported to accelerate skin wound healing, but its effect on EpSCs is unknown. In this study, we investigated the effect of quercetin on human EpSC proliferation and explored the underlying mechanisms. We found that quercetin at 0.1~1 μM significantly promoted EpSC proliferation and increased the number of cells in S phase. The pro-proliferative effect of quercetin on EpSCs was confirmed in cultured human skin tissue. Mechanistic studies showed that quercetin significantly upregulated the expressions of β-catenin, c-Myc, and cyclins A2 and E1. Inhibitor for β-catenin or c-Myc significantly inhibited quercetin-induced EpSC proliferation. The β-catenin inhibitor XAV-939 suppressed quercetin-induced expressions of β-catenin, c-Myc, and cyclins A2 and E1. The c-Myc inhibitor 10058-F4 inhibited the upregulation of c-Myc and cyclin A2 by quercetin. Pretreatment of EpSCs with estrogen receptor (ER) antagonist ICI182780, but not the G protein-coupled ER1 antagonist G15, reversed quercetin-induced cell proliferation and upregulation of β-catenin, c-Myc, and cyclin A2. Collectively, these results indicate that quercetin promotes EpSC proliferation through ER-mediated activation of β-catenin/c-Myc/cyclinA2 signaling pathway and ER-independent upregulation of cyclin E1 and that quercetin may accelerate skin wound healing through promoting EpSC proliferation. As EpSCs are used not only in clinic to treat skin wounds but also as seed cells in skin tissue engineering, quercetin is a useful reagent to expand EpSCs for basic research, skin wound treatment, and skin tissue engineering.

Preparation and characterization of polyhydroxybutyrate-co -hydroxyvalerate/silk fibroin nanofibrous scaffolds for skin tissue engineering

2014 ◽  
Vol 55 (4) ◽  
pp. 907-916 ◽  
Author(s):  
Caihong Lei ◽  
Hailin Zhu ◽  
Jingjing Li ◽  
Jiuming Li ◽  
Xinxing Feng ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Nanofibrous Scaffolds
Sign in / Sign up

Export Citation Format

Share Document