scholarly journals High-Fat Feeding in Time-Dependent Manner Affects Metabolic Routes Leading to Nervonic Acid Synthesis in NAFLD

2019 ◽  
Vol 20 (15) ◽  
pp. 3829 ◽  
Author(s):  
Konstantynowicz-Nowicka ◽  
Berk ◽  
Chabowski ◽  
Kasacka ◽  
Bielawiec ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Lipid Accumulation ◽  
Time Dependent ◽  
Nervonic Acid ◽  
Dependent Manner ◽  
High Fat ◽  
Lipid Fractions ◽  
Fat Feeding

Nonalcoholic fatty liver disease (NAFLD) is characterized by excessive lipid accumulation in the liver. The disturbances in the fatty acid composition of stored lipids are more important than the lipid species itself, which may influence the overall effect caused by these molecules. Thus, uncovering time-dependent changes in the fatty acid composition of accumulated lipid fractions after a high fat diet seems to be a new marker of NAFLD occurrence. The experiments were conducted on high fat fed Wistar rats. The blood and liver samples were collected at the end of each experimental week and used to assess the content of lipid fractions and their fatty acid composition by gas liquid chromatography. The expression of proteins from lipid metabolism pathways and of fatty acid exporting proteins were detected by Western blotting. In the same high fat feeding period, decreased de novo lipogenesis, increased β-oxidation and lipid efflux were demonstrated. The observed effects may be the first liver protective mechanisms against lipotoxicity. Nevertheless, such effects were still not sufficient to prevent the liver from proinflammatory lipid accumulation. Moreover, the changes in liver metabolic pathways caused the plasma nervonic acid concentration in sphingomyelin to decrease simultaneously with NAFLD development, which may be a steatosis occurrence prognostic marker.

Site dependency of fatty acid composition in adipose triacylglycerol in rats and its absence as a result of high-fat feeding

Metabolism ◽  
2012 ◽  
Vol 61 (1) ◽  
pp. 92-98 ◽  
Author(s):  
Daisuke Sato ◽  
Takao Nakamura ◽  
Kazuhiko Tsutsumi ◽  
Go Shinzawa ◽  
Toru Karimata ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
High Fat ◽  
Fat Feeding ◽  
Site Dependency

scholarly journals A grape polyphenol extract modulates muscle membrane fatty acid composition and lipid metabolism in high-fat–high-sucrose diet-fed rats

2011 ◽  
Vol 106 (4) ◽  
pp. 491-501 ◽  
Author(s):  
Manar Aoun ◽  
Francoise Michel ◽  
Gilles Fouret ◽  
Audrey Schlernitzauer ◽  
Vincent Ollendorff ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Protein Expression ◽  
Acid Composition ◽  
Phospholipid Fatty Acid ◽  
Phospholipid Fatty Acid Composition ◽  
High Fat ◽  
Gene And Protein Expression ◽  
High Sucrose

Accumulation of muscle TAG content and modification of muscle phospholipid fatty acid pattern may have an impact on lipid metabolism, increasing the risk of developing diabetes. Some polyphenols have been reported to modulate lipid metabolism, in particular those issued from red grapes. The present study was designed to determine whether a grape polyphenol extract (PPE) modulates skeletal muscle TAG content and phospholipid fatty acid composition in high-fat–high-sucrose (HFHS) diet-fed rats. Muscle plasmalemmal and mitochondrial fatty acid transporters, GLUT4 and lipid metabolism pathways were also explored. The PPE decreased muscle TAG content in HFHS/PPE diet-fed rats compared with HFHS diet-fed rats and induced higher proportions of n-3 PUFA in phospholipids. The PPE significantly up-regulated GLUT4 mRNA expression. Gene and protein expression of muscle fatty acid transporter cluster of differentiation 36 (CD36) was increased in HFHS diet-fed rats but returned to control values in HFHS/PPE diet-fed rats. Carnitine palmitoyltransferase 1 protein expression was decreased with the PPE. Mitochondrial β-hydroxyacyl CoA dehydrogenase was increased in HFHS diet-fed rats and returned to control values with PPE supplementation. Lipogenesis, mitochondrial biogenesis and mitochondrial activity were not affected by the PPE. In conclusion, the PPE modulated membrane phospholipid fatty acid composition and decreased muscle TAG content in HFHS diet-fed rats. The PPE lowered CD36 gene and protein expression, probably decreasing fatty acid transport and lipid accumulation within skeletal muscle, and increased muscle GLUT4 expression. These effects of the PPE are in favour of a better insulin sensibility.

Effect of dietary n-9 eicosatrienoic acid on the fatty acid composition of plasma lipid fractions and tissue phospholipids

Lipids ◽  
1996 ◽  
Vol 31 (8) ◽  
pp. 829-837 ◽  
Author(s):  
L. G. Cleland ◽  
M. A. Neumann ◽  
R. A. Gibson ◽  
T. Hamazaki ◽  
K. Akimoto ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Plasma Lipid ◽  
Eicosatrienoic Acid ◽  
Lipid Fractions

scholarly journals Comparison of the Uptake and Processing of Cholesterol from Chylomicrons of Different Fatty Acid Composition in Rats Fed High-Fat and Low-Fat Diets

1997 ◽  
Vol 246 (1) ◽  
pp. 92-102 ◽  
Author(s):  
Elena Bravo ◽  
Loredana Flora ◽  
Alfredo Cantafora ◽  
Veronica Luca ◽  
Marco Tripodi ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
High Fat ◽  
Low Fat ◽  
Fat Diets

A 3-ketoacyl-CoA synthase 11 (KCS11) homolog from Malania oleifera synthesizes nervonic acid in plants rich in 11Z-eicosenoic acid

2020 ◽  
Author(s):  
Zhuowei Li ◽  
Shijie Ma ◽  
Huan Song ◽  
Zheng Yang ◽  
Cuizhu Zhao ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Erucic Acid ◽  
Acid Composition ◽  
Eicosenoic Acid ◽  
Nervonic Acid ◽  
Endangered Tree ◽  
Acid Accumulation ◽  
Pharmaceutical Industries

Abstract Nervonic acid (24:1) is a major component in nerve and brain tissues and it has important applications in food and pharmaceutical industries. Malania oleifera seeds contain about 40% nervonic acid. However, the mechanism of nervonic acid biosynthesis and accumulation in seeds of this endangered tree species remains unknown. In this study, developmental changes in fatty acid composition within embryos and their pericarps were investigated. Nervonic acid proportions steadily increased in developing embryos but 24:1 was not detected in pericarps at any stage. Two 3-ketoacyl-CoA synthase (KCS) homologs have been isolated from M. oleifera developing seeds by homologous cloning methods. Both KCSs are expressed in developing embryos but not detected in pericarps. Based on a phylogenetic analysis, these two KCSs were named as MoKCS4 and MoKCS11. Seed-specific expression of the MoKCS11 in Arabidopsis thaliana led to about 5% nervonic acid accumulation, while expression of the MoKCS4 did not show an obvious change in fatty acid composition. It is noteworthy that the transformation of the same MoKCS11 construct into two Brassica napus cultivars with high erucic acid did not produce the expected accumulation of nervonic acid, although expression of MoKCS11 was detected in the developing embryos of transgenic lines. In contrast, overexpression of MoKCS11 results in similar level of nervonic acid accumulation in camelina, a species which contains a similar level of 11Z-eicosenoic acid as does Arabidopsis thaliana. Taken together, the MoKCS11 may have a substrate preference for 11Z-eicosenoic acid, but not for erucic acid, in planta.

scholarly journals Impact of UV-B on drought- or cadmium-induced changes in the fatty acid composition of membrane lipid fractions in wheat

2014 ◽  
Vol 108 ◽  
pp. 129-134 ◽  
Author(s):  
Orsolya Kinga Gondor ◽  
Gabriella Szalai ◽  
Viktória Kovács ◽  
Tibor Janda ◽  
Magda Pál
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Membrane Lipid ◽  
Lipid Fractions ◽  
Induced Changes
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