scholarly journals Membrane Transporters in Human Parotid Gland-Targeted Proteomics Approach

2019 ◽  
Vol 20 (19) ◽  
pp. 4825
Author(s):  
Joanna Lapczuk-Romanska ◽  
Diana Busch ◽  
Ewa Gieruszczak ◽  
Agnieszka Drozdzik ◽  
Katarzyna Piotrowska ◽  
...  
Keyword(s):  
Parotid Gland ◽  
Protein Quantification ◽  
Membrane Transporters ◽  
Protein Abundance ◽  
Human Parotid Gland ◽  
Proteomics Approach ◽  
Liquid Chromatography Tandem Mass ◽  
Bidirectional Transport ◽  
First Time ◽  
Time Information

Salivary glands provide secretory functions, including secretion of xenobiotics and among them drugs. However, there is no published information about protein abundance of drug transporters measured using reliable protein quantification methods. Therefore, mRNA expression and absolute protein content of clinically relevant ABC (n = 6) and SLC (n = 15) family member transporters in the human parotid gland, using the qRT-PCR and liquid chromatography‒tandem mass spectrometry (LC−MS/MS) method, were studied. The abundance of nearly all measured proteins ranged between 0.04 and 0.45 pmol/mg (OCT3 > MRP1 > PEPT2 > MRP4 > MATE1 > BCRP). mRNAs of ABCB1, ABCC2, ABCC3, SLC10A1, SLC10A2, SLC22A1, SLC22A5, SLC22A6, SLC22A7, SLC22A8, SLCO1A2, SLCO1B1, SLCO1B3 and SLCO2B1 were not detected. The present study provides, for the first time, information about the protein abundance of membrane transporters in the human parotid gland, which could further be used to define salivary bidirectional transport (absorption and secretion) mechanisms of endogenous compounds and xenobiotics.

scholarly journals Morphological evidence that pentagastrin regulates secretion in the human parotid gland

2012 ◽  
Vol 220 (5) ◽  
pp. 447-453 ◽  
Author(s):  
Francesco Loy ◽  
Martina Diana ◽  
Raffaella Isola ◽  
Paola Solinas ◽  
Michela Isola ◽  
...  
Keyword(s):  
Parotid Gland ◽  
Morphological Evidence ◽  
Human Parotid Gland

scholarly journals The PRIMARA study: a prospective, descriptive, observational study to review cinacalcet use in patients with primary hyperparathyroidism in clinical practice

2014 ◽  
Vol 171 (6) ◽  
pp. 727-735 ◽  
Author(s):  
P Schwarz ◽  
J J Body ◽  
J Cáp ◽  
L C Hofbauer ◽  
M Farouk ◽  
...  
Keyword(s):  
Observational Study ◽  
Primary Hyperparathyroidism ◽  
Bone Pain ◽  
Prospective Observational Study ◽  
Renal Stones ◽  
Specialist Care ◽  
Treatment Start ◽  
Clinical Profiles ◽  
First Time ◽  
Time Information

ObjectiveMedical management of primary hyperparathyroidism (PHPT) is important in patients for whom surgery is inappropriate. We aimed to describe clinical profiles of adults with PHPT receiving cinacalcet.DesignA descriptive, prospective, observational study in hospital and specialist care centres.MethodsFor patients with PHPT, aged 23–92 years, starting cinacalcet treatment for the first time, information was collected on dosing pattern, biochemistry and adverse drug reactions (ADRs). Initial cinacalcet dosage and subsequent dose changes were at the investigator's discretion.ResultsOf 303 evaluable patients with PHPT, 134 (44%) had symptoms at diagnosis (mostly bone pain (58) or renal stones (50)). Mean albumin-corrected serum calcium (ACSC) at baseline was 11.4 mg/dl (2.9 mmol/l). The reasons for prescribing cinacalcet included: surgery deemed inappropriate (35%), patient declined surgery (28%) and surgery failed or contraindicated (22%). Mean cinacalcet dose was 43.9 mg/day (s.d., 15.8) at treatment start and 51.3 mg/day (31.8) at month 12; 219 (72%) patients completed 12 months treatment. The main reason for cinacalcet discontinuation was parathyroidectomy (40; 13%). At 3, 6 and 12 months from the start of treatment, 63, 69 and 71% of patients, respectively, had an ACSC of ≤10.3 mg/dl vs 9.9% at baseline. Reductions from baseline in ACSC of ≥1 mg/dl were seen in 56, 63 and 60% of patients respectively. ADRs were reported in 81 patients (27%), most commonly nausea. A total of 7.6% of patients discontinued cinacalcet due to ADRs.ConclusionsReductions in calcium levels of ≥1 mg/dl was observed in 60% of patients 12 months after initiation of cinacalcet, without notable safety concerns.

scholarly journals Evaluation of volatile constituents, exudation of resin and occurrence of galls of Protium aracouchini (Aubl.) Marchand

2021 ◽  
Vol 5 (3) ◽  
pp. 88
Author(s):  
Thiago Augusto Araujo Correia Lima (in memorian) ◽  
Leonardo Pinto Cunha ◽  
José Eduardo Lahoz da Silva Ribeiro ◽  
Marcia Ortiz Mayo Marques ◽  
Maria da Paz Lima
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Chemical Constituents ◽  
Brazilian Amazon ◽  
High Concentration ◽  
Forest Reserve ◽  
Volatile Constituents ◽  
Aerial Parts ◽  
First Time ◽  
Time Information

Protium aracouchini (Aubl.) Marchand [sin Icica aracouchini Aubl.], which occurs in the Adolpho Ducke Forest Reserve, in the Brazilian Amazon, was evaluated for the presence of galls, for resin exudation and the composition of the essential oils from the aerial parts and the resin. The experiment to stimulate the exudation of resin from the trunk was conducted using a 2-chloroethylphosphonic acid solution. The resin produced after 40 days and the aerial parts had their essential oils extracted in a Clevenger apparatus and the volatile chemical constituents were analyzed using GC/MS. The non-oxygenated sesquiterpenes α-copaene (21.15%) and α-gurjunene (13.69%), in addition to the oxygenated sesquiterpene spathulenol (10.32%), were detected as the majority constituents of the essential oil of the leaves, and a concentration similar to that of α-gurjunene was found in the branches (13.28%). The resin essential oil showed a high concentration of hydrocarbon monoterpenes (76.49%) with a predominance of α-pinene (17.57%) and limonene (46.11%). Four gall morphotypes were found associated with this species. The present study reports for the first time information on the volatile constituents and the resinous potential of P. aracouchini, and registers the morphotypes of the galls that help in the taxonomy of the species.

Immunohistochemical localisation of substance P in human parotid gland

1992 ◽  
Vol 21 (1) ◽  
pp. 54-58 ◽  
Author(s):  
B.D. Whitley ◽  
J.W. Ferguson ◽  
A.J. Harris ◽  
T.B. Kardos
Keyword(s):  
Substance P ◽  
Parotid Gland ◽  
Human Parotid Gland

scholarly journals Analysis of the Carry-Over of Ochratoxin A from Feed to Milk, Blood, Urine, and Different Tissues of Dairy Cows Based on the Establishment of a Reliable LC-MS/MS Method

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2823 ◽  
Author(s):  
Zhiqi Zhang ◽  
Zhichen Fan ◽  
Dongxia Nie ◽  
Zhihui Zhao ◽  
Zheng Han
Keyword(s):  
Dairy Cows ◽  
Ochratoxin A ◽  
Limit Of Quantification ◽  
Fresh Milk ◽  
Liquid Chromatography Tandem Mass ◽  
Ochratoxin Α ◽  
Carry Over ◽  
First Time ◽  
Different Tissues

A rapid and reliable liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of ochratoxin A (OTA) and its metabolite ochratoxin α (OTα), for the first time, in dairy cow plasma, milk, urine, heart, liver, spleen, lung, and kidney. The established method was extensively validated by determining the linearity (R2 ≥ 0.990), sensitivity (lower limit of quantification, 0.1–0.2 ng mL−1), recovery (75.3–114.1%), precision (RSD ≤ 13.6%), and stability (≥83.0%). Based on the methodological advances, the carry-over of OTA was subsequently studied after oral administration of 30 μg/kg body weight OTA to dairy cows. As revealed, OTA and OTα were detected in urine, with maximal concentrations of 1.8 ng mL−1 and 324.6 ng mL−1, respectively, but not in milk, plasma, or different tissues, verifying the protection effects of rumen flora against OTA exposure for dairy cows. Moreover, 100 fresh milk samples randomly collected from different supermarkets in Shanghai were also analyzed, and no positive samples were found, further proving the correctness of the in vivo biotransformation results. Thus, from the currently available data, regarding OTA contamination issues on dairy cows, no significant health risks were related to OTA exposure due to the consumption of these products.

Synthesis of DABCO-based ionic liquid functionalized magnetic nanoparticles as a novel sorbent for the determination of cephalosporins in milk samples by dispersive solid-phase extraction followed by ultra-performance liquid chromatography-tandem mass spectrometry

2019 ◽  
Vol 43 (34) ◽  
pp. 13554-13570 ◽  
Author(s):  
Hamed Sahebi ◽  
Elaheh Konoz ◽  
Ali Ezabadi
Keyword(s):  
Ionic Liquid ◽  
Solid Phase ◽  
Ultra Performance Liquid Chromatography ◽  
Dispersive Solid Phase Extraction ◽  
Magnetic Iron Oxide Nanoparticles ◽  
Chromatography Tandem Mass Spectrometry ◽  
Functionalized Magnetic Nanoparticles ◽  
Liquid Chromatography Tandem Mass ◽  
First Time

In this study, DABCO-based ionic liquid-functionalized magnetic iron oxide nanoparticles were synthesized for the first time by the quaternization reaction with 1-(2,3-dihydroxypropyl)-1,4-diazabicyclo[2.2.2]octanylium chloride and chloro-functionalized Fe3O4 nanoparticles.

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