scholarly journals GmFAD3A, A ω-3 Fatty Acid Desaturase Gene, Enhances Cold Tolerance and Seed Germination Rate under Low Temperature in Rice

2019 ◽  
Vol 20 (15) ◽  
pp. 3796 ◽  
Author(s):  
Xin Wang ◽  
Chao Yu ◽  
Yi Liu ◽  
Lu Yang ◽  
Yang Li ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Seed Germination ◽  
Low Temperature ◽  
Cold Tolerance ◽  
Transgenic Rice ◽  
Germination Rate ◽  
Fatty Acid Desaturase ◽  
Proline Content ◽  
Cold Sensitivity ◽  
Survival Ratio

Low temperature is an environmental stress factor that is always been applied in research on improving crop growth, productivity, and quality of crops. Polyunsaturated fatty acids (PUFAs) play an important role in cold tolerance, so its genetic manipulation of the PUFA contents in crops has led to the modification of cold sensitivity. In this study, we over-expressed an ω-3 fatty acid desaturase from Glycine max (GmFAD3A) drove by a maize ubiquitin promoter in rice. Compared to the wild type (ZH11), ectopic expression of GmFAD3A increased the contents of lipids and total PUFAs. Seed germination rates in GmFAD3A transgenic rice were enhanced under low temperature (15 °C). Moreover, cold tolerance and survival ratio were significantly improved in GmFAD3A transgenic seedlings. Malondialdehyde (MDA) content in GmFAD3A transgenic rice was lower than that in WT under cold stress, while proline content obviously increased. Meanwhile, the activities of superoxide dismutase (SOD), hydroperoxidase (CAT), and peroxidase (POD) increased substantially in GmFAD3A transgenic rice after 4 h of cold treatment. Taken together, our results suggest that GmFAD3A can enhances cold tolerance and the seed germination rate at a low temperature in rice through the accumulation of proline content, the synergistic increase of the antioxidant enzymes activity, which finally ameliorated the oxidative damage.

scholarly journals Association mapping of germinability and seedling vigor in sorghum under controlled low-temperature conditions

Genome ◽  
2016 ◽  
Vol 59 (2) ◽  
pp. 137-145 ◽  
Author(s):  
Hari D. Upadhyaya ◽  
Yi-Hong Wang ◽  
Dintyala V.S.S.R. Sastry ◽  
Sangam L. Dwivedi ◽  
P.V. Vara Prasad ◽  
...  
Keyword(s):  
Seed Germination ◽  
Low Temperature ◽  
Cold Tolerance ◽  
Germination Rate ◽  
Strong Association ◽  
Marker Locus ◽  
Seedling Vigor ◽  
Mini Core Collection ◽  
Genome Wide ◽  
Soil Temperatures

Sorghum is one of the world’s most important food, feed, and fiber crops as well as a potential feedstock for lignocellulosic bioenergy. Early-season planting extends sorghum’s growing season and increases yield in temperate regions. However, sorghum’s sensitivity to low soil temperatures adversely impacts seed germination. In this study, we evaluated the 242 accessions of the ICRISAT sorghum mini core collection for seed germination and seedling vigor at 12 °C as a measure of cold tolerance. Genome-wide association analysis was performed with approximately 162 177 single nucleotide polymorphism markers. Only one marker locus (Locus 7-2) was significantly associated with low-temperature germination and none with vigor. The linkage of Locus 7-2 to low-temperature germination was supported by four lines of evidence: strong association in three independent experiments, co-localization with previously mapped cold tolerance quantitative trait loci (QTL) in sorghum, a candidate gene that increases cold tolerance and germination rate when its wheat homolog is overexpressed in tobacco, and its syntenic region in rice co-localized with two cold tolerance QTL in rice. This locus may be useful in developing tools for molecular breeding of sorghums with improved low-temperature germinability.

scholarly journals Bioinformatics and Expression Pattern Analysis of Soybean Fatty Acid Desaturase Family Gene

2019 ◽  
Author(s):  
Nan Wu ◽  
Long Jiang ◽  
Xiaoming Yu ◽  
Xiangbo Yang ◽  
Dianyuan Chen ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Amino Acid ◽  
Low Temperature ◽  
Negative Correlation ◽  
Expression Patterns ◽  
Fatty Acid Desaturase ◽  
Oleic Acid Content ◽  
Grain Development ◽  
Response Elements

Abstract To clarify the homology and structural differences of seven genes in the soybean fatty acid desaturase 2 (FAD2) family and the relationship between the expression level of each gene and oleic acid content at different stages of grain development, the seven genes in this family were studied through informatics analysis and evaluation of expression patterns. Amino acid sequence analysis of the seven FAD2 genes in soybean showed that the genes were located on 6 chromosomes, and the number of amino acids encoded by all the genes ranged between 216 and 387. Isoelectric point analysis showed that the proteins were alkaline. Phylogenetic tree comparison results indicated that the soybean FAD2 genes have higher homology with the Arabidopsis thaliana gene and belong to the same dicotyledon, while GmFAD2-2 and GmFAD2-2a have the highest homology, and GmFAD2-1 and GmFAD2-1b have the same homology, the highest source and less homology with other soybean FAD2 genes, and their function is expected to be slightly different. Transmembrane prediction of each gene in the family showed that GmFAD2-2b contains two transmembrane regions. The remaining genes contain 3-6 different transmembrane structures, all of which are hydrophilic. The amino acid sequences were aligned, and the results showed that the genes in this family contain histidine clusters at the same position, but in GmFAD2-2b, the histidine cluster composition is different from that in other genes. According to the gene 5’ UTR intron cis-regulatory element analysis, the gene contains hormone response elements, light response elements, meristem response elements, and participates in low temperature as a cis-acting element. By analysing the expression patterns of genes in the FAD2 family under low temperature stress, it was found that GmFAD2-2 and GmFAD2-1b exhibited seed-specific expression, while the other genes were constitutively expressed. The genes were up-regulated under low temperature stress. To adapt to low temperature, plants produced more unsaturated fatty acids, and the expression of genes was different at different treatment times. In four soybean genotypes, "Jike Soybean 20", "Ji Midou 3", "Jike Midou No. 1", and "Jike Fresh Bean 1", the soybean FAD content at different stages of grain development was determined, and content was found to increase, then decrease, and finally stabilize. Correlation analysis showed that there was a negative correlation between gene expression and oleic acid content, and the correlation coefficients for GmFAD2-1 and GmFAD2-2b were the largest, showing a significant negative correlation.

Seed Germination and Physiological Characteristics of Amaranthus mangostanus L. under Drought Stress

2011 ◽  
Vol 183-185 ◽  
pp. 1071-1074
Author(s):  
Yong Dong Sun ◽  
Xiao Hua Du ◽  
Wen Jie Zhang ◽  
Li Sun ◽  
Ran Li
Keyword(s):  
Drought Stress ◽  
Seed Germination ◽  
Germination Rate ◽  
Proline Content ◽  
Shoot Length ◽  
Physiological Characteristics ◽  
Sod Activity ◽  
Peg 6000 ◽  
Mda Content ◽  
Effects Of Drought

Effects of drought stress on the seed germination and physiological characteristics of amaranth were investigated. The results were as follows: the germination rate and germination potential of amaranth decreased with the increasing of PEG-6000 concentrations. Meanwhile, the root length, shoot length and peroxidase (POD) activity were significantly increased at lower PEG-6000 concentrations, but then decreased with the increasing of PEG-6000. Malondialdehyde (MDA) content, proline content and superoxide dismutase (SOD) activity were all significantly increased under drought stress, and reached the top at 20% PEG-6000. These findings indicated that amaranth tolerates drought stress through increasing the activities of SOD and POD and accumulating proline content.

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