scholarly journals Genomic Organization of the B3-Domain Transcription Factor Family in Grapevine (Vitis vinifera L.) and Expression during Seed Development in Seedless and Seeded Cultivars

2019 ◽  
Vol 20 (18) ◽  
pp. 4553 ◽  
Author(s):  
Bilal Ahmad ◽  
Songlin Zhang ◽  
Jin Yao ◽  
Mati Ur Rahman ◽  
Muhammad Hanif ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Vitis Vinifera ◽  
Seed Development ◽  
Genomic Organization ◽  
Vitis Vinifera L ◽  
Limited Information ◽  
Transcription Factor Family ◽  
Cis Element ◽  
B3 Domain ◽  
Vegetative And Reproductive Growth

Members of the plant-specific B3-domain transcription factor family have important and varied functions, especially with respect to vegetative and reproductive growth. Although B3 genes have been studied in many other plants, there is limited information on the genomic organization and expression of B3 genes in grapevine (Vitis vinifera L.). In this study, we identified 50 B3 genes in the grapevine genome and analyzed these genes in terms of chromosomal location and syntenic relationships, intron–exon organization, and promoter cis-element content. We also analyzed the presumed proteins in terms of domain structure and phylogenetic relationships. Based on the results, we classified these genes into five subfamilies. The syntenic relationships suggest that approximately half of the genes resulted from genome duplication, contributing to the expansion of the B3 family in grapevine. The analysis of cis-element composition suggested that most of these genes may function in response to hormones, light, and stress. We also analyzed expression of members of the B3 family in various structures of grapevine plants, including the seed during seed development. Many B3 genes were expressed preferentially in one or more structures of the developed plant, suggesting specific roles in growth and development. Furthermore, several of the genes were expressed differentially in early developing seeds from representative seeded and seedless cultivars, suggesting a role in seed development or abortion. The results of this study provide a foundation for functional analysis of B3 genes and new resources for future molecular breeding of grapevine.

scholarly journals A R2R3-MYB Transcription Factor, VvMYBC2L2, Functions as a Transcriptional Repressor of Anthocyanin Biosynthesis in Grapevine (Vitis vinifera L.)

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 92 ◽  
Author(s):  
Ziguo Zhu ◽  
Guirong Li ◽  
Li Liu ◽  
Qingtian Zhang ◽  
Zhen Han ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Vitis Vinifera ◽  
Anthocyanin Biosynthesis ◽  
Transcriptional Repressor ◽  
Negative Regulator ◽  
Myb Transcription Factor ◽  
Vitis Vinifera L ◽  
Flavonoid Pathway ◽  
Leucoanthocyanidin Reductase ◽  
R2r3 Myb

In grapevine, the MYB transcription factors play an important role in the flavonoid pathway. Here, a R2R3-MYB transcription factor, VvMYBC2L2, isolated from Vitis vinifera cultivar Yatomi Rose, may be involved in anthocyanin biosynthesis as a transcriptional repressor. VvMYBC2L2 was shown to be a nuclear protein. The gene was shown to be strongly expressed in root, flower and seed tissue, but weakly expressed during the fruit development in grapevine. Overexpressing the VvMYBC2L2 gene in tobacco resulted in a very marked decrease in petal anthocyanin concentration. Expression analysis of flavonoid biosynthesis structural genes revealed that chalcone synthase (CHS), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin reductase (LAR) and UDP glucose flavonoid 3-O-glucosyl transferase (UFGT) were strongly down-regulated in the VvMYBC2L2-overexpressed tobacco. In addition, transcription of the regulatory genes AN1a and AN1b was completely suppressed in transgenic plants. These results suggested that VvMYBC2L2 plays a role as a negative regulator of anthocyanin biosynthesis.

Seed Development and Abortion in Vitis vinifera L., cv. Chardonnay

1996 ◽  
Vol 157 (6) ◽  
pp. 703-712 ◽  
Author(s):  
A. Ebadi ◽  
M. Sedgley ◽  
P. May ◽  
B. G. Coombe
Keyword(s):  
Vitis Vinifera ◽  
Seed Development ◽  
Vitis Vinifera L

scholarly journals Identification and Characterization of ANAC042, a Transcription Factor Family Gene Involved in the Regulation of Camalexin Biosynthesis in Arabidopsis

2012 ◽  
Vol 25 (5) ◽  
pp. 684-696 ◽  
Author(s):  
Hirohisa Saga ◽  
Takumi Ogawa ◽  
Kosuke Kai ◽  
Hideyuki Suzuki ◽  
Yoshiyuki Ogata ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Molecular Mechanisms ◽  
Kinase Inhibitor ◽  
Alternaria Brassicicola ◽  
Limited Information ◽  
Transcription Factor Family ◽  
Gus Reporter ◽  
In The Wild ◽  
Dna Insertion ◽  
Reporter Assays

Camalexin is the major phytoalexin in Arabidopsis. An almost complete set of camalexin biosynthetic enzymes have been elucidated but only limited information is available regarding molecular mechanisms regulating camalexin biosynthesis. Here, we demonstrate that ANAC042, a member of the NAM, ATAF1/2, and CUC2 (NAC) transcription factor family genes, is involved in camalexin biosynthesis induction. T-DNA insertion mutants of ANAC042 failed to accumulate camalexin at the levels achieved in the wild type, and were highly susceptible to Alternaria brassicicola infection. The camalexin biosynthetic genes CYP71A12, CYP71A13, and CYP71B15/PAD3 were not fully induced in the mutants, indicating that the camalexin defects were at least partly a result of reduced expression levels of these P450 genes. β-Glucuronidase (GUS)-reporter assays demonstrated tissue-specific induction of ANAC042 in response to differential pathogen infections. Bacterial flagellin (Flg22) induced ANAC042 expression in the root-elongation zone, the camalexin biosynthetic site, and the induction was abolished in the presence of either a general kinase inhibitor (K252a), a Ca2+-chelator (BAPTA), or methyl jasmonate. The GUS-reporter assay revealed repression of the Flg22-dependent ANAC042 expression in the ethylene-insensitive ein2-1 background but not in sid2-2 plants defective for salicylic acid biosynthesis. We discuss ANAC042 as a key transcription factor involved in previously unknown regulatory mechanisms to induce phytoalexin biosynthesis in Arabidopsis.

Does the hydrocooling of Vitis vinifera cv. Semillon vines protect the vegetative and reproductive growth processes and vine performance against high summer temperatures?

2014 ◽  
Vol 41 (6) ◽  
pp. 620 ◽  
Author(s):  
Dennis H. Greer ◽  
Mark M. Weedon
Keyword(s):  
Vitis Vinifera ◽  
Cooling System ◽  
Growing Season ◽  
Major Effect ◽  
Vitis Vinifera L ◽  
Reproductive Growth ◽  
Summer Temperatures ◽  
Vegetative And Reproductive Growth ◽  
Berry Composition ◽  
Carbon Economy

A hydrocooling system applied to Semillon (Vitis vinifera L.) grapevines as a means of protecting the vines from recurrent high temperatures. This system was assessed for impacts on vegetative and reproductive growth and development as well as for carbon economy of vines growing in vineyard conditions. The system maintained canopy temperatures at 35°C over the growing season. Leaf and bunch biomass and yield were all higher in the hydrocooled compared with control vines: the major effect was on dynamics of leaf and berry expansion. Leaf expansion was delayed and occurred over a longer duration whereas berry expansion was advanced and occurred over a longer duration than in control vines. Berry ripening was also faster in the hydrocooled vines and berries had accumulated more sugar at harvest. Leaf photosynthesis along the shoot was also higher in hydrocooled than control vines and there was a significant effect of leaf position on rates of photosynthesis of the hydrocooled vines but not with control vines. However, no differences were observed in the net shoot carbon budget. Lowered canopy temperatures were beneficial for yield and berry composition and, therefore, the cooling system warrants adoption in vineyards at risk from high temperature events during the growing season.

Negative regulation by transcription factor VvWRKY13 in drought stress of Vitis vinifera L

2020 ◽  
Vol 148 ◽  
pp. 114-121 ◽  
Author(s):  
Lixia Hou ◽  
Xinxin Fan ◽  
Jie Hao ◽  
Guangchao Liu ◽  
Zhen Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Vitis Vinifera ◽  
Negative Regulation ◽  
Vitis Vinifera L

scholarly journals Identification and functional characterisation of aquaporins in the grapevine, Vitis vinifera

2009 ◽  
Vol 36 (12) ◽  
pp. 1065 ◽  
Author(s):  
Megan C. Shelden ◽  
Susan M. Howitt ◽  
Brent N. Kaiser ◽  
Stephen D. Tyerman
Keyword(s):  
Vitis Vinifera ◽  
Water Transport ◽  
Homology Modelling ◽  
Full Length ◽  
Vitis Vinifera L ◽  
Cabernet Sauvignon ◽  
Limited Information ◽  
Transport Capacity ◽  
Plasma Membrane Intrinsic Protein ◽  
Intrinsic Protein

Plant aquaporins belong to a large superfamily of conserved proteins called the major intrinsic proteins (MIPs). There is limited information about the diversity of MIPs in grapevine, and their water transport capacity. The aim of the present study was to identify MIPs from grapevine and functionally characterise water transport of a subset of MIPs. Candidate genes were identified, by screening a Vitis vinifera L. (cv. Cabernet Sauvignon) cDNA library with gene specific probes, for aquaporin cDNAs encoding members of the plasma membrane intrinsic protein (PIP) and tonoplast intrinsic protein (TIP) subfamilies. The screen resulted in the identification of 11 full-length and two partial length aquaporin cDNAs. VvTIP2;1 isoforms had different 3′ UTRs, immediately upstream of the poly(A) tail, suggesting the presence of multiple cleavage sites for polyadenylation. Using published genome sequences of grapevine, we conducted a phylogenetic analysis of the MIPs with previously characterised MIPs from Arabidopsis. We identified 23 full-length MIP genes from the V. vinifera genome sequence of a near homozygous line (PN40024) that cluster into the four main subfamilies (and subgroups within) identified in other species. However, based on the identification of PIP2 genes in Cabernet Sauvignon that were not present in the PN40024 genome, there are likely to be more than 23 MIP genes in other heterozygous grapevine cultivars. Water transport capacity was determined for several PIPs and TIPs, by expression in Xenopus oocytes. Only VvPIP2 and VvTIP proteins function as water channels with the exception of VvPIP2;5. VvPIP2;5 differs from the water conducting VvPIP2;1 by the substitution of two highly conserved amino acids in Loop B (G97S, G100W), which was shown by homology modelling to likely form a hydrophobic block of the water pore.

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